In VivoP NMR Spectroscopic Studies of Soybean Bradyrhizobium Symbiosis: I. Optimization of Parameters.

Abstract:

:(31)P NMR spectroscopy was used to study in vivo the symbiotic state established between soybean (Glycine max [L.] Merr. cv Williams) and Bradyrhizobium japonicum (USDA 110 and 138). Different experimental conditions were used to maintain perfused, respiring detached or attached nodules in an NMR magnet. The pH of the perfusion medium affected the cytoplasmic pH and the resolution of the spectra. The internal Pi content and distribution were assessed as a function of nodule age and green-house growth conditions and the rate of glucose and 2-deoxyglucose uptake into nodules in split and intact states. The major metabolites (glucose-6-P, fructose-1,6-diP, P-choline, Pi, NTP, UDP-glc, and NAD) were readily identified from (31)P NMR spectra of perchloric acid extracts of nodules with the exception of one unknown phosphorus metabolite. Nodules stressed by glucose deprivation demonstrated movement of Pi between the vacuole and cytoplasmic compartments not previously observed in (31)P NMR studies.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Rolin DB,Boswell RT,Sloger C,Tu SI,Pfeffer PE

doi

10.1104/pp.89.4.1238

subject

Has Abstract

pub_date

1989-04-01 00:00:00

pages

1238-46

issue

4

eissn

0032-0889

issn

1532-2548

journal_volume

89

pub_type

杂志文章
  • A survey of the pectic content of nonlignified monocot cell walls.

    abstract::The primary cell walls of graminaceous monocots were known to have a low content of pectin compared to those of dicots, but it was uncertain how widespread this feature was within the monocots as a whole. Nonlignified cell walls were therefore prepared from 33 monocot species for determination of their pectin content....

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.88.2.309

    authors: Jarvis MC,Forsyth W,Duncan HJ

    更新日期:1988-10-01 00:00:00

  • Down-regulation of caffeic acid o-methyltransferase in maize revisited using a transgenic approach.

    abstract::Transgenic maize (Zea mays) plants were generated with a construct harboring a maize caffeic acid O-methyltransferase (COMT) cDNA in the antisense (AS) orientation under the control of the maize Adh1 (alcohol dehydrogenase) promoter. Adh1-driven beta-glucuronidase expression was localized in vascular tissues and ligni...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.012237

    authors: Piquemal J,Chamayou S,Nadaud I,Beckert M,Barrière Y,Mila I,Lapierre C,Rigau J,Puigdomenech P,Jauneau A,Digonnet C,Boudet AM,Goffner D,Pichon M

    更新日期:2002-12-01 00:00:00

  • Parthenocarpic Fruit Development from Grafted Ovaries of Cucuminis sativus L.

    abstract::Reciprocal cleft and pistillate floral bud grafts were made between parthenocarpic ;Fertilla' and nonparthenocarpic ;MSU 713-5' cucumber (Cucumis sativus L.) lines to localize the site for stimulation of parthenocarpic fruit set. No fruit set on ;MSU 713-5' controls, scion grafted to ;Fertilla,' or rootstock with ;Fer...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.55.6.1107

    authors: Cantliffe DJ,Phatak SC

    更新日期:1975-06-01 00:00:00

  • Does ethylene play a role in the release of lateral buds (tillers) from apical dominance in oats?

    abstract::The growth of lateral buds (tillers), which are undergoing release from apical dominance, was measured in upright and gravistimulated intact Avena sativa L. cv. ;Victory' (oat) shoots as well as in isolated Avena stem segments treated with kinetin and sucrose. During release, the tiller bud initially shows a slow rate...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.70.3.811

    authors: Harrison MA,Kaufman PB

    更新日期:1982-09-01 00:00:00

  • Metabolism under Microaerobic Conditions of Mitochondria from Cowpea Nodules.

    abstract::A method is described for isolating mitochondria from nodules of cowpea (Vigna unguiculata [L.] Walp.) under completely anaerobic conditions. The mitochondria were immediately active when incubated aerobically with substrates, and their respiration rates were higher than mitochondria prepared in air. The mitochondria ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.81.4.1097

    authors: Rawsthorne S,Larue TA

    更新日期:1986-08-01 00:00:00

  • Genotypic variability in vulnerability of leaf xylem to cavitation in water-stressed and well-irrigated sugarcane.

    abstract::Genotypic variability in vulnerability of leaf xylem to water-stress-induced cavitation was determined in four sugarcane (Saccharum sp.) clones using detached leaf segments in a hydraulic conductivity apparatus. Vulnerability curves were constructed by plotting the percentage of maximum conductivity versus leaf water ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.100.2.1020

    authors: Neufeld HS,Grantz DA,Meinzer FC,Goldstein G,Crisosto GM,Crisosto C

    更新日期:1992-10-01 00:00:00

  • Short-Term Regulation of Crassulacean Acid Metabolism Activity in a Tropical Hemiepiphyte, Clusia uvitana.

