Abstract:
:Polygalacturonase-inhibiting protein (PGIP) is a cell wall protein that inhibits fungal polygalacturonases (PGs) and retards the invasion of plant tissues by phytopathogenic fungi. Here, we report the interaction of two PGIP isoforms from Phaseolus vulgaris (PvPGIP1 and PvPGIP2) with both polygalacturonic acid and cell wall fractions containing uronic acids. We identify in the three-dimensional structure of PvPGIP2 a motif of four clustered arginine and lysine residues (R183, R206, K230, and R252) responsible for this binding. The four residues were mutated and the protein variants were expressed in Pichia pastoris. The ability of both wild-type and mutated proteins to bind pectins was investigated by affinity chromatography. Single mutations impaired the binding and double mutations abolished the interaction, thus indicating that the four clustered residues form the pectin-binding site. Remarkably, the binding of PGIP to pectin is displaced in vitro by PGs, suggesting that PGIP interacts with pectin and PGs through overlapping although not identical regions. The specific interaction of PGIP with polygalacturonic acid may be strategic to protect pectins from the degrading activity of fungal PGs.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Spadoni S,Zabotina O,Di Matteo A,Mikkelsen JD,Cervone F,De Lorenzo G,Mattei B,Bellincampi Ddoi
10.1104/pp.106.076950subject
Has Abstractpub_date
2006-06-01 00:00:00pages
557-64issue
2eissn
0032-0889issn
1532-2548pii
pp.106.076950journal_volume
141pub_type
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