The decisive step in betaxanthin biosynthesis is a spontaneous reaction1

Abstract:

:Experiments were performed to confirm that the aldimine bond formation is a spontaneous reaction, because attempts to find an enzyme catalyzing the last decisive step in betaxanthin biosynthesis, the aldimine formation, failed. Feeding different amino acids to betalain-forming hairy root cultures of yellow beet (Beta vulgaris L. subsp. vulgaris "Golden Beet") showed that all amino acids (S- and R-forms) led to the corresponding betaxanthins. We observed neither an amino acid specificity nor a stereoselectivity in this process. In addition, increasing the endogenous phenylalanine (Phe) level by feeding the Phe ammonia-lyase inhibitor 2-aminoindan 2-phosphonic acid yielded the Phe-derived betaxanthin. Feeding amino acids or 2-aminoindan 2-phosphonic acid to hypocotyls of fodder beet (B. vulgaris L. subsp. vulgaris "Altamo") plants led to the same results. Furthermore, feeding cyclo-3-(3,4-dihydroxyphenyl)-alanine (cyclo-Dopa) to these hypocotyls resulted in betanidin formation, indicating that the decisive step in betacyanin formation proceeds spontaneously. Finally, feeding betalamic acid to broad bean (Vicia faba L.) seedlings, which are known to accumulate high levels of Dopa but do not synthesize betaxanthins, resulted in the formation of dopaxanthin. These results indicate that the condensation of betalamic acid with amino acids (possibly including cyclo-Dopa or amines) in planta is a spontaneous, not an enzyme-catalyzed reaction.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Schliemann W,Kobayashi N,Strack D

doi

10.1104/pp.119.4.1217

subject

Has Abstract

pub_date

1999-04-01 00:00:00

pages

1217-32

issue

4

eissn

0032-0889

issn

1532-2548

journal_volume

119

pub_type

杂志文章
  • Relationship between Respiration and Photosynthesis in Guard Cell and Mesophyll Cell Protoplasts of Commelina communis L.

    abstract::A mass spectrometric method combining (16)O/(18)O and (12)C/(13)C isotopes was used to quantify the unidirectional fluxes of O(2) and CO(2) during a dark to light transition for guard cell protoplasts and mesophyll cell protoplasts of Commelina communis L. In darkness, O(2) uptake and CO(2) evolution were similar on a...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.95.2.636

    authors: Gautier H,Vavasseur A,Gans P,Lascève G

    更新日期:1991-02-01 00:00:00

  • Glutamine synthetase of pea leaves: divalent cation effects, substrate specificity, and other properties.

    abstract::Purified glutamine synthetase from pea seedlings was most active with Mg(2+) as the metal activator, but Mn(2+) and Co(2+) were 45 to 60% and 30 to 45% as effective, respectively, when assayed at the optimal pH for each cation. The Mg(2+) saturation curve was quite sigmoid, and evidence indicates that MgATP is the act...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.54.5.773

    authors: O'neal D,Joy KW

    更新日期:1974-11-01 00:00:00

  • Overexpression of ca1pase Decreases Rubisco Abundance and Grain Yield in Wheat.

    abstract::Rubisco catalyzes the fixation of CO2 into organic compounds that are used for plant growth and the production of agricultural products, and specific sugar-phosphate derivatives bind tightly to the active sites of Rubisco, locking the enzyme in a catalytically inactive conformation. 2-carboxy-d-arabinitol-1-phosphate ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.19.00693

    authors: Lobo AKM,Orr DJ,Gutierrez MO,Andralojc PJ,Sparks C,Parry MAJ,Carmo-Silva E

    更新日期:2019-10-01 00:00:00

  • Non-targeted and targeted protein movement through plasmodesmata in leaves in different developmental and physiological states.

    abstract::Plant cells rely on plasmodesmata for intercellular transport of small signaling molecules as well as larger informational macromolecules such as proteins. A green fluorescent protein (GFP) reporter and low-pressure microprojectile bombardment were used to quantify the degree of symplastic continuity between cells of ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.125.4.1802

    authors: Crawford KM,Zambryski PC

    更新日期:2001-04-01 00:00:00

  • Root Type-Specific Reprogramming of Maize Pericycle Transcriptomes by Local High Nitrate Results in Disparate Lateral Root Branching Patterns.

    abstract::The adaptability of root system architecture to unevenly distributed mineral nutrients in soil is a key determinant of plant performance. The molecular mechanisms underlying nitrate dependent plasticity of lateral root branching across the different root types of maize are only poorly understood. In this study, detail...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.15.01885

    authors: Yu P,Baldauf JA,Lithio A,Marcon C,Nettleton D,Li C,Hochholdinger F

    更新日期:2016-03-01 00:00:00

  • Characterization of MdMADS2, a member of the SQUAMOSA subfamily of genes, in apple.

