Abstract:
:To acquire iron, many plant species reduce soil Fe(III) to Fe(II) by Fe(III)-chelate reductases embedded in the plasma membrane of root epidermal cells. The reduced product is then taken up by Fe(II) transporter proteins. These activities are induced under Fe deficiency. We describe here the FRO1 gene from pea (Pisum sativum), which encodes an Fe(III)-chelate reductase. Consistent with this proposed role, FRO1 shows similarity to other oxidoreductase proteins, and expression of FRO1 in yeast conferred increased Fe(III)-chelate reductase activity. Furthermore, FRO1 mRNA levels in plants correlated with Fe(III)-chelate reductase activity. Sites of FRO1 expression in roots, leaves, and nodules were determined. FRO1 mRNA was detected throughout the root, but was most abundant in the outer epidermal cells. Expression was detected in mesophyll cells in leaves. In root nodules, mRNA was detected in the infection zone and nitrogen-fixing region. These results indicate that FRO1 acts in root Fe uptake and they suggest a role in Fe distribution throughout the plant. Characterization of FRO1 has also provided new insights into the regulation of Fe uptake. FRO1 expression and reductase activity was detected only in Fe-deficient roots of Sparkle, whereas both were constitutive in brz and dgl, two mutants with incorrectly regulated Fe accumulation. In contrast, FRO1 expression was responsive to Fe status in shoots of all three plant lines. These results indicate differential regulation of FRO1 in roots and shoots, and improper FRO1 regulation in response to a shoot-derived signal of iron status in the roots of the brz and dgl mutants.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Waters BM,Blevins DG,Eide DJdoi
10.1104/pp.010829subject
Has Abstractpub_date
2002-05-01 00:00:00pages
85-94issue
1eissn
0032-0889issn
1532-2548journal_volume
129pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::Rubbing-induced lignification in Bryonia dioica internodes is significantly impaired by N(o-hydroxyphenyl) and N(o-aminophenyl) sulfinamoyl tertiobutyl acetate, specific inhibitors of cinnamyl alcohol dehydrogenase, an enzyme which is strictly associated with lignin monomer synthesis. Along with the reduction of ligni...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.84.1.10
更新日期:1987-05-01 00:00:00
abstract::Bicarbonate-(14)C was provided to 5- and 11-day-old suspension cultures of Paul's Scarlet rose, and the incorporation of (14)C into lipid, protein, amino acids, and organic acids was determined. The rate of bicarbonate uptake was approximately the same by 5- and 11-day-old cells, but the distribution of (14)C among ce...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.55.4.643
更新日期:1975-04-01 00:00:00
abstract::Enriched mitochondrial fractions isolated from durum wheat seedlings via differential centrifugation exhibited classical cyanide- or antimycin A-insensitive O(2) uptake which was inhibited by either salicylhydroxamic acid or propyl gallate. Further purification of this fraction using Percoll density gradients resulted...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.67.3.594
更新日期:1981-03-01 00:00:00
abstract::The pool sizes of free l-phenylalanine and l-tyrosine, the precursors of rosmarinic acid in Anchusa officinalis L. cell suspension cultures, fluctuated during the culture cycle. The major increase in pool sizes was preceded by a peak of prephenate aminotransferase activity, while the subsequent decrease coincided with...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.89.2.429
更新日期:1989-02-01 00:00:00
abstract::Chloroplasts are multifunctional organelles whose morphology is affected by environmental stresses. Although the three-dimensional (3D) architecture of thylakoid membranes has been reported previously, a 3D visualization of chloroplast under stress has not been explored. In this work, we used a positive-strand RNA ((+...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.17.00871
更新日期:2018-01-01 00:00:00
abstract::An NADPH-dependent NO2--reducing system was reconstituted in vitro using ferredoxin (Fd) NADP+ oxidoreductase (FNR), Fd, and nitrite reductase (NiR) from the green alga Chlamydomonas reinhardtii. NO2- reduction was dependent on all protein components and was operated under either aerobic or anaerobic conditions. NO2- ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.117.1.303
更新日期:1998-05-01 00:00:00
abstract::2-Cysteine peroxiredoxins (2-CPs) constitute a ubiquitous group of peroxidases that reduce cell-toxic alkyl hydroperoxides to their corresponding alcohols. Recently, we cloned 2-CP cDNAs from plants and characterized them as chloroplast proteins. To elucidate the physiological function of the 2-CP in plant metabolism,...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.119.4.1407
