Abstract:
:The genetic advantages to the use of Arabidopsis thaliana mutants for the study of auxin metabolism previously have been partially offset by the complexity of indolic metabolism in this plant and by the lack of proper methods. To address some of these problems, we developed isotopic labeling methods to determine amounts and examine the metabolism of indolic compounds in Arabidopsis. Isolation and indentification of endogenous indole-3-acetonitrile (IAN; a possible precursor of the auxin indole-3-acetic acid [IAA]) was carried out under mild conditions, thus proving its natural occurrence. We describe here the synthesis of 13C1-labeled IAN and its utility in the gas chromatography-mass spectrometry quantification of endogenous IAN levels. We also quantified the nonenzymatic conversion of IAN to IAA under conditions used to hydrolyze IAA conjugates. 13C1-Labeled IAN was used to assess the contribution of IAN to measured IAA following hydrolysis of IAA conjugates. We studied the stability and breakdown of the indolic glucosinolate glucobrassicin, which is known to be present in Arabidopsis. This is potentially an important concern when using Arabidopsis for studies of indolic biochemistry, since the levels of indolic auxins and auxin precursors are well below the levels of the indolic glucosinolates. We found that under conditions of extraction and base hydrolysis, formation of IAA from glucobrassicin was negligible.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Llić N,Normanly J,Cohen JDdoi
10.1104/pp.111.3.781subject
Has Abstractpub_date
1996-07-01 00:00:00pages
781-8issue
3eissn
0032-0889issn
1532-2548journal_volume
111pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::Samples of irradiated and control Avena sativa shoot tissue were homogenized together to determine whether, during homogenization phytochrome from irradiated tissue can bind to the particulate material simultaneously extracted from the control tissue. The level of phytochrome pelletability for such mixed tissue homoge...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.66.5.908
更新日期:1980-11-01 00:00:00
abstract::In this study, the pathway of β-citraurin biosynthesis, carotenoid contents and the expression of genes related to carotenoid metabolism were investigated in two varieties of Satsuma mandarin (Citrus unshiu), Yamashitabeni-wase, which accumulates β-citraurin predominantly, and Miyagawa-wase, which does not accumulate ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.223297
更新日期:2013-10-01 00:00:00
abstract::A rigid artificial osmotic cell has been constructed using reverse osmosis membranes that were supported by metal grids from both sides to yield a high elastic modulus of the system. The cell could be subjected to changes of external water potential either by evaporation or by application of hypertonic solutions so th...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.91.4.1454
更新日期:1989-12-01 00:00:00
abstract::The photochemistry of vesicular and detergent-solubilized preparations of plasma membrane-associated ATPase was investigated in Rosa damascena. The cholate-solubilized ATPase activity fractionated into two peaks on a Sephadex G-150 column with simple, but different ultraviolet (UV) sensitivities. The larger enzyme was...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.74.3.617
更新日期:1984-03-01 00:00:00
abstract::Dual-conditional positive/negative selection markers are versatile genetic tools for manipulating genomes. Plastid genomes are relatively small and conserved DNA molecules that can be manipulated precisely by homologous recombination. High-yield expression of recombinant products and maternal inheritance of plastid-en...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.204107
更新日期:2012-12-01 00:00:00
abstract::We previously reported (A Reinero, RN Beachy 1986 Plant Mol Biol 6:291-301) that coat protein (CP) of tobacco mosaic virus (TMV) accumulates in chloroplasts of systemically infected leaves. To determine the significance of such interaction we examined electron transport rates in chloroplasts containing different level...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.89.1.111
更新日期:1989-01-01 00:00:00
abstract::The growth of lateral buds (tillers), which are undergoing release from apical dominance, was measured in upright and gravistimulated intact Avena sativa L. cv. ;Victory' (oat) shoots as well as in isolated Avena stem segments treated with kinetin and sucrose. During release, the tiller bud initially shows a slow rate...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.70.3.811
更新日期:1982-09-01 00:00:00