    abstract::A MADS-box gene, MdMADS2, was isolated from the apple (Malus x domestica Borkh.) var Fuji and its developmental expression pattern was studied during flower development. MdMADS2 shares a high degree of amino acid sequence identity with the SQUAMOSA subfamily of genes. RNA blot analysis showed that MdMADS2 is transcrib...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.120.4.969

    authors: Sung SK,Yu GH,An G

    更新日期:1999-08-01 00:00:00

  • Medicago TERPENE SYNTHASE 10 Is Involved in Defense Against an Oomycete Root Pathogen.

    abstract::In nature, plants interact with numerous beneficial or pathogenic soil-borne microorganisms. Plants have developed various defense strategies to expel pathogenic microbes, some of which function soon after pathogen infection. We used Medicago truncatula and its oomycete pathogen Aphanomyces euteiches to elucidate earl...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.19.00278

    authors: Yadav H,Dreher D,Athmer B,Porzel A,Gavrin A,Baldermann S,Tissier A,Hause B

    更新日期:2019-07-01 00:00:00

  • Polygalacturonase-inhibiting protein interacts with pectin through a binding site formed by four clustered residues of arginine and lysine.

    abstract::Polygalacturonase-inhibiting protein (PGIP) is a cell wall protein that inhibits fungal polygalacturonases (PGs) and retards the invasion of plant tissues by phytopathogenic fungi. Here, we report the interaction of two PGIP isoforms from Phaseolus vulgaris (PvPGIP1 and PvPGIP2) with both polygalacturonic acid and cel...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.076950

    authors: Spadoni S,Zabotina O,Di Matteo A,Mikkelsen JD,Cervone F,De Lorenzo G,Mattei B,Bellincampi D

    更新日期:2006-06-01 00:00:00

  • Nitrogen Utilization in Lemna: I. Relations between Net Nitrate Flux, Nitrate Reduction, and in Vitro Activity and Stability of Nitrate Reductase.

    abstract::Cultures of Lemna gibba L. G3 were maintained at a constant, N-limited growth rate by adding nitrate daily in amounts calculated to sustain a rate of culture N increment of 0.20 day(-1). Nitrate added to the culture was consumed within 8 to 10 hours and the partitioning to reduction and accumulation during this phase ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.85.3.856

    authors: Ingemarsson B

    更新日期:1987-11-01 00:00:00

  • Thermal effect of CO(2) on apoplastic ice in rye and oat during freezing.

    abstract::Meristematic tissues from rye (Secale cereale) and oat (Avena sativa) were studied in an isothermal calorimeter at -3 degrees C. When the frozen tissue was placed in the calorimeter, the pressure increased within 4 d to 25 and 9 kPa above ambient pressure in the sample vessels containing crowns of rye and oat, respect...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.122.3.861

    authors: Livingston DP 3rd,Olien CR,Premakumar R

    更新日期:2000-03-01 00:00:00

  • A mutation in Arabidopsis seedling plastid development1 affects plastid differentiation in embryo-derived tissues during seedling growth.

    abstract::Oilseed plants like Arabidopsis (Arabidopsis thaliana) develop green photosynthetically active embryos. Upon seed maturation, the embryonic chloroplasts degenerate into a highly reduced plastid type called the eoplast. Upon germination, eoplasts redifferentiate into chloroplasts and other plastid types. Here, we descr...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.161414

    authors: Ruppel NJ,Logsdon CA,Whippo CW,Inoue K,Hangarter RP

    更新日期:2011-01-01 00:00:00

  • CML42-mediated calcium signaling coordinates responses to Spodoptera herbivory and abiotic stresses in Arabidopsis.

    abstract::In the interaction between Arabidopsis (Arabidopsis thaliana) and the generalist herbivorous insect Spodoptera littoralis, little is known about early events in defense signaling and their link to downstream phytohormone pathways. S. littoralis oral secretions induced both Ca²⁺ and phytohormone elevation in Arabidopsi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.198150

    authors: Vadassery J,Reichelt M,Hause B,Gershenzon J,Boland W,Mithöfer A

    更新日期:2012-07-01 00:00:00

  • Inhibition of photosynthetic electron transport by diphenyl ether herbicides.

    abstract::The effects of the diphenyl ether herbicides HOE 29152 (methyl-2[4-(4-trifluoromethoxy) phenoxy] propanoate) and nitrofluorfen (2-chloro-1-[4-nitrophenoxy]-4-[trifluoromethyl]benzene) on photosynthetic electron transport have been examined with pea seedling and spinach chloroplasts. Linear electron transport (water to...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.65.1.47

    authors: Bugg MW,Whitmarsh J,Rieck CE,Cohen WS

    更新日期:1980-01-01 00:00:00

  • The last step in cocaine biosynthesis is catalyzed by a BAHD acyltransferase.

