Isolation and characterization of glutathione S-transferase isozymes from sorghum.


:Two glutathione S-transferase (GST) isozymes, A1/A1 and B1/B2, were purified from etiolated, O-1,3-dioxolan-2-yl-methyl-2,2,2, -trifluoro-4'-chloroacetophenone-oxime-treated sorghum (Sorghum bicolor L. Moench) shoots. GST A1/A1, a constitutively expressed homodimer, had a subunit molecular mass of 26 kD and an isoelectric point of 4.9. GST A1/A1 exhibited high activity with 1-chloro-2, 4, dinitrobenzene (CDNB) but low activity with the chloroacetanilide herbicide metolachlor. For GST A1/A1, the random, rapid-equilibrium bireactant kinetic model provided a good description of the kinetic data for the substrates CDNB and glutathione (GSH). GST B1/B2 was a heterodimer with subunit molecular masses of 26 kD (designated the B1 subunit) and 28 kD (designated the B2 subunit) and a native isoelectric point of 4.8. GST B1/B2 exhibited low activity with CDNB and high activity with metolachlor as the substrate. The kinetics of GST B1/B2 activity with GSH and metolachlor fit a model describing a multisite enzyme having two binding sites with different affinities for these substrates. Both GST A1/A1 and GST B1/B2 exhibited GSH-conjugating activity with ethacrynic acid and GSH peroxidase activity with cumene hydroperoxide, 9-hydroperoxy-trans-10, cis-12-octadecadienoic acid and 13-hydroperoxy-cis-9, trans-11-octadecadienoic acid. Both GST A1/A1 and GST B1/B2 are glycoproteins, as indicated by their binding of concanavalin A. Polyclonal antibodies raised against GST A1/A1 exhibited cross-reactivity with the B1 subunit of GST B1/B2. Comparisons of the N-terminal amino acid sequences of the GST A1, B1, and B2 subunits with other type I theta-GSTs indicated a high degree of homology with the maize GST I subunit and a sugarcane GST.


Plant Physiol


Plant physiology


Gronwald JW,Plaisance KL




Has Abstract


1998-07-01 00:00:00












  • Functional analysis of methylthioribose kinase genes in plants.

    abstract::Through a biochemical and a genetic approach, we have identified several plant genes encoding methylthioribose (MTR) kinase, an enzyme involved in recycling of methionine through the methylthioadenosine (MTA) cycle. OsMTK1, an MTR kinase from rice (Oryza sativa), is 48.6 kD in size and shows cooperative kinetics with ...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Sauter M,Cornell KA,Beszteri S,Rzewuski G

    更新日期:2004-12-01 00:00:00

  • A methyl viologen-resistant mutant of Arabidopsis, which is allelic to ozone-sensitive rcd1, is tolerant to supplemental ultraviolet-B irradiation.

    abstract::To better understand the role of active oxygen species (AOS) in acquired resistance to increased levels of ultraviolet (UV)-B irradiation in plants, we isolated an Arabidopsis mutant that is resistant to methyl viologen, and its sensitivity to UV-B was investigated. A complementation test revealed that the obtained mu...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Fujibe T,Saji H,Arakawa K,Yabe N,Takeuchi Y,Yamamoto KT

    更新日期:2004-01-01 00:00:00

  • Urea is a product of ureidoglycolate degradation in chickpea. Purification and characterization of the ureidoglycolate urea-lyase.

    abstract::A ureidoglycolate-degrading activity was analyzed in different organs of chickpea (Cicer arietinum). Activity was detected in all the tissues analyzed, but highest levels of specific activity were found in pods, from which it has been purified and characterized. This is the first ureidoglycolate-degrading activity tha...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Muñoz A,Piedras P,Aguilar M,Pineda M

    更新日期:2001-02-01 00:00:00

  • The grapevine root-specific aquaporin VvPIP2;4N controls root hydraulic conductance and leaf gas exchange under well-watered conditions but not under water stress.

    abstract::We functionally characterized the grape (Vitis vinifera) VvPIP2;4N (for Plasma membrane Intrinsic Protein) aquaporin gene. Expression of VvPIP2;4N in Xenopus laevis oocytes increased their swelling rate 54-fold. Northern blot and quantitative reverse transcription-polymerase chain reaction analyses showed that VvPIP2;...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Perrone I,Gambino G,Chitarra W,Vitali M,Pagliarani C,Riccomagno N,Balestrini R,Kaldenhoff R,Uehlein N,Gribaudo I,Schubert A,Lovisolo C

    更新日期:2012-10-01 00:00:00

  • Emergent growth: an auxin-mediated response.

