Abstract:
:Three isoforms of dihydroxyacetone phosphate reductase in extracts from Dunaliella tertiolecta have been separated by a diethylaminoethyl cellulose column chromatography with a shallow NaCl gradient. The chloroplasts contained the two major isoforms, and the third, minor form was in the cytosol. The isoforms are unstable in the absence of glycerol and they are cold labile, but they may be partially reactivated at 35[deg]C. The first chloroplast form to elute from the DEAE cellulose column was the major form when the cells were grown on high NaCl and it has been referred to as the form for glycerol production for osmoregulation or "osmoregulator form." The second form increased in specific activity when inorganic phosphate was increased in the growth media to stimulate growth, and it has been given the designation for the form for glyceride synthesis, "glyceride form." The osmoregulator form was stimulated by NaCl added to the enzyme assay, but not by reduced Escherichia coli thioredoxin. The glyceride form had properties similar to the enzyme in leaf chloroplast, such as inhibition by NaCl and by fatty acyl-coenzyme A derivatives and some stimulation by dithiothreitol, uridine diphosphate galactose, cyti-dine diphosphate dipalmatoyl diglyceride, and reduced E. coli thioredoxin. Thus, Dunaliella chloroplasts have a salt-stimulated osmoregulatory form of dihydroxyacetone phosphate reductase, which seems to have a role in glycerol production, and an isoform, which may be involved in glyceride synthesis and which has properties similar to the enzyme in chloroplasts of higher plants.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Gee R,Goyal A,Byerrum RU,Tolbert NEdoi
10.1104/pp.103.1.243subject
Has Abstractpub_date
1993-09-01 00:00:00pages
243-249issue
1eissn
0032-0889issn
1532-2548pii
103/1/243journal_volume
103pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::Experiments were conducted to determine whether symbiotic bacteroids of Bradyrhizobium japonicum produce exopolysaccharide within soybean (Glycine max [L.] Merr. cv ;Lee 74') nodules. B. japonicum strains RT2, a derivative of USDA 110 with resistance to streptomycin and rifampicin, and RT176-1, a mutant deficient in e...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.79.2.445
更新日期:1985-10-01 00:00:00
abstract::The photometric method was used to test a possibility proposed recently that a new photoreceptor with maximum activity at 620 nm is involved in mediating chloroplast rotation in Mougeotia (Z Lechowski, J Bialczyk [1988] Plant Physiol 88: 189-193). The hypothesis was tested under conditions of continuous dichromatic un...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.94.1.221
更新日期:1990-09-01 00:00:00
abstract::Iron (Fe) is an essential mineral nutrient and an important factor for the composition of natural plant communities. Low Fe availability in aerated soils with neutral or alkaline pH has led to the evolution of elaborate mechanisms that extract Fe from the soil solution. In Arabidopsis (Arabidopsis thaliana), Fe is acq...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.18.00178
更新日期:2018-05-01 00:00:00
abstract::Glycolate and glyoxylate stimulated 100% to 300% the rate of oxygen evolution by Scenedesmus in the light in the absence of added carbon dioxide. This stimulation occurred either aerobically or anaerobically, and was sensitive to CMU. Aerobic dark respiration was stimulated 25% to 100% by glycolate. This phenomenon wa...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.44.1.55
更新日期:1969-01-01 00:00:00
abstract::Chloroplasts are multifunctional organelles whose morphology is affected by environmental stresses. Although the three-dimensional (3D) architecture of thylakoid membranes has been reported previously, a 3D visualization of chloroplast under stress has not been explored. In this work, we used a positive-strand RNA ((+...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.17.00871
更新日期:2018-01-01 00:00:00
abstract::The Kv-like (potassium voltage-dependent) K(+) channels at the plasma membrane, including the inward-rectifying KAT1 K(+) channel of Arabidopsis (Arabidopsis thaliana), are important targets for manipulating K(+) homeostasis in plants. Gating modification, especially, has been identified as a promising means by which ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.114.244319
更新日期:2014-10-01 00:00:00
abstract::Nitrate releases seed dormancy in Arabidopsis (Arabidopsis thaliana) Columbia accession seeds in part by reducing abscisic acid (ABA) levels. Nitrate led to lower levels of ABA in imbibed seeds when included in the germination medium (exogenous nitrate). Nitrate also reduced ABA levels in dry seeds when provided to th...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.126938
更新日期:2009-02-01 00:00:00