    abstract::The esterification of methylecgonine (2-carbomethoxy-3β-tropine) with benzoic acid is the final step in the biosynthetic pathway leading to the production of cocaine in Erythoxylum coca. Here we report the identification of a member of the BAHD family of plant acyltransferases as cocaine synthase. The enzyme is capabl...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.114.248187

    authors: Schmidt GW,Jirschitzka J,Porta T,Reichelt M,Luck K,Torre JC,Dolke F,Varesio E,Hopfgartner G,Gershenzon J,D'Auria JC

    更新日期:2015-01-01 00:00:00

  • Role and interrelationship of Gα protein, hydrogen peroxide, and nitric oxide in ultraviolet B-induced stomatal closure in Arabidopsis leaves.

    abstract::Heterotrimeric G proteins have been shown to transmit ultraviolet B (UV-B) signals in mammalian cells, but whether they also transmit UV-B signals in plant cells is not clear. In this paper, we report that 0.5 W m(-2) UV-B induces stomatal closure in Arabidopsis (Arabidopsis thaliana) by eliciting a cascade of intrace...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.211623

    authors: He JM,Ma XG,Zhang Y,Sun TF,Xu FF,Chen YP,Liu X,Yue M

    更新日期:2013-03-01 00:00:00

  • Aryl hydroxylation of the herbicide diclofop by a wheat cytochrome p-450 monooxygenase : substrate specificity and physiological activity.

    abstract::Wheat (Triticum aestivum L. cv Etoile de Choisy) microsomes catalyzed the cytochrome P-450-dependent oxidation of the herbicide diclofop to three hydroxy-diclofop isomers. Hydroxylation was predominant at carbon 4, with migration of chlorine to carbon 5 (67%) and carbon 3 (25%). The 2,4-dichloro-5-hydroxy isomer was i...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.100.2.874

    authors: Zimmerlin A,Durst F

    更新日期:1992-10-01 00:00:00

  • Carbon Dioxide Exchange in Lichens : RELATIONSHIP BETWEEN NET PHOTOSYNTHETIC RATE AND CO(2) CONCENTRATION.

    abstract::The relationship between net photosynthesis and CO(2) concentration was investigated for four species of lichen using an infrared gas analyzer operating in a closed loop system. All species showed a linear relationship at low CO(2) levels (100 microliters per liter) with CO(2) saturation levels being in excess of 400 ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.68.1.199

    authors: Green TG,Snelgar WP

    更新日期:1981-07-01 00:00:00

  • Unique drought resistance functions of the highly ABA-induced clade A protein phosphatase 2Cs.

    abstract::Six Arabidopsis (Arabidopsis thaliana) clade A protein phosphatase 2Cs (PP2Cs) have established abscisic acid (ABA) signaling roles; however, phenotypic roles of the remaining three "HAI" PP2Cs, Highly ABA-Induced1 (HAI1), AKT1-Interacting PP2C1/HAI2, and HAI3, have remained unclear. HAI PP2C mutants had enhanced prol...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.202408

    authors: Bhaskara GB,Nguyen TT,Verslues PE

    更新日期:2012-09-01 00:00:00

  • Rapid Metabolic Changes in the Wounding Response of Leaf Discs following Excision.

    abstract::The dark respiration rate of discs from fully expanded tobacco leaves (Nicotiana tabacum) increased linearly with decreasing diameter, the relative increase being independent of leaf age. The wound respiration responsible for this situation reached a plateau within 15 minutes of excision. Metabolite analysis gave evid...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.57.1.80

    authors: Macnicol PK

    更新日期:1976-01-01 00:00:00

  • Rhythmicity in ethylene production in cotton seedlings.

    abstract::Cotyledons of cotton (Gossypium hirsutum L.) seedlings grown under a photoperiod of 12 hour darkness and 12 hour light showed daily oscillations in ethylene evolution. The rate of ethylene evolution began to increase toward the end of the dark period and reached a maximum rate during the first third of the light perio...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.75.2.493

    authors: Rikin A,Chalutz E,Anderson JD

    更新日期:1984-06-01 00:00:00

  • The Transcription Factor MYB29 Is a Regulator of ALTERNATIVE OXIDASE1a.

    abstract::Plants sense and integrate a variety of signals from the environment through different interacting signal transduction pathways that involve hormones and signaling molecules. Using ALTERNATIVE OXIDASE1a (AOX1a) gene expression as a model system of retrograde or stress signaling between mitochondria and the nucleus, MY...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.01494

    authors: Zhang X,Ivanova A,Vandepoele K,Radomiljac J,Van de Velde J,Berkowitz O,Willems P,Xu Y,Ng S,Van Aken O,Duncan O,Zhang B,Storme V,Chan KX,Vaneechoutte D,Pogson BJ,Van Breusegem F,Whelan J,De Clercq I

