Metal Tolerance Protein 8 Mediates Manganese Homeostasis and Iron Reallocation during Seed Development and Germination.

Abstract:

:Metal accumulation in seeds is a prerequisite for germination and establishment of plants but also for micronutrient delivery to humans. To investigate metal transport processes and their interactions in seeds, we focused on METAL TOLERANCE PROTEIN8 (MTP8), a tonoplast transporter of the manganese (Mn) subclade of cation diffusion facilitators, which in Arabidopsis (Arabidopsis thaliana) is expressed in embryos of seeds. The x-ray fluorescence imaging showed that expression of MTP8 was responsible for Mn localization in subepidermal cells on the abaxial side of the cotyledons and in cortical cells of the hypocotyl. Accordingly, under low Mn availability, MTP8 increased seed stores of Mn, required for efficient seed germination. In mutant embryos lacking expression of VACUOLAR IRON TRANSPORTER1 (VIT1), MTP8 built up iron (Fe) hotspots in MTP8-expressing cells types, suggesting that MTP8 transports Fe in addition to Mn. In mtp8 vit1 double mutant seeds, Mn and Fe were distributed in all cell types of the embryo. An Fe transport function of MTP8 was confirmed by its ability to complement Fe hypersensitivity of a yeast mutant defective in vacuolar Fe transport. Imbibing mtp8-1 mutant seeds in the presence of Mn or subjecting seeds to wet-dry cycles showed that MTP8 conferred Mn tolerance. During germination, MTP8 promoted reallocation of Fe from the vasculature. These results indicate that cell type-specific accumulation of Mn and Fe in seeds depends on MTP8 and that this transporter plays an important role in the generation of seed metal stores as well as for metal homeostasis and germination efficiency under challenging environmental conditions.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Eroglu S,Giehl RFH,Meier B,Takahashi M,Terada Y,Ignatyev K,Andresen E,Küpper H,Peiter E,von Wirén N

doi

10.1104/pp.16.01646

subject

Has Abstract

pub_date

2017-07-01 00:00:00

pages

1633-1647

issue

3

eissn

0032-0889

issn

1532-2548

pii

pp.16.01646

journal_volume

174

pub_type

杂志文章
  • The decisive step in betaxanthin biosynthesis is a spontaneous reaction1

    abstract::Experiments were performed to confirm that the aldimine bond formation is a spontaneous reaction, because attempts to find an enzyme catalyzing the last decisive step in betaxanthin biosynthesis, the aldimine formation, failed. Feeding different amino acids to betalain-forming hairy root cultures of yellow beet (Beta ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.119.4.1217

    authors: Schliemann W,Kobayashi N,Strack D

    更新日期:1999-04-01 00:00:00

  • Calcium-Dependent Protein Phosphorylation May Mediate the Gibberellic Acid Response in Barley Aleurone

    abstract::Peptide substrates of well-defined protein kinases were microinjected into aleurone protoplasts of barley (Hordeum vulgare L. cv Himalaya) to inhibit, and therefore identify, protein kinase-regulated events in the transduction of the gibberellin (GA) and abscisic acid signals. Syntide-2, a substrate designed for Ca2+-...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.116.2.765

    authors: Ritchie S,Gilroy S

    更新日期:1998-02-01 00:00:00

  • Metabolic engineering of the chloroplast genome using the Echerichia coli ubiC gene reveals that chorismate is a readily abundant plant precursor for p-hydroxybenzoic acid biosynthesis.

    abstract::p-Hydroxybenzoic acid (pHBA) is the major monomer in liquid crystal polymers. In this study, the Escherichia coli ubiC gene that codes for chorismate pyruvate-lyase (CPL) was integrated into the tobacco (Nicotiana tabacum) chloroplast genome under the control of the light-regulated psbA 5' untranslated region. CPL cat...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.050054

    authors: Viitanen PV,Devine AL,Khan MS,Deuel DL,Van Dyk DE,Daniell H

    更新日期:2004-12-01 00:00:00

  • Bicarbonate ion as a critical factor in photosynthetic oxygen evolution.

    abstract::Bicarbonate ion, not dissolved CO(2) gas, is shown to increase 4- to 5-fold the rate of dichlorophenol indophenol reduction by isolated maize (Zea mays) chloroplasts. Glutaraldehyde fixed chloroplasts continue to exhibit bicarbonate-dependent 2,6-dichlorophenol indophenol reduction. Bicarbonate is shown to act close t...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.52.2.119

    authors: Stemler A,Govindjee

    更新日期:1973-08-01 00:00:00

  • Characterization of a molecular modification of phytochrome that is associated with its conversion to the far-red-absorbing form.

