Metabolism under Microaerobic Conditions of Mitochondria from Cowpea Nodules.

Abstract:

:A method is described for isolating mitochondria from nodules of cowpea (Vigna unguiculata [L.] Walp.) under completely anaerobic conditions. The mitochondria were immediately active when incubated aerobically with substrates, and their respiration rates were higher than mitochondria prepared in air. The mitochondria lacked fumarate reductase and were not inhibited by 5% CO(2). When incubated under microaerobic conditions, their respiration could be measured by leghemoglobin spectroscopy. Microaerobic respiration was inhibited approximately 50% by 1 millimolar malonate, and was completely inhibited by cyanide. O(2) uptake and the ATP/O ratio declined under microaerobic conditions, and therefore ATP production may be low in the environment of infected nodule cells.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Rawsthorne S,Larue TA

doi

10.1104/pp.81.4.1097

subject

Has Abstract

pub_date

1986-08-01 00:00:00

pages

1097-102

issue

4

eissn

0032-0889

issn

1532-2548

journal_volume

81

pub_type

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