Isolation and characterization of glutathione S-transferase isozymes from sorghum.


:Two glutathione S-transferase (GST) isozymes, A1/A1 and B1/B2, were purified from etiolated, O-1,3-dioxolan-2-yl-methyl-2,2,2, -trifluoro-4'-chloroacetophenone-oxime-treated sorghum (Sorghum bicolor L. Moench) shoots. GST A1/A1, a constitutively expressed homodimer, had a subunit molecular mass of 26 kD and an isoelectric point of 4.9. GST A1/A1 exhibited high activity with 1-chloro-2, 4, dinitrobenzene (CDNB) but low activity with the chloroacetanilide herbicide metolachlor. For GST A1/A1, the random, rapid-equilibrium bireactant kinetic model provided a good description of the kinetic data for the substrates CDNB and glutathione (GSH). GST B1/B2 was a heterodimer with subunit molecular masses of 26 kD (designated the B1 subunit) and 28 kD (designated the B2 subunit) and a native isoelectric point of 4.8. GST B1/B2 exhibited low activity with CDNB and high activity with metolachlor as the substrate. The kinetics of GST B1/B2 activity with GSH and metolachlor fit a model describing a multisite enzyme having two binding sites with different affinities for these substrates. Both GST A1/A1 and GST B1/B2 exhibited GSH-conjugating activity with ethacrynic acid and GSH peroxidase activity with cumene hydroperoxide, 9-hydroperoxy-trans-10, cis-12-octadecadienoic acid and 13-hydroperoxy-cis-9, trans-11-octadecadienoic acid. Both GST A1/A1 and GST B1/B2 are glycoproteins, as indicated by their binding of concanavalin A. Polyclonal antibodies raised against GST A1/A1 exhibited cross-reactivity with the B1 subunit of GST B1/B2. Comparisons of the N-terminal amino acid sequences of the GST A1, B1, and B2 subunits with other type I theta-GSTs indicated a high degree of homology with the maize GST I subunit and a sugarcane GST.


Plant Physiol


Plant physiology


Gronwald JW,Plaisance KL




Has Abstract


1998-07-01 00:00:00












  • Effects of ethylene on auxin transport.

    abstract::The effect of ethylene on the uptake, distribution and polar transport of C(14) from indole-3-acetic acid-2-C(14) and naphthalene acetic acid-1-C(14) in tissue sections was studied. Test species were cotton (Gossypium hirsutum, L.) and cowpea (Vigna sinensis, Endl.). Generally, incubation of tissue or intact plants wi...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Morgan PW,Gausman HW

    更新日期:1966-01-01 00:00:00

  • Kinetic characterization of reduced pyridine nucleotide dehydrogenases (duroquinone-dependent) in cucurbita microsomes.

    abstract::Some properties of microsomal electron transfer chains, dependent for oxidase activity on addition of NADH or NADPH, duroquinone, and oxygen (L. De Luca et al., 1984, Plant Sci Lett 36: 93-98) are described. Activity is characterized by negatively cooperative kinetics toward reduced pyridine nucleotides, with limiting...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Pupillo P,Valenti V,De Luca L,Hertel R

    更新日期:1986-02-01 00:00:00

  • Association of Phosphatidylinositol Kinase, Phosphatidylinositol Monophosphate Kinase, and Diacylglycerol Kinase with the Cytoskeleton and F-Actin Fractions of Carrot (Daucus carota L.) Cells Grown in Suspension Culture : Response to Cell Wall-Degrading E

    abstract::Phosphatidylinositol kinase (PI), phosphatidylinositol monophosphate (PIP) kinase, and diacylglycerol (DAG) kinase activities were detected in the cytoskeletal fraction isolated from microsomes and plasma membranes of carrot (Daucus carota L.) cells grown in suspension culture. The lipid kinase activities were associa...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Tan Z,Boss WF

    更新日期:1992-12-01 00:00:00

  • Characterization of FRO1, a pea ferric-chelate reductase involved in root iron acquisition.

    abstract::To acquire iron, many plant species reduce soil Fe(III) to Fe(II) by Fe(III)-chelate reductases embedded in the plasma membrane of root epidermal cells. The reduced product is then taken up by Fe(II) transporter proteins. These activities are induced under Fe deficiency. We describe here the FRO1 gene from pea (Pisum ...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Waters BM,Blevins DG,Eide DJ

    更新日期:2002-05-01 00:00:00

  • Cold hardening in citrus stems.