    abstract::Diel courses of net CO2 exchange of leaves were studied in Clusia uvitana (Clusiaceae), a tropical Crassulacean acid metabolism (CAM) hemiepiphyte, growing in the crown of a 47-m tall kapok tree on Barro Colorado Island, Panama. Measurements on days without precipitation showed that net uptake of atmospheric CO2 occur...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.102.3.835

    authors: Zotz G,Winter K

    更新日期:1993-07-01 00:00:00

  • Maintenance of photosynthesis at low leaf water potential in wheat : role of potassium status and irrigation history.

    abstract::The interaction of low water potential effects on photosynthesis, and leaf K(+) levels in wheat (Triticum aestivum L.) plants was studied. Plants were grown at three K(+) fertilization levels; 0.2, 2, and 6 millimolar. With well watered plants, 2 millimolar K(+) supported maximal photosynthetic rates; 0.2 millimolar K...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.89.4.1358

    authors: Gupta AS,Berkowitz GA,Pier PA

    更新日期:1989-04-01 00:00:00

  • Effect of Specific Elicitors of Cladosporium fulvum on Tomato Suspension Cells : Evidence for the Involvement of Active Oxygen Species.

    abstract::Intercellular fluid (IF) obtained from tomato (Lycopersicon esculentum L.) leaflets colonized by Cladosporium fulvum Cooke contains specific elicitors that induce necrosis in tomato cultivars resistant to the race of C. fulvum used to produce the IF. The responses of cell-suspension cultures produced from tomato lines...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.99.3.1208

    authors: Vera-Estrella R,Blumwald E,Higgins VJ

    更新日期:1992-07-01 00:00:00

  • Growth and Development of Soybean (Glycine max [L.] Merr.) Pods: CO(2) Exchange and Enzyme Studies.

    abstract::The rates of CO(2) exchange and (14)CO(2) incorporation in the light and dark and the activities of several photosynthetic, photorespiratory, and respiratory enzymes of soybean (Glycine max [L.] Merr. cv. Wye) reproductive structures were determined at weekly intervals from anthesis to pod maturity. At all stages of p...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.55.4.745

    authors: Quebedeaux B,Chollet R

    更新日期:1975-04-01 00:00:00

  • Demonstration of Both a Photosynthetic and a Nonphotosynthetic CO(2) Requirement for NH(4) Assimilation in the Green Alga Selenastrum minutum.

    abstract::Nitrogen-limited and nitrogen-sufficient cell cultures of Selenastrum minutum (Naeg.) Collins (Chlorophyta) were used to investigate the dependence of NH(4) (+) assimilation on exogenous CO(2). N-sufficient cells were only able to assimilate NH(4) (+) maximally in the presence of CO(2) and light. Inhibition of photosy...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.95.1.192

    authors: Amory AM,Vanlerberghe GC,Turpin DH

    更新日期:1991-01-01 00:00:00

  • The effect of adenosine 5'-triphosphate on the shibata shift and on associated structural changes in the conformation of the prolamellar body in isolated maize etioplasts.

    abstract::Isolated maize (Zea mays var. kelvedon glory) etioplasts have been used to investigate the relationships between the spectral shifts and ultrastructural changes which occur during light-induced chloroplast development. After primary photoconversion, the Shibata shift was observed as a change from 680 to 670 nm in the ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.55.2.393

    authors: Horton P,Leech RM

    更新日期:1975-02-01 00:00:00

  • Ketose reductase activity in developing maize endosperm.

    abstract::Ketose reductase (NAD-dependent polyol dehydrogenase EC 1.1.1.14) activity, which catalyzes the NADH-dependent reduction of fructose to sorbitol (d-glucitol), was detected in developing maize (Zea mays L.) endosperm, purified 104-fold from this tissue, and partially characterized. Product analysis by high performance ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.84.3.830

    authors: Doehlert DC

    更新日期:1987-07-01 00:00:00

  • Synergistic biosynthesis of biphasic ethylene and reactive oxygen species in response to hemibiotrophic Phytophthora parasitica in tobacco plants.

    abstract::We observed the biphasic production of ethylene and reactive oxygen species (ROS) in susceptible tobacco (Nicotiana tabacum 'Wisconsin 38') plants after shoot inoculation with Phytophthora parasitica var nicotianae. The initial transient increase in ROS and ethylene at 1 and 3 h (phase I), respectively, was followed b...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.194654

    authors: Wi SJ,Ji NR,Park KY

    更新日期:2012-05-01 00:00:00

  • Induction of Male Sterility in Wheat by Meiotic-Stage Water Deficit Is Preceded by a Decline in Invertase Activity and Changes in Carbohydrate Metabolism in Anthers.

