Abstract:
:The transfer of Nicotiana tabacum plants grown in low light (60 micromoles quanta per square meter per second) to higher light (360 micromoles quanta per square meter per second) was previously shown to induce adaptive stimulation of photosynthetic capacities. The variations of ribulose bisphosphate carboxylase/oxygenase (RubisCo) expression in mature leaves was examined as a result of this acclimation. Maximum or initial activities increased markedly after low- to high-light transfer with a maximum effect after 2 to 3 days. The higher activity is mainly explained by RubisCo protein synthesis as shown by immunorocket technique. Small subunits of RubisCo (SSU) mRNA relative content determined by hybridization of total RNA with DNA probe by Dot-blot method, followed the same pattern as RubisCo quantity. The magnitude of this response was amplified when more contrasting light conditions (25 versus 360 micromoles per square meter per second) were established on the same leaf: RubisCo activity, RubisCo protein, and SSU mRNA contents decreased in the shaded zone and increased in the high-light zone within 1 day. After 2 days the shade/light ratio was 1 to 3 for RubisCo protein and 1 to 4 for SSU-RNA, whereas the ratios remained equal to one in controls. Hybridization of the same RNA extracts with large subunits of RubisCo (LSU) probe showed no variation in LSU-RNA content. So in green adult leaves, the expression of SSU and LSU genes is regulated differently. The observed white light quantitative effect on RubisCo expression was not dependent on the photosynthetic rate or assimilate content since low CO(2) concentration around the leaf after the light shift did not modify the response.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Prioul JL,Reyss Adoi
10.1104/pp.84.4.1238subject
Has Abstractpub_date
1987-08-01 00:00:00pages
1238-43issue
4eissn
0032-0889issn
1532-2548journal_volume
84pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::Lemna paucicostata Hegelm. 6746 possesses specific systems for uptake of choline and ethanolamine. Each is distinct from the six other systems for uptake of organic compounds so far identified in this plant. Both systems show biphasic kinetics, so that uptake by them can be described as the composite result of two Mic...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.81.1.285
更新日期:1986-05-01 00:00:00
abstract::Cadmium complexes with apparent molecular weights of 10,000 were observed in aqueous extracts of Cd-treated cabbage (Brassica capitata L., cv. red danish) and tobacco (hybrid of Nicotiana glauca and N. langsdorffii) plants. The amount of complex (as Cd) recovered was found to be dependent on the concentration of the m...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.69.4.804
更新日期:1982-04-01 00:00:00
abstract::Polyadenylated (poly A(+)) RNAs were prepared from both dry and incubated embryonic axes of Vigna unguiculata seeds and were translated by a wheat germ translation system. Analysis with gel electrophoresis and fluorography showed that translation products of poly A(+) RNA from dry embryonic axes were nearly the same a...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.79.2.327
更新日期:1985-10-01 00:00:00
abstract::Ethylene supplied with indoleacetic acid at 0.1 and 1 mum inhibited elongation and enhanced swelling in epicotyls of decapitated and derooted pea seedlings (Pisum sativum L., var. Alaska). These growth responses were correlated with the development of cell walls rich in weak acid-extractable materials and pectic uroni...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.55.3.450
更新日期:1975-03-01 00:00:00
abstract::Phosphoenolpyruvate carboxylase purified from leaves of maize (Zea mays, L.) is sensitive to the presence of urea. Exposure to 2.5 m urea for 30 min completely inactivates the enzyme, whereas for a concentration of 1.5 m urea, about 1 h is required. Malate appears to have no effect on inactivation by urea of phosphoen...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.100.3.1366
更新日期:1992-11-01 00:00:00
abstract::Through a biochemical and a genetic approach, we have identified several plant genes encoding methylthioribose (MTR) kinase, an enzyme involved in recycling of methionine through the methylthioadenosine (MTA) cycle. OsMTK1, an MTR kinase from rice (Oryza sativa), is 48.6 kD in size and shows cooperative kinetics with ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.053587
更新日期:2004-12-01 00:00:00
abstract::The presence of two major aminopeptidases (aminopeptidases I and II) in the giant alga Chara australis was shown using polyacrylamide gel electrophoresis. Partially purified aminopeptidase I had a molecular weight of about 120,000, hydrolyzed both leucine-beta-naphthylamide (pH optimum 6.0) and alanine-beta-naphthylam...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.84.3.720