abstract::Increased levels of solar ultraviolet (290-320 nm) (UV-B) radiation could have profound effects on plant proteins because the aromatic amino acids in proteins absorb strongly in this spectral region. We have investigated the effects of UV-B radiation on plant proteins and have observed a novel 66-kD protein. This prod...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.1.221
更新日期:1995-09-01 00:00:00
abstract::It is demonstrated that, in etiolated pea (Pisum sativum) epicotyls, ethylene affects the activation of both monomeric GTP-binding proteins (monomeric G-proteins) and protein kinases. For monomeric G-proteins, the effect may be a rapid (2 min) and bimodal up-regulation, a transiently unimodal activation, or a transien...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.102.015057
更新日期:2003-04-01 00:00:00
abstract::Apical dominance is the process whereby the shoot tip inhibits the growth of axillary buds along the stem. It has been proposed that the shoot tip, which is the predominant source of the plant hormone auxin, prevents bud outgrowth by suppressing auxin canalization and export from axillary buds into the main stem. In t...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.18.00519
更新日期:2019-01-01 00:00:00
abstract::Grain size is one of the key determinants of grain yield. Although a number of genes that control grain size in rice (Oryza sativa) have been identified, the overall regulatory networks behind this process remain poorly understood. Here, we report the map-based cloning and functional characterization of the quantitati...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.18.01574
更新日期:2019-08-01 00:00:00
abstract::Cool season crops face intermittent drought. Exposure to drought and other abiotic stresses is known to increase tolerance of the plants against subsequent exposure to such stresses. Storage of environmental signals is also proposed. Preexposure to a dehydration shock improved adaptive response during subsequent dehyd...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.043141
更新日期:2004-07-01 00:00:00
abstract::MicroRNAs (miRNAs) are approximately 21-nucleotide-long RNAs processed from nuclear-encoded transcripts, which include a characteristic hairpin-like structure. MiRNAs control the expression of target transcripts by binding to reverse complementary sequences directing cleavage or translational inhibition of the target ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.128025
更新日期:2008-10-01 00:00:00
abstract::The conversion of l-glutamate to delta-aminolevulinate, in preparations of cucumber etiochloroplasts incubated in vitro, was inhibited by protoheme IX and Mg-protoporphyrin IX. Mg-protoporphyrin IX was destroyed in the presence of air and plastids; this breakdown was accelerated by S-adenosyl methionine. Mg-protoporph...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.69.1.112
更新日期:1982-01-01 00:00:00
abstract::To test the hypothesis that multiple integrated root phenotypes would co-optimize drought tolerance, we phenotyped the root anatomy and architecture of 400 mature maize (Zea mays) genotypes under well-watered and water-stressed conditions in the field. We found substantial variation in all 23 root phenes measured. A p...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.20.00211
更新日期:2020-07-01 00:00:00
abstract::The phytohormones abscisic acid (ABA) and gibberellins (GAs) are the primary signals that regulate seed dormancy and germination. In this study, we investigated the role of a double APETALA2 repeat transcription factor, CHOTTO1 (CHO1), in seed dormancy, germination, and phytohormone metabolism of Arabidopsis (Arabidop...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.142018
更新日期:2009-10-01 00:00:00
abstract::Synechocystis PCC 6803 contains two types of glutathione peroxidase-like proteins (GPX-1 and GPX-2) that utilize NADPH but not reduced glutathione and unsaturated fatty acid hydroperoxides or alkyl hydroperoxides. The steady-state transcript level of gpx-1 gradually increased under oxidative stress conditions imposed ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.044842
更新日期:2004-09-01 00:00:00
abstract::We have isolated the plasma membrane H+-ATPase in a phosphorylated form from spinach (Spinacia oleracea L.) leaf tissue incubated with fusicoccin, a fungal toxin that induces irreversible binding of 14-3-3 protein to the C terminus of the H+-ATPase, thus activating H+ pumping. We have identified threonine-948, the sec...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.118.2.551
更新日期:1998-10-01 00:00:00
abstract::Mannitol-1-phosphate (M1P) dehydrogenase (M1PDH; EC 1.1.1.17), an enzyme catalyzing the reduction of Fru-6-phosphate (F6P) to M1P in algal mannitol biosynthesis, was purified to homogeneity from a cell homogenate of the eulittoral red alga Caloglossa continua (Okamura) King et Puttock. The enzyme was a monomer with an...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.103.026906
