Ribulose 1,5-Bisphosphate Carboxylase Synthesis during Heat Shock.

Abstract:

:Ribulose 1,5-bisphosphate carboxylase (RuBPCase) was chosen as a model protein to study how heat shock (HS) affects both chloroplast protein synthesis and the nuclear-chloroplast interaction in production of chloroplast proteins. Experiments were performed using highly chlorophyllous, soybean (Glycine max L. Merr. var Corsoy) cell suspension cultures active in chloroplast protein synthesis. Synthesis of RuBPCase large (L) and small (S) subunits was followed by in vivo labeling, and corresponding mRNA levels were examined by Northern and dot hybridization analyses. Results demonstrate that L and S synthesis declines with increasing HS temperatures (33-40 degrees C) and reaches minimum levels (20-30% of control) at temperatures of maximum HS protein synthesis (39-40 degrees C). Recovery of L and S synthesis following a 2-hour HS at 38 or 40 degrees C was also studied. The changes in S synthesis during HS and recovery correlate with the steady state levels of S mRNA. In contrast, changes in L synthesis show little relationship to the corresponding mRNA levels; levels of L mRNA remain relatively unchanged by HS. We conclude that chloroplast protein synthesis shows no greater sensitivity to HS than is observed for cytoplasmic protein synthesis and that transport of proteins into the chloroplast (e.g.S subunit) continues during HS. Furthermore, there is no apparent coordination of L and S subunit mRNA levels under the conditions examined.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Vierling E,Key JL

doi

10.1104/pp.78.1.155

subject

Has Abstract

pub_date

1985-05-01 00:00:00

pages

155-62

issue

1

eissn

0032-0889

issn

1532-2548

journal_volume

78

pub_type

杂志文章
  • Uptake of bicarbonate ion in darkness by isolated chloroplast envelope membranes and intact chloroplasts of spinach.

    abstract::Bicarbonate uptake by isolated chloroplast envelope membranes and intact chloroplasts of spinach (Spinacia oleracea L. var. Viroflay) in darkness exhibited a similar dependency upon temperature, pH, time, and concentrations of isolated or attached envelope membranes. This similarity in uptake properties demonstrates t...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.54.4.520

    authors: Poincelot RP

    更新日期:1974-10-01 00:00:00

  • Synthesis and apparent turnover of Acid invertase in relation to invertase inhibitor in wounded sweet potato root tissue.

    abstract::Previously we showed that acid invertase activity increased and then decreased rapidly in wounded sweet potato (Ipomoea batatas Liam.) root tissue, and that the tissue contained a heat-stable, proteinaceous inhibitor with a molecular weight of about 19,500 daltons.In response to wounding of sweet potato root tissue, i...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.59.5.879

    authors: Matsushita K,Uritani I

    更新日期:1977-05-01 00:00:00

  • Comparison between a Stable NaCl-Selected Nicotiana Cell Line and the Wild Type : K, Na, and Proline Pools as a Function of Salinity.

    abstract::An NaCl-resistant line has been developed from suspension-cultured tobacco cells (Nicotiana tabacum/gossii) by stepwise increases in the NaCl concentration in the medium. Resistance showed stability through at least 24 generations in the absence of added NaCl.Above an external NaCl concentration of 35 millimolar, prol...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.73.3.624

    authors: Watad AE,Reinhold L,Lerner HR

    更新日期:1983-11-01 00:00:00

  • Photoperiodic Control of Flowering in Dark-Grown Seedlings of Pharbitis nil Choisy : The Effect of Skeleton and Continuous Light Photoperiods.

    abstract::The control of night-break timing was studied in dark-grown seedlings of Pharbitis nil (Choisy cv. Violet) following a single continuous or skeleton photoperiod. There was a rhythmic response to a red (R) interruption of an inductive dark period, and the phasing of the rhythm was influenced by the preceding light trea...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.70.1.277

    authors: Lumsden P,Thomas B,Vince-Prue D

    更新日期:1982-07-01 00:00:00

  • Partial Purification and Some Properties of Flavonol 7-Sulfotransferase from Flaveria bidentis.

    abstract::A novel flavonol-specific sulfotransferase was partially purified from the shoot tips of Flaveria bidentis var. Angustifolia O.K. (Asteraceae) by chromatography on 3'-phosphoadenosine 5'-phosphate-agarose affinity column and chromatofocusing on Mono P. The latter step resulted in the separation of two isoforms, both o...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.95.4.1254

    authors: Varin L,Ibrahim RK

    更新日期:1991-04-01 00:00:00

  • Differential expression of sucrose-phosphate synthase isoenzymes in tobacco reflects their functional specialization during dark-governed starch mobilization in source leaves.

