Chlorophyll synthesis in dark-grown pine primary needles

Abstract:

:The pigment content of dark-grown primary needles of Pinus jeffreyi L. and Pinus sylvestris L. was determined by high-performance liquid chromatography. The state of protochlorophyllide a and of chlorophylls during dark growth were analyzed by in situ 77 K fluorescence spectroscopy. Both measurements unambiguously demonstrated that pine primary needles are able to synthesize chlorophyll in the dark. Norflurazon strongly inhibited both carotenoid and chlorophyll synthesis. Needles of plants treated with this inhibitor had low chlorophyll content, contained only traces of xanthophylls, and accumulated carotenoid precursors. The first form of chlorophyll detected in young pine needles grown in darkness had an emission maximum at 678 nm. Chlorophyll-protein complexes with in situ spectroscopic properties similar to those of fully green needles (685, 695, and 735 nm) later accumulated in untreated plants, whereas in norflurazon-treated plants the photosystem I emission at 735 nm was completely lacking. To better characterize the light-dependent chlorophyll biosynthetic pathway in pine needles, the 77 K fluorescence properties of in situ protochlorophyllide a spectral forms were studied. Photoactive and nonphotoactive protochlorophyllide a forms with emission properties similar to those reported for dark-grown angiosperms were found, but excitation spectra were substantially red shifted. Because of their lower chlorophyll content, norflurazon-treated plants were used to study the protochlorophyllide a photoreduction process triggered by one light flash. The first stable chlorophyllide photoproduct was a chlorophyllide a form emitting at 688 nm as in angiosperms. Further chlorophyllide a shifts usually observed in angiosperms were not detected. The rapid regeneration of photoactive protochlorophyllide a from nonphotoactive protochlorophyllide after one flash was demonstrated.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Schoefs B,Franck F

doi

10.1104/pp.118.4.1159

subject

Has Abstract

pub_date

1998-12-01 00:00:00

pages

1159-68

issue

4

eissn

0032-0889

issn

1532-2548

journal_volume

118

pub_type

杂志文章
  • Lipid Peroxide-Derived Short-Chain Carbonyls Mediate Hydrogen Peroxide-Induced and Salt-Induced Programmed Cell Death in Plants.

    abstract::Lipid peroxide-derived toxic carbonyl compounds (oxylipin carbonyls), produced downstream of reactive oxygen species (ROS), were recently revealed to mediate abiotic stress-induced damage of plants. Here, we investigated how oxylipin carbonyls cause cell death. When tobacco (Nicotiana tabacum) Bright Yellow-2 (BY-2) c...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.115.256834

    authors: Biswas MS,Mano J

    更新日期:2015-07-01 00:00:00

  • Ammonia Fixation via Glutamine Synthetase and Glutamate Synthase in the CAM Plant Cissus quadrangularis L.

    abstract::Succulent stems of Cissus quadrangularis L. (Vitaceae) contain glutamine synthetase, glutamate synthase, and glutamate dehydrogenase. The CO(2) and water gas exchanges of detached internodes were typical for Crassulacean acid metabolism plants. During three physiological phases, e.g. in the dark, in the early illumina...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.81.2.356

    authors: Berger MG,Sprengart ML,Kusnan M,Fock HP

    更新日期:1986-06-01 00:00:00

  • Transmission of the Monocarpic Senescence Signal via the Xylem in Soybean.

    abstract::During monocarpic senescence in soybean (Glycine max [L.] Merrill cv. Anoka) there is a remobilization of nitrogen from the leaves to the seeds, and it has been hypothesized that this loss of nitrogen from the leaves induces foliar yellowing. The phloem in a small segment of the petiole between the pods and the target...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.69.4.754

    authors: Noodén LD,Murray BJ

    更新日期:1982-04-01 00:00:00

  • Phytoalexin Elicitor Activity of Carbohydrates from Phytophthora megasperma f.sp. glycinea and Other Sources.

    abstract::Three unique classes of carbohydrates were isolated from the hyphal cell walls of Phytophthora megasperma f.sp. glycinea (Pmg) and compared with other substances for their activity as elicitors of the phytoalexin glyceollin in soybean tissues. Glucomannans extracted from cell walls with soybean beta-1,3-endoglucanase ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.71.3.466

    authors: Keen NT,Yoshikawa M,Wang MC

    更新日期:1983-03-01 00:00:00

  • Biosynthesis of cytidine diphosphate diglyceride by enzyme preparations from cauliflower.

    abstract::The activity of the enzyme responsible for the synthesis of cytidine diphosphate diglyceride has been found in the 20,000g pellet of a cauliflower (Brassica oleracea) inflorescence homogenate. Greater enzyme activity was found in the pellet after treatment with 0.02% Triton X-100, or after freezing and thawing. Activi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.45.6.719

    authors: Sumida S,Mudd JB

    更新日期:1970-06-01 00:00:00

  • Free amino Acid composition of leaf exudates and Phloem sap : a comparative study in oats and barley.

