Phosphate-dependent Substrate Transport into Mitochondria: Oxidative Studies.

Abstract:

:The oxidation of malate and citrate by isolated plant mitochondria can be stimulated by the addition of inorganic phosphate. This stimulation (a) is not inhibited by oligomycin or uncouplers; (b) can not be duplicated by addition of adenine nucleotide; (c) is inhibited by 2-n-butylmalonate; and (d) is not evident in detergent-treated mitochondria. Phosphate was required to elicit uncoupler-stimulated respiration. It is concluded that these effects of phosphate are attributable to a stimulated rate of substrate penetration into the mitochondria, and do not involve the oxidative phosphorylation process.Disrupting the mitochondria with detergent removed the requirement for phosphate and showed that butylmalonate did not inhibit the respiratory enzymes.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Wiskich JT

doi

10.1104/pp.56.1.121

subject

Has Abstract

pub_date

1975-07-01 00:00:00

pages

121-5

issue

1

eissn

0032-0889

issn

1532-2548

journal_volume

56

pub_type

杂志文章
  • Selective and Reversible Inhibition of Active CO(2) Transport by Hydrogen Sulfide in a Cyanobacterium.

    abstract::The active transport of CO(2) in the cyanobacterium Synechococcus UTEX 625 was inhibited by H(2)S. Treatment of the cells with up to 150 micromolar H(2)S + HS(-) at pH 8.0 had little effect on Na(+)-dependent HCO(3) (-) transport or photosynthetic O(2) evolution, but CO(2) transport was inhibited by more than 90%. CO(...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.91.1.387

    authors: Espie GS,Miller AG,Canvin DT

    更新日期:1989-09-01 00:00:00

  • Analysis of Variability in the Amaranthus Bioassay for Cytokinins: Effects of Water Stress on Benzyladenine- and Fusicoccin-dependent Responses.

    abstract::The use of only the upper part of the hypocotyl and the cotyledons in the Amaranthus tricolor bioassay for cytokinins, instead of the whole seedling, was found to reduce the endogenous response and give a higher benzyladenine-dependent response. There is no marked difference in the uptake or metabolism of benzyladenin...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.63.2.269

    authors: Elliott DC

    更新日期:1979-02-01 00:00:00

  • Putrescine-induced wounding and its effects on membrane integrity and ion transport processes in roots of intact corn seedlings.

    abstract::Interactions between putrescine and membrane function were examined with the use of a recently developed microelectrode system that enables us simultaneously to quantify membrane potentials and net K(+) fluxes associated with individual cells at the root surface of an intact corn (Zea mays L.) seedling. In contrast to...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.90.3.988

    authors: Ditomaso JM,Shaff JE,Kochian LV

    更新日期:1989-07-01 00:00:00

  • Characteristics of Nitrate Reductase-inactivating Proteins Obtained from Corn Roots and Rice Cell Cultures.

    abstract::Nitrate reductase (NR)-inactivating proteins from corn roots (Wf-9 x 38-11) and rice cell suspension cultures were tested against a partially purified NR obtained from corn leaves (W64A x W182E). The corn protein was purified 921-fold and the rice protein, 1,660-fold using standard purification procedures. Approximate...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.65.1.141

    authors: Yamaya T,Oaks A,Boesel IL

    更新日期:1980-01-01 00:00:00

  • The effect of auxin on stress relaxation in isolated Avena coleoptiles.

    abstract::In order to characterize further the mechanical properties of coleoptile cell walls, stress relaxation measurements were made on methanol-boiled sections of Avena coleoptiles. Relaxation was measured both in mechanically conditioned specimens and in specimens which had not been previously extended. In both cases the r...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.47.6.812

    authors: Cleland R,Haughton PM

    更新日期:1971-06-01 00:00:00

  • Inhibition of ent-Kaurene Oxidation and Growth by alpha-Cyclopropyl-alpha-(p-methoxyphenyl)-5-pyrimidine Methyl Alcohol.

    abstract::Growth of Alaska peas (Pisum sativum) is inhibited more than 60% by alpha-cyclopropyl-alpha-(p-methoxyphenyl)-5-pyrimidine methyl alcohol (ancymidol) treatment. This growth inhibition can be reversed completely by gibberellic acid application. Cell-free enzyme preparations from pea shoot tips and wild cucumber (Marah ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.57.2.245

    authors: Coolbaugh RC,Hamilton R

    更新日期:1976-02-01 00:00:00

  • Foxtail Mosaic Virus-Induced Gene Silencing in Monocot Plants.

