Light and the correlation of chloroplast development and coupling of phosphorylation to electron transport.

Abstract:

:Coupling of phosphorylation to electron transport was examined by measuring the photosynthetic control ratio for broken wheat plastids isolated from seedlings at different greening stages. The photosynthetic control ratio progressively increased during greening and tight coupling was noted after granal stacking and thylakoid elongation. ADP impaired nonphosphorylating (state 2) electron transport rates of plastids at extremely early stages of greening and interfered with photosynthetic control measurements. Partially developed plastids exhibited low nonphosphorylating electron flow rates but did not exhibit high phosphorylating or uncoupled electron transport rates to the same extent as nearly developed plastids. Prolamellar body dispersal, primary thylakoid production, and the development of photosynthetic control were stimulated equally by 48 minutes of low irradiance, in cycles of 2 minutes every 2 hours, or by 9 hours of continuous light of moderate irradiance. Wheat plastids that greened for 6 hours in continuous light of moderate intensity did not exhibit photosynthetic control or much differentiation beyond the etioplast stage. It is concluded that plastid differentiation and the development of photosynthetic control early in greening under continuous light were limited by developmental time (dark time) rather than by either light intensity or duration.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Duysen ME,Freeman TP,Zabrocki RD

doi

10.1104/pp.65.5.880

subject

Has Abstract

pub_date

1980-05-01 00:00:00

pages

880-3

issue

5

eissn

0032-0889

issn

1532-2548

journal_volume

65

pub_type

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