Polygalacturonase-inhibiting protein interacts with pectin through a binding site formed by four clustered residues of arginine and lysine.

Abstract:

:Polygalacturonase-inhibiting protein (PGIP) is a cell wall protein that inhibits fungal polygalacturonases (PGs) and retards the invasion of plant tissues by phytopathogenic fungi. Here, we report the interaction of two PGIP isoforms from Phaseolus vulgaris (PvPGIP1 and PvPGIP2) with both polygalacturonic acid and cell wall fractions containing uronic acids. We identify in the three-dimensional structure of PvPGIP2 a motif of four clustered arginine and lysine residues (R183, R206, K230, and R252) responsible for this binding. The four residues were mutated and the protein variants were expressed in Pichia pastoris. The ability of both wild-type and mutated proteins to bind pectins was investigated by affinity chromatography. Single mutations impaired the binding and double mutations abolished the interaction, thus indicating that the four clustered residues form the pectin-binding site. Remarkably, the binding of PGIP to pectin is displaced in vitro by PGs, suggesting that PGIP interacts with pectin and PGs through overlapping although not identical regions. The specific interaction of PGIP with polygalacturonic acid may be strategic to protect pectins from the degrading activity of fungal PGs.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Spadoni S,Zabotina O,Di Matteo A,Mikkelsen JD,Cervone F,De Lorenzo G,Mattei B,Bellincampi D

doi

10.1104/pp.106.076950

subject

Has Abstract

pub_date

2006-06-01 00:00:00

pages

557-64

issue

2

eissn

0032-0889

issn

1532-2548

pii

pp.106.076950

journal_volume

141

pub_type

杂志文章
  • Synthesis of Nitrate Reductase in Chlorella: I. EVIDENCE FOR AN INACTIVE PROTEIN PRECURSOR.

    abstract::Synthesis of nitrate reductase (EC 1.6.6.1) in Chlorella vulgaris was studied under inducing conditions, i.e. with cells grown on ammonia and then transferred to nitrate medium. Cycloheximide (but not chloramphenicol) completely inhibited synthesis of the enzyme, but only if it was added at the start (i.e. at the time...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.65.5.939

    authors: Funkhouser EA

    更新日期:1980-05-01 00:00:00

  • incurvata13, a novel allele of AUXIN RESISTANT6, reveals a specific role for auxin and the SCF complex in Arabidopsis embryogenesis, vascular specification, and leaf flatness.

    abstract::Auxin plays a pivotal role in plant development by modulating the activity of SCF ubiquitin ligase complexes. Here, we positionally cloned Arabidopsis (Arabidopsis thaliana) incurvata13 (icu13), a mutation that causes leaf hyponasty and reduces leaf venation pattern complexity and auxin responsiveness. We found that i...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.207779

    authors: Esteve-Bruna D,Pérez-Pérez JM,Ponce MR,Micol JL

    更新日期:2013-03-01 00:00:00

  • The MADS-domain protein AGAMOUS-like 15 accumulates in embryonic tissues with diverse origins.

    abstract::AGL15 (AGAMOUS-like 15), a member of the MADS-domain family of regulatory factors, accumulates preferentially in the organs and tissues derived from double fertilization in flowering plants (i.e. the embryo, suspensor, and endosperm). The developmental role of AGL15 is still undefined. If it is involved in embryogenes...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.120.1.121

    authors: Perry SE,Lehti MD,Fernandez DE

    更新日期:1999-05-01 00:00:00

  • Characterization of a molecular modification of phytochrome that is associated with its conversion to the far-red-absorbing form.

    abstract::Phytochrome that has been photoinduced to pellet by irradiation of intact oat (cv. Garry) shoots and recovered from a pellet obtained by centrifugation of crude extracts exhibits modified behavior when compared to soluble phytochrome isolated from shoots that had never been irradiated. This modified behavior includes ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.68.4.789

    authors: Boeshore ML,Pratt LH

    更新日期:1981-10-01 00:00:00

  • The alternative oxidase of plant mitochondria is involved in the acclimation of shoot growth at low temperature. A study of Arabidopsis AOX1a transgenic plants.

    abstract::The alternative oxidase (AOX) pathway of plant mitochondria uncouples respiration from mitochondrial ATP production and may ameliorate plant performance under stressful environmental conditions, such as cold temperatures, by preventing excess accumulation of reactive oxygen species. We tested this model in whole tissu...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.070789

    authors: Fiorani F,Umbach AL,Siedow JN

    更新日期:2005-12-01 00:00:00

  • Reaction Properties of the Ascorbic Acid Oxidase from Myrothecium verrucaria.

