Abstract:
:Single-cell RNA sequencing (scRNA-seq) has been used extensively to study cell-specific gene expression in animals, but it has not been widely applied to plants. Here, we describe the use of a commercially available droplet-based microfluidics platform for high-throughput scRNA-seq to obtain single-cell transcriptomes from protoplasts of more than 10,000 Arabidopsis (Arabidopsis thaliana) root cells. We find that all major tissues and developmental stages are represented in this single-cell transcriptome population. Further, distinct subpopulations and rare cell types, including putative quiescent center cells, were identified. A focused analysis of root epidermal cell transcriptomes defined developmental trajectories for individual cells progressing from meristematic through mature stages of root-hair and nonhair cell differentiation. In addition, single-cell transcriptomes were obtained from root epidermis mutants, enabling a comparative analysis of gene expression at single-cell resolution and providing an unprecedented view of the impact of the mutated genes. Overall, this study demonstrates the feasibility and utility of scRNA-seq in plants and provides a first-generation gene expression map of the Arabidopsis root at single-cell resolution.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Ryu KH,Huang L,Kang HM,Schiefelbein Jdoi
10.1104/pp.18.01482subject
Has Abstractpub_date
2019-04-01 00:00:00pages
1444-1456issue
4eissn
0032-0889issn
1532-2548pii
pp.18.01482journal_volume
179pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::Constitutive arginase (molecular weight 330,000) 920-fold purified from Evernia prunastri thallus, is activated by putrescine, l-ornithine, and agmatine with K(a) values of 2.7, 1.1, and 5.8 millimolar, respectively. Constitutive arginase is also activated by endogenous l-arginine, reaching its maximum activity at 16 ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.76.4.1065
更新日期:1984-12-01 00:00:00
abstract::Extracts of the tendrils of Pisum sativum, Var. Alaska, exhibit adenosine triphosphatase activity which is inversely proportional to the amount the tendrils have coiled. The specific viscosity of the extract decreases when ATP is added. This evidence indicates a possible role of a contractile adenosine triphosphatase ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.42.6.845
更新日期:1967-06-01 00:00:00
abstract::Germination of embryonic axes from dormant grain is inhibited by low concentrations of abscisic acid (ABA) compared with axes from nondormant grain. Incubation of dormant grain axes in 0.05 to 50 micromolar ABA caused the prolonged synthesis of a set of heat-stable proteins. Two of these proteins were identified as de...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.93.2.662
更新日期:1990-06-01 00:00:00
abstract::The mechanism of cytokinin-induced cell expansion in cotyledons excised from dark-grown seedlings of radish (Raphanus sativus L.) and cucumber (Cucumus sativus L.) was studied. Cotyledons were incubated in dim light with or without 17 micromolar zeatin for periods up to 3 days. Fresh weights and osmotic potentials wer...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.68.1.107
更新日期:1981-07-01 00:00:00
abstract::Nitric oxide (NO) regulates multiple developmental events and stress responses in plants. A major biologically active species of NO is S-nitrosoglutathione (GSNO), which is irreversibly degraded by GSNO reductase (GSNOR). The major physiological effect of NO is protein S-nitrosylation, a redox-based posttranslational ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.15.00026
更新日期:2015-04-01 00:00:00
abstract::A physical analysis of water movement through elongating soybean (Glycine max L. Merr.) hypocotyls was made to determine why significant water potentials persist in growing tissues even though the external water potentials were zero and transpiration is virtually zero. The analysis was based on a water transport theor...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.62.3.423
更新日期:1978-09-01 00:00:00
abstract::Etiolated maize and soybean seedlings were grown for several days in atmospheres enriched with O(18). Hydroxyproline subsequently isolated from the seedlings by column and thin-layer chromatography was labeled with excess O(18), but proline was not. Control experiments in which seedlings were grown in H(2)O(18) and un...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.41.2.197
更新日期:1966-02-01 00:00:00
abstract::The microsomal fraction of avocado (Persea americana) mesocarp is a rich source of cytochrome P-450 active in the demethylation of xenobiotics. Cytochrome P-450 from this tissue has been purified and well characterized at the molecular level (DP O'Keefe, KJ Leto [1989] Plant Physiol 89: 1141-1149; KR Bozak, H Yu, R Si...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.98.4.1290
