Abstract:
:Dormancy is a complex evolutionary trait that temporally prevents seed germination, thus allowing seedling growth at a favorable season. High-throughput analyses of transcriptomes have led to significant progress in understanding the molecular regulation of this process, but the role of posttranscriptional mechanisms has received little attention. In this work, we have studied the dynamics of messenger RNA association with polysomes and compared the transcriptome with the translatome in dormant and nondormant seeds of Arabidopsis (Arabidopsis thaliana) during their imbibition at 25 °C in darkness, a temperature preventing germination of dormant seeds only. DNA microarray analysis revealed that 4,670 and 7,028 transcripts were differentially abundant in dormant and nondormant seeds in the transcriptome and the translatome, respectively. We show that there is no correlation between transcriptome and translatome and that germination regulation is also largely translational, implying a selective and dynamic recruitment of messenger RNAs to polysomes in both dormant and nondormant seeds. The study of 5' untranslated region features revealed that GC content and the number of upstream open reading frames could play a role in selective translation occurring during germination. Gene Ontology clustering showed that the functions of polysome-associated transcripts differed between dormant and nondormant seeds and revealed actors in seed dormancy and germination. In conclusion, our results demonstrate the essential role of selective polysome loading in this biological process.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Basbouss-Serhal I,Soubigou-Taconnat L,Bailly C,Leymarie Jdoi
10.1104/pp.15.00510subject
Has Abstractpub_date
2015-07-01 00:00:00pages
1049-65issue
3eissn
0032-0889issn
1532-2548pii
pp.15.00510journal_volume
168pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::At low water potential (psi(w)), dehydration reduces the symplast volume of leaf tissue. The effect of this reduction on photosynthetic capacity was investigated. The influence of osmotic adjustment on this relationship was also examined. To examine these relationships, comparative studies were undertaken on two wheat...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.85.4.1040
更新日期:1987-12-01 00:00:00
abstract::Wood is an important raw material and environmentally cost-effective renewable source of energy. However, the molecular biology of wood formation (i.e. secondary growth) is surprisingly understudied. A novel experimental system was employed to study the molecular regulation of secondary xylem formation in Arabidopsis....
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.038844
更新日期:2004-06-01 00:00:00
abstract::Changes in the properties of extractable vacuolar H+-pumping pyrophosphatase (V-PPase) and vacuolar ATPase activities in chilling-sensitive seedlings of mung bean (Vigna radiata) were investigated. Following chilling at 4[deg]C for 48 h, both hydrolytic and proton-pumping activities of the V-PPase increased 1.5- to 2-...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.2.659
更新日期:1995-10-01 00:00:00
abstract::Boron (B) polyol complexes have been isolated and characterized from the phloem sap of celery (Apium graveolens L.) and the extrafloral nectar of peach (Prunus persica L.). In celery the direct analysis of untreated phloem sap by matrix-assisted laser desorption-Fourier transform mass spectrometry, with verification b...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.2.649
更新日期:1997-02-01 00:00:00
abstract::Major storage reserves of Arabidopsis (Arabidopsis thaliana) seeds are triacylglycerols (seed oils) and proteins. Seed oil content is severely reduced for the regulatory mutant wrinkled1 (wri1-1; At3g54320) and for a double mutant in two isoforms of plastidic pyruvate kinase (pkpbeta(1)pkpalpha; At5g52920 and At3g2296...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.144121
更新日期:2009-11-01 00:00:00
abstract::Tonoplast vesicles were purified from a microsomal fraction isolated from etiolated soybean hypocotyls (Glycine max L.) by preparative free-flow electrophoresis. Marker enzyme determinations and immunoblot analysis against the vacuolar-ATPase confirmed the nature and the purity of the isolated membranes. A purified to...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.94.3.1149
更新日期:1990-11-01 00:00:00
abstract::The pool sizes of free l-phenylalanine and l-tyrosine, the precursors of rosmarinic acid in Anchusa officinalis L. cell suspension cultures, fluctuated during the culture cycle. The major increase in pool sizes was preceded by a peak of prephenate aminotransferase activity, while the subsequent decrease coincided with...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.89.2.429
