Abstract:
:Two glutathione S-transferase (GST) isozymes, A1/A1 and B1/B2, were purified from etiolated, O-1,3-dioxolan-2-yl-methyl-2,2,2, -trifluoro-4'-chloroacetophenone-oxime-treated sorghum (Sorghum bicolor L. Moench) shoots. GST A1/A1, a constitutively expressed homodimer, had a subunit molecular mass of 26 kD and an isoelectric point of 4.9. GST A1/A1 exhibited high activity with 1-chloro-2, 4, dinitrobenzene (CDNB) but low activity with the chloroacetanilide herbicide metolachlor. For GST A1/A1, the random, rapid-equilibrium bireactant kinetic model provided a good description of the kinetic data for the substrates CDNB and glutathione (GSH). GST B1/B2 was a heterodimer with subunit molecular masses of 26 kD (designated the B1 subunit) and 28 kD (designated the B2 subunit) and a native isoelectric point of 4.8. GST B1/B2 exhibited low activity with CDNB and high activity with metolachlor as the substrate. The kinetics of GST B1/B2 activity with GSH and metolachlor fit a model describing a multisite enzyme having two binding sites with different affinities for these substrates. Both GST A1/A1 and GST B1/B2 exhibited GSH-conjugating activity with ethacrynic acid and GSH peroxidase activity with cumene hydroperoxide, 9-hydroperoxy-trans-10, cis-12-octadecadienoic acid and 13-hydroperoxy-cis-9, trans-11-octadecadienoic acid. Both GST A1/A1 and GST B1/B2 are glycoproteins, as indicated by their binding of concanavalin A. Polyclonal antibodies raised against GST A1/A1 exhibited cross-reactivity with the B1 subunit of GST B1/B2. Comparisons of the N-terminal amino acid sequences of the GST A1, B1, and B2 subunits with other type I theta-GSTs indicated a high degree of homology with the maize GST I subunit and a sugarcane GST.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Gronwald JW,Plaisance KLdoi
10.1104/pp.117.3.877subject
Has Abstractpub_date
1998-07-01 00:00:00pages
877-92issue
3eissn
0032-0889issn
1532-2548journal_volume
117pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::Transgenic Nicotiana plumbaginifolia plants that express either a 5-fold increase or a 20-fold decrease in nitrate reductase (NR) activity were used to study the relationships between carbon and nitrogen metabolism in leaves. Under saturating irradiance the maximum rate of photosynthesis, per unit surface area, was de...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.1.171
更新日期:1994-01-01 00:00:00
abstract::Soybean seedlings were examined for the presence of mitochondrial tRNA. Tyrosyl transfer tRNAs from whole cells, from a well characterized mitochondrial preparation, and from a snake venom phosphodiesterase-treated mitochondrial preparation, were compared by reverse phase chromatography. It was concluded that none of ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.50.2.298
更新日期:1972-08-01 00:00:00
abstract::Rhizobia secrete nodulation (Nod) factors, which set in motion the formation of nitrogen-fixing root nodules on legume host plants. Nod factors induce several cellular responses in root hair cells within minutes, but also are essential for the formation of infection threads by which rhizobia enter the root. Based on s...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.107.100495
更新日期:2007-09-01 00:00:00
abstract::The FAD7 gene is expressed preferentially in the chlorophyllous tissues of unwounded plants. Wounding activates the expression of the FAD7 gene not only in chlorophyllous tissues, but also in nonchlorophyllous tissues of stems and roots. Our previous study suggested that wound-responsive transcriptional activation by ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.121.4.1239
更新日期:1999-12-01 00:00:00
abstract::The interaction of tropisms is important in determining the final growth form of the plant body. In roots, gravitropism is the predominant tropistic response, but phototropism also plays a role in the oriented growth of roots in flowering plants. In blue or white light, roots exhibit negative phototropism that is medi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.013847
更新日期:2003-03-01 00:00:00
abstract::We report the molecular cloning and sequence of the cDNA coding for the biotin-containing subunit of the chloroplastic acetylcoenzyme A (CoA) carboxylase (ACCase) of Arabidopsis thaliana (CAC1). The 3' end of the CAC1 sequence, coding for a peptide of 94 amino acids, which includes a putative biotinylation motif, was ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.2.619
更新日期:1995-10-01 00:00:00
abstract::Environmental stresses frequently decrease plant fertility. In Arabidopsis, the effect of salt stress on reproduction was examined using plants grown in hydroponic medium. Salt stress inhibited microsporogenesis and stamen filament elongation. Because plants grown in hydroponic media can be rapidly and transiently str...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.043091