更新日期:1992-04-01 00:00:00
abstract::Crown galls induced by infection of tobacco plants with Agrobacterium tumefaciens strain C58-Cl(pTiB6S3) were excised and cultured in vitro. After about one year of culture on medium-lacking phytohormones, two noncloned lines spontaneously formed shoots. Leaf explants from shoots of tumor-line T5 were capable of growi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.69.1.37
更新日期:1982-01-01 00:00:00
abstract::ADP-glucose pyrophosphorylase catalyzes the synthesis of ADP-glucose (ADP-Glc) from Glc-1-phosphate (G-1-P) and ATP. Kinetic studies were performed to define the nature of the reaction, both in the presence and absence of allosteric effector molecules. When 3-phosphoglycerate (3-PGA), the putative physiological activa...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.149450
更新日期:2010-02-01 00:00:00
abstract::Hybrids between the C(4)-like species, Flaveria brownii, A. M. Powell and the C(3)-C(4) intermediate species Flaveria linearis Lag., Flaveria floridana Johnston, and Flaveria oppositifolia (DC.) Rydb. exhibited bivalent chromosome pairing during meiosis and stainability of pollen was high, ranging from 51 to 95%. An F...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.89.3.839
更新日期:1989-03-01 00:00:00
abstract::While it is apparent that the heat shock response is ubiquitous, variabilities in the nature of the heat shock response between closely related species have not been well characterized. The heat shock response of three genotypes of tomato, Lycopersicon esculentum, Lycopersicon pennellii, and the interspecific sexual h...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.93.3.1140
更新日期:1990-07-01 00:00:00
abstract::The release of fatty acids from membrane lipids has been implicated in various metabolic and physiological processes, but in many cases, the enzymes involved and their functions in plants remain unclear. Patatin-related phospholipase As (pPLAs) constitute a major family of acyl-hydrolyzing enzymes in plants. Here, we ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.216994
更新日期:2013-05-01 00:00:00
abstract::Cell-type-specific gene expression is essential to distinguish between the numerous cell types of multicellular organism. Therefore, cell-type-specific gene expression is tightly regulated and for most genes RNA transcription is the central point of control. Thus, transcriptional reporters are broadly used markers for...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.16.00213
更新日期:2016-06-01 00:00:00
abstract::The higher plant mitochondrial electron transport chain contains, in addition to the cytochrome chain, an alternative pathway that terminates with a single homodimeric protein, the alternative oxidase (AOX). We recorded temporary inhibition of cytochrome capacity respiration and activation of AOX pathway capacity in t...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.085472
更新日期:2006-10-01 00:00:00
abstract::The basic/helix-loop-helix (bHLH) transcription factors and their homologs form a large family in plant and animal genomes. They are known to play important roles in the specification of tissue types in animals. On the other hand, few plant bHLH proteins have been studied functionally. Recent completion of whole genom...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.080580
更新日期:2006-08-01 00:00:00
abstract::The O(2) concentration in intact and osmotically disrupted isolated spinach (Spinacia oleracea, L.) chloroplasts during photosynthesis was estimated. The chloroplasts were allowed to reduce 3-phosphoglycerate, CO(2), or ferricyanide in light until the rate of O(2) production was linear. When the light was turned off O...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.60.6.903
更新日期:1977-12-01 00:00:00
abstract::In flowering plant reproduction, pollen tube reception is the signaling system that results in pollen tube discharge, synergid degeneration, and successful delivery of male gametes (two sperm cells) to the site where they can fuse with female gametes (egg cell and central cell). Some molecules required for this comple...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.15.00528
更新日期:2015-09-01 00:00:00
abstract::Succulent stems of Cissus quadrangularis L. (Vitaceae) contain glutamine synthetase, glutamate synthase, and glutamate dehydrogenase. The CO(2) and water gas exchanges of detached internodes were typical for Crassulacean acid metabolism plants. During three physiological phases, e.g. in the dark, in the early illumina...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.81.2.356