    abstract::Sucrose (Suc)-phosphate synthase (SPS) plays a crucial role in the synthesis of Suc in photosynthetic and nonphotosynthetic tissues. Several isoforms of SPS exist in dicotyledonous plants that can be grouped into the different families A, B, and C. To explore whether functional differences between the SPS gene familie...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.069468

    authors: Chen S,Hajirezaei M,Börnke F

    更新日期:2005-11-01 00:00:00

  • Indole acetic acid distribution coincides with vascular differentiation pattern during Arabidopsis leaf ontogeny.

    abstract::We used an anti-indole acetic acid (IAA or auxin) monoclonal antibody-based immunocytochemical procedure to monitor IAA level in Arabidopsis tissues. Using immunocytochemistry and the IAA-driven beta-glucuronidase (GUS) activity of Aux/IAA promoter::GUS constructs to detect IAA distribution, we investigated the role o...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.003228

    authors: Avsian-Kretchmer O,Cheng JC,Chen L,Moctezuma E,Sung ZR

    更新日期:2002-09-01 00:00:00

  • Interaction between Arabidopsis Brca2 and its partners Rad51, Dmc1, and Dss1.

    abstract::The Arabidopsis (Arabidopsis thaliana) orthologs of Brca2, a protein whose mutations are involved in breast cancer in humans, were previously shown to be essential at meiosis. In an attempt to better understand the Brca2-interacting properties, we examined four partners of the two isoforms of Brca2 identified in Arabi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.075838

    authors: Dray E,Siaud N,Dubois E,Doutriaux MP

    更新日期:2006-03-01 00:00:00

  • Electrical evidence for turgor inhibition of proton extrusion in sugar beet taproot.

    abstract::Sections of sugar beet (Beta vulgaris L.) taproot were incubated in various concentrations of mannitol. At 0.4, 0.6, and 0.8 molar, the membrane electrical potential difference (E(m)) averaged about -130 millivolts; at 0.2 molar, about -90 millivolts; and at 0 molar, between -60 and -80 millivolts. Additions of 10 mil...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.82.4.1148

    authors: Kinraide TB,Wyse RE

    更新日期:1986-12-01 00:00:00

  • Overproduction in Escherichia coli and Characterization of a Soybean Ferric Leghemoglobin Reductase.

    abstract::We previously cloned and sequenced a cDNA encoding soybean ferric leghemoglobin reductase (FLbR), an enzyme postulated to play an important role in maintaining leghemoglobin in a functional ferrous state in nitrogen-fixing root nodules. This cDNA was sub-cloned into an expression plasmid, pTrcHis C, and overexpressed ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.1.203

    authors: Ji L,Becana M,Sarath G,Shearman L,Klucas RV

    更新日期:1994-09-01 00:00:00

  • Reduction in Pectin Methylesterase Activity Modifies Tissue Integrity and Cation Levels in Ripening Tomato (Lycopersicon esculentum Mill.) Fruits.

    abstract::Pectin methylesterase (PME, EC 3.1.1.11) is an ubiquitous enzyme in the plant kingdom; however, its role in plant growth and development is not yet understood. Using transgenic tomato (Lycopersicon esculentum Mill.) fruits that show more than 10-fold reduction in PME activity because of expression of an antisense PME ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.2.429

    authors: Tieman DM,Handa AK

    更新日期:1994-10-01 00:00:00

  • Water Relations and Growth of the flacca Tomato Mutant in Relation to Abscisic Acid.

    abstract::The flacca mutant in tomato (Lycopersicon esculentum Mill. cv Rheinlands Ruhm) was employed to examine the effects of a relatively constant diurnal water stress on leaf growth and water relations. As the mutant is deficient in abscisic acid (ABA) and can be phenotypically reverted to the wild type by applications of t...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.72.1.251

    authors: Bradford KJ

    更新日期:1983-05-01 00:00:00

  • Cellular distribution of polysome populations of castor bean endosperm.

    abstract::The distribution of polysomes in the cells of the endosperm of very young (48 hours of germination) seedlings of castor bean (Ricinus communis var. Hale) has been examined. Seedlings exposed to 100 micromolar gibberellin A(3) for periods up to 24 hours showed increased numbers of polysomes associated with endomembrane...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.65.2.359

    authors: González E

    更新日期:1980-02-01 00:00:00

  • Five Nodulation Mutants of White Sweetclover (Melilotus alba Desr.) Exhibit Distinct Phenotypes Blocked at Root Hair Curling, Infection Thread Development, and Nodule Organogenesis.