abstract::Superoxide dismutases (SODs) are ubiquitous metalloenzymes that catalyze the dismutation of superoxide radicals. Chloroplasts have two isozymes, copper/zinc SOD (Cu/ZnSOD) and iron SOD (FeSOD), encoded by nuclear genes. Because bryophytes are considered as the earliest land plants, they are one of the most interesting...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.107.114868
更新日期:2008-04-01 00:00:00
abstract::Salicylic acid (SA) plays a central role in plant disease resistance, and emerging evidence indicates that auxin, an essential plant hormone in regulating plant growth and development, is involved in plant disease susceptibility. GH3.5, a member of the GH3 family of early auxin-responsive genes in Arabidopsis (Arabido...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.107.106021
更新日期:2007-10-01 00:00:00
abstract::Glyceraldehyde 3-phosphate dehydrogenases (EC 1.2.1.12 and 1.2.1.13) have been purified from the seed, root, etiolated, and green shoot of peas (Pisum sativum). These enzymes are tetramers of 140,000 daltons, with subunits of 35,000 daltons. The enzymes differ in isoelectric point. The seed enzyme has a pI of 5.1, and...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.54.3.312
更新日期:1974-09-01 00:00:00
abstract::Histidine kinases (Hiks) in Synechocystis sp. PCC 6803 are involved in the transduction of signals associated with various kinds of environmental stress. To examine the potential role in thermotolerance of Hiks, we used genome microarray analysis to screen a Hik knockout library for mutations that affected the express...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.059097
更新日期:2005-07-01 00:00:00
abstract::A two-dimensional gel electrophoresis system that combines a cationic polyacrylamide gel electrophoresis at pH near neutrality with sodium dodecyl sulfate-polyacrylamide gel electrophoresis was used to analyze the spectrum of basic polypeptides that accumulate in bean (Phaseolus vulgaris) abscission zones after treatm...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.98.3.955
更新日期:1992-03-01 00:00:00
abstract::An important question concerning the role of carboxyarabinitol 1-phosphate (CA1P) metabolism in the light-dependent regulation of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activity is the extent to which CA1P is bound to Rubisco in vivo. We report here the development of an extraction procedure using a...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.2.731
更新日期:1994-06-01 00:00:00
abstract::Plants respond in complex ways to their environment, to their internal physiological status, and to the activity of other plants, pathogens, herbivores, and organisms. Plant Signaling 2000, a symposium sponsored by the Penn State Intercollege Graduate Program in Plant Physiology (May 18-20, 2000), explored the machine...
journal_title:Plant physiology
pub_type:
doi:10.1104/pp.124.2.499
更新日期:2000-10-01 00:00:00
abstract::The Golgi apparatus in plant cells consists of a large number of independent Golgi stack/trans-Golgi network/Golgi matrix units that appear to be randomly distributed throughout the cytoplasm. To study the dynamic behavior of these Golgi units in living plant cells, we have cloned a cDNA from soybean (Glycine max), Gm...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.121.4.1127
更新日期:1999-12-01 00:00:00
abstract::Experiments were designed to test whether variation in percent lysine in seed proteins could be recovered in plants regenerated from callus subjected to inhibitory levels of lysine plus threonine. Anther-derived callus was subjected to 1 millimolar lysine plus threonine for three successive passages and then once to t...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.84.2.509
更新日期:1987-06-01 00:00:00
abstract::A high affinity cAMP-binding protein (cABP II) was purified to homogeneity from wheat germ. The apparent molecular weight of cABP II, as determined from gel exclusion chromatography, is 5.2 x 10(5) (at low ionic strength) and 2.8 x 10(5) (at high ionic strength). One polypeptide subunit (molecular weight, 80,000) was ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.68.3.577
更新日期:1981-09-01 00:00:00
abstract::Calcium flux in sunflower (Helianthus annuus L. cv Russian mammoth) hypocotyl was measured with a Ca(2+) electrode as the increase or decrease in Ca(2+) in an aqueous solution (10 micromolar CaCl(2)) in contact with either the basal or apical end of 20 millimeter segments. Ca(2+) efflux was significantly higher at the...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.81.2.408
更新日期:1986-06-01 00:00:00
abstract::Regulation of photosynthetic gene expression by plastid-encoded RNA polymerase (PEP) is essential for chloroplast development. The activity of PEP largely relies on at least 12 PEP-associated proteins (PAPs) encoded in the nuclear genome of plant cells. A recent model proposed that these PAPs regulate the establishmen...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.17.00248