    abstract::Virus-induced gene silencing (VIGS) is a powerful technique to study gene function in plants. However, very few VIGS vectors are available for monocot plants. Here we report that Foxtail mosaic virus (FoMV) can be engineered as an effective VIGS system to induce efficient silencing of endogenous genes in monocot plant...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.00010

    authors: Liu N,Xie K,Jia Q,Zhao J,Chen T,Li H,Wei X,Diao X,Hong Y,Liu Y

    更新日期:2016-07-01 00:00:00

  • Plasma Membrane-Associated Receptor-like Kinases Relocalize to Plasmodesmata in Response to Osmotic Stress.

    abstract::Plasmodesmata act as key elements in intercellular communication, coordinating processes related to plant growth, development, and responses to environmental stresses. While many of the developmental, biotic, and abiotic signals are primarily perceived at the plasma membrane (PM) by receptor proteins, plasmodesmata al...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.19.00473

    authors: Grison MS,Kirk P,Brault ML,Wu XN,Schulze WX,Benitez-Alfonso Y,Immel F,Bayer EM

    更新日期:2019-09-01 00:00:00

  • Development of the Primary Photochemical Apparatus of Photosynthesis during Greening of Etiolated Bean Leaves.

    abstract::Seven-day-old dark-grown bean leaves were greened under continuous light. The amount of chlorophyll, the ratio of chlorophyll a to chlorophyll b, the O(2) evolving capacity and the primary photochemical activities of Photosystem I and Photosystem II were measured on the leaves after various times of greening. The prim...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.58.4.526

    authors: Baker NR,Butler WL

    更新日期:1976-10-01 00:00:00

  • Chloroplast and mitochondrial proteases in Arabidopsis. A proposed nomenclature.

    abstract::The identity and scope of chloroplast and mitochondrial proteases in higher plants has only started to become apparent in recent years. Biochemical and molecular studies suggested the existence of Clp, FtsH, and DegP proteases in chloroplasts, and a Lon protease in mitochondria, although currently the full extent of t...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.125.4.1912

    authors: Adam Z,Adamska I,Nakabayashi K,Ostersetzer O,Haussuhl K,Manuell A,Zheng B,Vallon O,Rodermel SR,Shinozaki K,Clarke AK

    更新日期:2001-04-01 00:00:00

  • Photosynthesis in Fescue : III. RATES OF ELECTRON TRANSPORT IN A POLYPLOID SERIES OF TALL FESCUE PLANTS.

    abstract::Photosystem I electron transport activity has been found to be considerably higher in a decaploid tall fescue (Festuca arundinacea Schreb.) genotype as compared to a common hexaploid genotype. The decaploid genotype also displayed a higher photosystem whole chain (Photosystem II plus Photosystem I uncoupled) activity,...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.68.5.1110

    authors: Krueger RW,Miles D

    更新日期:1981-11-01 00:00:00

  • Inositol polyphosphate 5-phosphatases 1 and 2 are required for regulating seedling growth.

    abstract::Signals can be perceived and amplified at the cell membrane by receptors coupled to the production of a variety of second messengers, including myoinositol 1,4,5-trisphosphate [Ins(1,4,5)P(3)]. The myoinositol polyphosphate 5-phosphatases (5PTases; EC 3.1.3.56) comprise a large protein family that hydrolyzes 5-phospha...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.089474

    authors: Gunesekera B,Torabinejad J,Robinson J,Gillaspy GE

    更新日期:2007-03-01 00:00:00

  • Comparative transcriptomics of Arabidopsis sperm cells.

    abstract::In flowering plants, the two sperm cells are embedded within the cytoplasm of the growing pollen tube and as such are passively transported to the embryo sac, wherein double fertilization occurs upon their release. Understanding the mechanisms and conditions by which male gametes mature and take part in fertilization ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.125229

    authors: Borges F,Gomes G,Gardner R,Moreno N,McCormick S,Feijó JA,Becker JD

    更新日期:2008-10-01 00:00:00

  • Biosynthesis of the Dihydrochalcone Sweetener Trilobatin Requires Phloretin Glycosyltransferase2.

    abstract::Epidemics of obesity and type 2 diabetes drive strong consumer interest in plant-based low-calorie sweeteners. Trilobatin is a sweetener found at high concentrations in the leaves of a range of crabapple (Malus) species, but not in domesticated apple (Malus × domestica) leaves, which contain trilobatin's bitter positi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.20.00807

    authors: Wang Y,Yauk YK,Zhao Q,Hamiaux C,Xiao Z,Gunaseelan K,Zhang L,Tomes S,López-Girona E,Cooney J,Li H,Chagné D,Ma F,Li P,Atkinson RG

    更新日期:2020-10-01 00:00:00

  • Structure and properties of an engineered transketolase from maize.

