A dual function alpha-dioxygenase-peroxidase and NAD(+) oxidoreductase active enzyme from germinating pea rationalizing alpha-oxidation of fatty acids in plants.

abstract：:The success of plant reproduction depends on pollen-pistil interactions occurring at the stigma/style. These interactions vary depending on the stigma type: wet or dry. Tobacco (Nicotiana tabacum) represents a model of wet stigma, and its stigmas/styles express genes to accomplish the appropriate functions. For a larg...

journal_title：Plant physiology

authors： Quiapim AC,Brito MS,Bernardes LA,Dasilva I,Malavazi I,DePaoli HC,Molfetta-Machado JB,Giuliatti S,Goldman GH,Goldman MH

更新日期：2009-03-01 00:00:00

abstract：:Biosynthesis of malate dehydrogenase isoenzymes was studied in cotyledons of watermelons (Citrullus vulgaris Schrad., var. Stone Mountain). The glyoxysomal and mitochondrial isoenzymes are synthesized as higher molecular weight precursors which can be immunoprecipitated by mono-specific antibodies from the products of...

journal_title：Plant physiology

authors： Gietl C,Hock B

更新日期：1982-08-01 00:00:00

abstract：:We describe the mechanical, electronic, hydraulic, and structural design of a nonrecirculating hydroponic system. The system is particularly suited to studies at very low nutrient concentrations, for which on-line concentration monitoring methods either do not exist or are costly and limited to monitoring relatively f...

journal_title：Plant physiology

authors： Gutschick VP,Kay LE

更新日期：1991-04-01 00:00:00

abstract：:Glyceraldehyde 3-phosphate dehydrogenases (EC 1.2.1.12 and 1.2.1.13) have been purified from the seed, root, etiolated, and green shoot of peas (Pisum sativum). These enzymes are tetramers of 140,000 daltons, with subunits of 35,000 daltons. The enzymes differ in isoelectric point. The seed enzyme has a pI of 5.1, and...

journal_title：Plant physiology

authors： McGowan RE,Gibbs M

更新日期：1974-09-01 00:00:00

abstract：:The Golgi apparatus in plant cells consists of a large number of independent Golgi stack/trans-Golgi network/Golgi matrix units that appear to be randomly distributed throughout the cytoplasm. To study the dynamic behavior of these Golgi units in living plant cells, we have cloned a cDNA from soybean (Glycine max), Gm...

journal_title：Plant physiology

authors： Nebenführ A,Gallagher LA,Dunahay TG,Frohlick JA,Mazurkiewicz AM,Meehl JB,Staehelin LA

更新日期：1999-12-01 00:00:00

abstract：:Changes in the levels of twenty-two free amino acids and in the amino acid composition of the total protein were measured throughout the development of cotyledons of a dwarf garden pea, Pisum sativum cv Greenfeast, grown in a constant environment. A sensitive double-isotope dansylation technique was used. Fresh weight...

journal_title：Plant physiology

authors： Macnicol PK

更新日期：1983-06-01 00:00:00

abstract：:Here, we monitor the effects of ectopic overexpression of genes for pea asparagine synthetase (AS1) in transgenic tobacco (Nicotiana tabacum). The AS genes of pea and tobacco are normally expressed only during the dark phase of the diurnal growth cycle and specifically in phloem cells. A hybrid gene was constructed in...

journal_title：Plant physiology

authors： Brears T,Liu C,Knight TJ,Coruzzi GM

更新日期：1993-12-01 00:00:00

abstract：:Germination of embryonic axes from dormant grain is inhibited by low concentrations of abscisic acid (ABA) compared with axes from nondormant grain. Incubation of dormant grain axes in 0.05 to 50 micromolar ABA caused the prolonged synthesis of a set of heat-stable proteins. Two of these proteins were identified as de...

journal_title：Plant physiology

authors： Ried JL,Walker-Simmons MK

更新日期：1990-06-01 00:00:00

abstract：:To understand the mechanism and molecular properties of the tonoplast-type H(+)-translocating ATPase, we have studied the effect of Cl(-), NO(3) (-), and 4,4'-diisothiocyano-2,2'-stilbene disulfonic acid (DIDS) on the activity of the electrogenic H(+)-ATPase associated with low-density microsomal vesicles from oat roo...

journal_title：Plant physiology

authors： Churchill KA,Sze H

更新日期：1984-10-01 00:00:00

abstract：:Barley (c.v. Himalaya) aleurone layers were incubated in [(3)H]gibberellin A(1) (GA(1)) at low temperatures. At 3 and 4 C, (3)H-activity was steadily accumulated in aleurone layers, and this accumulation was correlated with significant [(3)H]GA(1) metabolism. At 1 and 1.5 C, metabolism could not be detected, and at th...