更新日期:1987-07-01 00:00:00
abstract::Mitogen-activated protein kinases (MAPKs) are fundamental components of the plant innate immune system. MPK3 and MPK6 are Arabidopsis (Arabidopsis thaliana) MAPKs activated by pathogens and elicitors such as oligogalacturonides (OGs), which function as damage-associated molecular patterns, and flg22, a well-known micr...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.111.174003
更新日期:2011-10-01 00:00:00
abstract::Ammonium (NH(4) (+)) nutrition inhibits water uptake and root exudation and decreases leaf water potential of tomato plants grown in solution culture. This inhibition is readily reversible by NO(3) (-) for short term exposures to NH(4) (+); however, recovery is delayed following long term exposures. ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.52.6.677
更新日期:1973-12-01 00:00:00
abstract::PIB-type ATPases transport heavy metal ions (Cu+, Cu2+, Zn2+, Cd2+, Co2+, etc.) across biological membranes. Several members of this subfamily are present in plants. Higher plants are the only eukaryotes where putative Zn(2+)-ATPases have been identified. We have cloned HMA2, a PIB-ATPase present in Arabidopsis (Arabi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.046292
更新日期:2004-11-01 00:00:00
abstract::Gravitropic asymmetry of auxin was observed in hypocotyls of tomato (Lycopersicon esculentum Mill.) soon after horizontal placement: the ratio of apically supplied [3H]IAA collected from the lower sides to that from the upper sides was about 1.4 between 5 and 10 minutes. This was adequately early to account for the ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.89.2.652
更新日期:1989-01-01 00:00:00
abstract::Peroxidase activity in the seed coats of soybean (Glycine max [L.] Merr.) is controlled by the Ep locus. We compared peroxidase activity in cell-free extracts from seed coat, root, and leaf tissues of three EpEp cultivars (Harosoy 63, Harovinton, and Coles) to three epep cultivars (Steele, Marathon, and Raiden). Extra...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.103.4.1061
更新日期:1993-12-01 00:00:00
abstract::The time course of abscisic acid (ABA) accumulation during water stress and of degradation following rehydration was investigated by analyzing the levels of ABA and its metabolites phaseic acid (PA) and alkalihydrolyzable conjugated ABA in excised leaf blades of Xanthium strumarium. Initial purification was by reverse...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.66.4.672
更新日期:1980-10-01 00:00:00
abstract::Gross O2 evolution and uptake by attached, drought-stressed leaves of wheat (Triticum aestivum) were measured using a 16O2/ 18O2 isotope technique and mass spectrometry. The activity of photosystem II, determined from the rate of 16O2 evolution, is only slightly affected under drought conditions. During drought stress...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.1.265
更新日期:1996-09-01 00:00:00
abstract::A novel alliinase (EC 4.4.1.4) was detected and purified from the roots of the Amazonian medicinal plant Petiveria alliacea. The isolated enzyme is a heteropentameric glycoprotein composed of two alpha-subunits (68.1 kD each), one beta-subunit (56.0 kD), one gamma-subunit (24.8 kD), and one delta-subunit (13.9 kD). Th...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.142430
更新日期:2009-11-01 00:00:00
abstract::Thermostability of the photosynthetic apparatus of abscisic acid (ABA)-treated seedlings of barley (Hordeum vulgare) was studied by light-scattering and by fluorescence measurements of isolated chloroplasts. ABA treatment markedly decreased heat damage of the chloroplast ultrastructure; an exogenous ABA concentration ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.98.4.1228
更新日期:1992-04-01 00:00:00
abstract::A barley (Hordeum vulgare L.) mutant, nar1a (formerly Az12), deficient in NADH nitrate reductase activity is, nevertheless, capable of growth with nitrate as the sole nitrogen source. In an attempt to identify the mechanism(s) of nitrate reduction in the mutant, nitrate reductase from nar1a was characterized to determ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.69.5.1200
更新日期:1982-05-01 00:00:00
abstract::Bicarbonate uptake by isolated chloroplast envelope membranes and intact chloroplasts of spinach (Spinacia oleracea L. var. Viroflay) in darkness exhibited a similar dependency upon temperature, pH, time, and concentrations of isolated or attached envelope membranes. This similarity in uptake properties demonstrates t...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.54.4.520
更新日期:1974-10-01 00:00:00
