Cloning of a tobacco apoplasmic invertase inhibitor. Proof of function of the recombinant protein and expression analysis during plant development.

abstract：:The soluble bean leaf auxin-binding protein (ABP) has a high affinity for a range of auxins including indole-3-acetic acid (IAA), alpha-napthaleneacetic acid, phenylacetic acid, 2,4,5-trichlorophenoxyacetic acid, and structurally related auxins. A large number of nonauxin compounds that are nevertheless structurally r...

journal_title：Plant physiology

authors： Wardrop AJ,Polya GM

更新日期：1980-07-01 00:00:00

abstract：:LONG HYPOCOTYL5 (HY5) is a bZIP (basic leucine zipper) transcription factor that activates photomorphogenesis and root development in Arabidopsis (Arabidopsis thaliana). Previously, STF1 (soybean [Glycine max] TGACG-motif binding factor 1), a homologous legume protein with a RING-finger motif and a bZIP domain, was re...

journal_title：Plant physiology

authors： Song YH,Yoo CM,Hong AP,Kim SH,Jeong HJ,Shin SY,Kim HJ,Yun DJ,Lim CO,Bahk JD,Lee SY,Nagao RT,Key JL,Hong JC

更新日期：2008-04-01 00:00:00

abstract：:While the metabolic networks in developing seeds during the period of reserve accumulation have been extensively characterized, much less is known about those present during seed desiccation and subsequent germination. Here we utilized metabolite profiling, in conjunction with selective mRNA and physiological profilin...

journal_title：Plant physiology

authors： Fait A,Angelovici R,Less H,Ohad I,Urbanczyk-Wochniak E,Fernie AR,Galili G

更新日期：2006-11-01 00:00:00

abstract：:The rice plant (Oryza sativa L. cv Oochikara) is known to be a Si accumulator, but the mechanism responsible for the high uptake of Si by the roots is not well understood. We investigated the role of root hairs and lateral roots in the Si uptake using two mutants of rice, one defective in the formation of root hairs (...

journal_title：Plant physiology

authors： Ma JF,Goto S,Tamai K,Ichii M

更新日期：2001-12-01 00:00:00

abstract：:One of the most abundant proteins in maize (Zea mays L.) embryos is the molecular weight 63,000 globulin encoded by the Glb1 gene. To obtain DNA clones corresponding to Glb1, a cDNA library corresponding to RNA from developing maize embryos was constructed in a lambda expression vector and screened with antibodies spe...

journal_title：Plant physiology

authors： Belanger FC,Kriz AL

更新日期：1989-10-01 00:00:00

abstract：:The activity of 1,3-beta-d-glucan synthase assayed in the presence of digitonin in a microsomal preparation from suspension-cultured cells of Glycine max can be fully inhibited by unsaturated fatty acids, trienoic acids being most effective. Lysophosphatidylcholine, platelet-activating factor, acylcarnitine, and Echin...

journal_title：Plant physiology

authors： Kauss H,Jeblick W

更新日期：1986-01-01 00:00:00

abstract：:Porphobilinogen (PBG) deaminase catalyzes the polymerization of four PBG monopyrrole units into the linear tetrapyrrole hydroxymethylbilane necessary for the formation of chlorophyll and heme in plant cells. Degenerate oligonucleotide primers were designed based on amino acid sequence data (generated by mass spectrome...

journal_title：Plant physiology

authors： Witty M,Wallace-Cook AD,Albrecht H,Spano AJ,Michel H,Shabanowitz J,Hunt DF,Timko MP,Smith AG

更新日期：1993-09-01 00:00:00

abstract：:Flowering depends upon long-distance transport to supply water for reproductive mechanisms to function. Previous physiological studies suggested that flowers operated uncoupled from stem xylem transport and received water primarily from the phloem. We demonstrate that the water balance of Southern magnolia (Magnolia g...

journal_title：Plant physiology

authors： Feild TS,Chatelet DS,Brodribb TJ

更新日期：2009-07-01 00:00:00

abstract：:Numerous studies have demonstrated a rapid increase in the respiration rate during aging of slices of tuber and storage roots. To determine the molecular mechanisms of this phenomenon, the role of enzyme binding to the subcellular particulate fraction has been assessed in carrot (Daucus carota L.) and sugar beet (Beta...

journal_title：Plant physiology

authors： Moorhead GB,Plaxton WC

更新日期：1988-02-01 00:00:00

abstract：:A nucleoside triphosphatase (NTPase) present in highly purified preparations of pea nuclei was partially characterized. The activity of this enzyme was stimulated by divalent cations (Mg2+ = Mn2+ > Ca2+), but was not affected by the monovalent cations, Na+ and K+. The Mg(2+)-dependent activity was further stimulated...