    abstract::Stem cold hardening developed to different levels in citrus types tested in controlled environments. Exotherms indicated ice spread was more uniform and rapid in unhardened than in cold-hardened stems. All attempts to inhibit the functioning of citrus leaves resulted in less cold hardening in the stems. Citrus leaves ...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Yelenosky G

    更新日期:1975-10-01 00:00:00

  • Sodium and potassium absorption by bean stem tissue.

    abstract::The effect of various periods of pretreatment in CaSO(4) solutions (aging) on the absorption of Na and K by bean stem slices was investigated. Freshly sliced tissue absorbed Na over the entire range of concentrations studied (0.02-50 mm). Potassium absorption by fresh tissue was nil at concentrations below 0.5 mm but ...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Rains DW

    更新日期:1969-04-01 00:00:00

  • Role of the Cotyledons in the Phototropic Response of Lavatera cretica Seedlings.

    abstract::Young seedlings of Lavatera cretica L. exhibit positive phototropism. The hypocotyl perceives unilateral illumination with blue light and curves towards the light source by unequal growth. In addition, the cotyledonary laminas perceive the vectorial component of unilateral illumination with blue light and reorient nor...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Schwartz A,Koller D

    更新日期:1980-07-01 00:00:00

  • Rhythmic Differences in the Basipetal Movement of Indoleacetic Acid between Separated Upper and Lower Halves of Geotropically Stimulated Corn Coleoptiles.

    abstract::Rhythmic fluctuation in the basipetal movement of auxin occurs in corn (Zea mays) coleoptiles oriented either in the vertical or in the horizontal position. This periodicity of transport rate varies from region to region in a horizontal coleoptile. Between an upper and lower half coleoptile (with respect to gravity), ...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Shen-Miller J

    更新日期:1973-08-01 00:00:00

  • A role for the GCC-box in jasmonate-mediated activation of the PDF1.2 gene of Arabidopsis.

    abstract::The PDF1.2 gene of Arabidopsis encoding a plant defensin is commonly used as a marker for characterization of the jasmonate-dependent defense responses. Here, using PDF1.2 promoter-deletion lines linked to the beta-glucoronidase-reporter gene, we examined putative promoter elements associated with jasmonate-responsive...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Brown RL,Kazan K,McGrath KC,Maclean DJ,Manners JM

    更新日期:2003-06-01 00:00:00

  • Loss-of-function mutation of REDUCED WALL ACETYLATION2 in Arabidopsis leads to reduced cell wall acetylation and increased resistance to Botrytis cinerea.

    abstract::Nearly all polysaccharides in plant cell walls are O-acetylated, including the various pectic polysaccharides and the hemicelluloses xylan, mannan, and xyloglucan. However, the enzymes involved in the polysaccharide acetylation have not been identified. While the role of polysaccharide acetylation in vivo is unclear, ...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Manabe Y,Nafisi M,Verhertbruggen Y,Orfila C,Gille S,Rautengarten C,Cherk C,Marcus SE,Somerville S,Pauly M,Knox JP,Sakuragi Y,Scheller HV

    更新日期:2011-03-01 00:00:00

  • Abscisic Acid Content, Transpiration, and Stomatal Conductance As Related to Leaf Age in Plants of Xanthium strumarium L.

    abstract::Among the four uppermost leaves of greenhouse-grown plants of Xanthium strumarium L. the content of abscisic acid per unit fresh or dry weight was highest in the youngest leaf and decreased gradually with increasing age of the leaves. Expressed per leaf, the second youngest leaf was richest in ABA; the amount of ABA p...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Raschke K,Zeevaart JA

    更新日期:1976-08-01 00:00:00

  • Senescence is induced in individually darkened Arabidopsis leaves, but inhibited in whole darkened plants.

    abstract::It has long been known that leaf senescence can be induced in many plant species by detaching leaves and placing them in the darkness. It recently has been shown that entire Arabidopsis plants placed in the darkness are not induced to senesce, as judged by visible yellowing and certain molecular markers. Here, we show...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Weaver LM,Amasino RM

    更新日期:2001-11-01 00:00:00

  • NPH4, a conditional modulator of auxin-dependent differential growth responses in Arabidopsis.

    abstract::Although sessile in nature, plants are able to use a number of mechanisms to modify their morphology in response to changing environmental conditions. Differential growth is one such mechanism. Despite its importance in plant development, little is known about the molecular events regulating the establishment of diffe...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Stowe-Evans EL,Harper RM,Motchoulski AV,Liscum E