    abstract::Restoration of oxygenated conditions following 15 minutes to 2 hours of anoxia causes light-grown pea (Pisum sativum L. var. Alaska) stem segments to elongate 100 to 200% more than continuously aerated segments. This "emergent growth" response takes place in the presence of 5 mm F(-), an inhibitor of anaerobic respira...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Parrish DJ,Davies PJ

    更新日期:1977-04-01 00:00:00

  • Identification of Arabidopsis ethylene-responsive element binding factors with distinct induction kinetics after pathogen infection.

    abstract::Ethylene-responsive element binding factors (ERF) proteins are plant-specific transcription factors, many of which have been linked to stress responses. We have identified four Arabidopsis ERF genes whose expression was specifically induced by avirulent and virulent strains of the bacterial pathogen Pseudomonas syring...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Oñate-Sánchez L,Singh KB

    更新日期:2002-04-01 00:00:00

  • Modulation of Host Gene Expression during Initiation and Early Growth of Nodules in Cowpea, Vigna unguiculata (L.) Walp.

    abstract::Inoculation of 2-day-old cowpea (Vigna unguiculata [L.] Walp.) seedlings with Rhizobium fredii USDA257 results in proficient nodulation of the tap root. The most abundant nodulation occurs in a region roughly corresponding to the position of the root tip at the time of inoculation. We have examined plant gene expressi...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Trese AT,Pueppke SG

    更新日期:1990-04-01 00:00:00

  • Activation of a Reserve Pool of Photosystem II in Chlamydomonas reinhardtii Counteracts Photoinhibition.

    abstract::The effect of strong irradiance (2000 micromole photons per square meter per second) on PSII heterogeneity in intact cells of Chlamydomonas reinhardtii was investigated. Low light (LL, 15 micromole photons per square meter per second) grown C. reinhardtii are photoinhibited upon exposure to strong irradiance, and the ...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Neale PJ,Melis A

    更新日期:1990-04-01 00:00:00

  • Analysis of cell division and elongation underlying the developmental acceleration of root growth in Arabidopsis thaliana.

    abstract::To investigate the relation between cell division and expansion in the regulation of organ growth rate, we used Arabidopsis thaliana primary roots grown vertically at 20 degreesC with an elongation rate that increased steadily during the first 14 d after germination. We measured spatial profiles of longitudinal veloci...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Beemster GT,Baskin TI

    更新日期:1998-04-01 00:00:00

  • Oxidative Phosphorylation in Germinating Lettuce Seeds (Lactuca sativa) during the First Hours of Imbibition.

    abstract::Experiments with lettuce seeds during the first hours of imbibition showed that oxygen is necessary to sustain high adenine nucleotide ratios and consequently, energy charge values are higher than 0.8 as is usually the case in normally metabolizing tissues.The energy charge value (0.2) of dry seeds soaked in aerated w...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Hourmant A,Pradet A

    更新日期:1981-09-01 00:00:00

  • It's complicated: intraroot system variability of respiration and morphological traits in four deciduous tree species.

    abstract::Within branched root systems, a distinct heterogeneity of traits exists. Knowledge about the ecophysiology of different root types is critical to understand root system functioning. Classification schemes have to match functional root types as closely as possible to be used for sampling and modeling. Among ecophysiolo...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Rewald B,Rechenmacher A,Godbold DL

    更新日期:2014-10-01 00:00:00

  • BIG Regulates Dynamic Adjustment of Circadian Period in Arabidopsis thaliana.

    abstract::Circadian clocks drive rhythms with a period near 24 h, but the molecular basis of the regulation of the period of the circadian clockis poorly understood. We previously demonstrated that metabolites affect the free-running period of the circadian oscillator of Arabidopsis (Arabidopsis thaliana), with endogenous sugar...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Hearn TJ,Marti Ruiz MC,Abdul-Awal SM,Wimalasekera R,Stanton CR,Haydon MJ,Theodoulou FL,Hannah MA,Webb AAR

    更新日期:2018-09-01 00:00:00

  • Carbon, hydrogen, and oxygen isotope ratios of cellulose from plants having intermediary photosynthetic modes.

    abstract::Carbon and hydrogen isotope ratios of cellulose nitrate and oxygen isotope ratios of cellulose from species of greenhouse plants having different photosynthetic modes were determined. When hydrogen isotope ratios are plotted against carbon isotope ratios, four clusters of points are discernible, each representing diff...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Sternberg LO,Deniro MJ,Ting IP

    更新日期:1984-01-01 00:00:00

  • Properties of a Partially Purified Nucleoside Triphosphatase (NTPase) from the Chloroplast Envelope of Pea.

    abstract::The Mg-nucleoside triphosphatase activity associated with the inner envelope membrane of the pea chloroplast is comprised of at least two components, a major activity that is sensitive to vanadate and sodium fluoride and a minor insensitive activity. The vanadate/fluoride sensitive activity has been partially purified...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: McCarty DR,Selman BR