更新日期:1989-02-01 00:00:00
abstract::Higher plants express several isoforms of vacuolar and cell wall invertases (CWI), some of which are inactivated by inhibitory proteins at certain stages of plant development. We have purified an apoplasmic inhibitor (INH) of tobacco (Nicotiana tabacum) CWI to homogeneity. Based on sequences from tryptic fragments, we...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.116.2.733
更新日期:1998-02-01 00:00:00
abstract::Rice (Oryza sativa) accumulates prolamins and glutelins as storage proteins. The latter storage protein is synthesized on the endoplasmic reticulum (ER) as a 57-kD proglutelin precursor, which is then processed into acidic and basic subunits in the protein storage vacuole. Three esp2 mutants, CM1787, EM44, and EM747, ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.010624
更新日期:2002-04-01 00:00:00
abstract::The chloroplast-associated form of superoxide dismutase from maize (Zea mays L.) (SOD-1) has been purified by a stepwise procedure consisting of (NH(4))(2)SO(4) fractionation, G-100 Sephadex gel filtration, DEAE-Sephacel chromatography, and hydroxylapatite chromatography. This procedure resulted in a single band on so...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.73.1.31
更新日期:1983-09-01 00:00:00
abstract::alpha-Amylases are important enzymes for starch degradation in plants. However, it has been a long-running debate as to whether alpha-amylases are localized in plastids where starch is stored. To study the subcellular localization of alpha-amylases in plant cells, a rice (Oryza sativa) alpha-amylase, alphaAmy3, with o...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.042184
更新日期:2004-07-01 00:00:00
abstract::A specific polysome immunoadsorption procedure, employing soluble rabbit anti-NADP-GDH IgG and sheep anti-rabbit IgG covalently-linked to an insoluble cellulose matrix, was used to immunoselect polysomes translating mRNA for a chloroplastic ammonium-inducible NADP-GDH in fully induced cells of Chlorella sorokiniana. T...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.81.2.527
更新日期:1986-06-01 00:00:00
abstract::Previous studies with isolated soybean cotyledon protoplasts revealed the presence of a saturable, simple diffusion, and nonsaturating carrier-mediated uptake of sucrose into soybean cotyledon cells. A proton/sucrose cotransport may be involved in the saturable sucrose uptake (Lin et al. 1984 Plant Physiol 75: 936-940...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.78.3.649
更新日期:1985-07-01 00:00:00
abstract::Levels of ethylene biosynthesis and ethylene-inducible gene expression in wild-type tomato (Lycopersicon esculentum) fruit and in nonripening fruit from the tomato mutant rin (ripening inhibitor) were compared in order to investigate the mechanism of ethylene action. Whereas wild-type tomato fruit dramatically increas...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.88.2.370
更新日期:1988-10-01 00:00:00
abstract::Two types of ATP-dependent calcium (Ca(2+)) transport systems were detected in sealed microsomal vesicles from oat roots. Approximately 80% of the total Ca(2+) uptake was associated with vesicles of 1.11 grams per cubic centimeter and was insensitive to vanadate or azide, but inhibited by NO(3) (-). The remaining 20% ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.79.4.1111
更新日期:1985-12-01 00:00:00
abstract::L-myo-inositol 1-phosphate synthase (EC 5.5.1.4) from cyanobacterial (Spirulina platensis), algal (Euglena gracilis), and higher plant (Oryza sativa, Vigna radiata) sources was purified to electrophoretic homogeneity, biochemically characterized, and compared. Both chloroplastic and cytosolic forms of the enzyme were ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.115.2.727
更新日期:1997-10-01 00:00:00
abstract::3-deoxy-D-manno-2-octulosonic acid-8-phosphate (Kdo-8-P) synthase catalyzes the condensation of phosphoenolpyruvate with D-arabinose-5-phosphate to yield Kdo-8-P. Kdo-8-P is the phosphorylated precursor of Kdo, a rare sugar only found in the rhamnogalacturonan II pectic fraction of the primary cell walls of higher pla...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.103.026872
更新日期:2003-09-01 00:00:00
abstract::The relationship between gravitropism and surface electrical potentials was studied using etiolated epicotyls of adzuki bean (Phaseolus angularis). Early downward curvature (or transient positive gravitropic response) was observed about 1 min after gravistimulation. The downward curvature was closely related to the sp...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.3.875
更新日期:1994-07-01 00:00:00
abstract::Six Arabidopsis (Arabidopsis thaliana) clade A protein phosphatase 2Cs (PP2Cs) have established abscisic acid (ABA) signaling roles; however, phenotypic roles of the remaining three "HAI" PP2Cs, Highly ABA-Induced1 (HAI1), AKT1-Interacting PP2C1/HAI2, and HAI3, have remained unclear. HAI PP2C mutants had enhanced prol...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.202408