abstract::A serious limitation to the use of N(O,S)-heptafluorobutyryl isobutyl amino acid derivatives in the analysis of (15)N-labeling kinetics of amino acids in plant tissues, is that the amides glutamine and asparagine undergo acid hydrolysis to glutamate and aspartate, respectively, during derivatization. This led us to co...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.89.4.1161
更新日期:1989-04-01 00:00:00
abstract::Adenosine kinase (ADK) is a key enzyme that regulates intra- and extracellular levels of adenosine, thereby modulating methyltransferase reactions, production of polyamines and secondary compounds, and cell signaling in animals. Unfortunately, little is known about ADK's contribution to the regulation of plant growth ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.084798
更新日期:2006-10-01 00:00:00
abstract::Control of flowering and the regulation of plant architecture have been thoroughly investigated in a number of well-studied dicot plants such as Arabidopsis, Antirrhinum, and tobacco. However, in many important monocot seed crops, molecular information on plant reproduction is still limited. To investigate the regulat...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.125.3.1517
更新日期:2001-03-01 00:00:00
abstract::Nicotianamine (NA) occurs in all plants and chelates metal cations, including FeII, but reportedly not FeIII. However, a comparison of the FeII and ZnII affinity constants of NA and various FeIII-chelating aminocarboxylates suggested that NA should chelate FeIII. High-voltage electrophoresis of the FeNA complex formed...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.119.3.1107
更新日期:1999-03-01 00:00:00
abstract::A mutation in the ALTERED XYLOGLUCAN9 (AXY9) gene was found to be causative for the decreased xyloglucan acetylation phenotype of the axy9.1 mutant, which was identified in a forward genetic screen for Arabidopsis (Arabidopsis thaliana) mutants. The axy9.1 mutant also exhibits decreased O-acetylation of xylan, implyin...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.114.256479
更新日期:2015-04-01 00:00:00
abstract::The secretion of alpha-amylase from single isolated (Hordeum vulgare L. cv Himalaya) aleurone layers was studied in an automated flow-through apparatus. The apparatus, consisting of a modified sample analyzer linked to a chart recorder, automatically samples the flow-through medium at 1 minute intervals and assays for...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.70.4.1149
更新日期:1982-10-01 00:00:00
abstract::We have described the modulation of four auxin-regulated genes during the growth cycle of suspension-cultured tobacco (Nicotiana tabacum [L.] var White Burley) cells. The genes were transiently expressed 2 to 8 h after transfer of stationary phase cells to fresh medium, during the transition from the quiescent phase o...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.102.2.513
更新日期:1993-06-01 00:00:00
abstract::The physiological and molecular bases for contrasting [alpha]-amylase phenotypes were examined in germinating seeds of two barley (Hordeum vulgare L.) cultivars, Morex and Steptoe. Morex is a high-quality malting barley that develops high [alpha]-amylase activity soon after germination. Steptoe is a feed barley that d...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.102.1.195
更新日期:1993-05-01 00:00:00
abstract::Despite much study of the role of S-adenosylmethionine (SAM) in the methylation of DNA, RNA, and proteins, and as a cofactor for a wide range of biosynthetic processes, little is known concerning the intracellular transport of this essential metabolite. Screening of the Arabidopsis (Arabidopsis thaliana) genome yielde...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.086975
更新日期:2006-11-01 00:00:00
abstract::Explants of genetic tumors, tumors initiated by Agrobacterium tumefaciens strains B-6 and T-37, and excised pith plugs from Nicotiana glauca, N. langsdorffii, and N. glauca-langsdorffii were cultured on Murashige and Skoog medium. All cultures, pith callus and tumors with the exception of N. langsdorffii pith grew on ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.44.7.1073
更新日期:1969-07-01 00:00:00
abstract::Fresh fruit and vegetables are a major source of ascorbic acid (vitamin C), an important antioxidant for the human diet and also for plants. Ascorbic acid content in fruit exhibits a quantitative inheritance. Quantitative trait loci (QTL) for ascorbic acid content have been mapped in three tomato populations derived f...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.091413
更新日期:2007-04-01 00:00:00
abstract::The vacuoles of lower epidermal strips from Vicia faba exhibit an intrinsic green fluorescence when incubated in alkaline buffers. Using an alkaline-induced absorbance change as a spectrophotometric assay, the major pigment responsible for this fluorescence was isolated and identified as the flavonoid: kaempferol 3-O-...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.69.2.522
更新日期:1982-02-01 00:00:00