更新日期:2003-10-01 00:00:00
abstract::In nature, plants interact with numerous beneficial or pathogenic soil-borne microorganisms. Plants have developed various defense strategies to expel pathogenic microbes, some of which function soon after pathogen infection. We used Medicago truncatula and its oomycete pathogen Aphanomyces euteiches to elucidate earl...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.19.00278
更新日期:2019-07-01 00:00:00
abstract::The water fern, Azolla caroliniana Willd., containing the symbiotic, heterocystous blue-green alga, Anabaena azollae, has been studied under various growth conditions to characterize its light-dependent production of H(2). The response of H(2) production to N(2) and C(2)H(2) and the absence of a differential effect of...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.58.2.119
更新日期:1976-08-01 00:00:00
abstract::The mechanisms and the controlling factors of malate oxidation by mitochondria from leaves of Kalanchoë blossfeldiana Poelln. plants performing Crassulacean acid metabolism were investigated using Percollpurified mitochondria. The effects of pH and of various cofactors (ATP, NAD(+), coenzyme A) on malate dehydrogenase...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.81.4.1039
更新日期:1986-08-01 00:00:00
abstract::Plant architecture is determined by genetic and developmental programs as well as by environmental factors. Sessile plants have evolved a subtle adaptive mechanism that allows them to alter their growth and development during periods of stress. Phytohormones play a central role in this process; however, the molecules ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.146803
更新日期:2009-12-01 00:00:00
abstract::Mean inhibition of net photosynthesis in autotrophic tobacco callus by 21 and 40% O(2) was 30 and 47%, respectively, similar to intact leaves. Increasing CO(2) concentrations (500-2000 microliters per liter) produced a steady decline in percent inhibition at both O(2) concentrations, indicating that O(2) inhibition re...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.84.4.1055
更新日期:1987-08-01 00:00:00
abstract::A new bacterial agglutinin was isolated from seeds of the soybean cultivar Clark. Purification was carried out by ammonium sulfate precipitation and ion-exchange chromatography. The agglutinin is a heat-labile glycoprotein most active at pH 4.0. Addition of Ca(2+), Mn(2+) and Mg(2+) did not enhance the agglutinating a...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.66.5.847
更新日期:1980-11-01 00:00:00
abstract::Two isoforms of fructose 1,6-bisphosphatase are present in the green alga Selenastrum minutum. The isoenzymes can be separated with ionexchange chromatography or acid precipitation. The stability of the two isoenzymes differ largely. The acid insoluble enzyme exhibits properties similar to that of the enzyme from the ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.93.4.1460
更新日期:1990-08-01 00:00:00
abstract::All aerobic biological systems, including N(2)-fixing root nodules, are subject to O(2) toxicity that results from the formation of reactive intermediates such as H(2)O(2) and free radicals of O(2). H(2)O(2) may be removed from root nodules in a series of enzymic reactions involving ascorbate peroxidase, dehydroascorb...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.83.4.789
更新日期:1987-04-01 00:00:00
abstract::Using a novel screening procedure, we have selected a new class of mutant from the cyanobacterium Synechococcus PCC7942 that fails to adapt to growth at an extremely low inorganic carbon (Ci) concentration. The mutant (Tm17) reported in this study grows normally at or above air levels of CO2 (340 [mu]L L-1) but does n...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.2.605
更新日期:1994-02-01 00:00:00
abstract::Previous evidence has suggested that SPA1 is a signal transduction component that appears to require phytochrome A for function in seedling photomorphogenesis. Using digital image analysis, we examined the time course of growth inhibition induced by red light in spa1 mutants to test the interpretation that SPA1 functi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.126.3.1291
更新日期:2001-07-01 00:00:00
abstract::In Arabidopsis (Arabidopsis thaliana) the 466 pentatricopeptide repeat (PPR) proteins are putative RNA-binding proteins with essential roles in organelles. Roughly half of the PPR proteins form the plant combinatorial and modular protein (PCMP) subfamily, which is land-plant specific. PCMPs exhibit a large and variabl...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.077826
更新日期:2006-07-01 00:00:00