    abstract::Ascorbic acid oxidase activity in Myrothecium verrucaria extracts resulted in O(2) uptake exceeding 0.5 mole per mole of ascorbic acid and in CO(2) evolution. Measurement of oxidized ascorbic acid at completion of the reaction demonstrated that an average of 10% of the oxidized product disappeared. A comparison of the...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.41.10.1553

    authors: Lillehoj EB,Smith FG

    更新日期:1966-12-01 00:00:00

  • Heavy Metals Induce Iron Deficiency Responses at Different Hierarchic and Regulatory Levels.

    abstract::In plants, the excess of several heavy metals mimics iron (Fe) deficiency-induced chlorosis, indicating a disturbance in Fe homeostasis. To examine the level at which heavy metals interfere with Fe deficiency responses, we carried out an in-depth characterization of Fe-related physiological, regulatory, and morphologi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.01916

    authors: Lešková A,Giehl RFH,Hartmann A,Fargašová A,von Wirén N

    更新日期:2017-07-01 00:00:00

  • BOTRYTIS-INDUCED KINASE1 Modulates Arabidopsis Resistance to Green Peach Aphids via PHYTOALEXIN DEFICIENT4.

    abstract::BOTRYTIS-INDUCED KINASE1 (BIK1) plays important roles in induced defense against fungal and bacterial pathogens in Arabidopsis (Arabidopsis thaliana). Its tomato (Solanum lycopersicum) homolog is required for host plant resistance to a chewing insect herbivore. However, it remains unknown whether BIK1 functions in pla...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.114.242206

    authors: Lei J,A Finlayson S,Salzman RA,Shan L,Zhu-Salzman K

    更新日期:2014-08-01 00:00:00

  • Light-induced Changes of the Carotenoid Levels in Chloroplast Envelopes.

    abstract::The carotenoid content of thylakoids and envelopes isolated from dark-or light-treated spinach (Spinacia oleracea L.) chloroplasts was compared. In thylakoids, light induced a decrease of violaxanthin parallel with a stoichiometric increase of zeaxanthin due to violaxanthin deepoxidation. In envelopes, violaxanthin wa...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.61.4.530

    authors: Siefermann-Harms D

    更新日期:1978-04-01 00:00:00

  • A critical role of STAYGREEN/Mendel's I locus in controlling disease symptom development during Pseudomonas syringae pv tomato infection of Arabidopsis.

    abstract::Production of disease symptoms represents the final phase of infectious diseases and is a main cause of crop loss and/or marketability. However, little is known about the molecular basis of disease symptom development. In this study, a genetic screening was conducted to identify Arabidopsis (Arabidopsis thaliana) muta...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.111.181826

    authors: Mecey C,Hauck P,Trapp M,Pumplin N,Plovanich A,Yao J,He SY

    更新日期:2011-12-01 00:00:00

  • Phloem loading in two Scrophulariaceae species. What can drive symplastic flow via plasmodesmata?

    abstract::To determine the driving forces for symplastic sugar flux between mesophyll and phloem, gradients of sugar concentrations and osmotic pressure were studied in leaf tissues of two Scrophulariaceae species, Alonsoa meridionalis and Asarina barclaiana. A. meridionalis has a typical symplastic configuration of minor-vein ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.068312

    authors: Voitsekhovskaja OV,Koroleva OA,Batashev DR,Knop C,Tomos AD,Gamalei YV,Heldt HW,Lohaus G

    更新日期:2006-01-01 00:00:00

  • Analysis of ethylene signal-transduction kinetics associated with seedling-growth response and chitinase induction in wild-type and mutant arabidopsis.

    abstract::Kinetic aspects of ethylene-mediated signal transduction leading to seedling-growth inhibition and chitinase induction in Arabidopsis were investigated by the introduction of defined mutations in components of these pathways. Dose-response analysis of wild-type responses indicated that the rate-limiting steps for seed...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.2.597

    authors: Chen QG,Bleecker AB

    更新日期:1995-06-01 00:00:00

  • Identification of Putative Substrates of SEC2, a Chloroplast Inner Envelope Translocase.

    abstract::Most chloroplast proteins are synthesized in the cytosol and imported into chloroplasts. Many imported proteins are further targeted to the thylakoid membrane and lumen by the SEC1, TAT, or SRP/ALB3 translocases. Others are targeted to the inner chloroplast envelope membrane by undescribed translocases. Recently, a se...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.17.00012

    authors: Li Y,Martin JR,Aldama GA,Fernandez DE,Cline K

    更新日期:2017-04-01 00:00:00

  • Structure-activity relationship in the auxin activity of mono-substituted phenylacetic acids.