journal_title：Plant physiology

authors： Chen YR,Roux SJ

更新日期：1986-01-01 00:00:00

abstract：:Despite much study of the role of S-adenosylmethionine (SAM) in the methylation of DNA, RNA, and proteins, and as a cofactor for a wide range of biosynthetic processes, little is known concerning the intracellular transport of this essential metabolite. Screening of the Arabidopsis (Arabidopsis thaliana) genome yielde...

journal_title：Plant physiology

authors： Palmieri L,Arrigoni R,Blanco E,Carrari F,Zanor MI,Studart-Guimaraes C,Fernie AR,Palmieri F

更新日期：2006-11-01 00:00:00

abstract：:The influence of indoleacetic acid, 0.03% CO(2), and malate on protein metabolism of etiolated Avena sativa coleoptile sections has been investigated. All three were found to elevate both the rate of incorporation of labeled leucine into protein, and the level of soluble protein. The combination of indoleacetic acid a...

journal_title：Plant physiology

authors： Bown AW,Aung T

更新日期：1974-07-01 00:00:00

abstract：:Chlorophyll degradation plays important roles in leaf senescence including regulation of degradation of chlorophyll-binding proteins. Although most genes encoding enzymes of the chlorophyll degradation pathway have been identified, the regulation of their activity has not been fully understood. Green cotyledon mutants...

journal_title：Plant physiology

authors： Kohzuma K,Sato Y,Ito H,Okuzaki A,Watanabe M,Kobayashi H,Nakano M,Yamatani H,Masuda Y,Nagashima Y,Fukuoka H,Yamada T,Kanazawa A,Kitamura K,Tabei Y,Ikeuchi M,Sakamoto W,Tanaka A,Kusaba M

更新日期：2017-04-01 00:00:00

abstract：:Chloroplast ribosomes, which originated from cyanobacteria, comprise a large subunit (50S) and a small subunit (30S) containing ribosomal RNAs (rRNAs) and various ribosomal proteins. Genes for many chloroplast ribosomal proteins, as well as proteins with auxiliary roles in ribosome biogenesis or functioning, reside in...

journal_title：Plant physiology

authors： Pulido P,Zagari N,Manavski N,Gawronski P,Matthes A,Scharff LB,Meurer J,Leister D

更新日期：2018-08-01 00:00:00

abstract：:Photosystem I (PSI) has been isolated from mesophyll chloroplasts of mature maize leaves. The isolated PSI (PSI-200) was used as starting material for preparing an antenna-depleted core (PSI-100). Both of these preparations appear to be quite analogous to PSI complexes isolated from other plant tissue sources, such as...

journal_title：Plant physiology

authors： Wynn RM,Luong C,Malkin R

更新日期：1989-09-01 00:00:00

abstract：:Isozymes of glutathione reductase (GR) have been purified from red spruce (Picea rubens Sarg.) needles. Two isozymes could be separated by anion-exchange chromatography from both nonhardened or cold-hardened tissue. Based on chromatographic elution profiles, the isozymes were designated GR-1NH and GR-2NH in preparatio...

journal_title：Plant physiology

authors： Hausladen A,Alscher RG

更新日期：1994-05-01 00:00:00

abstract：:Fusicoccin inhibits the germination of pea (Pisum sativum L. cv Progress 9) seeds by decreasing the growth of the embryonal axis and by stimulating the fresh weight increase of the cotyledons. The growth of isolated embryonal axes in the presence of sucrose and KCl is stimulated by fusicoccin. The effect of fusicoccin...

journal_title：Plant physiology

authors： Lioi L,Petruzzelli L,Morgutti S,Cocucci SM

更新日期：1984-11-01 00:00:00

abstract：:The genetic advantages to the use of Arabidopsis thaliana mutants for the study of auxin metabolism previously have been partially offset by the complexity of indolic metabolism in this plant and by the lack of proper methods. To address some of these problems, we developed isotopic labeling methods to determine amoun...

journal_title：Plant physiology

authors： Llić N,Normanly J,Cohen JD

更新日期：1996-07-01 00:00:00

abstract：:Histone H2B monoubiquitination (H2Bub1) plays critical roles in regulating growth and development as well as stress responses in Arabidopsis (Arabidopsis thaliana). In this study, we used wild-type and HUB1 and HUB2 loss-of-function Arabidopsis plants to elucidate the mechanisms involved in the regulation of the plant...

journal_title：Plant physiology

authors： Zhao J,Chen Q,Zhou S,Sun Y,Li X,Li Y

更新日期：2020-01-01 00:00:00

abstract：:The metabolism of soluble nucleotides was investigated in wheat (Triticum aestivum var. Olympic) aleurone layers treated with gibberellic acid. Whereas nucleotide levels were relatively unaffected by the treatment, a transient increase was observed in the incorporation of (32)P. The effect was maximal 30 to 60 minutes...

