Identification of a cis-regulatory element involved in phytochrome down-regulated expression of the pea small GTPase gene pra2.

Abstract:

:The pra2 gene encodes a pea (Pisum sativum) small GTPase belonging to the YPT/rab family, and its expression is down-regulated by light, mediated by phytochrome. We have isolated and characterized a genomic clone of this gene and constructed a fusion DNA of its 5'-upstream region in front of the gene for firefly luciferase. Using this construct in a transient assay, we determined a pra2 cis-regulatory region sufficient to direct the light down-regulation of the luciferase reporter gene. Both 5'- and internal deletion analyses revealed that the 93-bp sequence between -734 and -642 from the transcriptional start site was important for phytochrome down-regulation. Gain-of-function analysis showed that this 93-bp region could confer light down-regulation when fused to the cauliflower mosaic virus 35S promoter. Furthermore, linker-scanning analysis showed that a 12-bp sequence within the 93-bp region mediated phytochrome down-regulation. Gel-retardation analysis showed the presence of a nuclear factor that was specifically bound to the 12-bp sequence in vitro. These results indicate that this element is a cis-regulatory element involved in phytochrome down-regulated expression.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Inaba T,Nagano Y,Sakakibara T,Sasaki Y

doi

10.1104/pp.120.2.491

subject

Has Abstract

pub_date

1999-06-01 00:00:00

pages

491-500

issue

2

eissn

0032-0889

issn

1532-2548

journal_volume

120

pub_type

杂志文章
  • Effect of aminoethoxy analog of rhizobitoxine on ripening of pears.

    abstract::Ripening reactions in pears (Pyrus communis L.) were differentially affected by an aminoethoxy analog of rhizobitoxine (l-2-amino-4-[2-aminoethoxy]-trans-3-butenoic acid) (AAR). Ethylene production of both ;Anjou' and ;Bartlett' pears was inhibited by AAR. Decrease in firmness, increase in protein N and soluble pectin...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.59.4.546

    authors: Wang CY,Mellenthin WM

    更新日期:1977-04-01 00:00:00

  • Arabidopsis RING E3 ligase XBAT32 regulates lateral root production through its role in ethylene biosynthesis.

    abstract::XBAT32, a member of the RING domain-containing ankyrin repeat subfamily of E3 ligases, was previously identified as a positive regulator of lateral root development. Arabidopsis (Arabidopsis thaliana) plants harboring a mutation in XBAT32 produce fewer lateral roots that wild-type plants. We found that xbat32 mutants ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.156976

    authors: Prasad ME,Schofield A,Lyzenga W,Liu H,Stone SL

    更新日期:2010-08-01 00:00:00

  • New Arabidopsis advanced intercross recombinant inbred lines reveal female control of nonrandom mating.

    abstract::Female control of nonrandom mating has never been genetically established, despite being linked to inbreeding depression and sexual selection. In order to map the loci that control female-mediated nonrandom mating, we constructed a new advanced intercross recombinant inbred line (RIL) population derived from a cross b...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.233213

    authors: Fitz Gerald JN,Carlson AL,Smith E,Maloof JN,Weigel D,Chory J,Borevitz JO,Swanson RJ

    更新日期:2014-05-01 00:00:00

  • The Translesion Polymerase ζ Has Roles Dependent on and Independent of the Nuclease MUS81 and the Helicase RECQ4A in DNA Damage Repair in Arabidopsis.

    abstract::DNA polymerase zeta catalytic subunit REV3 is known to play an important role in the repair of DNA damage induced by cross-linking and methylating agents. Here, we demonstrate that in Arabidopsis (Arabidopsis thaliana), the basic polymerase activity of REV3 is essential for resistance protection against these differen...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.15.00806

    authors: Kobbe S,Trapp O,Knoll A,Manuss A,Puchta H

    更新日期:2015-12-01 00:00:00

  • Further Characterization of Expression of Auxin-Induced Genes in Tobacco (Nicotiana tabacum) Cell-Suspension Cultures.

    abstract::We have described the modulation of four auxin-regulated genes during the growth cycle of suspension-cultured tobacco (Nicotiana tabacum [L.] var White Burley) cells. The genes were transiently expressed 2 to 8 h after transfer of stationary phase cells to fresh medium, during the transition from the quiescent phase o...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.102.2.513

    authors: Boot K,Van Der Zaal BJ,Velterop J,Quint A,Mennes AM,Hooykaas P,Libbenga KR

    更新日期:1993-06-01 00:00:00

  • Light-dependent modifications in the metabolic responses of squash seedlings.