    abstract::The gene specifying plastid transketolase (TK) of maize (Zea mays) was cloned from a cDNA library by southern blotting using a heterologous probe from sorghum (Sorghum bicolor). A recombinant fusion protein comprising thioredoxin of Escherichia coli and mature TK of maize was expressed at a high level in E. coli and c...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.020982

    authors: Gerhardt S,Echt S,Busch M,Freigang J,Auerbach G,Bader G,Martin WF,Bacher A,Huber R,Fischer M

    更新日期:2003-08-01 00:00:00

  • Effect of temperature on photosynthesis and growth in marine Synechococcus spp.

    abstract::In this study, we develop a mechanistic understanding of how temperature affects growth and photosynthesis in 10 geographically and physiologically diverse strains of Synechococcus spp. We found that Synechococcus spp. are able to regulate photochemistry over a range of temperatures by using state transitions and alte...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.221937

    authors: Mackey KR,Paytan A,Caldeira K,Grossman AR,Moran D,McIlvin M,Saito MA

    更新日期:2013-10-01 00:00:00

  • Distribution and Activity of the Plasma Membrane H+-ATPase in Mimosa pudica L. in Relation to Ionic Fluxes and Leaf Movements.

    abstract::Plasma membrane H+-ATPase was immunolocalized in several cell types of the sensitive plant Mimosa pudica L., and transmembrane potentials were measured on cortical cells. In comparison with the nonspecialized cortical cells of the petiole or stem, the proton pump was highly expressed in motor cells. These immunologica...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.3.747

    authors: Fleurat-Lessard P,Bouche-Pillon S,Leloup C,Bonnemain JL

    更新日期:1997-03-01 00:00:00

  • Bacterial AvrRpt2-Like Cysteine Proteases Block Activation of the Arabidopsis Mitogen-Activated Protein Kinases, MPK4 and MPK11.

    abstract::To establish infection, pathogens deliver effectors into host cells to target immune signaling components, including elements of mitogen-activated protein kinase (MPK) cascades. The virulence function of AvrRpt2, one of the first identified Pseudomonas syringae effectors, involves cleavage of the plant defense regulat...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.00336

    authors: Eschen-Lippold L,Jiang X,Elmore JM,Mackey D,Shan L,Coaker G,Scheel D,Lee J

    更新日期:2016-07-01 00:00:00

  • Phosphorylation of cytokinin by adenosine kinase from wheat germ.

    abstract::Adenosine kinase was partially purified from wheat germ. This enzyme preparation, which was devoid of adenine phosphoribosyltransferase and nearly free of adenosine deaminase but contained adenylate kinase, rapidly phosphorylated adenosine and a cytokinin, N(6)-(delta(2)-isopentenyl)adenosine. Electrophoretic analysis...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.59.3.443

    authors: Chen CM,Eckert RL

    更新日期:1977-03-01 00:00:00

  • Calcium Levels Affect the Ability to Immunolocalize Calmodulin to Cortical Microtubules.

    abstract::Calcium affects the stability of cortical microtubules (MTs) in lysed protoplasts. This calmodulin (CaM)-mediated interaction may provide a mechanism that serves to integrate cellular behavior with MT function. To test the hypothesis that CaM associates with these MTs, monoclonal antibodies were produced against CaM, ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.2.543

    authors: Fisher DD,Cyr RJ

    更新日期:1993-10-01 00:00:00

  • Nickel: a micronutrient essential for higher plants.

    abstract::Nickel was established as an essential micronutrient for the growth of temperate cereal crops. Grain from barley (Hordeum vulgare L. cv ;Onda'; containing 40 to 80 nanograms of Ni per gram dry weight) grown in solution culture with negligible Ni concentrations (< 30 nanograms of Ni per liter) exhibited greatly reduced...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.85.3.801

    authors: Brown PH,Welch RM,Cary EE

    更新日期:1987-11-01 00:00:00

  • Physiological roles of the light, oxygen, or voltage domains of phototropin 1 and phototropin 2 in Arabidopsis.

    abstract::Phototropins (phot1 and phot2) are plant blue-light receptors that mediate phototropism, chloroplast movement, stomatal opening, rapid inhibition of growth of etiolated seedlings, and leaf expansion in Arabidopsis (Arabidopsis thaliana). Their N-terminal region contains two light, oxygen, or voltage (LOV) domains, whi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.089839

    authors: Cho HY,Tseng TS,Kaiserli E,Sullivan S,Christie JM,Briggs WR

    更新日期:2007-01-01 00:00:00

  • Gains and Losses of Cis-regulatory Elements Led to Divergence of the Arabidopsis APETALA1 and CAULIFLOWER Duplicate Genes in the Time, Space, and Level of Expression and Regulation of One Paralog by the Other.

