Assay of Proton-Coupled Glycolate and D-Glycerate Transport into Chloroplast Inner Envelope Membrane Vesicles by Stopped-Flow Fluorescence.

abstract：:Cytochrome P450s (P450s) constitute one of the major classes of enzymes that are responsible for detoxification of exogenous molecules both in animals and plants. On the basis of its inducibility by exogenous chemicals, we recently isolated a new plant P450, CYP76B1, from Jerusalem artichoke (Helianthus tuberosus) and...

journal_title：Plant physiology

authors： Robineau T,Batard Y,Nedelkina S,Cabello-Hurtado F,LeRet M,Sorokine O,Didierjean L,Werck-Reichhart D

更新日期：1998-11-01 00:00:00

abstract：:We functionally characterized the grape (Vitis vinifera) VvPIP2;4N (for Plasma membrane Intrinsic Protein) aquaporin gene. Expression of VvPIP2;4N in Xenopus laevis oocytes increased their swelling rate 54-fold. Northern blot and quantitative reverse transcription-polymerase chain reaction analyses showed that VvPIP2;...

journal_title：Plant physiology

authors： Perrone I,Gambino G,Chitarra W,Vitali M,Pagliarani C,Riccomagno N,Balestrini R,Kaldenhoff R,Uehlein N,Gribaudo I,Schubert A,Lovisolo C

更新日期：2012-10-01 00:00:00

abstract：:A method was developed where soybean seedlings were grown without roots to study the influence of hormones of root origin on shoot growth. Excision of the root resulted in inhibition of apical section growth and DNA synthesis and inhibited elongating section growth. A synthetic cytokinin restored DNA synthesis in the ...

journal_title：Plant physiology

authors： Holm RE,Key JL

更新日期：1969-09-01 00:00:00

abstract：:Transgenic tobacco plants carrying a fusion between the nopaline synthase (nos) promoter and chloramphenicol acetyltransferase (CAT) reporter gene (cat) were studied for their inducibility by salicylic acid (SA) or methyl jasmonate (MJ) treatments. Either chemical significantly increased CAT activity to a level much h...

journal_title：Plant physiology

authors： Kim SR,Kim Y,An G

更新日期：1993-09-01 00:00:00

abstract：:Rhythmic rotational movements with the midvein as the axis have been observed in the unifoliate leaves of Phaseolus angularis Wight grown under controlled environmental conditions with continuous light. The mean period of this movement for all leaves was 53.2 +/- 4.3 minutes and remained constant as the leaf matured, ...

journal_title：Plant physiology

authors： Alford DK,Tibbitts TW

更新日期：1971-01-01 00:00:00

abstract：:A new conjugate, 1-(gamma-L-glutamylamino)cyclopropane-1-carboxylic acid (GACC), of the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) is identified. The only previously identified conjugate of ACC is 1-(malonylamino)cyclopropane-1-carboxylic acid (MACC). GACC, not MACC, was the major conjugate formed ...

journal_title：Plant physiology

authors： Martin MN,Cohen JD,Saftner RA

更新日期：1995-11-01 00:00:00

abstract：:The course of senescence in the first leaves of light-grown Avena seedlings when attached to the plant has been compared with that previously studied in detached leaves and leaf segments. Proteolysis in the leaf, whether attached or detached, is accompanied by markedly polar basipetal transport of amino acids. This po...

journal_title：Plant physiology

authors： Thimann KV,Tetley RR,Van Thanh T

更新日期：1974-12-01 00:00:00

abstract：:Key aspects of leaf mitochondrial metabolism in the light remain unresolved. For example, there is debate about the relative importance of exporting reducing equivalents from mitochondria for the peroxisomal steps of photorespiration versus oxidation of NADH to generate ATP by oxidative phosphorylation. Here, we addre...

journal_title：Plant physiology

authors： Shameer S,Ratcliffe RG,Sweetlove LJ

更新日期：2019-08-01 00:00:00

abstract：:Polyacrylamide gel electrophoresis and electron microscopy revealed that accumulation of iron-protein in soybean nodules is influenced by nodule age, mutation in bradyrhizobia, and rhizobial/bradyrhizobial strain-soybean cultivar interactions. Iron-protein concentrations (micrograms per milligram protein) were inverse...

journal_title：Plant physiology

authors： Ko MP,Huang PY,Huang JS,Barker KR

更新日期：1987-02-01 00:00:00

abstract：:Phytoene synthase (PSY) catalyzes the highly regulated, frequently rate-limiting synthesis of the first biosynthetically formed carotene. While PSY constitutes a small gene family in most plant taxa, the Brassicaceae, including Arabidopsis (Arabidopsis thaliana), predominantly possess a single PSY gene. This monogenic...