    abstract::Comparisons were made between the free amino acid composition in leaf exudates and that in pure phloem sap, using twin samples taken from a single leaf of two oat (Avena sativa L.) and three barley (Hordeum vulgare L.) varieties. Leaf exudate was collected in a 5 mm EDTA-solution (pH 7.0) from cut leaf blades and phlo...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.92.1.222

    authors: Weibull J,Ronquist F,Brishammar S

    更新日期:1990-01-01 00:00:00

  • Overexpression of a homeobox gene, LeT6, reveals indeterminate features in the tomato compound leaf

    abstract::The cultivated tomato (Lycopersicon esculentum) has a unipinnate compound leaf. In the developing leaf primordium, major leaflet initiation is basipetal, and lobe formation and early vascular differentiation are acropetal. We show that engineered alterations in the expression of a tomato homeobox gene, LeT6, can cause...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.117.3.771

    authors: Janssen BJ,Lund L,Sinha N

    更新日期:1998-07-01 00:00:00

  • Reproductive Growth and Dry Matter Production of Glycine max (L.) Merr. in Response to Oxygen Concentration.

    abstract::Reproductive as well as vegetative parameters of mature soybean (Glycine max [L.] Merr. cv. Wye) plants grown in chambers in which the aerial portion was exposed to altered pO(2) during all or part of the growth cycle were measured. Oxygen concentration was found to be a key factor controlling all phases of reproducti...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.55.1.102

    authors: Quebedeaux B,Hardy RW

    更新日期:1975-01-01 00:00:00

  • Further studies of the heat transfer from a leaf.

    abstract::The resistance to the diffusion of heat and water vapor external to a leaf, can be derived from measurement of the rate of change of the leaf temperature, after a sudden alteration of the intensity of irradiation. The theory of the method has been developed to accommodate the case of a leaf that is freely transpiring,...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.42.5.651

    authors: Linacre ET

    更新日期:1967-05-01 00:00:00

  • A cyclic nucleotide-gated channel (CNGC16) in pollen is critical for stress tolerance in pollen reproductive development.

    abstract::Cyclic nucleotide-gated channels (CNGCs) have been implicated in diverse aspects of plant growth and development, including responses to biotic and abiotic stress, as well as pollen tube growth and fertility. Here, genetic evidence identifies CNGC16 in Arabidopsis (Arabidopsis thaliana) as critical for pollen fertilit...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.206888

    authors: Tunc-Ozdemir M,Tang C,Ishka MR,Brown E,Groves NR,Myers CT,Rato C,Poulsen LR,McDowell S,Miller G,Mittler R,Harper JF

    更新日期:2013-02-01 00:00:00

  • A cytosolic Arabidopsis D-xylulose kinase catalyzes the phosphorylation of 1-deoxy-D-xylulose into a precursor of the plastidial isoprenoid pathway.

    abstract::Plants are able to integrate exogenous 1-deoxy-D-xylulose (DX) into the 2C-methyl-D-erythritol 4-phosphate pathway, implicated in the biosynthesis of plastidial isoprenoids. Thus, the carbohydrate needs to be phosphorylated into 1-deoxy-D-xylulose 5-phosphate and translocated into plastids, or vice versa. An enzyme ca...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.086652

    authors: Hemmerlin A,Tritsch D,Hartmann M,Pacaud K,Hoeffler JF,van Dorsselaer A,Rohmer M,Bach TJ

    更新日期:2006-10-01 00:00:00

  • Effect of aminoethoxy analog of rhizobitoxine on ripening of pears.

    abstract::Ripening reactions in pears (Pyrus communis L.) were differentially affected by an aminoethoxy analog of rhizobitoxine (l-2-amino-4-[2-aminoethoxy]-trans-3-butenoic acid) (AAR). Ethylene production of both ;Anjou' and ;Bartlett' pears was inhibited by AAR. Decrease in firmness, increase in protein N and soluble pectin...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.59.4.546

    authors: Wang CY,Mellenthin WM

    更新日期:1977-04-01 00:00:00

  • Chloroplast 2010: a database for large-scale phenotypic screening of Arabidopsis mutants.

    abstract::Large-scale phenotypic screening presents challenges and opportunities not encountered in typical forward or reverse genetics projects. We describe a modular database and laboratory information management system that was implemented in support of the Chloroplast 2010 Project, an Arabidopsis (Arabidopsis thaliana) reve...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.170118

    authors: Lu Y,Savage LJ,Larson MD,Wilkerson CG,Last RL

    更新日期:2011-04-01 00:00:00

  • Starch degradation in the cotyledons of germinating lentils.