更新日期:2004-08-01 00:00:00
abstract::The Ussing-Theorell equation, which provides a fundamental test for the independent passive movement of ions under conditions of nonequilibrium, has been used to assess the active and passive components of K(+) uptake by segments of pea epicotyl (Pisum sativum L. cultivar Alaska), incubated for 24 hours in both 1-fold...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.45.2.133
更新日期:1970-02-01 00:00:00
abstract::The relationship between net photosynthesis and CO(2) concentration was investigated for four species of lichen using an infrared gas analyzer operating in a closed loop system. All species showed a linear relationship at low CO(2) levels (100 microliters per liter) with CO(2) saturation levels being in excess of 400 ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.68.1.199
更新日期:1981-07-01 00:00:00
abstract::The Marquillo x Kenya Farmer 1 "grass-clump" dwarf selection of Triticum aestivum L. was grown under continuous 2000 foot candle light and several regimes of alternating 16 degrees and 26 degrees temperatures combined in total cycle lengths of 6, 12, 24, or 48 hr. Plants at 26 degrees grew as normal wheat. Those expos...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.44.12.1701
更新日期:1969-12-01 00:00:00
abstract::The Arabidopsis (Arabidopsis thaliana) orthologs of Brca2, a protein whose mutations are involved in breast cancer in humans, were previously shown to be essential at meiosis. In an attempt to better understand the Brca2-interacting properties, we examined four partners of the two isoforms of Brca2 identified in Arabi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.075838
更新日期:2006-03-01 00:00:00
abstract::Flooding is a frequently occurring environmental stress that can severely affect plant growth. This study shows that treatment of corn (Zea mays L.) seedlings with abscisic acid (ABA) increases their tolerance to anoxia 10-fold over untreated seedlings and twofold over seedlings treated with water. Corn seedlings stre...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.97.2.593
更新日期:1991-10-01 00:00:00
abstract::Serine hydroxymethyltransferase from mammalian and bacterial sources is a pyridoxal-5'-phosphate-containing enzyme, but the requirement of pyridoxal-5'-phosphate for the activity of the enzyme from plant sources is not clear. The specific activity of serine hydroxymethyltransferase isolated from mung bean (Vigna radia...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.95.2.351
更新日期:1991-02-01 00:00:00
abstract::Spores of the fern Onoclea sensibilis L. normally germinate to produce two cells of unequal size. The larger cell divides to produce the familiar heart-shaped prothallus. The smaller cell elongates and differentiates into the rhizoid but normally does not divide again. Onoclea spores germinate in complete darkness. Da...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.63.6.984
更新日期:1979-06-01 00:00:00
abstract::Polysaccharide analyses of mutants link several of the glycosyltransferases encoded by the 10 CesA genes of Arabidopsis to cellulose synthesis. Features of those mutant phenotypes point to particular genes depositing cellulose predominantly in either primary or secondary walls. We used transformation with antisense co...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.010931
更新日期:2002-06-01 00:00:00
abstract::It was previously reported that the hypernodulating soybean (Glycine max [L.] Merr.) mutants, derived from the cultivar Williams, had higher root concentration of isoflavonoid compounds (daidzein, genistein, and coumestrol) than did Williams at 9 to 12 days after inoculation with Bradyrhizobium japonicum. These compou...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.96.4.1277
更新日期:1991-08-01 00:00:00
abstract::Enhancement phenomena in photosynthesis of the diatom Phaeodactylum tricornutum Lewin were studied by means of a Haxo oxygen electrode and 2 monochromatic light beams. It was necessary to correct for a minor non-linearity in rate oxygen evolution vs. intensity similar to that reported for Chlorella. Action spectra on ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.43.12.1991
更新日期:1968-12-01 00:00:00
abstract::Calcium/calmodulin-dependent protein kinase (CCaMK) has been shown to play an important role in abscisic acid (ABA)-induced antioxidant defense and enhance the tolerance of plants to drought stress. However, its downstream molecular events are poorly understood. Here, we identify a NAC transcription factor, ZmNAC84, i...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.16.00168
更新日期:2016-07-01 00:00:00