    abstract::Phytochrome that has been photoinduced to pellet by irradiation of intact oat (cv. Garry) shoots and recovered from a pellet obtained by centrifugation of crude extracts exhibits modified behavior when compared to soluble phytochrome isolated from shoots that had never been irradiated. This modified behavior includes ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.68.4.789

    authors: Boeshore ML,Pratt LH

    更新日期:1981-10-01 00:00:00

  • Malate Decarboxylation by Kalanchoë daigremontiana Mitochondria and Its Role in Crassulacean Acid Metabolism.

    abstract::Mitochondria isolated from Kalanchoë daigremontiana, a Crassulacean acid metabolism plant, decarboxylate added malate to pyruvate at rates of up to 100 micromoles per hour per milligram original chlorophyll in the presence of ADP. Omitting ADP reduces this rate by approximately 50%. Antimycin A inhibits malate decarbo...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.65.4.675

    authors: Day DA

    更新日期:1980-04-01 00:00:00

  • Analysis of pea chloroplast inner and outer envelope membrane proteins by two-dimensional gel electrophoresis and their comparison with stromal proteins.

    abstract::Analysis of inner and outer pea (Pisum sativum var. Laxtons Progress No. 9) chloroplast envelope membranes by sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that, although the two membranes have distinct polypeptide compositions, there are several comigrating polypeptides in the two membrane fracti...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.73.3.569

    authors: Werner-Washburne M,Cline K,Keegstra K

    更新日期:1983-11-01 00:00:00

  • The effect of rotenone on respiration in pea cotyledon mitochondria.

    abstract::Respiration utilizing NAD-linked substrates in mitochondria isolated from cotyledons of etiolated peas (Pisum sativum L. var. Homesteader) by sucrose density gradient centrifugation exhibited resistance to rotenone. The inhibited rate of alpha-ketoglutarate oxidation was equivalent to the recovered rate of malate oxid...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.68.6.1211

    authors: Johnson-Flanagan AM,Spencer MS

    更新日期:1981-12-01 00:00:00

  • Multiple antibiotic resistance in Arabidopsis is conferred by mutations in a chloroplast-localized transport protein.

    abstract::Widespread antibiotic resistance is a major public health concern, and plants represent an emerging antibiotic exposure route. Recent studies indicate that crop plants fertilized with antibiotic-laden animal manure accumulate antibiotics; however, the molecular mechanisms of antibiotic entry and subcellular partitioni...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.143487

    authors: Conte S,Stevenson D,Furner I,Lloyd A

    更新日期:2009-10-01 00:00:00

  • Overlaps in the transcriptional profiles of Medicago truncatula roots inoculated with two different Glomus fungi provide insights into the genetic program activated during arbuscular mycorrhiza.

    abstract::Arbuscular mycorrhiza (AM) is a widespread symbiotic association between plants and fungal microsymbionts that supports plant development under nutrient-limiting and various stress conditions. In this study, we focused on the overlapping genetic program activated by two commonly studied microsymbionts in addition to i...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.056572

    authors: Hohnjec N,Vieweg MF,Pühler A,Becker A,Küster H

    更新日期:2005-04-01 00:00:00

  • Aminocyclopropane carboxylic acid synthase is a regulated step in ethylene-dependent induced conifer defense. Full-length cDNA cloning of a multigene family, differential constitutive, and wound- and insect-induced expression, and cellular and subcellular

    abstract::In conifer stems, formation of chemical defenses against insects or pathogens involves specialized anatomical structures of the phloem and xylem. Oleoresin terpenoids are formed in resin duct epithelial cells and phenolics accumulate in polyphenolic parenchyma cells. Ethylene signaling has been implicated in the induc...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.089425

    authors: Ralph SG,Hudgins JW,Jancsik S,Franceschi VR,Bohlmann J

    更新日期:2007-01-01 00:00:00

  • Dehydroascorbate influences the plant cell cycle through a glutathione-independent reduction mechanism.

    abstract::Glutathione is generally accepted as the principal electron donor for dehydroascorbate (DHA) reduction. Moreover, both glutathione and DHA affect cell cycle progression in plant cells. But other mechanisms for DHA reduction have been proposed. To investigate the connection between DHA and glutathione, we have evaluate...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.033548

    authors: Potters G,Horemans N,Bellone S,Caubergs RJ,Trost P,Guisez Y,Asard H

    更新日期:2004-04-01 00:00:00

  • Effects of water stress on the organic Acid and carbohydrate compositions of cotton plants.

    abstract::Two photoperiodic cotton (Gossypium hirsutum L.) strains (T185 and T466) which had been empirically selected because of poor performance and two strains (T25 and T256) selected because of enhanced performance under field water stress were evaluated for stress-induced changes in their organic acids and carbohydrates. P...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.82.3.724

    authors: Timpa JD,Burke JJ,Quisenberry JE,Wendt CW

    更新日期:1986-11-01 00:00:00

  • Rapidly Induced Wound Ethylene from Excised Segments of Etiolated Pisum sativum L., cv. Alaska: III. Induction and Transmission of the Response.