    更新日期:1998-12-01 00:00:00

  • Degree of C(4) Photosynthesis in C(4) and C(3)-C(4)Flaveria Species and Their Hybrids : II. Inhibition of Apparent Photosynthesis by a Phosphoenolpyruvate Carboxylase Inhibitor.

    abstract::Hybrids have been made between species of Flaveria exhibiting varying levels of C(4) photosynthesis. The degree of C(4) photosynthesis expressed in four interspecific hybrids (Flaveria trinervia [C(4)] x F. linearis [C(3)-C(4)], F. brownii [C(4)-like] x F. linearis, and two three-species hybrids from F. trinervia x [F...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Brown RH,Byrd GT,Black CC

    更新日期:1992-10-01 00:00:00

  • Stomatal Responses to Light and Drought Stress in Variegated Leaves of Hedera helix.

    abstract::Direct and indirect mechanisms underlying the light response of stomata were studied in variegated leaves of the juvenile phase of Hedera helix L. Dose response curves of leaf conductance were measured with blue and red light in leaves kept in normal or in an inverted position. In the green portions of the leaves, the...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Aphalo PJ,Sánchez RA

    更新日期:1986-07-01 00:00:00

  • Influx of na, k, and ca into roots of salt-stressed cotton seedlings : effects of supplemental ca.

    abstract::High Na(+) concentrations may disrupt K(+) and Ca(2+) transport and interfere with growth of many plant species, cotton (Gossypium hirsutum L.) included. Elevated Ca(2+) levels often counteract these consequences of salinity. The effect of supplemental Ca(2+) on influx of Ca(2+), K(+), and Na(+) in roots of intact, sa...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Cramer GR,Lynch J,Läuchli A,Epstein E

    更新日期:1987-03-01 00:00:00

  • Amide-Linked Indoleacetic Acid Conjugates May Control Levels of Indoleacetic Acid in Germinating Seedlings of Phaseolus vulgaris.

    abstract::We have shown that amide-linked IAA (indole-3-acetic acid) conjugates accumulated to high levels during maturation of bean seeds (K. Bialek and J.D. Cohen [1989] Plant Physiol 91: 775-779). In the present study, we were interested in the fate of these and other IAA conjugates during seed germination. The content of am...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Bialek K,Cohen JD

    更新日期:1992-12-01 00:00:00

  • A Role for the F-Box Protein HAWAIIAN SKIRT in Plant microRNA Function.

    abstract::As regulators of gene expression in multicellular organisms, microRNAs (miRNAs) are crucial for growth and development. Although a plethora of factors involved in their biogenesis and action in Arabidopsis (Arabidopsis thaliana) has been described, these processes and their fine-tuning are not fully understood. Here, ...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Lang PLM,Christie MD,Dogan ES,Schwab R,Hagmann J,van de Weyer AL,Scacchi E,Weigel D

    更新日期:2018-01-01 00:00:00

  • Mode of action of gibberellic Acid and light on lettuce seed germination.

    abstract::The seeds of lettuce (Lactuca sativa L. cv. Grand Rapids) germinate in darkness at 25 C when treated by gibberellic acid (GA(3)) for 1 hour following 2 hours of imbibition. The time of GA(3) application influences the rate and the final percentage of seeds that germinate. In contrast, red light illumination given at d...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Lewak S,Khan AA

    更新日期:1977-10-01 00:00:00

  • Light quality and osmoregulation in vicia guard cells : evidence for involvement of three metabolic pathways.

    abstract::Osmoregulation in opening stomata of epidermal peels from Vicia faba L. leaves was investigated under a variety of experimental conditions. The K(+) content of stomatal guard cells and the starch content of guard cell chloroplasts were examined with cobaltinitrite and iodine-potassium iodide stains, respectively; stom...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Tallman G,Zeiger E

    更新日期:1988-11-01 00:00:00

  • Stable transformation of ferns using spores as targets: Pteris vittata and Ceratopteris thalictroides.

    abstract::Ferns (Pteridophyta) are very important members of the plant kingdom that lag behind other taxa with regards to our understanding of their genetics, genomics, and molecular biology. We report here, to our knowledge, the first instance of stable transformation of fern with recovery of transgenic sporophytes. Spores of ...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Muthukumar B,Joyce BL,Elless MP,Stewart CN Jr

    更新日期:2013-10-01 00:00:00

  • The interplay of light and the circadian clock. Independent dual regulation of clock-controlled gene ccg-2(eas).