    更新日期:2017-03-01 00:00:00

  • Mesoporous silica nanoparticle-mediated intracellular cre protein delivery for maize genome editing via loxP site excision.

    abstract::The delivery of proteins instead of DNA into plant cells allows for a transient presence of the protein or enzyme that can be useful for biochemical analysis or genome modifications. This may be of particular interest for genome editing, because it can avoid DNA (transgene) integration into the genome and generate pre...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.233650

    authors: Martin-Ortigosa S,Peterson DJ,Valenstein JS,Lin VS,Trewyn BG,Lyznik LA,Wang K

    更新日期:2014-02-01 00:00:00

  • Factors involved in the opening of the hypocotyl hook of cotton and beans.

    abstract::Conditions influencing the opening of the bean (Phaseolus vulgaris L.) and cotton (Gossypium hirsutum L.) hypocotyl hook were defined. Such hooks were shown to undergo geotropic curvature; orientation of the hook with respect to gravity greatly affected the observed opening. Cotton and bean hooks behaved exactly oppos...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.45.5.548

    authors: Powell RD,Morgan PW

    更新日期:1970-05-01 00:00:00

  • Cytokinins of the Developing Mango Fruit : Isolation, Identification, and Changes in Levels during Maturation.

    abstract::The cytokinin activity has been isolated and identified from extracts of immature mango (Mangifera indica L.) seeds. The structures of zeatin, zeatin riboside, and N(6)-(Delta(2)-isopentenyl)adenine riboside were confirmed on the basis of their chromatographic behavior and mass spectra of trimethylsilyl derivatives. B...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.71.2.356

    authors: Chen WS

    更新日期:1983-02-01 00:00:00

  • Characteristics of tomato cell wall degradation in vitro: implications for the study of fruit-softening enzymes.

    abstract::The in vitro degradation of green tomato (Lycopersicon esculentum L.) cell walls by an extract of ripe fruits was characterized. The susceptibility of isolated walls to enzymolysis varied considerably among the different cultivars tested. Wall solubilization in vitro appeared to be nearly as extensive as that which ac...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.60.2.207

    authors: Wallner SJ,Bloom HL

    更新日期:1977-08-01 00:00:00

  • Colocalization of plastid division proteins in the chloroplast stromal compartment establishes a new functional relationship between FtsZ1 and FtsZ2 in higher plants.

    abstract::Chloroplast division is driven by a macromolecular complex containing components that are positioned on the cytosolic surface of the outer envelope, the stromal surface of the inner envelope, and in the intermembrane space. The only constituents of the division apparatus identified thus far are the tubulin-like protei...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:

    authors: McAndrew RS,Froehlich JE,Vitha S,Stokes KD,Osteryoung KW

    更新日期:2001-12-01 00:00:00

  • Metabolism of arginine by aging and 7 day old pumpkin seedlings.

    abstract::The metabolism of arginine by etiolated pumpkin (Cucurbita moschata) seedlings was studied over various time and age intervals by injecting arginine-U-(14)C into the cotyledons. At most, 25% of the (14)C was transported from the cotyledon to the axis tissue and the amount of this transport decreased with increasing ag...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.44.3.361

    authors: Splittstoesser WE

    更新日期:1969-03-01 00:00:00

  • Photosynthesis and inorganic carbon transport in isolated asparagus mesophyll cells.

    abstract::The possibility of HCO(3) (-) transport into isolated leaf mesophyll cells of Asparagus sprengeri Regel has been investigated. Measurement of the inorganic carbon pool in these cells over an external pH range 6.2 to 8.0, using the silicone-fluid filtration technique, indicated that the pool was larger than predicted b...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.70.3.649

    authors: Espie GS,Colman B

    更新日期:1982-09-01 00:00:00

  • WallGen, software to construct layered cellulose-hemicellulose networks and predict their small deformation mechanics.

    abstract::We understand few details about how the arrangement and interactions of cell wall polymers produce the mechanical properties of primary cell walls. Consequently, we cannot quantitatively assess if proposed wall structures are mechanically reasonable or assess the effectiveness of proposed mechanisms to change mechanic...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.146936

    authors: Kha H,Tuble SC,Kalyanasundaram S,Williamson RE

    更新日期:2010-02-01 00:00:00

  • delta-Aminolevulinic Acid Synthase of Euglena gracilis: Regulation of Activity.

    abstract::delta-Aminolevulinic acid (ALA), a key precursor of the tetrapyrroles heme and chlorophyll, is capable of being synthesized by two different routes in cells of the unicellular green alga Euglena gracilis: from the intact carbon skeleton of glutamate, and via the condensation of glycine and succinyl CoA, mediated by th...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.70.1.219

    authors: Foley T,Dzelzkalns V,Beale SI

    更新日期:1982-07-01 00:00:00