    abstract::Increased ethylene synthesis was rapidly induced throughout the apical meristematic region of etiolated seedlings of Pisum sativum L., cv. Alaska by cuts made 1 centimeter from the apical hook. The wound signal was transmitted at about 2 millimeters per minute. Accumulation of substance(s) at the cut surfaces of excis...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.62.5.710

    authors: Saltveit ME,Dilley DR

    更新日期:1978-11-01 00:00:00

  • Mechanism of photoactivation of electron transport in intact bryopsis chloroplasts.

    abstract::The mechanism of photoactivation of photosystem I electron transport was studied in intact Bryopsis corticulans chloroplasts. The evidence from chemical and photochemical studies suggests that photoactivation is a consequence of a reduction of an electron transport component, presumably ferredoxin-NADP(+) reductase. O...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.70.5.1413

    authors: Satoh K

    更新日期:1982-11-01 00:00:00

  • Gene duplication in the carotenoid biosynthetic pathway preceded evolution of the grasses.

    abstract::Despite ongoing research on carotenoid biosynthesis in model organisms, there is a paucity of information on pathway regulation operating in the grasses (Poaceae), which include plants of world-wide agronomic importance. As a result, efforts to either breed for or metabolically engineer improvements in carotenoid cont...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.039818

    authors: Gallagher CE,Matthews PD,Li F,Wurtzel ET

    更新日期:2004-07-01 00:00:00

  • ECHIDNA protein impacts on male fertility in Arabidopsis by mediating trans-Golgi network secretory trafficking during anther and pollen development.

    abstract::The trans-Golgi network (TGN) plays a central role in cellular secretion and has been implicated in sorting cargo destined for the plasma membrane. Previously, the Arabidopsis (Arabidopsis thaliana) echidna (ech) mutant was shown to exhibit a dwarf phenotype due to impaired cell expansion. However, ech also has a prev...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.227769

    authors: Fan X,Yang C,Klisch D,Ferguson A,Bhaellero RP,Niu X,Wilson ZA

    更新日期:2014-03-01 00:00:00

  • Physiological studies with isolated leaf cells: early products of photosynthesis and their metabolic interconversions.

    abstract::A number of plants have been surveyed with respect to isolation by mild grinding in large quantities of leaf cells. The extent of recovery of mesophyll cells per unit leaf area was found to vary with plant species and the method of grinding. Greater than 70% recovery was obtained from the leaves of Canna indica L., Cr...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.54.4.569

    authors: Kulandaivelu G,Gnanam A

    更新日期:1974-10-01 00:00:00

  • Effect of Inhibition of Glycosylation on the Appearance of a 60 kD Membrane Glycopolypeptide in Endomembrane Fractions of Soybean Root.

    abstract::Endomembrane (endoplasmic reticulum, Golgi apparatus, plasma membrane) proteins of soybean (Glycine max) root cells are highly glycosylated. We investigated whether N-linked oligosaccharide moieties are essential for the correct intracellular transport of plant endomembrane glycoproteins. Excised roots were incubated ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.89.1.299

    authors: Lafayette PR,Travis RL

    更新日期:1989-01-01 00:00:00

  • Purification and Properties of an NADPH-Aldose Reductase (Aldehyde Reductase) from Euonymus japonica Leaves.

    abstract::The enzyme aldose (aldehyde) reductase was partially purified (142-fold) and characterized from Euonymus japonica leaves. The reductase, a dimer, had an average molecular weight of 67,000 as determined by gel filtration on Sephadex G-100. The enzyme was NADPH specific and reduced a broad range of substrates including ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.80.4.972

    authors: Negm FB

    更新日期:1986-04-01 00:00:00

  • Sequencing, genomic organization, and preliminary promoter analysis of a black cherry (R)-(+)-mandelonitrile lyase gene.

    abstract::The flavoprotein (R)-(+)-mandelonitrile lyase (MDL; EC 4.1.2.10) plays a key role in cyanogenesis in rosaceous stone fruits. An MDL gene (mdl3) and its corresponding cDNA (MDL3) were isolated from black cherry (Prunus serotina) and characterized. The mdl3 gene contains 2292 bp of the 5' flanking region, the entire cod...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.115.4.1359

    authors: Hu Z,Poulton JE

    更新日期:1997-12-01 00:00:00

  • Maintenance of turgor by rapid sealing of puncture wounds in leaf epidermal cells.