    abstract::Light-induced modifications in lipoxidase metabolism and chlorophyll formation in the cotyledon of squash (Cucurbita moscata) seedlings were determined. The enzyme activity decreased as light intensity increased, but chlorophyll continued to accumulate long after lipoxidase activity had virtually disappeared. Consider...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.41.5.780

    authors: Surrey K,Barr EM

    更新日期:1966-05-01 00:00:00

  • Complex I impairment, respiratory compensations, and photosynthetic decrease in nuclear and mitochondrial male sterile mutants of Nicotiana sylvestris.

    abstract::We have previously shown that in Nicotiana sylvestris cytoplasmic male-sterile (CMS) mutants where the mtDNA lacks the nad7 gene coding for a subunit of respiratory Complex I (NADH:ubiquinone oxidoreductase, EC 1.6.5.3), glycine (Gly) oxidation was lower than in the wild type and insensitive to rotenone, suggesting Co...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.124.3.1239

    authors: Sabar M,De Paepe R,de Kouchkovsky Y

    更新日期:2000-11-01 00:00:00

  • Oxygen Uptake and Photosynthesis of the Red Macroalga, Chondrus crispus, in Seawater: Effects of Oxygen Concentration.

    abstract::With an experimental system developed for aquatic plants using the mass spectrometry technique and infrared gas analysis of CO(2), we studied the responses to various O(2) concentrations of gas exchanges with the red macroalga Chondrus crispus S. The results were as follows. (a) Irrespective of the CO(2) concentration...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.78.3.545

    authors: Brechignac F,Andre M

    更新日期:1985-07-01 00:00:00

  • Manipulation of in vivo sorbitol production alters boron uptake and transport in tobacco

    abstract::Recent evidence that some species can retranslocate boron as complexes with sugar alcohols in the phloem suggests a possible mechanism for enhancing boron efficiency. We investigated the relationship between sugar alcohol (sorbitol) content, boron uptake and distribution, and translocation of foliar-applied, isotopica...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.119.2.735

    authors: Bellaloui N,Brown PH,Dandekar AM

    更新日期:1999-02-01 00:00:00

  • Hormones and young leaves control development of cotyledonary buds in tomato seedlings.

    abstract::Hormonal and plant factors regulating the development of the inhibited cotyledonary buds of Lycopersicon esculentum Mill. cv. ;Fireball' seedlings were studied. Excision of the immature plumular leaves of 5- to 20- millimeter length significantly stimulated bud development after 2 to 4 days, but excision of leaves exc...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.62.2.276

    authors: Aung LH

    更新日期:1978-08-01 00:00:00

  • Regulation of apoplastic NH4+ concentration in leaves of oilseed rape

    abstract::Regulation of apoplastic NH4+ concentration in leaves of oilseed rape (Brassica napus L.) was studied using a vacuum-infiltration technique that allowed controlled manipulations of the apoplastic solution. In leaves infiltrated with NH4+-free solution, the apoplastic NH4+ concentration returned in less than 1.5 min to...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.118.4.1361

    authors: Nielsen KH,Schjoerring JK

    更新日期:1998-12-01 00:00:00

  • Synergistic biosynthesis of biphasic ethylene and reactive oxygen species in response to hemibiotrophic Phytophthora parasitica in tobacco plants.

    abstract::We observed the biphasic production of ethylene and reactive oxygen species (ROS) in susceptible tobacco (Nicotiana tabacum 'Wisconsin 38') plants after shoot inoculation with Phytophthora parasitica var nicotianae. The initial transient increase in ROS and ethylene at 1 and 3 h (phase I), respectively, was followed b...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.194654

    authors: Wi SJ,Ji NR,Park KY

    更新日期:2012-05-01 00:00:00

  • Stimulation of root elongation and curvature by calcium.

    abstract::Ca2+ has been proposed to mediate inhibition of root elongation. However, exogenous Ca2+ at 10 or 20 millimolar, applied directly to the root cap, significantly stimulated root elongation in pea (Pisum sativum L.) and corn (Zea mays L.) seedlings. Furthermore, Ca2+ at 1 to 20 millimolar, applied unilaterally to the ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.98.1.246

    authors: Takahashi H,Scott TK,Suge H

    更新日期:1992-01-01 00:00:00

  • Structure and Biosynthesis of Cuticular Lipids: Hydroxylation of Palmitic Acid and Decarboxylation of C(28), C(30), and C(32) Acids in Vicia faba Flowers.

    abstract::The structure and composition of the cutin monomers from the flower petals of Vicia faba were determined by hydrogenolysis (LiAlH(4)) or deuterolysis (LiAlD(4)) followed by thin layer chromatography and combined gas-liquid chromatography and mass spectrometry. The major components were 10, 16-dihydroxyhexadecanoic aci...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.54.5.670

    authors: Kolattukudy PE,Croteau R,Brown L

    更新日期:1974-11-01 00:00:00

  • A Light Harvesting Complex-Like Protein in Maintenance of Photosynthetic Components in Chlamydomonas.