    abstract::Ambient light is the major agent mediating entrainment of circadian rhythms and is also a major factor influencing development and morphogenesis. We show that in Neurospora crassa the expression of clock-controlled gene 2 (ccg-2), a gene under the control of the circadian clock and allelic to the developmental gene ea...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Arpaia G,Loros JJ,Dunlap JC,Morelli G,Macino G

    更新日期:1993-08-01 00:00:00

  • Nitrogen recycling and remobilization are differentially controlled by leaf senescence and development stage in Arabidopsis under low nitrogen nutrition.

    abstract::Five recombinant inbred lines (RILs) of Arabidopsis (Arabidopsis thaliana), previously selected from the Bay-0 x Shahdara RIL population on the basis of differential leaf senescence phenotypes (from early senescing to late senescing) when cultivated under nitrogen (N)-limiting conditions, were analyzed to monitor meta...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Diaz C,Lemaître T,Christ A,Azzopardi M,Kato Y,Sato F,Morot-Gaudry JF,Le Dily F,Masclaux-Daubresse C

    更新日期:2008-07-01 00:00:00

  • Sulfur transfer through an arbuscular mycorrhiza.

    abstract::Despite the importance of sulfur (S) for plant nutrition, the role of the arbuscular mycorrhizal (AM) symbiosis in S uptake has received little attention. To address this issue, 35S-labeling experiments were performed on mycorrhizas of transformed carrot (Daucus carota) roots and Glomus intraradices grown monoxenicall...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Allen JW,Shachar-Hill Y

    更新日期:2009-01-01 00:00:00

  • Bifunctional protein in carrot contains both aspartokinase and homoserine dehydrogenase activities.

    abstract::We have purified homoserine dehydrogenase to homogeneity and subjected polypeptide fragments derived from digests of the protein to amino acid sequencing. The amino acid sequence of homoserine dehydrogenase from carrot (Daucus carota) indicates that in carrot both aspartokinase and homoserine dehydrogenase activities ...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Wilson BJ,Gray AC,Matthews BF

    更新日期:1991-12-01 00:00:00

  • Studies on Cation-induced Thylakoid Membrane Stacking, Fluorescence Yield, and Photochemical Efficiency.

    abstract::Trypsin digestion of photosynthetic membranes isolated from spinach (Spinacia oleracea L.) leaves eliminates the cation stimulation of chlorophyll fluorescence. High concentrations of cations protect the fluorescence yield against trypsin digestion, and the cation specificity for this protection closely resembles that...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Jennings RC,Forti G,Gerola PD,Garlaschi FM

    更新日期:1978-12-01 00:00:00

  • Induction and Accumulation of Heat Shock-Specific Poly(A) RNAs and Proteins in Soybean Seedlings during Arsenite and Cadmium Treatments.

    abstract::Northern blot hybridization analyzes revealed that poly(A(+)) RNAs homologous to eight heat shock (HS)-specific cDNA clones were induced by arsenite (As) or Cd treatments. The mRNAs accumulated slower, and maximum accumulations were consistently lower than HS-induced levels. Prolonged treatment with low concentrations...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Edelman L,Czarnecka E,Key JL

    更新日期:1988-04-01 00:00:00

  • Measurement of proton-linked transport activities in pyranine loaded chloroplast inner envelope vesicles.

    abstract::A method has been devised for loading chloroplast inner envelope vesicles prepared from pea (Pisum sativum L. var Progress No. 9) or spinach (Spinacia oleracea L.) with 8-hydroxypyrene-1,3,6-trisulfonate (pyranine), a membrane impermeant, fluorescent pH indicator. Two known proton-linked transport activities of the in...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Howitz KT,McCarty RE

    更新日期:1988-04-01 00:00:00

  • Oxygen Concentration in Isolated Chloroplasts during Photosynthesis.

    abstract::The O(2) concentration in intact and osmotically disrupted isolated spinach (Spinacia oleracea, L.) chloroplasts during photosynthesis was estimated. The chloroplasts were allowed to reduce 3-phosphoglycerate, CO(2), or ferricyanide in light until the rate of O(2) production was linear. When the light was turned off O...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Steiger HM,Beck E

    更新日期:1977-12-01 00:00:00

  • Characterization of two isoenzymes of lipoxygenase from bush beans.

    abstract::Two isoenzymes of lipoxygenase, a and b, have been obtained from bush beans (Phaseolus vulgaris) as electrophoretically homogeneous proteins. Both proteins have a molecular weight of 100,000, contain 1 atom of iron, and appear to be composed of a single peptide chain. However, these enzymes appear to differ in some ot...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Hurt GB,Axelrod B

    更新日期:1977-04-01 00:00:00