更新日期:1999-04-01 00:00:00
abstract::Plants contain several genes encoding thioredoxins (Trxs), small proteins involved in the regulation of the activity of many enzymes through dithiol-disulfide exchange. In addition to chloroplastic and cytoplasmic Trx systems, plant mitochondria contain a reduced nicotinamide adenine dinucleotide phosphate-dependent T...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.138073
更新日期:2009-06-01 00:00:00
abstract::The distribution of activity of a potassium-stimulated ATPase associated with the plasma membrane was determined in 4-day-old soybean roots. Changes in protein-based specific activity of the enzyme coincided with developmental changes in the root. Activity was low in the region of the root cap, increased to a maximum ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.63.6.1187
更新日期:1979-06-01 00:00:00
abstract::Responses of superoxide dismutase (EC 1.15.1.1) and glutathione reductase (EC 1.6.4.2) activities were evaluated in leaf tissue from intact cotton plants (Cotton Branch 1697) which were exposed to 75% O(2), 350 microliters per liter CO(2) for 48 hours. Soluble protein was extracted from O(2)-treated and control tissue...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.66.3.482
更新日期:1980-09-01 00:00:00
abstract::A novel mutant of Arabidopsis thaliana, arc6 (accumulation and replication of chloroplasts), has been isolated from a transfer DNA-mutagenized population of Arabidopsis seedlings. arc6 has the most extreme arc mutant phenotype we have yet described, with only one to three chloroplasts per leaf mesophyll cell compared ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.3.1169
更新日期:1994-11-01 00:00:00
abstract::The PDF1.2 gene of Arabidopsis encoding a plant defensin is commonly used as a marker for characterization of the jasmonate-dependent defense responses. Here, using PDF1.2 promoter-deletion lines linked to the beta-glucoronidase-reporter gene, we examined putative promoter elements associated with jasmonate-responsive...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.102.017814
更新日期:2003-06-01 00:00:00
abstract::Aspen (Populus tremuloides Michx.) leaf extracts contain a newly discovered enzyme activity that catalyzes the magnesium ion-dependent elimination of diphosphate from dimethylallyl diphosphate with rearrangement to form isoprene (2-methyl, 1-3-butadiene). This isoprene synthase activity has been partially purified. Th...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.97.4.1588
更新日期:1991-12-01 00:00:00
abstract::4-Hydroxyphenylpyruvate dioxygenase (4HPPD) catalyzes the formation of homogentisate (2,5-dihydroxyphenylacetate) from p-hydroxyphenylpyruvate and molecular oxygen. In plants this enzyme activity is involved in two distinct metabolic processes, the biosynthesis of prenylquinones and the catabolism of tyrosine. We repo...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.119.4.1507
更新日期:1999-04-01 00:00:00
abstract::The actin cytoskeleton is hypothesized to play a major role in gravity perception and transduction mechanisms in roots of plants. To determine whether actin microfilaments (MFs) are involved in these processes in stem-like organs, we studied gravitropism in Arabidopsis inflorescence stems and hypocotyls. Localization ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.010804
更新日期:2002-02-01 00:00:00
abstract::Two dual label methods were used to investigate kinetic variability of ribulose 1,5-bisphosphate (RuBP) carboxylase/oxygenase (EC 4.1.1.39). In addition to using [1-(14)C,5-(3)H]RuBP (method 1), we describe here the detailed assay with (14)CO(2) and [5-(3)H]RuBP (method 2), which generates [(3)H,(14)C]3-phosphoglyceri...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.75.3.645
更新日期:1984-07-01 00:00:00
abstract::Cysteine transport by tobacco cells (Nicotiana tabacum L. var. Xanthi) cultured on liquid B-5 medium was examined.Transport was linear with time or amount of tissue and had a pH optimum of 4.5. Cysteine transport over a wide concentration range was biphasic. The isotherm, for descriptive convenience, was divided into ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.60.6.807
更新日期:1977-12-01 00:00:00
abstract::Three isoforms of dihydroxyacetone phosphate reductase in extracts from Dunaliella tertiolecta have been separated by a diethylaminoethyl cellulose column chromatography with a shallow NaCl gradient. The chloroplasts contained the two major isoforms, and the third, minor form was in the cytosol. The isoforms are unsta...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.103.1.243
更新日期:1993-09-01 00:00:00