    abstract::The analysis of substituent constants for the lipophilic and electronic factors in the auxin activity of substituted phenylacetic acids in elongation of coleoptile segments shows that these factors parallel those for the phenoxyacetic acids but assign reactivity in growth promotion to the meta position of phenylacetic...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.42.11.1519

    authors: Muir RM,Fujita T,Hansch C

    更新日期:1967-11-01 00:00:00

  • Light-dependent and tissue-specific expression of the H-protein of the glycine decarboxylase complex.

    abstract::Glycine decarboxylase is a mitochondrial enzyme complex, which is the site of photorespiratory CO2 and NH3 release. Although the proteins that constitute the complex are located within the mitochondria, because of their intimate association with photosynthesis their expression is controlled by light. Comparisons of th...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.1.161

    authors: Srinivasan R,Oliver DJ

    更新日期:1995-09-01 00:00:00

  • CfrA, a Novel Carbon Flow Regulator, Adapts Carbon Metabolism to Nitrogen Deficiency in Cyanobacteria.

    abstract::Cyanobacteria unable to fix atmospheric nitrogen have evolved sophisticated adaptations to survive to long periods of nitrogen starvation. These genetic programs are still largely unknown-as evidenced by the many proteins whose expression is regulated in response to nitrogen availability, but which belong to unknown o...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.20.00802

    authors: Muro-Pastor MI,Cutillas-Farray Á,Pérez-Rodríguez L,Pérez-Saavedra J,Vega-de Armas A,Paredes A,Robles-Rengel R,Florencio FJ

    更新日期:2020-12-01 00:00:00

  • Rapid Metabolic Changes in the Wounding Response of Leaf Discs following Excision.

    abstract::The dark respiration rate of discs from fully expanded tobacco leaves (Nicotiana tabacum) increased linearly with decreasing diameter, the relative increase being independent of leaf age. The wound respiration responsible for this situation reached a plateau within 15 minutes of excision. Metabolite analysis gave evid...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.57.1.80

    authors: Macnicol PK

    更新日期:1976-01-01 00:00:00

  • Expression and localization of nitrilase during symptom development of the clubroot disease in Arabidopsis.

    abstract::The expression of nitrilase in Arabidopsis during the development of the clubroot disease caused by the obligate biotroph Plasmodiophora brassicae was investigated. A time course study showed that only during the exponential growth phase of the clubs was nitrilase prominently enhanced in infected roots compared with c...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.122.2.369

    authors: Grsic-Rausch S,Kobelt P,Siemens JM,Bischoff M,Ludwig-Müller J

    更新日期:2000-02-01 00:00:00

  • Target Molecular Size and Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis Analysis of the ATP-and Pyrophosphate-Dependent Proton Pumps from Maize Root Tonoplast.

    abstract::Tonoplast-enriched membranes were prepared from maize (Zea mays L. cv LG 11) primary roots, using sucrose nonlinear gradients. The functional molecular size of the tonoplast ATP-and PPi-dependent proton pumps were analyzed by radiation inactivation. Glucose-6-phosphate dehydrogenase (G6PDH) was added as an internal st...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.90.3.934

    authors: Chanson A,Pilet PE

    更新日期:1989-07-01 00:00:00

  • In VivoP NMR Spectroscopic Studies of Soybean Bradyrhizobium Symbiosis: I. Optimization of Parameters.

    abstract::(31)P NMR spectroscopy was used to study in vivo the symbiotic state established between soybean (Glycine max [L.] Merr. cv Williams) and Bradyrhizobium japonicum (USDA 110 and 138). Different experimental conditions were used to maintain perfused, respiring detached or attached nodules in an NMR magnet. The pH of the...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.89.4.1238

    authors: Rolin DB,Boswell RT,Sloger C,Tu SI,Pfeffer PE

    更新日期:1989-04-01 00:00:00

  • Identification and gene expression of anaerobically induced enolase in Echinochloa phyllopogon and Echinochloa crus-pavonis.

    abstract::Enolase (2-phospho-D-glycerate hydrolase, EC 4.2.1.11) has been identified as an anaerobic stress protein in Echinochloa oryzoides based on the homology of its internal amino acid sequence with those of enolases from other organisms, by immunological reactivity, and induction of catalytic activity during anaerobic str...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.2.433

    authors: Fox TC,Mujer CV,Andrews DL,Williams AS,Cobb BG,Kennedy RA,Rumpho ME

    更新日期:1995-10-01 00:00:00

  • CYP704B1 is a long-chain fatty acid omega-hydroxylase essential for sporopollenin synthesis in pollen of Arabidopsis.