    abstract::When leaf epidermal cells are puncture wounded with a glass microcapillary tip, a small droplet of fluid is discharged and then evaporates, leaving a solid residue on the cell surface. For puncture wounds of about 3.5 micrometers in diameter, this process is complete within 2 to 3 seconds. A second puncture wound also...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.97.3.907

    authors: Shackel KA,Polito VS,Ahmadi H

    更新日期:1991-11-01 00:00:00

  • Abscisic acid regulates axillary bud outgrowth responses to the ratio of red to far-red light.

    abstract::Low red light/far-red light ratio (R:FR) serves as an indicator of impending competition and has been demonstrated to suppress branch development. The regulation of Arabidopsis (Arabidopsis thaliana) rosette bud outgrowth by the R:FR and the associated mechanisms were investigated at several levels. Growth under low R...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.221895

    authors: Reddy SK,Holalu SV,Casal JJ,Finlayson SA

    更新日期:2013-10-01 00:00:00

  • Growth and N Allocation in Rice Plants under CO2 Enrichment.

    abstract::The effects of CO2 enrichment on growth and N allocation of rice (Oryza sativa L.) were examined. The plants were grown hydroponically in growth chambers with a 14-h photoperiod (1000 [mu]mol quanta m-2 s-1) and a day/night temperature of 25/20[deg]C. From the 28th to 70th d after germination, the plants were exposed ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.115.1.199

    authors: Makino A,Harada M,Sato T,Nakano H,Mae T

    更新日期:1997-09-01 00:00:00

  • Comparison of Triazine-Resistant and -Susceptible Biotypes of Senecio vulgaris and Their F(1) Hybrids.

    abstract::The relationship of triazine resistance to decreased plant productivity was investigated in Senecio vulgaris L. F(1) reciprocal hybrids were developed from pure-breeding susceptible (S) and resistant (R) lines. The four biotypes (S, S x R, R, R x S) were compared in terms of atrazine response, electron transport, carb...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.87.1.183

    authors: Stowe AE,Holt JS

    更新日期:1988-05-01 00:00:00

  • Alteration of soybean complex lipid biosynthesis by s-ethyl dipropylthiocarbamate.

    abstract::Soybean (Glycine max [L.] Merr. var. Glabrous D62-7812) plants were grown in aerated Hoagland and Arnon mineral nutrient solution containing 0 or 2.6 mum S-ethyl dipropylthiocarbamate (EPTC) in a growth chamber. After 19 days exposure to EPTC, total leaf fresh weight was reduced 18% by 2.6 mum EPTC while total leaf fa...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.60.1.86

    authors: Wilkinson RE,Smith AE

    更新日期:1977-07-01 00:00:00

  • Photorespiration in c(3) and c(4) plant tissue cultures: significance of kranz anatomy to low photorespiration in c(4) plants.

    abstract::Photorespiration rates in tissue cultures of a C(4) plant, Portulaca oleracea, were compared to those in tissue cultures of a C(3) plant, Streptanthus tortuosus. The C(4) plant tissue cultures have one-half to one-third the photorespiration rate of the C(3) plant tissue cultures and respond to varying O(2) concentrati...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.58.4.573

    authors: Kennedy RA

    更新日期:1976-10-01 00:00:00

  • Influence of chloroplast development on the activation of the diphenyl ether herbicide acifluorfen-methyl.

    abstract::The activity of acifluorfen-methyl (AFM); methyl 5-(2-chloro-4-[trifluoromethyl] phenoxy)-2-nitrobenzoate in excised cucumber cotyledons (Cucumis sativus L.) was examined. AFM induced membrane disruption, was significantly greater when etiolated cotyledons were illuminated 16 hours at 150 microeinsteins per square met...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.84.4.1114

    authors: Halling BP,Peters GR

    更新日期:1987-08-01 00:00:00

  • Climate and Developmental Plasticity: Interannual Variability in Grapevine Leaf Morphology.

    abstract::The shapes of leaves are dynamic, changing over evolutionary time between species, within a single plant producing different shaped leaves at successive nodes, during the development of a single leaf as it allometrically expands, and in response to the environment. Notably, strong correlations between the dissection a...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.15.01825

    authors: Chitwood DH,Rundell SM,Li DY,Woodford QL,Yu TT,Lopez JR,Greenblatt D,Kang J,Londo JP

    更新日期:2016-03-01 00:00:00

  • Effects of Previous Pollination and Stylar Ethylene on Pollen Tube Growth in Petunia hybrida Styles.

    abstract::The effect of ethylene on the growth rate of pollen tubes in styles of Petunia hybrida was examined. Apart from its strong inhibition of pollination-induced ethylene synthesis, aminoethoxyvinylglycine, placed on the stigma, did not impede tube growth. The inhibitors of the action of ethylene, silver thiosulfate and 2,...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.86.1.4

    authors: Hoekstra FA,van Roekel T

    更新日期:1988-01-01 00:00:00