Characteristics of teratomas regenerated in vitro from octopine-type crown gall.

Abstract:

:Crown galls induced by infection of tobacco plants with Agrobacterium tumefaciens strain C58-Cl(pTiB6S3) were excised and cultured in vitro. After about one year of culture on medium-lacking phytohormones, two noncloned lines spontaneously formed shoots. Leaf explants from shoots of tumor-line T5 were capable of growing on hormone-free medium, and the resulting mixture of organized and unorganized tissue synthesized octopine. Detached leaves from T5 shoots also synthesized octopine. These results establish that shoots from this octopine-type tumor contain transformed cells and are true crown-gall teratomas.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Owens LD

doi

10.1104/pp.69.1.37

subject

Has Abstract

pub_date

1982-01-01 00:00:00

pages

37-40

issue

1

eissn

0032-0889

issn

1532-2548

journal_volume

69

pub_type

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