    abstract::How genes change their expression patterns over time is still poorly understood. Here, by conducting expression, functional, bioinformatic, and evolutionary analyses, we demonstrate that the differences between the Arabidopsis (Arabidopsis thaliana) APETALA1 (AP1) and CAULIFLOWER (CAL) duplicate genes in the time, spa...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.00320

    authors: Ye L,Wang B,Zhang W,Shan H,Kong H

    更新日期:2016-06-01 00:00:00

  • Identification of a cis-regulatory element involved in phytochrome down-regulated expression of the pea small GTPase gene pra2.

    abstract::The pra2 gene encodes a pea (Pisum sativum) small GTPase belonging to the YPT/rab family, and its expression is down-regulated by light, mediated by phytochrome. We have isolated and characterized a genomic clone of this gene and constructed a fusion DNA of its 5'-upstream region in front of the gene for firefly lucif...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.120.2.491

    authors: Inaba T,Nagano Y,Sakakibara T,Sasaki Y

    更新日期:1999-06-01 00:00:00

  • Identification of Intracellular Carbonic Anhydrase in Chlamydomonas reinhardtii which Is Distinct from the Periplasmic Form of the Enzyme.

    abstract::A physiologically significant level of intracellular carbonic anhydrase has been identified in Chlamydomonas reinhardtii after lysis of the cell wall-less mutant, cw15, and two intracellular polypeptides have been identified which bind to anti-carbonic anhydrase antisera. The susceptibility of the intracellular activi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.89.3.904

    authors: Husic HD,Kitayama M,Togasaki RK,Moroney JV,Morris KL,Tolbert NE

    更新日期:1989-03-01 00:00:00

  • Growth of transplastomic cells expressing D-amino acid oxidase in chloroplasts is tolerant to D-alanine and inhibited by D-valine.

    abstract::Dual-conditional positive/negative selection markers are versatile genetic tools for manipulating genomes. Plastid genomes are relatively small and conserved DNA molecules that can be manipulated precisely by homologous recombination. High-yield expression of recombinant products and maternal inheritance of plastid-en...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.204107

    authors: Gisby MF,Mudd EA,Day A

    更新日期:2012-12-01 00:00:00

  • Optical coherence microscopy. A technology for rapid, in vivo, non-destructive visualization of plants and plant cells.

    abstract::We describe the development and utilization of a new imaging technology for plant biology, optical coherence microscopy (OCM), which allows true in vivo visualization of plants and plant cells. This novel technology allows the direct, in situ (e.g. plants in soil), three-dimensional visualization of cells and events i...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.123.1.3

    authors: Hettinger JW,de la Peña Mattozzi M,Myers WR,Williams ME,Reeves A,Parsons RL,Haskell RC,Petersen DC,Wang R,Medford JI

    更新日期:2000-05-01 00:00:00

  • Participation of mitochondrial metabolism in photorespiration. Reconstituted system of peroxisomes and mitochondria from spinach leaves

    abstract::In this study the interplay of mitochondria and peroxisomes in photorespiration was simulated in a reconstituted system of isolated mitochondria and peroxisomes from spinach (Spinacia oleracea L.) leaves. The mitochondria oxidizing glycine produced serine, which was reduced in the peroxisomes to glycerate. The require...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.116.4.1333

    authors: Raghavendra AS,Reumann S,Heldt HW

    更新日期:1998-04-01 00:00:00

  • Predicting Stomatal Closure and Turgor Loss in Woody Plants Using Predawn and Midday Water Potential.

    abstract::Knowledge about physiological stress thresholds provides crucial information about plant performance and survival under drought. In this study, we report on the triphasic nature of the relationship between plant water potential (Ψ) at predawn and midday and describe a method that predicts Ψ at stomatal closure and tur...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.20.00500

    authors: Knipfer T,Bambach N,Hernandez MI,Bartlett MK,Sinclair G,Duong F,Kluepfel DA,McElrone AJ

    更新日期:2020-10-01 00:00:00

  • Chorismate Mutase Isoenzymes from Selected Plants and Their Immunological Comparison with the Isoenzymes from Sorghum bicolor.

    abstract::The isoenzyme pattern of chorismate mutase (EC 5.4.99.5) was examined by diethylaminoethyl-cellulose chromatography in a wide variety of plants. All plants contained a regulated form of chorismate mutase (CM-1), and most contained an additional, unregulated form (CM-2). The regulatory properties of CM-1 differed signi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.81.3.717

    authors: Singh BK,Lonergan SG,Conn EE

    更新日期:1986-07-01 00:00:00