    abstract::Sporopollenin is the major component of the outer pollen wall (exine). Fatty acid derivatives and phenolics are thought to be its monomeric building blocks, but the precise structure, biosynthetic route, and genetics of sporopollenin are poorly understood. Based on a phenotypic mutant screen in Arabidopsis (Arabidopsi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.144469

    authors: Dobritsa AA,Shrestha J,Morant M,Pinot F,Matsuno M,Swanson R,Møller BL,Preuss D

    更新日期:2009-10-01 00:00:00

  • Changes in the Content of Two Glutamate Synthase Proteins in Spikelets of Rice (Oryza sativa) Plants during Ripening.

    abstract::Nitrogen accumulation in the apical spikelets on the primary branches of the main stem of rice plants have been studied during the ripening process (0-35 d after flowering). The level of NADH-dependent glutamate synthase (GOGAT) protein and activity increased 4- and 6-fold, respectively, in the first 15 d after flower...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.101.4.1257

    authors: Hayakawa T,Yamaya T,Mae T,Ojima K

    更新日期:1993-04-01 00:00:00

  • Developmental Physiology of Bean Leaf Plastids II. Negative Contrast Electron Microscopy of Tubular Membranes in Prolamellar Bodies.

    abstract::Proplastids and prolamellar bodies with tubular membranes were isolated from the dark grown primary leaves of bean seedlings (Phaseolus vulgaris L.). The combination of fluorescence microscopy and negative contrast electron microscopy provided the tentative identification of protochlorophyll holochrome as a constituen...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.43.11.1769

    authors: Kahn A

    更新日期:1968-11-01 00:00:00

  • Age-induced protein modifications and increased proteolysis in potato seed-tubers

    abstract::Long-term aging of potato (Solanum tuberosum) seed-tubers resulted in a loss of patatin (40 kD) and a cysteine-proteinase inhibitor, potato multicystatin (PMC), as well as an increase in the activities of 84-, 95-, and 125-kD proteinases. Highly active, additional proteinases (75, 90, and 100 kD) appeared in the oldes...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.119.1.89

    authors: Kumar GN,Houtz RL,Knowles NR

    更新日期:1999-01-01 00:00:00

  • Effects of drought on primary photosynthetic processes of cotton leaves.

    abstract::The effects of drought on Photosystem II (PSII) fluorescence and photosynthetic electron transport activities were analyzed in cotton. Water stress did not modify the amplitude of leaf variable fluorescence at room temperature in the presence of 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) nor at 77 K. It is therefo...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.83.2.360

    authors: Genty B,Briantais JM,Da Silva JB

    更新日期:1987-02-01 00:00:00

  • Induction of Inorganic Carbon Accumulation in the Unicellular Green Algae Scenedesmus obliquus and Chlamydomonas reinhardtii.

    abstract::The induction of a dissolved inorganic carbon (DIC) accumulating mechanism in the two algal species Scenedesmus obliquus (WT) and Chlamydomonas reinhardtii (137 c+) was physiologically characterized by monitoring DIC uptake kinetics at a low and constant DIC concentration (120-140 micromolar), after transfer from high...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.87.2.437

    authors: Palmqvist K,Sjöberg S,Samuelsson G

    更新日期:1988-06-01 00:00:00

  • Unequal redundancy in maize knotted1 homeobox genes.

    abstract::The knotted1 (kn1) homeobox (knox) gene family was first identified through gain-of-function dominant mutants in maize (Zea mays). Class I knox members are expressed in meristems but excluded from leaves. In maize, a loss-of-function phenotype has only been characterized for kn1. To assess the function of another knox...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.228791

    authors: Bolduc N,Tyers RG,Freeling M,Hake S

    更新日期:2014-01-01 00:00:00

  • Turnover of dhurrin in green sorghum seedlings.

    abstract::The turnover of dhurrin in green seedlings of Sorghum bicolor (Linn) Moench var Redland x Greenleaf, Sudan 70 has been investigated using glyphosate and pulse-labeling studies with (14)C-tyrosine and [(14)C]shikimic acid. The rate of dhurrin breakdown was 4.8 nanomoles per hour in the shoot and 1.4 nanomoles per hour ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.94.3.1219

    authors: Adewusi SR

    更新日期:1990-11-01 00:00:00

  • Molecular and biochemical characterization of 2-hydroxyisoflavanone dehydratase. Involvement of carboxylesterase-like proteins in leguminous isoflavone biosynthesis.

    abstract::Isoflavonoids are ecophysiologically active secondary metabolites of the Leguminosae and known for health-promoting phytoestrogenic functions. Isoflavones are synthesized by 1,2-elimination of water from 2-hydroxyisoflavanones, the first intermediate with the isoflavonoid skeleton, but details of this dehydration have...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.056747

    authors: Akashi T,Aoki T,Ayabe S

    更新日期:2005-03-01 00:00:00