abstract::Roots of Fe-sufficient and Fe-Deficient pea (Pisum sativum L.) were studied to determine the effect of Fe-deficiency on the activity of the root-cell plasmalemma Fe(2+) transport protein. Rates of Fe(III) reduction and short-term Fe(2+) influx were sequentially determined in excised primary lateral roots using Fe(III)...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.94.3.1353
更新日期:1990-11-01 00:00:00
abstract::Synthesis of nitrate reductase (EC 1.6.6.1) in Chlorella vulgaris was studied under inducing conditions, i.e. with cells grown on ammonia and then transferred to nitrate medium. Cycloheximide (but not chloramphenicol) completely inhibited synthesis of the enzyme, but only if it was added at the start (i.e. at the time...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.65.5.939
更新日期:1980-05-01 00:00:00
abstract::Glucosinolates (GSLs) are secondary metabolites whose degradation products confer intrinsic flavors and aromas to Brassicaceae vegetables. Several structures of GSLs are known in the Brassicaceae, and the biosynthetic pathway and regulatory networks have been elucidated in Arabidopsis (Arabidopsis thaliana). GSLs are ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.16.01814
更新日期:2017-03-01 00:00:00
abstract::Correlating measurements from differential scanning calorimetry, freeze-fracture freeze-etch electron microscopy, and survival of twigs after two-step cooling experiments, we provide strong evidence that winter-hardened Populus balsamifera v. virginiana (Sarg.) resists the stresses of freezing below -28 degrees C by a...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.79.1.41
更新日期:1985-09-01 00:00:00
abstract::Investigation on the biosynthesis of S-methyl-L-cysteine in radish leaves has shown that it is formed by the methylation of cysteine. This conclusion is based on: A) the relatively high recovery of radioactivity in methylcysteine sulfoxide after the administration of cysteine or methyl-labeled methionine to radish lea...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.41.8.1301
更新日期:1966-10-01 00:00:00
abstract::Euglena gracilis (1224-5/9) contains phosphoenolpyruvate carboxykinase when grown autotrophic with CO(2) in the light. Its yield is higher when an additional carbon source like glucose has been added. The enzyme is lacking in cells provided with CO(2) alone and kept in the dark, whereas highest yields result if both g...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.86.2.457
更新日期:1988-02-01 00:00:00
abstract::A 108-fold purification of the sucrose synthetase from the extract of the shoot of bamboo Lelaba oldhami was achieved by ammonium sulfate fractionation, calcium phosphate gel adsorption, and chromatographic separations on Sephadex G-100 and diethylaminoethyl-cellulose columns. Some properties of this enzyme, namely th...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.60.1.17
更新日期:1977-07-01 00:00:00
abstract::Like many microalgae, Chlamydomonas reinhardtii forms lipid droplets rich in triacylglycerols when nutrient deprived. To begin studying the mechanisms underlying this process, nitrogen (N) deprivation was used to induce triacylglycerol accumulation and changes in developmental programs such as gametogenesis. Comparati...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.110.165159
更新日期:2010-12-01 00:00:00
abstract::ATP sulfurylase and 5'-adenylylsulfate (APS) reductase catalyze two reactions in the sulfate assimilation pathway. Cell fractionation of Arabidopsis leaves revealed that ATP sulfurylase isoenzymes exist in the chloroplast and the cytosol, whereas APS reductase is localized exclusively in chloroplasts. During developme...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.124.2.715
更新日期:2000-10-01 00:00:00
abstract::Chloroplast NADPH-thioredoxin reductase (NTRC) belongs to the thioredoxin systems that control crucial metabolic and regulatory pathways in plants. Here, by characterization of T-DNA insertion lines of NTRC gene, we uncover a novel connection between chloroplast thiol redox regulation and the control of photoperiodic ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.133777
更新日期:2009-03-01 00:00:00
abstract::Chronic exposure of hybrid poplar (Populus deltoides x trichocarpa) plants to low concentrations of ozone had negative impact upon net photosynthetic capacity, dark respiration, and leaf chlorophyll contents. Exposure to as much as 0.20 microliters per liter O(3) had no immediate effect on net photosynthesis (P(n)), b...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.73.2.291
更新日期:1983-10-01 00:00:00
abstract::Isolated maize (Zea mays var. kelvedon glory) etioplasts have been used to investigate the relationships between the spectral shifts and ultrastructural changes which occur during light-induced chloroplast development. After primary photoconversion, the Shibata shift was observed as a change from 680 to 670 nm in the ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.55.2.393
更新日期:1975-02-01 00:00:00