abstract::Ethylene inhibited the tuberization of etiolated potato (Solanum tuberosum L. var. Red La Soda) sprout sections cultured in vitro. Carbon dioxide did not overcome the C(2)H(4) inhibition but it was required for normal tuberization. Ethylene totally prevented root formation and development. It inhibited stolon elongati...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.57.4.480
更新日期:1976-04-01 00:00:00
abstract::Calreticulin (CRT) is a calcium-binding protein in the endoplasmic reticulum (ER) with an established role as a molecular chaper-one. An additional function in signal transduction, specifically in calcium distribution, is suggested but not proven. We have analyzed the expression pattern of Arabidopsis thaliana CRTs fo...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.114.1.29
更新日期:1997-05-01 00:00:00
abstract::The time course of abscisic acid (ABA) accumulation during water stress and of degradation following rehydration was investigated by analyzing the levels of ABA and its metabolites phaseic acid (PA) and alkalihydrolyzable conjugated ABA in excised leaf blades of Xanthium strumarium. Initial purification was by reverse...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.66.4.672
更新日期:1980-10-01 00:00:00
abstract::Biosynthesis of sucrose from triacylglycerol requires the bypass of the CO2-evolving reactions of the tricarboxylic acid (TCA) cycle. The regulation of the TCA cycle bypass during lipid mobilization was examined. Lipid mobilization in Brassica napus was initiated shortly after imbibition of the seed and proceeded unti...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.117.2.473
更新日期:1998-06-01 00:00:00
abstract::ELIPs (early light-induced proteins) are thylakoid proteins transiently induced during greening of etiolated seedlings and during exposure to high light stress conditions. This expression pattern suggests that these proteins may be involved in the protection of the photosynthetic apparatus against photooxidative damag...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.083055
更新日期:2006-08-01 00:00:00
abstract::We have described the modulation of four auxin-regulated genes during the growth cycle of suspension-cultured tobacco (Nicotiana tabacum [L.] var White Burley) cells. The genes were transiently expressed 2 to 8 h after transfer of stationary phase cells to fresh medium, during the transition from the quiescent phase o...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.102.2.513
更新日期:1993-06-01 00:00:00
abstract::Nitrate reductase (NR)-inactivating proteins from corn roots (Wf-9 x 38-11) and rice cell suspension cultures were tested against a partially purified NR obtained from corn leaves (W64A x W182E). The corn protein was purified 921-fold and the rice protein, 1,660-fold using standard purification procedures. Approximate...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.65.1.141
更新日期:1980-01-01 00:00:00
abstract::Selenium (Se) plays an indispensable role in human nutrition and has been implicated to have important health benefits, including being a cancer preventative agent. While different forms of Se vary in their anticarcinogenic efficacy, Se-methylselenocysteine (SeMSC) has been demonstrated to be one of the most effective...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.056549
更新日期:2005-05-01 00:00:00
abstract::The influence of light on asexual fruiting and mycelial growth of Phycomyces blakesleeanus Burgeff was studied by means of fruiting body counts and size measurements in cultures on solid media under varied incubation conditions. Five types of photoresponses were shown by ATCC Strain 8743a: (a) photoinduction of giant ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.51.3.570
更新日期:1973-03-01 00:00:00
abstract::Analysis of a mutation in cotton (Gossypium hirsutum), which is maternally inherited, revealed that the completely white sectors of leaves were deficient in the 70S class ribosomes, whereas the yellow sectors exhibited the same level of the latter as in the green leaves. ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.51.6.1138
更新日期:1973-06-01 00:00:00
abstract::The effect of NO2- assimilation on O2 exchange and CO2 fixation of the cyanobacterium, Synechococcus UTEX 625, was studied mass spectrometrically. Upon addition of 1 mM inorganic carbon to the medium, inorganic carbon pools developed and accelerated O2 photoreduction 5-fold when CO2 fixation was inhibited. During stea...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.4.1295
更新日期:1995-12-01 00:00:00
abstract::We found similarities between the effects of low night temperatures (5 degrees C-10 degrees C) and slowly imposed water stress on photosynthesis in grapevine (Vitis vinifera L.) leaves. Exposure of plants growing outdoors to successive chilling nights caused light- and CO(2)-saturated photosynthetic O(2) evolution to ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.121.2.675
更新日期:1999-10-01 00:00:00