Bicarbonate ion as a critical factor in photosynthetic oxygen evolution.

Abstract:

:Bicarbonate ion, not dissolved CO(2) gas, is shown to increase 4- to 5-fold the rate of dichlorophenol indophenol reduction by isolated maize (Zea mays) chloroplasts. Glutaraldehyde fixed chloroplasts continue to exhibit bicarbonate-dependent 2,6-dichlorophenol indophenol reduction. Bicarbonate is shown to act close to the oxygen-evolving site, i.e. prior to the electron donation site of diphenyl carbazide to photosystem II. Dark incubation and light pretreatment of chloroplasts in various concentrations of bicarbonate, just prior to assay, indicate that bicarbonate binds to chloroplasts in the dark and is released again as the Hill reaction proceeds in the light. It is suggested that bicarbonate ions may play a critical role in the oxygen-evolving process in photosynthesis.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Stemler A,Govindjee

doi

10.1104/pp.52.2.119

subject

Has Abstract

pub_date

1973-08-01 00:00:00

pages

119-23

issue

2

eissn

0032-0889

issn

1532-2548

journal_volume

52

pub_type

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