更新日期:1986-06-01 00:00:00
abstract::Chlorophyll a fluorescence of Synechococcus UTEX 625 was quenched during the transport of inorganic carbon, even when CO(2) fixation was inhibited by iodoacetamide. Measurements with a pulse modulation fluorometer showed that at least 75% of the quenching was due to oxidation of Q(a), the primary acceptor of photosyst...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.88.1.6
更新日期:1988-09-01 00:00:00
abstract::Regulation of photosynthetic activity can contribute to the prevention of photodamage in stress resistant plants during exposure to drought or low temperatures. Responses to increasing levels of water stress were examined in seedlings of the stress resistant forest conifer, white spruce (Picea glauca [Moench.] Voss). ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.86.3.744
更新日期:1988-03-01 00:00:00
abstract::Large changes in indole-3-acetic acid (IAA) levels occur during growth of Lemna gibba G-3 in sterile culture. The levels of IAA were measured in plants during a 45 day growth cycle using HPLC and isotope dilution analysis followed by selected ion current monitoring GC-MS analysis with (13)C(6)-IAA as the internal stan...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.86.2.522
更新日期:1988-02-01 00:00:00
abstract::Dunaliella bardawil, a beta-carotene-accumulating halotolerant alga, has been analyzed for the effect of various growth conditions on its pigment content, and compared with Dunaliella salina, a beta-carotene nonaccumulating species. In D. bardawil, increasing light intensity and light period or inhibiting growth by va...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.72.3.593
更新日期:1983-07-01 00:00:00
abstract::An isotope-dilution method has been developed for the assay of free indole-3-acetic acid and ester indole-3-acetic acid as measured by indole-3-acetic acid liberated by mild alkaline hydrolysis. Application of this method to seedlings of Avena sativa and Zea mays indicates the upper limit of free indole-3-acetic acid ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.54.3.257
更新日期:1974-09-01 00:00:00
abstract::Ion transport, measured using double-barreled micropipettes to obtain current-voltage relations, was examined in Arabidopsis thaliana root hairs that continued tip growth and cytoplasmic streaming after impalement with the micropipette. To do this required in situ measurements with no handling of the seedlings to avoi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.97.4.1527
更新日期:1991-12-01 00:00:00
abstract::A toxic effect of alpha,alpha-trehalose in an angiospermic plant, Cuscuta reflexa (dodder), is described. This disaccharide and its analogs, 2-aminotrehalose and 4-aminotrehalose, induced a rapid blackening of the terminal region of the vine which is involved in elongation growth. From the results of in vitro growth o...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.68.6.1369
更新日期:1981-12-01 00:00:00
abstract::Despite a growing number of reports indicating non-Mendelian inheritance of transgene expression in monocots, no detailed description of the structure and stability of the transgene exists for transformants generated by direct DNA-transfer techniques, making the cause for these observations difficult to determine. In ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.115.2.361
更新日期:1997-10-01 00:00:00
abstract::Over recent years, a number of initiatives have proposed standard reporting guidelines for functional genomics experiments. Associated with these are data models that may be used as the basis of the design of software tools that store and transmit experiment data in standard formats. Central to the success of such dat...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.058875
更新日期:2005-05-01 00:00:00
abstract::Most dicotyledonous species respond to Fe deficiency by developing several mechanisms known as Fe-deficiency stress responses. To study the regulation of these responses, young cucumber plants (Cucumis sativus L. cv Ashley) were grown in nutrient solution for 11 d, being deprived of Fe during the last 4 or 5 d. Inhibi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.4.1133
更新日期:1994-08-01 00:00:00
abstract::The outermost cell layer of plants, the epidermis, and its outer (lateral) membrane domain facing the environment are continuously challenged by biotic and abiotic stresses. Therefore, the epidermis and the outer membrane domain provide important selective and protective barriers. However, only a small number of speci...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.16.01252
更新日期:2016-12-01 00:00:00