    abstract::In an effort to obtain a developmental sequence of mutations in the Rhizobium-legume interaction within a single legume species, we have characterized the early events of nodule development in 10 nodulation mutants of sweetclover, Melilotus alba Desr. cv U389, representing five genetic loci. Both seed and root exudate...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.3.925

    authors: Utrup LJ,Cary AJ,Norris JH

    更新日期:1993-11-01 00:00:00

  • The rib1 mutant is resistant to indole-3-butyric acid, an endogenous auxin in Arabidopsis.

    abstract::The presence of indole-3-butyric acid (IBA) as an endogenous auxin in Arabidopsis has been recently demonstrated. However, the in vivo role of IBA remains to be elucidated. We present the characterization of a semi-dominant mutant that is affected in its response to IBA, but shows a wild-type response to indole-3-acet...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.124.4.1739

    authors: Poupart J,Waddell CS

    更新日期:2000-12-01 00:00:00

  • Reactive oxygen species are involved in gibberellin/abscisic acid signaling in barley aleurone cells.

    abstract::Reactive oxygen species (ROS) act as signal molecules for a variety of processes in plants. However, many questions about the roles of ROS in plants remain to be clarified. Here, we report the role of ROS in gibberellin (GA) and abscisic acid (ABA) signaling in barley (Hordeum vulgare) aleurone cells. The production o...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.111.192740

    authors: Ishibashi Y,Tawaratsumida T,Kondo K,Kasa S,Sakamoto M,Aoki N,Zheng SH,Yuasa T,Iwaya-Inoue M

    更新日期:2012-04-01 00:00:00

  • Chloroplast glutathione reductase.

    abstract::Glutathione reductase (EC 1.6.4.2) activity is present in spinach (Spinacia oleracea L.) chloroplasts. The pH dependence and substrate concentration for half-maximal rate are reported and a possible role in chloroplasts is proposed. ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.59.5.1011

    authors: Schaedle M

    更新日期:1977-05-01 00:00:00

  • A novel role for light in the activation of ribulosebisphosphate carboxylase/oxygenase.

    abstract::Light stimulated the activation of ribulosebisphosphate carboxylase/oxygenase (rubisco) in a buffered lysed chloroplast system in the presence of saturating concentrations of ATP. This indicates a role for light in the rubisco activase activation system in addition to the previously identified requirement for the synt...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.92.1.110

    authors: Campbell WJ,Ogren WL

    更新日期:1990-01-01 00:00:00

  • Cloning of a tobacco apoplasmic invertase inhibitor. Proof of function of the recombinant protein and expression analysis during plant development.

    abstract::Higher plants express several isoforms of vacuolar and cell wall invertases (CWI), some of which are inactivated by inhibitory proteins at certain stages of plant development. We have purified an apoplasmic inhibitor (INH) of tobacco (Nicotiana tabacum) CWI to homogeneity. Based on sequences from tryptic fragments, we...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.116.2.733

    authors: Greiner S,Krausgrill S,Rausch T

    更新日期:1998-02-01 00:00:00

  • A simple procedure to overcome polyethelene glycol toxicity on whole plants.

    abstract::A procedure is described that can be used to minimize toxic effects of polyethylene glycol (PEG) to plants. The procedure is based on recycling nutrient solutions containing PEG-6000 through two plant cultures. Tomato plants grown in -0.3 megapascals PEG solutions used after two growth cycles exhibited minimal toxic e...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.79.2.559

    authors: Plaut Z,Federman E

    更新日期:1985-10-01 00:00:00

  • Pentose phosphate metabolism of potato tuber discs as influenced by prior storage temperature.

    abstract::Tubers of Solanum tuberosum L. var. Russet Burbank, Kennebec, and Targhee were stored at 95% relative humidity and at 1.7, 4.4, 5.8, 7.2 or 10 C. Rates of O(2) consumption were measured periodically by removing individual tubers from storage, excising discs of pith parenchyma tissue, and incubating the discs in a Gils...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.61.2.252

    authors: Dwelle RB,Stallknecht GF

    更新日期:1978-02-01 00:00:00

  • Comparative Expression Profiling Reveals Genes Involved in Megasporogenesis.

    abstract::Megasporogenesis is a key step during ovule development in angiosperms, but the small number and inaccessibility of these cells have hampered molecular and genome-wide studies. Thus, many questions remain regarding the molecular basis of cell specification, differentiation, and development in the female gametophyte. H...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.19.01254

    authors: Zhao H,Guo M,Yan M,Cheng H,Liu Y,She Z,Lai L,Shi C,Zhang M,Li Y,Lin D,Qin Y