更新日期:2012-09-01 00:00:00
abstract::Ice formation in stems, as determined by means of an electrophoretic mobility technique, occurs much more rapidly in azalea than in sour cherry. The difference is more marked in the bark than in the wood. Disrupting the structure of the tissues completely eliminates differences in freezing patterns, although gross ana...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.50.5.527
更新日期:1972-11-01 00:00:00
abstract::As regulators of gene expression in multicellular organisms, microRNAs (miRNAs) are crucial for growth and development. Although a plethora of factors involved in their biogenesis and action in Arabidopsis (Arabidopsis thaliana) has been described, these processes and their fine-tuning are not fully understood. Here, ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.17.01313
更新日期:2018-01-01 00:00:00
abstract::The esterification of methylecgonine (2-carbomethoxy-3β-tropine) with benzoic acid is the final step in the biosynthetic pathway leading to the production of cocaine in Erythoxylum coca. Here we report the identification of a member of the BAHD family of plant acyltransferases as cocaine synthase. The enzyme is capabl...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.114.248187
更新日期:2015-01-01 00:00:00
abstract::4-Hydroxyphenylpyruvate dioxygenase (4HPPD) catalyzes the formation of homogentisate (2,5-dihydroxyphenylacetate) from p-hydroxyphenylpyruvate and molecular oxygen. In plants this enzyme activity is involved in two distinct metabolic processes, the biosynthesis of prenylquinones and the catabolism of tyrosine. We repo...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.119.4.1507
更新日期:1999-04-01 00:00:00
abstract::Single-cell RNA sequencing (scRNA-seq) has been used extensively to study cell-specific gene expression in animals, but it has not been widely applied to plants. Here, we describe the use of a commercially available droplet-based microfluidics platform for high-throughput scRNA-seq to obtain single-cell transcriptomes...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.18.01482
更新日期:2019-04-01 00:00:00
abstract::Air pollutants are known to cause visible leaf injury as well as impairment of photosynthetic CO(2) fixation. Here we evaluate whether the effects on photosynthesis are large enough to cause changes in the relative composition of stable carbon isotopes, delta(13)C, of plant tissue samples, and, if so, how the changes ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.88.1.218
更新日期:1988-09-01 00:00:00
abstract::Vesicular-arbuscular mycorrhizal fungi are symbionts for a large variety of crop plants; however, the form in which they take up carbon from the host is not established. To trace the course of carbon metabolism, we have used nuclear magnetic resonance spectroscopy with [13C]glucose labeling in vivo and in extracts to ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.1.7
更新日期:1995-05-01 00:00:00
abstract::Stomata in epidermal strips of Vicia faba opened in light and closed in darkness when floated on dilute K(+) solutions. Opening and closing, respectively, paralleled the fluxes of labeled K(+) into and out of the strips. The gain and loss of K(+) by the strips were shown by colbaltinitrite stain to be centered at guar...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.46.3.483
更新日期:1970-09-01 00:00:00
abstract::Cuticular waxes are complex mixtures of very-long-chain fatty acids (VLCFAs) and their derivatives, forming a natural barrier on aerial surfaces of terrestrial plants against biotic and abiotic stresses. In VLCFA biosynthesis, β-ketoacyl-CoA synthase (KCS) is the key enzyme, catalyzing the first reaction in fatty acid...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.16.01527
更新日期:2017-02-01 00:00:00
abstract::Of the eight intermediates associated with the two pathways of UDP-d-glucuronic acid biosynthesis found in plants, only d-glucuronic acid inhibited myo-inositol 1-phosphate synthase (EC 5.5.1.4), formerly referred to as d-glucose 6-phosphate cycloaldolase. Inhibition was competitive. An attempt to demonstrate over-all...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.54.3.368
更新日期:1974-09-01 00:00:00
abstract::We studied the ethylene-insensitive, hypernodulating mutant, sickle (skl), to investigate the interaction of ethylene with auxin transport during root nodulation in Medicago truncatula. Grafting experiments demonstrated that hypernodulation in skl is root controlled. Long distance transport of auxin from shoot to root...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.080093
更新日期:2006-09-01 00:00:00