    更新日期:1986-04-01 00:00:00

  • Decreased cell wall digestibility in canola transformed with chimeric tyrosine decarboxylase genes from opium poppy

    abstract::Tyrosine decarboxylase (TYDC) is a common plant enzyme involved in the biosynthesis of numerous secondary metabolites, including hydroxycinnamic acid amides. Although a definite function has not yet been determined, amides have been proposed to form a physical barrier against pathogens because they are usually found a...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Facchini PJ,Yu M,Penzes-Yost C

    更新日期:1999-07-01 00:00:00

  • Induction of the arginine decarboxylase ADC2 gene provides evidence for the involvement of polyamines in the wound response in Arabidopsis.

    abstract::Polyamines are small ubiquitous molecules that have been involved in nearly all developmental processes, including the stress response. Nevertheless, no direct evidence of a role of polyamines in the wound response has been described. We have studied the expression of genes involved in polyamine biosynthesis in respon...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Perez-Amador MA,Leon J,Green PJ,Carbonell J

    更新日期:2002-11-01 00:00:00

  • The Chickpea Early Flowering 1 (Efl1) Locus Is an Ortholog of Arabidopsis ELF3.

    abstract::In climates that experience short growing seasons due to drought, heat, or end-of-season frost, early flowering is a highly desirable trait for chickpea (Cicer arietinum). In this study, we mapped, sequenced, and characterized Early flowering3 (Efl3), an ortholog of Arabidopsis (Arabidopsis thaliana) EARLY FLOWERING3 ...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Ridge S,Deokar A,Lee R,Daba K,Macknight RC,Weller JL,Tar'an B

    更新日期:2017-10-01 00:00:00

  • Plasma membrane H+-ATPase is involved in auxin-mediated cell elongation during wheat embryo development.

    abstract::Previous investigations suggested that specific auxin spatial distribution due to auxin movements to particular embryonic regions was important for normal embryonic pattern formation. To gain information on the molecular mechanism(s) by which auxin acts to direct pattern formation in specific embryonic regions, the ro...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Rober-Kleber N,Albrechtová JT,Fleig S,Huck N,Michalke W,Wagner E,Speth V,Neuhaus G,Fischer-Iglesias C

    更新日期:2003-03-01 00:00:00

  • Electron Transport Controls Glutamine Synthetase Activity in the Facultative Heterotrophic Cyanobacterium Synechocystis sp. PCC 6803.

    abstract::Glutamine synthetase (GS) from Synechocystis sp. PCC 6803 was inactivated in vivo by transferring cells from light to darkness or by incubation with the photosynthetic inhibitor 3-(3,4-dichlorophenyl)-1,1-dimethylurea but not with 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone. Addition of glucose prevented both dark...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Reyes JC,Crespo JL,Garcia-Dominguez M,Florencio FJ

    更新日期:1995-11-01 00:00:00

  • Enzymes of the Poly-beta-Hydroxybutyrate and Citric Acid Cycles of Rhizobium japonicum Bacteroids.

    abstract::The activities of several enzymes of the citric acid and poly-beta-hydroxybutyrate cycles were measured in Rhizobium japonicum 3I1B-143 bacteroids which had been isolated from soybean nodules by sucrose gradient centrifugation. During the period of developing nitrogenase activity, the specific activity of fumarase, hy...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Karr DB,Waters JK,Suzuki F,Emerich DW

    更新日期:1984-08-01 00:00:00

  • Activation of Chloroplast NADP-linked Glyceraldehyde-3-Phosphate Dehydrogenase by the Ferredoxin/Thioredoxin System.

    abstract::NADP-glyceraldehyde-3-P dehydrogenase of spinach (Spinacia oleracea) chloroplasts was activated by thioredoxin that was reduced either photochemically with ferredoxin and ferredoxin-thioredoxin reductase or chemically with dithiothreitol. The activation process that was observed with the soluble protein fraction from ...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Wolosiuk RA,Buchanan BB

    更新日期:1978-04-01 00:00:00

  • First evidence for polyamine conjugation mediated by an enzymic activity in plants.

    abstract::An enzyme activity, found for the first time in plants, mainly located in the 22,000g supernatant of the crude extract of sprout apices of Helianthus tuberosus L. cv OB1 tubers, is able in vitro to covalently bind polyamines to endogenous substrates of different molecular weights. The major assay parameters, such as p...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Serafini-Fracassini D,Del Duca S,D'Orazi D

    更新日期:1988-07-01 00:00:00

  • Abscisic acid deficiency causes changes in cuticle permeability and pectin composition that influence tomato resistance to Botrytis cinerea.