    abstract::Water deficit during meiosis in pollen mother cells of wheat (Triticum aestivum L.) induces male sterility, which can reduce grain set by 40 to 50%. In plants stressed during meiosis and then rewatered, division of pollen mother cells proceeds normally but subsequent pollen development is arrested 3 or 4 d later. An i...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.111.1.137

    authors: Dorion S,Lalonde S,Saini HS

    更新日期:1996-05-01 00:00:00

  • Analysis of the Nicotiana tabacum stigma/style transcriptome reveals gene expression differences between wet and dry stigma species.

    abstract::The success of plant reproduction depends on pollen-pistil interactions occurring at the stigma/style. These interactions vary depending on the stigma type: wet or dry. Tobacco (Nicotiana tabacum) represents a model of wet stigma, and its stigmas/styles express genes to accomplish the appropriate functions. For a larg...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.131573

    authors: Quiapim AC,Brito MS,Bernardes LA,Dasilva I,Malavazi I,DePaoli HC,Molfetta-Machado JB,Giuliatti S,Goldman GH,Goldman MH

    更新日期:2009-03-01 00:00:00

  • Protoplasts surviving freezing to -196 C and osmotic dehydration in 5 molar salt solutions prepared from the bark of winter black locust trees.

    abstract::Free protoplasts were prepared from the living bark tissue of the trunk of summer and winter black locust trees by enzymic digestion of thin slices of the tissue for 3 hours in a medium containing 2% Onozuka cellulase, 2% Rhozyme pectinase, and 2% Driselase in mannitol solutions using 0.4 molar mannitol for summer tis...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.63.4.722

    authors: Siminovitch D

    更新日期:1979-04-01 00:00:00

  • The Arabidopsis embryo mutant schlepperless has a defect in the chaperonin-60alpha gene.

    abstract::We identified a T-DNA-generated mutation in the chaperonin-60alpha gene of Arabidopsis that produces a defect in embryo development. The mutation, termed schlepperless (slp), causes retardation of embryo development before the heart stage, even though embryo morphology remains normal. Beyond the heart stage, the slp m...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.126.2.717

    authors: Apuya NR,Yadegari R,Fischer RL,Harada JJ,Zimmerman JL,Goldberg RB

    更新日期:2001-06-01 00:00:00

  • A Comparison of the Effect of Salt on Polypeptides and Translatable mRNAs in Roots of a Salt-Tolerant and a Salt-Sensitive Cultivar of Barley.

    abstract::The effect of salt stress on polypeptide and mRNA levels in roots of two barley (Hordeum vulgare L.) cultivars differing in salt tolerance (cv CM 72, tolerant; cv Prato, sensitive) was analyzed using two-dimensional polyacrylamide gel electrophoresis. Preliminary experiments indicated that germination of Prato was inh...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.90.4.1444

    authors: Hurkman WJ,Fornari CS,Tanaka CK

    更新日期:1989-08-01 00:00:00

  • Mathematical modeling-guided evaluation of biochemical, developmental, environmental, and genotypic determinants of essential oil composition and yield in peppermint leaves.

    abstract::We have previously reported the use of a combination of computational simulations and targeted experiments to build a first generation mathematical model of peppermint (Menthaxpiperita) essential oil biosynthesis. Here, we report on the expansion of this approach to identify the key factors controlling monoterpenoid e...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.152256

    authors: Rios-Estepa R,Lange I,Lee JM,Lange BM

    更新日期:2010-04-01 00:00:00

  • MEKK1 is required for flg22-induced MPK4 activation in Arabidopsis plants.

    abstract::The Arabidopsis (Arabidopsis thaliana) gene MEKK1 encodes a mitogen-activated protein kinase kinase kinase that has been implicated in the activation of the map kinases MPK3 and MPK6 in response to the flagellin elicitor peptide flg22. In this study, analysis of plants carrying T-DNA knockout alleles indicated that ME...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.091389

    authors: Suarez-Rodriguez MC,Adams-Phillips L,Liu Y,Wang H,Su SH,Jester PJ,Zhang S,Bent AF,Krysan PJ

    更新日期:2007-02-01 00:00:00

  • Subdivision of Light Signaling Networks Contributes to Partitioning of C4 Photosynthesis.

    abstract::Plants coordinate the expression of photosynthesis-related genes in response to growth and environmental changes. In species that conduct two-cell C4 photosynthesis, expression of photosynthesis genes is partitioned such that leaf mesophyll and bundle sheath cells accumulate different components of the photosynthetic ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.19.01053

    authors: Hendron RW,Kelly S

    更新日期:2020-03-01 00:00:00

  • l-Ornithine:2-Oxoacid Aminotransferase from Squash (Cucurbita pepo, L.) Cotyledons: Purification and Properties.

