Abstract:
:Preservation of photosynthetic activities (photophosphorylation, electron transport, fluorescence induction, 0.3-second delayed light emission) of isolated broken (class C) chloroplasts by low temperature storage was investigated under a wide range of conditions in order to optimize long time activity retention.The more labile functions (photophosphorylation and electron transport) required very low temperatures (below -79 C) and relatively high (above 20%, v/v) concentrations of cryoprotectives for satisfactory stabilization. Fluorescence induction and delayed light emission were less sensitive, especially during the 1st month of storage.Taking into account the effect of cryoprotectives on absolute activities prior to freezing, optimum activity retention was observed with a medium containing ethylene glycol (30%, v/v) and a storage temperature of -100 C or below. In this case, given fast thawing and high chloroplast concentration, practically 100% preservation of all of the photosynthetic activities investigated was obtained for at least 10 months, even with very simple freezing and storage procedures.The same optimal medium at somewhat higher temperatures (-79 C and to a lesser extent at -41 C) caused a dramatic uncoupling effect: photophosphorylation was inhibited in a few hours, while electron transport increased 3- to 5-fold. The enhanced electron transport was stable for almost a month and then declined sharply. This uncoupling effect was specific only to ethylene glycol.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Farkas DL,Malkin Sdoi
10.1104/pp.64.6.942subject
Has Abstractpub_date
1979-12-01 00:00:00pages
942-7issue
6eissn
0032-0889issn
1532-2548journal_volume
64pub_type
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