    abstract::Starch, total amylolytic and phosphorylase activities were determined in lentil cotyledons during the first days of germination. Several independent criteria show that the amylolytic activity is due mainly to an amylase of the alpha type. Starch is degraded slowly in the first days; during this time, alpha- and beta-a...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.58.5.618

    authors: Tárrago JF,Nicolás G

    更新日期:1976-11-01 00:00:00

  • Purification and partial characterization of a membrane-associated lipoxygenase in tomato fruit.

    abstract::Membrane-associated lipoxygenase from green tomato (Lycopersicon esculentum L. cv Caruso) fruit has been purified 49-fold to a specific activity of 8.3 mumol.min(-1).mg(-1) of protein by solubilization of microsomal membranes with Triton X-100, followed by anion- exchange and size-exclusion chromatography. The apparen...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.100.4.1802

    authors: Bowsher CG,Ferrie BJ,Ghosh S,Todd J,Thompson JE,Rothstein SJ

    更新日期:1992-12-01 00:00:00

  • CO(2) Metabolism in Corn Roots. III. Inhibition of P-enolpyruvate Carboxylase by l-malate.

    abstract::Phosphoenolpyruvate carboxylase was purified from corn root tips about 80-fold by centrifugation, ammonium sulfate fractionation, and anion exchange and gel filtration chromatography. The resulting preparation was essentially free from malate dehydrogenase, isocitrate dehydrogenase, malate enzyme, NADH oxidase, and py...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.43.12.1919

    authors: Ting IP

    更新日期:1968-12-01 00:00:00

  • An investigation of the mineral content of barley grains and seedlings.

    abstract::The Ca, Mg, K, and P content of dry barley (Hordeum vulgare) grains and seedlings was investigated using energy dispersive x-ray analysis and neutron activation analysis. Energy dispersive x-ray analysis of protein bodies in aleurone cells showed that these bodies contained very little Ca in relation to P, Mg, and K. ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.86.1.93

    authors: Stewart A,Nield H,Lott JN

    更新日期:1988-01-01 00:00:00

  • Second positive phototropism results from coordinated co-action of the phototropins and cryptochromes.

    abstract::Phototropism and hypocotyl growth inhibition are modulated by the coaction of different blue-light photoreceptors and their signaling pathways. How seedlings integrate the activities of the different blue-light photoreceptors to coordinate these hypocotyl growth responses is still unclear. We have used time-lapse imag...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.102.018481

    authors: Whippo CW,Hangarter RP

    更新日期:2003-07-01 00:00:00

  • Purification and characterization of 1-aminocyclopropane-1-carboxylate synthase from apple fruits.

    abstract::1-Aminocyclopropane-1-carboxylate (ACC) synthase, a key enzyme in ethylene biosynthesis, was isolated and partially purified from apple (Malus sylvestris Mill.) fruits. Unlike ACC synthase isolated from other sources, apple ACC synthase is associated with the pellet fraction and can be solubilized in active form with ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.95.1.251

    authors: Yip WK,Dong JG,Yang SF

    更新日期:1991-01-01 00:00:00

  • Genome-wide detection of condition-sensitive alternative splicing in Arabidopsis roots.

    abstract::Iron (Fe) deficiency is a world-wide nutritional disorder in both plants and humans, resulting from its restricted bioavailability for plants and, subsequently, low Fe concentration in edible plant parts. Plants have evolved sophisticated mechanisms to alleviate Fe deficiency, with the aim of recalibrating metabolic f...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.217778

    authors: Li W,Lin WD,Ray P,Lan P,Schmidt W

    更新日期:2013-07-01 00:00:00

  • Units of freezing of deep supercooled water in woody xylem.

    abstract::The low temperature exotherms (LTE) of 1-year-old twigs of Haralson apple (Malus pumila Mill.), shagbark hickory (Carya ovata [Mill.] K. Koch), green ash (Fraxinus pennsylvanica Marsh), honey locust (Gleditsia triacanthos L.), American chestnut (Castanea dentata [Marsh] Borkh.), and red oak (Quercus rubra L.) were det...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.66.1.40

    authors: Hong SG,Sucoff E

    更新日期:1980-07-01 00:00:00

  • Ploidy effects on anatomy and gas exchange of tall fescue leaves.

    abstract::A growth chamber study was designed to interpret differences in CO(2) exchange rate (CER) and leaf diffusive resistance among 4X, 6X, 8X, and 10X ploidy levels of tall fescue (Festuca arundinacea, Schreb). Mesophyll cell size, stomatal density, number of major and minor veins, and bundle cap size of leaf blades were e...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.68.4.891

    authors: Byrne MC,Nelson CJ,Randall DD

    更新日期:1981-10-01 00:00:00

  • Effect of low temperature and calcium on survival and membrane properties of isolated winter wheat cells.