    abstract::Using a genetic approach, we have identified and characterized a novel protein, named Msf1 (Maintenance factor for photosystem I), that is required for the maintenance of specific components of the photosynthetic apparatus in the green alga Chlamydomonas reinhardtii Msf1 belongs to the superfamily of light-harvesting ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.01465

    authors: Zhao L,Cheng D,Huang X,Chen M,Dall'Osto L,Xing J,Gao L,Li L,Wang Y,Bassi R,Peng L,Wang Y,Rochaix JD,Huang F

    更新日期:2017-08-01 00:00:00

  • Photosynthesis in the Higher Plant, Vicia faba: III. Serine, a Precursor of the Tricarboxylic Acid Cycle.

    abstract::Evidence is presented to support the hypothesis that serine, rather than 3-phosphoglycerate of the Calvin cycle, is a precursor of the tricarboxylic acid cycle during photosynthesis by the higher plant, Vicia faba. Identification of the serine intermediate is based upon a unique C(1) > C(2) > C(3) isotope distribution...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.53.3.491

    authors: Kent SS,Pinkerton FD,Strobel GA

    更新日期:1974-03-01 00:00:00

  • Isolation of Intact and Functional Chloroplasts from Mesophyll and Bundle Sheath Protoplasts of the C(4) Plant Panicum miliaceum.

    abstract::A procedure is described for isolating and purifying mesophyll protoplasts and bundle sheath protoplasts of the C(4) plant Panicum miliaceum. Following enzymic digestion of leaf tissue, mesophyll protoplasts and bundle sheath protoplasts are released and purified by density centrifugation. The lower density of mesophy...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.63.5.821

    authors: Edwards GE

    更新日期:1979-05-01 00:00:00

  • Differential Regulation of Maize Homoserine Dehydrogenase under Physiological Conditions.

    abstract::Homoserine dehydrogenase is associated with the multibranched pathway of amino acid biosynthesis originating with aspartic acid. Like most of the related pathway enzymes, this enzyme is localized in chloroplasts. The activity and regulatory properties of the threonine-sensitive isozyme of homoserine dehydrogenase isol...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.92.3.785

    authors: Bryan JK

    更新日期:1990-03-01 00:00:00

  • Increased 1-Aminocyclopropane-1-Carboxylic Acid Oxidase Activity in Shoots of Flooded Tomato Plants Raises Ethylene Production to Physiologically Active Levels.

    abstract::Soil flooding increased 1-aminocyclopropane-1-carboxylic (ACC) acid oxidase activity in petioles of wild-type tomato (Lycopersicon esculentum L.) plants within 6 to 12 h in association with faster rates of ethylene production. Petioles of flooded plants transformed with an antisense construct to one isoform of an ACC ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.4.1435

    authors: English PJ,Lycett GW,Roberts JA,Jackson MB

    更新日期:1995-12-01 00:00:00

  • Variation in amounts of pyruvate, orthophosphate dikinase, and some other enzymes of the c(4) pathway in some wheat species.

    abstract::First leaves and flag leaves of the wheat species Triticum aestivum cv Anza (6x), T. boeoticum Boiss (2x) L. were examined for content of pyruvate, orthophosphate dikinase (PPDK), phosphoenolpyruvate carboxylase (PEPC), and ribulose 1,5-bisphosphate carboxylase (RuBPC) by protein blot analyses using antibodies to maiz...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.82.1.96

    authors: Aoyagi K,Bassham JA

    更新日期:1986-09-01 00:00:00

  • The role of the plant-specific ALTERED XYLOGLUCAN9 protein in Arabidopsis cell wall polysaccharide O-acetylation.

    abstract::A mutation in the ALTERED XYLOGLUCAN9 (AXY9) gene was found to be causative for the decreased xyloglucan acetylation phenotype of the axy9.1 mutant, which was identified in a forward genetic screen for Arabidopsis (Arabidopsis thaliana) mutants. The axy9.1 mutant also exhibits decreased O-acetylation of xylan, implyin...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.114.256479

    authors: Schultink A,Naylor D,Dama M,Pauly M

    更新日期:2015-04-01 00:00:00

  • Chlorophyll synthesis in chlorella I. Occurrence of a lag phase on initiation of a dilute culture.

    abstract::A culture of Chlorella established by 30-fold dilution of a culture already grown to a level of 15 ml packed cell volume per liter produces little chlorophyll for approximately 12 hours. Investigation of other characteristics such as nitrogen incorporation, increase in packed cell volume and dry weight as well as RNA ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.41.10.1695

    authors: Shugarman PM,Appleman D

    更新日期:1966-12-01 00:00:00

  • Chemiluminescence in Wounded Root Tissue : EVIDENCE FOR PEROXIDASE INVOLVEMENT.