    更新日期:2020-04-01 00:00:00

  • Proteome analysis. Novel proteins identified at the peribacteroid membrane from Lotus japonicus root nodules.

    abstract::The peribacteroid membrane (PBM) forms the structural and functional interface between the legume plant and the rhizobia. The model legume Lotus japonicus was chosen to study the proteins present at the PBM by proteome analysis. PBM was purified from root nodules by an aqueous polymer two-phase system. Extracted prote...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.102.015362

    authors: Wienkoop S,Saalbach G

    更新日期:2003-03-01 00:00:00

  • 5-Aminolevulinate Synthesis in Permeabilized Filaments of the Blue-Green Alga Anabaena variabilis.

    abstract::Filaments of the blue-green alga Anabaena variabilis permeabilized by dimethylsulfoxide (DMSO) produce increased amounts of 5-aminolevulinate in the presence of levulinic acid. The metabolic activity of the filaments remains unperturbed in the presence of up to 7.5% (v/v) DMSO. Studies utilizing DMSO-permeabilized fil...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.72.1.200

    authors: Avissar YJ

    更新日期:1983-05-01 00:00:00

  • Parthenocarpic Fruit Development from Grafted Ovaries of Cucuminis sativus L.

    abstract::Reciprocal cleft and pistillate floral bud grafts were made between parthenocarpic ;Fertilla' and nonparthenocarpic ;MSU 713-5' cucumber (Cucumis sativus L.) lines to localize the site for stimulation of parthenocarpic fruit set. No fruit set on ;MSU 713-5' controls, scion grafted to ;Fertilla,' or rootstock with ;Fer...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.55.6.1107

    authors: Cantliffe DJ,Phatak SC

    更新日期:1975-06-01 00:00:00

  • Abscisic Acid promotes both volume flow and ion release to the xylem in sunflower roots.

    abstract::The effect of abscisic acid (ABA) on the exudation process in excised sunflower root was investigated. A promotion of both ion flux and volume exudation rate was observed. Cutting off the external supply of ions to the roots markedly increased the magnitude of the ABA effect. The promotive effect of ABA on exudation r...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.65.3.537

    authors: Glinka Z

    更新日期:1980-03-01 00:00:00

  • Carotenogenesis Is Regulated by 5'UTR-Mediated Translation of Phytoene Synthase Splice Variants.

    abstract::Phytoene synthase (PSY) catalyzes the highly regulated, frequently rate-limiting synthesis of the first biosynthetically formed carotene. While PSY constitutes a small gene family in most plant taxa, the Brassicaceae, including Arabidopsis (Arabidopsis thaliana), predominantly possess a single PSY gene. This monogenic...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.01262

    authors: Álvarez D,Voß B,Maass D,Wüst F,Schaub P,Beyer P,Welsch R

    更新日期:2016-12-01 00:00:00

  • Phytochrome-mediated Induction of Phenylalanine Ammonia-Lyase in Mustard Seedlings: A Contribution to Eliminate Some Misconceptions.

    abstract::The present report shows that the effect of red and far red light on the synthesis of phenylalanine ammonia-lyase can be ascribed to the action of phytochrome Pfr. This is true not only for short term irradiation but also for the action of continuous far red light. In the latter case, the model worked out by Hartmann ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.49.1.8

    authors: Schopfer P,Mohr H

    更新日期:1972-01-01 00:00:00

  • The effect of 1,4-dihydropyridines on the initiation and development of gametophore buds in the moss funaria.

    abstract::The plant hormone cytokinin stimulates target caulonemata of Funaria to form buds that develop into the leafy gametophyte. Previous reports have shown that increases in intracellular Ca(2+) occur during hormone-activated budding concomitant with an alteration in the polarity of the organelles in the bud site. In order...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.86.3.684

    authors: Conrad PA,Hepler PK

    更新日期:1988-03-01 00:00:00

  • C Nuclear Magnetic Resonance Studies of Crassulacean Acid Metabolism in Intact Leaves of Kalanchoë tubiflora.

    abstract::(13)C nuclear magnetic resonance spectroscopy of intact leaves of Kalanchoë tubiflora was used to observe Crassulacean acid metabolism in vivo. (13)C signals from C-4 of malate were observed after overnight exposure of leaves to (13)CO(2). Illumination of the labeled leaves resulted in a gradual decrease in the malate...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.73.2.517

    authors: Stidham MA,Moreland DE,Siedow JN

    更新日期:1983-10-01 00:00:00