    abstract::Isolated cells obtained by enzymic digestion of young primary leaves of cold-hardened, dark-grown Kharkov winter wheat (Triticum aestivum L.) were exposed to various low temperature stresses. The initial uptake of (86)Rb was generally decreased by increasing concentrations of Ca(2+), but after longer periods of incuba...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.78.3.484

    authors: Pomeroy MK,Andrews CJ

    更新日期:1985-07-01 00:00:00

  • C(6)-[benzene ring]-indole-3-acetic Acid: a new internal standard for quantitative mass spectral analysis of indole-3-acetic Acid in plants.

    abstract::Indole-3-acetic acid (IAA) labeled with (13)C in the six carbons of the benzene ring is described for use as an internal standard for quantitative mass spectral analysis of IAA by gas chromatography/selected ion monitoring. [(13)C(6)]IAA was compared to the available deuterium labeled compounds and shown to offer the ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.80.1.14

    authors: Cohen JD,Baldi BG,Slovin JP

    更新日期:1986-01-01 00:00:00

  • Regulation of alternative oxidase activity in six wild monocotyledonous species. An in vivo study at the whole root level.

    abstract::The activity of the alternative pathway is affected by a number of factors, including the level and reduction state of the alternative oxidase (AOX) protein, and the reduction state of the ubiquinone pool. To investigate the significance of these factors for the rate of alternative respiration in vivo, we studied root...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.126.1.376

    authors: Millenaar FF,Gonzàlez-Meler MA,Fiorani F,Welschen R,Ribas-Carbo M,Siedow JN,Wagner AM,Lambers H

    更新日期:2001-05-01 00:00:00

  • B-Box Containing Proteins BBX30 and BBX31, Acting Downstream of HY5, Negatively Regulate Photomorphogenesis in Arabidopsis.

    abstract::Light-mediated seedling development is coordinately controlled by a variety of key regulators. Here, we identified two B-box (BBX)-containing proteins, BBX30 and BBX31, as repressors of photomorphogenesis. ELONGATED HYPOCOTYL5, a central regulator of light signaling, directly binds to the G-box cis-element present in ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.18.01244

    authors: Heng Y,Lin F,Jiang Y,Ding M,Yan T,Lan H,Zhou H,Zhao X,Xu D,Deng XW

    更新日期:2019-05-01 00:00:00

  • Plasma Membrane Redox Enzyme Is Involved in the Synthesis of O2- and H2O2 by Phytophthora Elicitor-Stimulated Rose Cells.

    abstract::An elicitor prepared from the autoclaved cell walls of Phytophthora sp. induced O2- generation and H2O2 accumulation by cultured cells of Rosa damascena Mill. cv Gloire de Guilan. N,N-Diethyldithiocarbamate, a superoxide dismutase inhibitior, blocked H2O2 accumulation and caused a dramatic accumulation of O2- by elici...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.107.4.1241

    authors: Auh CK,Murphy TM

    更新日期:1995-04-01 00:00:00

  • Phase-specific circadian clock regulatory elements in Arabidopsis.

    abstract::We have defined a minimal Arabidopsis CATALASE 3 (CAT3) promoter sufficient to drive evening-specific circadian transcription of a LUCIFERASE reporter gene. Deletion analysis and site-directed mutagenesis reveal a circadian response element, the evening element (EE: AAAATATCT), that is necessary for evening-specific t...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.004929

    authors: Michael TP,McClung CR

    更新日期:2002-10-01 00:00:00

  • The sucrose transporter of celery. Identification and expression during salt stress.

    abstract::In celery (Apium graveolens L.), long-distance transport of reduced carbon occurs both in the form of sucrose (Suc) and mannitol. The presence of mannitol has been related to the resistance of celery to salt stress. To investigate the transport events occurring during salt stress, we have cloned the H(+)/Suc transport...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.122.4.1447

    authors: Noiraud N,Delrot S,Lemoine R

    更新日期:2000-04-01 00:00:00

  • Relationship between Respiration and Photosynthesis in Guard Cell and Mesophyll Cell Protoplasts of Commelina communis L.

    abstract::A mass spectrometric method combining (16)O/(18)O and (12)C/(13)C isotopes was used to quantify the unidirectional fluxes of O(2) and CO(2) during a dark to light transition for guard cell protoplasts and mesophyll cell protoplasts of Commelina communis L. In darkness, O(2) uptake and CO(2) evolution were similar on a...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.95.2.636

    authors: Gautier H,Vavasseur A,Gans P,Lascève G

    更新日期:1991-02-01 00:00:00