    abstract::A mutant of tomato (Solanum lycopersicum) with reduced abscisic acid (ABA) production (sitiens) exhibits increased resistance to the necrotrophic fungus Botrytis cinerea. This resistance is correlated with a rapid and strong hydrogen peroxide-driven cell wall fortification response in epidermis cells that is absent in...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Curvers K,Seifi H,Mouille G,de Rycke R,Asselbergh B,Van Hecke A,Vanderschaeghe D,Höfte H,Callewaert N,Van Breusegem F,Höfte M

    更新日期:2010-10-01 00:00:00

  • Ectopic expression of constitutively activated RACB in barley enhances susceptibility to powdery mildew and abiotic stress.

    abstract::Small RAC/ROP-family G proteins regulate development and stress responses in plants. Transient overexpression and RNA interference experiments suggested that the barley (Hordeum vulgare) RAC/ROP protein RACB is involved in susceptibility to the powdery mildew fungus Blumeria graminis f. sp. hordei. We created transgen...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Schultheiss H,Hensel G,Imani J,Broeders S,Sonnewald U,Kogel KH,Kumlehn J,Hückelhoven R

    更新日期:2005-09-01 00:00:00

  • The mechanic state of "inner tissue" in the growing zone of sunflower hypocotyls and the regulation of its growth rate following excision.

    abstract::Spontaneous growth of isolated inner tissue from the etiolated sunflower (Helianthus annuus L.) hypocotyl growing zone was investigated. A new preparation technique allowed measurements starting 3 s after excision. Elongation with respect to the turgescent and plasmolized state was quantified in terms of relative grow...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Peters WS,Tomos AD

    更新日期:2000-06-01 00:00:00

  • Effects of changing stomatal width in a red pine forest on soil water content, leaf water potential, bole diameter, and growth.

    abstract::Spraying a 16 meter tall stand of red pine (Pinus resinosa Ait.) with 10(-3)m phenylmercuric acetate in early June and again in mid-July resulted in the water use between June 1 and October 25 being reduced by almost 10%. It was demonstrated that this was caused by an increase in the leaf resistance with partial stoma...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Turner NC,Waggoner PE

    更新日期:1968-06-01 00:00:00

  • State 1-State 2 Transitions in a Unicellular Green Algae : Analysis of In Vivo Chlorophyll Fluorescence Induction Curves in the Presence of 3-(3,4-Dichlorophenyl)-1, 1-dimethylurea (DCMU).

    abstract::A study has been made on the State 1-State 2 transitions exhibited by the unicellular green algae Chlorella pyrenoidosa. Chlorophyll fluorescence induction curves from algae adapted to State 1 or State 2 have been analyzed and a comparison made with similar curves produced by decreasing the intensity of light going to...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Hodges M,Barber J

    更新日期:1983-08-01 00:00:00

  • An Arabidopsis prenylated Rab acceptor 1 isoform, AtPRA1.B6, displays differential inhibitory effects on anterograde trafficking of proteins at the endoplasmic reticulum.

    abstract::Prenylated Rab acceptors (PRAs), members of the Ypt-interacting protein family of small membrane proteins, are thought to aid the targeting of prenylated Rabs to their respective endomembrane compartments. In plants, the Arabidopsis (Arabidopsis thaliana) PRA1 family contains 19 members that display varying degrees of...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Lee MH,Jung C,Lee J,Kim SY,Lee Y,Hwang I

    更新日期:2011-10-01 00:00:00

  • A Mucin-Like Protein of Planthopper Is Required for Feeding and Induces Immunity Response in Plants.

    abstract::The brown planthopper, Nilaparvata lugens, is a pest that threatens rice (Oryza sativa) production worldwide. While feeding on rice plants, planthoppers secrete saliva, which plays crucial roles in nutrient ingestion and modulating plant defense responses, although the specific functions of salivary proteins remain la...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Shangguan X,Zhang J,Liu B,Zhao Y,Wang H,Wang Z,Guo J,Rao W,Jing S,Guan W,Ma Y,Wu Y,Hu L,Chen R,Du B,Zhu L,Yu D,He G

    更新日期:2018-01-01 00:00:00

  • Specificities of the Acyl-Acyl Carrier Protein (ACP) Thioesterase and Glycerol-3-Phosphate Acyltransferase for Octadecenoyl-ACP Isomers (Identification of a Petroselinoyl-ACP Thioesterase in Umbelliferae).

    abstract::This study was designed to address the question: How specific for double bond position and conformation are plant enzymes that act on oleoyl-acyl carrier protein (ACP)? Octadecenoyl-ACPs with cis double bonds at positions [delta]6, [delta]7, [delta]8, [delta]9, [delta]10, [delta]11, or [delta]12 and elaidyl (18:1[delt...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Dormann P,Frentzen M,Ohlrogge JB

    更新日期:1994-03-01 00:00:00