Abstract:
:(13)C nuclear magnetic resonance spectroscopy of intact leaves of Kalanchoë tubiflora was used to observe Crassulacean acid metabolism in vivo. (13)C signals from C-4 of malate were observed after overnight exposure of leaves to (13)CO(2). Illumination of the labeled leaves resulted in a gradual decrease in the malate signals. After a period of darkness in normal air, (13)C signals were detected in all four carbons of malate in the previously labeled leaves. The (13)C nuclear magnetic resonance spectrum of malate in solution was pH dependent, which allowed an estimation of the vacuolar pH from the whole leaf spectrum. The pH was 4.0 following a 14-hour dark period, but rose to greater than 6.0 after 6 hours of illumination.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Stidham MA,Moreland DE,Siedow JNdoi
10.1104/pp.73.2.517subject
Has Abstractpub_date
1983-10-01 00:00:00pages
517-20issue
2eissn
0032-0889issn
1532-2548journal_volume
73pub_type
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