    abstract::ORNITHINE: 2-oxoacid aminotransferase (EC 2.6.1.13) has been purified over 400-fold with a total recovery of 14% from acetone powders of cotyledons of germinating squash (Cucurbita pepo, L.) seedlings. The pH optimum of the transamination between l-ornithine and alpha-ketoglutarate is 8 and the Michaelis constants are...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.55.3.502

    authors: Lu TS,Mazelis M

    更新日期:1975-03-01 00:00:00

  • Evidence for a Block between Plastoquinone and Cytochrome f in a Photosynthetic Mutant of Lemna with Abnormal Flowering Behavior.

    abstract::Mutant strain 1073 of Lemna perpusilla is concluded to be blocked between plastoquinone and cytochrome f in the photosynthetic electron transport system. The location of the block is based on the following observations of activities in chloroplasts isolated from the mutant and wild-type plants. (a) Relative to wild ty...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.57.4.577

    authors: Shahak Y,Posner HB,Avron M

    更新日期:1976-04-01 00:00:00

  • Identification of a specific isoform of tomato lipoxygenase (TomloxC) involved in the generation of fatty acid-derived flavor compounds.

    abstract::There are at least five lipoxygenases (TomloxA, TomloxB, TomloxC, TomloxD, and TomloxE) present in tomato (Lycopersicon esculentum Mill.) fruit, but their role in generation of fruit flavor volatiles has been unclear. To assess the physiological role of TomloxC in the generation of volatile C6 aldehyde and alcohol fla...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.041608

    authors: Chen G,Hackett R,Walker D,Taylor A,Lin Z,Grierson D

    更新日期:2004-09-01 00:00:00

  • Membrane glycerolipid remodeling triggered by nitrogen and phosphorus starvation in Phaeodactylum tricornutum.

    abstract::Diatoms constitute a major phylum of phytoplankton biodiversity in ocean water and freshwater ecosystems. They are known to respond to some chemical variations of the environment by the accumulation of triacylglycerol, but the relative changes occurring in membrane glycerolipids have not yet been studied. Our goal was...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.114.252395

    authors: Abida H,Dolch LJ,Meï C,Villanova V,Conte M,Block MA,Finazzi G,Bastien O,Tirichine L,Bowler C,Rébeillé F,Petroutsos D,Jouhet J,Maréchal E

    更新日期:2015-01-01 00:00:00

  • Studies in Wild Oat Seed Dormancy: II. ACTIVITIES OF PENTOSE PHOSPHATE PATHWAY DEHYDROGENASES.

    abstract::A selected strain of wild oat (Avena fatua L.) seed has been shown to lose dormancy rapidly during moist soil incubation at 25 C, whereas seed kept similarly at 5 C maintained a high level of dormancy.The activities of cytosolic dehydrogenase enzymes of the pentose phosphate pathway were assayed throughout a period of...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.68.1.15

    authors: Adkins SW,Ross JD

    更新日期:1981-07-01 00:00:00

  • Dead or Alive? Using Membrane Failure and Chlorophyll a Fluorescence to Predict Plant Mortality from Drought.

    abstract::Climate models predict widespread increases in both drought intensity and duration in the next decades. Although water deficiency is a significant determinant of plant survival, limited understanding of plant responses to extreme drought impedes forecasts of both forest and crop productivity under increasing aridity. ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.00581

    authors: Guadagno CR,Ewers BE,Speckman HN,Aston TL,Huhn BJ,DeVore SB,Ladwig JT,Strawn RN,Weinig C

    更新日期:2017-09-01 00:00:00

  • Aluminum resistance in the Arabidopsis mutant alr-104 is caused by an aluminum-induced increase in rhizosphere pH.

    abstract::A mechanism that confers increased Al resistance in the Arabidopsis thaliana mutant alr-104 was investigated. A modified vibrating microelectrode system was used to measure H+ fluxes generated along the surface of small Arabidopsis roots. In the absence of Al, no differences in root H+ fluxes between wild type and alr...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.117.1.19

    authors: Degenhardt J,Larsen PB,Howell SH,Kochian LV

    更新日期:1998-05-01 00:00:00

  • Contribution of Nonautotrophic Carbon Dioxide Fixation to Protein Synthesis in Suspension Cultures of Paul's Scarlet Rose.

    abstract::Bicarbonate-(14)C was provided to 5- and 11-day-old suspension cultures of Paul's Scarlet rose, and the incorporation of (14)C into lipid, protein, amino acids, and organic acids was determined. The rate of bicarbonate uptake was approximately the same by 5- and 11-day-old cells, but the distribution of (14)C among ce...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.55.4.643

    authors: Nesius KK,Fletcher JS

    更新日期:1975-04-01 00:00:00