    abstract::Root and stem segments from soybean (Merrill cv. ;Bragg') showed an enhanced chemiluminescence upon mechanical injury. Roots emitted more light than did stems. Light emission was diminished by CN(-) and N(3) (-) but was not affected by rotenone and antimycin A. Catalase quenched chemiluminescence in wounded root segme...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.67.1.43

    authors: Salin ML,Bridges SM

    更新日期:1981-01-01 00:00:00

  • The structure of chloroplast DNA molecules and the effects of light on the amount of chloroplast DNA during development in Medicago truncatula.

    abstract::We used pulsed-field gel electrophoresis and restriction fragment mapping to analyze the structure of Medicago truncatula chloroplast DNA (cpDNA). We find most cpDNA in genome-sized linear molecules, head-to-tail genomic concatemers, and complex branched forms with ends at defined sites rather than at random sites as ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.107.112946

    authors: Shaver JM,Oldenburg DJ,Bendich AJ

    更新日期:2008-03-01 00:00:00

  • Studies of a novel cysteine sulfoxide lyase from Petiveria alliacea: the first heteromeric alliinase.

    abstract::A novel alliinase (EC 4.4.1.4) was detected and purified from the roots of the Amazonian medicinal plant Petiveria alliacea. The isolated enzyme is a heteropentameric glycoprotein composed of two alpha-subunits (68.1 kD each), one beta-subunit (56.0 kD), one gamma-subunit (24.8 kD), and one delta-subunit (13.9 kD). Th...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.142430

    authors: Musah RA,He Q,Kubec R,Jadhav A

    更新日期:2009-11-01 00:00:00

  • Studies on the presence of adenosine cyclic 3':5'-monophosphate in oat coleoptiles.

    abstract::The incorporation of adenosine-8-(14)C into adenosine cyclic 3':5'-monophosphate in coleoptile-first leaf segments of Avena sativa L. was investigated. Homogenates of segments incubated in adenosine-8-(14)C for either 4 or 10 hours were partially purified by thin layer chromatography followed by paper electrophoresis....

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.55.2.346

    authors: Ownby JD,Ross CW

    更新日期:1975-02-01 00:00:00

  • Biosynthesis of the Dihydrochalcone Sweetener Trilobatin Requires Phloretin Glycosyltransferase2.

    abstract::Epidemics of obesity and type 2 diabetes drive strong consumer interest in plant-based low-calorie sweeteners. Trilobatin is a sweetener found at high concentrations in the leaves of a range of crabapple (Malus) species, but not in domesticated apple (Malus × domestica) leaves, which contain trilobatin's bitter positi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.20.00807

    authors: Wang Y,Yauk YK,Zhao Q,Hamiaux C,Xiao Z,Gunaseelan K,Zhang L,Tomes S,López-Girona E,Cooney J,Li H,Chagné D,Ma F,Li P,Atkinson RG

    更新日期:2020-10-01 00:00:00

  • Proper levels of the Arabidopsis cohesion establishment factor CTF7 are essential for embryo and megagametophyte, but not endosperm, development.

    abstract::CTF7 is an essential gene in yeast that is required for the formation of sister chromatid cohesion. While recent studies have provided insights into how sister chromatid cohesion is established, less is known about how specifically CTF7 facilitates the formation of cohesion, and essentially nothing is known about how ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.157560

    authors: Jiang L,Yuan L,Xia M,Makaroff CA

    更新日期:2010-10-01 00:00:00

  • Characterization of two isoenzymes of lipoxygenase from bush beans.

    abstract::Two isoenzymes of lipoxygenase, a and b, have been obtained from bush beans (Phaseolus vulgaris) as electrophoretically homogeneous proteins. Both proteins have a molecular weight of 100,000, contain 1 atom of iron, and appear to be composed of a single peptide chain. However, these enzymes appear to differ in some ot...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.59.4.695

    authors: Hurt GB,Axelrod B

    更新日期:1977-04-01 00:00:00

  • Rapid Accumulation of Anionic Peroxidases and Phenolic Polymers in Soybean Cotyledon Tissues following Treatment with Phytophthora megasperma f. sp. Glycinea Wall Glucan.

    abstract::Phytophthora megasperma Drechs. f. sp. glycinea Kuan & Erwin (PMG) cell wall glucan has been extensively characterized as an elicitor of the pterocarpan phytoalexins, the glyceollins in soybean (Glycine max L.). Just recently, this glucan was shown to be a potent elicitor of conjugates of the isoflavones, daidzein and...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.97.4.1445

    authors: Graham MY,Graham TL

    更新日期:1991-12-01 00:00:00