Absorption and distribution of high specific radioactivity 2-C-abscisic Acid in cotton seedlings.

Abstract:

:High specific radioactivity (26.3 mc/mmole) racemic 2-(14)C-abscisic acid was synthesized. An aliquot of abscisic acid, 1.2 x 10(-4)m in aqueous methanolic solution, was applied to the surface of either a cotyledon or the first true leaf of 8- to 32-day-old cotton seedlings (Gossypium hirsutum L.). After various intervals (6-192 hours), the seedlings were processed for autoradiography, counting, and identification of the radioactivity. After 6 hours, radioactivity was observed moving basipetally out of the treated leaf toward the roots. Four days later, radioactivity could be detected throughout the whole seedling. After 8 days, 10% of the recovered radioactivity was found in the roots, and 80% remained in the treated leaf blade. Neither leaf type nor age had any effect on the abscisic acid movement or pattern of distribution. Isolated radioactivity from the roots was identified as abscisic acid, based on comparison with an authentic standard by thin layer chromatography, gas-liquid chromatography, or gas-liquid chromatography-mass spectrometry.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Shindy WW,Asmundson CM,Smith OE,Kumamoto J

doi

10.1104/pp.52.5.443

subject

Has Abstract

pub_date

1973-11-01 00:00:00

pages

443-7

issue

5

eissn

0032-0889

issn

1532-2548

journal_volume

52

pub_type

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