journal_title：Plant physiology

authors： Keith B,Boal R,Srivastava LM

更新日期：1980-11-01 00:00:00

abstract：:To identify genetic loci involved in the regulation of organ-specific enzyme activities, a specific histochemical staining protocol was used in combination with quantitative trait locus (QTL) analysis. Using phosphoglucomutase (PGM) as an example, it is shown that enzyme activity can specifically, and with high resolu...

journal_title：Plant physiology

authors： Sergeeva LI,Vonk J,Keurentjes JJ,van der Plas LH,Koornneef M,Vreugdenhil D

更新日期：2004-01-01 00:00:00

abstract：:Flooding is a frequently occurring environmental stress that can severely affect plant growth. This study shows that treatment of corn (Zea mays L.) seedlings with abscisic acid (ABA) increases their tolerance to anoxia 10-fold over untreated seedlings and twofold over seedlings treated with water. Corn seedlings stre...

journal_title：Plant physiology

authors： Hwang SY,Vantoai TT

更新日期：1991-10-01 00:00:00

abstract：:Most chloroplast proteins are synthesized in the cytosol and imported into chloroplasts. Many imported proteins are further targeted to the thylakoid membrane and lumen by the SEC1, TAT, or SRP/ALB3 translocases. Others are targeted to the inner chloroplast envelope membrane by undescribed translocases. Recently, a se...

journal_title：Plant physiology

authors： Li Y,Martin JR,Aldama GA,Fernandez DE,Cline K

更新日期：2017-04-01 00:00:00

abstract：:A radioimmunoassay for glyceollin I, the major phytoalexin produced by soybean (Glycine max [L.] Merr.), has been developed. Antibodies were raised in rabbits against a glyceollin I-bovine serum albumin conjugate. The antisera were used to establish a radioimmunoassay for glyceollin I using [(125)I]glyceollin I as the...

journal_title：Plant physiology

authors： Moesta P,Hahn MG,Grisebach H

更新日期：1983-10-01 00:00:00

abstract：:Starch accumulation and sucrose synthesis and export were measured in leaves of sugar beet (Beta vulgaris L.) during a period of prolonged irradiance in which illumination was extended beyond the usual 14-hour day period. During much of the 14-hour day period, approximately 50% of the newly fixed carbon was distribute...

journal_title：Plant physiology

authors： Li B,Geiger DR,Shieh WJ

更新日期：1992-08-01 00:00:00

abstract：:Wood is an important raw material and environmentally cost-effective renewable source of energy. However, the molecular biology of wood formation (i.e. secondary growth) is surprisingly understudied. A novel experimental system was employed to study the molecular regulation of secondary xylem formation in Arabidopsis....

journal_title：Plant physiology

authors： Ko JH,Han KH,Park S,Yang J

更新日期：2004-06-01 00:00:00

abstract：:The degree of tubulin polymerization in cotton (Gossypium hirsutum L. cv Acala) cotyledonary tissue was estimated by radioimmunoassay which measured the amount of a tubulin-like factor. It was assumed that the release of this tubulin-like factor indicated depolymerization of microtubules. Exposure to chilling resulted...

journal_title：Plant physiology

authors： Rikin A,Atsmon D,Gitler C

更新日期：1983-04-01 00:00:00

abstract：:A method for testing the purity of isolated vacuoles is proposed. Preparations of isolated vacuoles often contain "impure vacuoles," namely, vacuoles contaminated with some cytoplasmic remnants. Such impure vacuoles are stained by the vital fluorescent dye fluorescein-diacetate. Vacuoles enclosed only by the tonoplast...

journal_title：Plant physiology

authors： Admon A,Jacoby B

更新日期：1980-01-01 00:00:00

abstract：:We screened a total of 1365 pea (Pisum sativum) lines for response to inoculation with Agrobacterium tumefaciens, strain B6, and characterized resistance in one cultivar, Sweet Snap. Sweet Snap seedlings were highly resistant to tumorigenesis under most conditions. Resistance was overcome at inoculum concentrations of...

journal_title：Plant physiology

authors： Robbs SL,Hawes MC,Lin HJ,Pueppke SG,Smith LY

更新日期：1991-01-01 00:00:00

abstract：:Aspen (Populus tremuloides Michx.) leaf extracts contain a newly discovered enzyme activity that catalyzes the magnesium ion-dependent elimination of diphosphate from dimethylallyl diphosphate with rearrangement to form isoprene (2-methyl, 1-3-butadiene). This isoprene synthase activity has been partially purified. Th...

journal_title：Plant physiology

authors： Silver GM,Fall R

更新日期：1991-12-01 00:00:00

abstract：:We found similarities between the effects of low night temperatures (5 degrees C-10 degrees C) and slowly imposed water stress on photosynthesis in grapevine (Vitis vinifera L.) leaves. Exposure of plants growing outdoors to successive chilling nights caused light- and CO(2)-saturated photosynthetic O(2) evolution to ...