journal_title：Plant physiology

authors： Collins GG,Jenner CF,Paleg LG

更新日期：1972-03-01 00:00:00

abstract：:TILLING (for Targeting Induced Local Lesions IN Genomes) is a well-established method for identifying plants carrying point mutations in genes of interest. A traditional TILLING project requires a significant investment of time and resources to establish the mutant population and screening infrastructure. Here, we des...

journal_title：Plant physiology

authors： Bush SM,Krysan PJ

更新日期：2010-09-01 00:00:00

abstract：:Small GTP-binding proteins regulate diverse processes in eukaryotic cells such as signal transduction, cell proliferation, cytoskeletal organization, and intracellular membrane trafficking. These proteins function as molecular switches that cycle between "active" and "inactive" states, and this cycle is linked to the ...

journal_title：Plant physiology

authors： Vernoud V,Horton AC,Yang Z,Nielsen E

更新日期：2003-03-01 00:00:00

abstract：:Plant cells rely on plasmodesmata for intercellular transport of small signaling molecules as well as larger informational macromolecules such as proteins. A green fluorescent protein (GFP) reporter and low-pressure microprojectile bombardment were used to quantify the degree of symplastic continuity between cells of ...

journal_title：Plant physiology

authors： Crawford KM,Zambryski PC

更新日期：2001-04-01 00:00:00

abstract：:The rhythmic movement of excised Samanea saman pulvini incubated in H(2)O or 50 mm sucrose was monitored during extended periods of white light (cool white fluorescent, 2,000 ft-c), darkness, or alternating white light (16 hr) and darkness (8 hr). In continuous white light, the rhythm damps at an intermediate angle af...

journal_title：Plant physiology

authors： Simon E,Satter RL,Galston AW

更新日期：1976-09-01 00:00:00

abstract：:Carotenoids are essential pigments of the photosynthetic apparatus and an indispensable component of the human diet. In addition to being potent antioxidants, they also provide the vitamin A precursor beta-carotene. In tomato (Solanum lycopersicum) fruits, carotenoids accumulate in specialized plastids, the chromoplas...

journal_title：Plant physiology

authors： Apel W,Bock R

更新日期：2009-09-01 00:00:00

abstract：:Rice grains (Oryza sativa L.) from three varieties and three pairs of lines with different protein content were collected at 4-day intervals from 4 to 32 days after flowering. The samples were analyzed for protein, free amino nitrogen, ribonucleic acid, protease activity, and ribonuclease activity. In addition, the ca...

journal_title：Plant physiology

authors： Cruz LJ,Cagampang GB,Juliano BO

更新日期：1970-11-01 00:00:00

abstract：:Lipase (triacylglycerol acylhydrolase [EC 3.1.1.3.]) was extracted from the microsomal fraction of cotyledons of dark grown seedlings of Canola (Brassica napus L. cv Westar) by treatment with Triton X-100. The enzyme was partially purified by chromatography on Sephacryl S-300 and DEAE Bio-Gel and was stable when store...

journal_title：Plant physiology

authors： Weselake RJ,Thomson LW,Tenaschuk D,Mackenzie SL

更新日期：1989-12-01 00:00:00

abstract：:The decarboxylation of endogenous free glutamic acid by Chlorella pyrenoidosa, Marburg strain, was induced by a variety of metabolic poisons, by anaerobic conditions, and by freezing and thawing the cells. The rate of decarboxylation was proportional to the concentration of inhibitor present. Possible mechanisms which...

journal_title：Plant physiology

authors： Lane TR,Stiller M

更新日期：1970-05-01 00:00:00

abstract：:Under controlled environment and/or field conditions, vegetative growth (height, internode length, leaf area, shoot dry weight, grain yield) was greater in an F(1) maize hybrid than in either parental inbred. Endogenous gibberellin (GA)-like substances in apical meristem cylinders were also higher in the hybrid than i...

journal_title：Plant physiology

authors： Rood SB,Pharis RP,Koshioka M,Major DJ

更新日期：1983-03-01 00:00:00

abstract：:Superoxide dismutases (SODs) are ubiquitous metalloenzymes that catalyze the dismutation of superoxide radicals. Chloroplasts have two isozymes, copper/zinc SOD (Cu/ZnSOD) and iron SOD (FeSOD), encoded by nuclear genes. Because bryophytes are considered as the earliest land plants, they are one of the most interesting...

journal_title：Plant physiology

authors： Nagae M,Nakata M,Takahashi Y

更新日期：2008-04-01 00:00:00