更新日期:2017-05-01 00:00:00
abstract::Many race- or isolate-specific disease resistance responses of plants toward pathogens (incompatible interactions) invoke hypersensitive response (HR)-like programmed cell death (PCD) and the coordinated activation of mitogen-activated protein kinases homologous with Arabidopsis (Arabidopsis thaliana) AtMPK6 and AtMPK...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.119511
更新日期:2008-06-01 00:00:00
abstract::Alternative oxidase (AOX) is encoded in small multigene families in plants. Functional analysis of the Arabidopsis (Arabidopsis thaliana) alternative oxidase 1c (AtAOX1c) promoter, an AOX gene not induced by oxidative stress, indicated that regulation of expression was complex, with the upstream promoter region contai...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.091819
更新日期:2007-04-01 00:00:00
abstract::Calcium/calmodulin-dependent protein kinase (CCaMK) has been shown to play an important role in abscisic acid (ABA)-induced antioxidant defense and enhance the tolerance of plants to drought stress. However, its downstream molecular events are poorly understood. Here, we identify a NAC transcription factor, ZmNAC84, i...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.16.00168
更新日期:2016-07-01 00:00:00
abstract::Diurnal changes in levels of selected metabolites associated with glycolysis, the C(3) cycle, C(4)-organic acids, and storage carbohydrates were analyzed in active Kalanchoë daigremontiana Crassulacean acid metabolism leaves. Three metabolic transition periods occurred each day. During the first two hours of light, ne...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.68.5.1002
更新日期:1981-11-01 00:00:00
abstract::Like other chenopods, sugarbeets (Beta vulgaris L. cv Great Western D-2) accumulate glycine betaine when salinized; this may be an adaptive response to stress. The pathway of betaine synthesis in leaves of salinized (150-200 millimolar NaCl) sugarbeet plants was investigated by supplying [(14)C]formate, phosphoryl[(14...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.71.3.692
更新日期:1983-03-01 00:00:00
abstract::Precise measurements of the minimal fluorescence yield (F(o)) and maximal fluorescence yield (F(m)) of a dark-adapted sample are prerequisites for the quantification of other fluorescence parameters. The pulse amplitude-modulated chlorophyll fluorometer (PAM 101 Chlorophyll Fluorometer, Heinz Walz, Effeltrich, Germany...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.100.1.367
更新日期:1992-09-01 00:00:00
abstract::The essential oils of peppermint (Mentha x piperita) and spearmint (Mentha spicata) are distinguished by the oxygenation position on the p-menthane ring of the constitutive monoterpenes that is conferred by two regiospecific cytochrome P450 limonene-3- and limonene-6-hydroxylases. Following hydroxylation of limonene, ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.053298
更新日期:2005-03-01 00:00:00
abstract::The effect of Co(2+) on ethylene production by mung bean (Phaseolus aureus Roxb.) and by apple tissues was studied. Co(2+), depending on concentrations applied, effectively inhibited ethylene production by both tissues. It also strongly inhibited the ethylene production induced by IAA, kinetin, IAA plus kinetin, Ca(2+...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.58.1.114
更新日期:1976-07-01 00:00:00
abstract::Many plants flower in response to seasonal changes in daylength. This response often varies between accessions of a single species. We studied the variation in photoperiod response found in the model species Arabidopsis (Arabidopsis thaliana). Seventy-two accessions were grown under six daylengths varying in 2-h inter...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.140772
更新日期:2010-01-01 00:00:00
abstract::Osmotic adjustment occurred during drought in expanded leaves of sunflowers (Helianthus annuus var Hysun 30) which had been continuously exposed to 660 microliters CO(2) per liter or had been previously acclimated to drought. The effect was greatest when the treatments were combined and was negligible in nonacclimated...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.86.4.1108
更新日期:1988-04-01 00:00:00
abstract::Extracts prepared from floral meristematic tissue of alfalfa (Medicago sativa L.) were investigated for expression of the enzyme transglutaminase in order to identify the major protein substrate for transglutaminase-directed modifications among plant proteins. The large polymorphic subunits of ribulose 1,5-bisphosphat...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.92.1.88
更新日期:1990-01-01 00:00:00