journal_title：Plant physiology

authors： Flexas J,Badger M,Chow WS,Medrano H,Osmond CB

更新日期：1999-10-01 00:00:00

abstract：:Chlorophyll levels in l-cm sections of the youngest fully expanded leaves of normal (Y) Festuca pratensis L. declined almost to zero over a period of 6 days after excision. Chlorophyll in a mutant genotype (NY) remained near the initial level for the whole of this period. Abscisic acid promoted pigment loss in Y but h...

journal_title：Plant physiology

authors： Thomas H,Stoddart JL

更新日期：1975-09-01 00:00:00

abstract：:The rice plant (Oryza sativa L. cv Oochikara) is known to be a Si accumulator, but the mechanism responsible for the high uptake of Si by the roots is not well understood. We investigated the role of root hairs and lateral roots in the Si uptake using two mutants of rice, one defective in the formation of root hairs (...

journal_title：Plant physiology

authors： Ma JF,Goto S,Tamai K,Ichii M

更新日期：2001-12-01 00:00:00

abstract：:Nitrate releases seed dormancy in Arabidopsis (Arabidopsis thaliana) Columbia accession seeds in part by reducing abscisic acid (ABA) levels. Nitrate led to lower levels of ABA in imbibed seeds when included in the germination medium (exogenous nitrate). Nitrate also reduced ABA levels in dry seeds when provided to th...

journal_title：Plant physiology

authors： Matakiadis T,Alboresi A,Jikumaru Y,Tatematsu K,Pichon O,Renou JP,Kamiya Y,Nambara E,Truong HN

更新日期：2009-02-01 00:00:00

abstract：:Histone H2B monoubiquitination (H2Bub) is being recognized as a regulatory mechanism that controls a range of cellular processes in plants, but the molecular mechanisms of H2Bub that are involved in responses to biotic stress are largely unknown. In this study, we used wild-type and H2Bub loss-of-function mutations of...

journal_title：Plant physiology

authors： Hu M,Pei BL,Zhang LF,Li YZ

更新日期：2014-04-01 00:00:00

abstract：:Rice (Oryza sativa) accumulates prolamins and glutelins as storage proteins. The latter storage protein is synthesized on the endoplasmic reticulum (ER) as a 57-kD proglutelin precursor, which is then processed into acidic and basic subunits in the protein storage vacuole. Three esp2 mutants, CM1787, EM44, and EM747, ...

journal_title：Plant physiology

authors： Takemoto Y,Coughlan SJ,Okita TW,Satoh H,Ogawa M,Kumamaru T

更新日期：2002-04-01 00:00:00

abstract：:Protoplasts and intact chloroplasts isolated from Agropyron smithii Rybd. were utilized in an effort to determine the limiting factor(s) for photosynthesis at supraoptimal temperatures. Saturated CO(2)-dependent O(2) evolution had a temperature optimum of 35 degrees C for both protoplasts and intact chloroplasts. A sh...

journal_title：Plant physiology

authors： Kobza J,Uribe EG,Williams GJ

更新日期：1984-06-01 00:00:00

abstract：:Induction of ferric reductase activity in dicots and nongrass monocots is a well-recognized response to Fe deficiency. Recent evidence has shown that Cu deficiency also induces plasma membrane Fe reduction. In this study we investigated whether other nutrient deficiencies could also induce ferric reductase activity in...

journal_title：Plant physiology

authors： Cohen CK,Norvell WA,Kochian LV

更新日期：1997-07-01 00:00:00

abstract：:Light-mediated seedling development is coordinately controlled by a variety of key regulators. Here, we identified two B-box (BBX)-containing proteins, BBX30 and BBX31, as repressors of photomorphogenesis. ELONGATED HYPOCOTYL5, a central regulator of light signaling, directly binds to the G-box cis-element present in ...

journal_title：Plant physiology

authors： Heng Y,Lin F,Jiang Y,Ding M,Yan T,Lan H,Zhou H,Zhao X,Xu D,Deng XW

更新日期：2019-05-01 00:00:00

abstract：:The enzyme polyphenol oxidase (PPO) catalyzes the oxidation of phenolic compounds into highly reactive quinones. Polymerization of PPO-derived quinones causes the postharvest browning of cut or bruised fruit, but the native physiological functions of PPOs in undamaged, intact plant cells are not well understood. Walnu...

journal_title：Plant physiology

authors： Araji S,Grammer TA,Gertzen R,Anderson SD,Mikulic-Petkovsek M,Veberic R,Phu ML,Solar A,Leslie CA,Dandekar AM,Escobar MA

更新日期：2014-03-01 00:00:00