journal_title：Plant physiology

authors： Álvarez D,Voß B,Maass D,Wüst F,Schaub P,Beyer P,Welsch R

更新日期：2016-12-01 00:00:00

abstract：:Membrane fractions from mature silver beet (Beta vulgaris) deveined leaf and leaf stem homogenates have associated Ca(2+) -dependent protein kinase. The Ca(2+) -dependent protein kinase activity is associated with plasma membranes (density 1.14-1.18 grams per cubic centimeter) as determined from copurification on isop...

journal_title：Plant physiology

authors： Klucis E,Polya GM

更新日期：1988-09-01 00:00:00

abstract：:The elongation rate of cowpea epicotyls from whole cowpea (Vigna sinensis) seedlings and derooted and debladed plants (explants) increased after the main light period (8-hour duration) was extended with either continuous low intensity tungsten light or brief (5 minutes) far-red (FR) irradiation. This end-of-day FR eff...

journal_title：Plant physiology

authors： García-Martínez JL,Keith B,Bonner BA,Stafford AE,Rappaport L

更新日期：1987-09-01 00:00:00

abstract：:The metabolism of labeled indole-3-acetic acid (IAA-2-(14)C) was investigated in Parthenocissus tricuspidata crown gall callus tissue. After 48 hours incubation, 85 to 90% of the supplied IAA was taken up by the tissue, and of that taken up, about 45% was conjugated with five amino acids. The conjugates found were asp...

journal_title：Plant physiology

authors： Feung CS,Hamilton RH,Mumma RO

更新日期：1976-11-01 00:00:00

abstract：:Kinetic characteristics of phosphoenolpyruvate carboxylase (PEPC) from the epiphytic C(3) or C(4): CAM intermediate plant, Peperomia camptotricha, were investigated. Few day versus night differences in V(max),K(m(PEP))), or malate inhibition were observed, even in extracts from water-stressed plants which characterist...

journal_title：Plant physiology

authors： Sipes DL,Ting IP

更新日期：1989-11-01 00:00:00

abstract：:This paper reports the purification and structural determination of the mannolipid shown previously (Ericson and Delmer 1977 Plant Physiol 59: 341-347) to serve as an intermediate in glycoprotein synthesis in cotyledons of Phaseolus vulgaris. The mannolipid was purified by chromatography in organic solvents on diethyl...

journal_title：Plant physiology

authors： Delmer DP,Kulow C,Ericson MC

更新日期：1978-01-01 00:00:00

abstract：:The sugars in the endosperm of a developing seed have many potential roles, including the supply of carbon to the developing embryo and controlling gene expression in it. Our understanding of their metabolism is, however, fragmentary and is confined to a very few species (especially Vicia spp.). To develop a quantitat...

journal_title：Plant physiology

authors： Hill LM,Morley-Smith ER,Rawsthorne S

更新日期：2003-01-01 00:00:00

abstract：:Crude aqueous extracts from Arabidopsis leaves were subjected to chromatographic separations, after which the different fractions were monitored for antimicrobial activity using the fungus Neurospora crassa as a test organism. Two major fractions were obtained that appeared to have the same abundance in leaves from un...

journal_title：Plant physiology

authors： Tierens KF,Thomma BP,Brouwer M,Schmidt J,Kistner K,Porzel A,Mauch-Mani B,Cammue BP,Broekaert WF

更新日期：2001-04-01 00:00:00

abstract：:Given the substantial changes in mitochondrial gene expression, the mitochondrial proteome, and respiratory function during rice (Oryza sativa) germination under anaerobic and aerobic conditions, we have attempted to identify changes in mitochondrial membrane transport capacity during these processes. We have assemble...

journal_title：Plant physiology

authors： Taylor NL,Howell KA,Heazlewood JL,Tan TY,Narsai R,Huang S,Whelan J,Millar AH

更新日期：2010-10-01 00:00:00

abstract：:Levels of gibberillins (GAs) and of abscisic acid (ABA) in attached leaves of romaine lettuce (Lactuca sativa L.) declined as the leaf became older. The time course of changes in hormone levels, determined in detached lettuce leaves kept in darkness, revealed that a sharp decline in GAs accompanied by a moderate rise ...

journal_title：Plant physiology

authors： Aharoni N

更新日期：1978-08-01 00:00:00

abstract：:Glyoxysomes isolated from castor bean (Ricinus communis L. var. zanzibariensis) endosperm have been stained by the cytochemical diaminobenzidine reaction. The reaction product obtained by preincubation with 3,3'-diaminobenzidine and incubation with the reagent and H(2)O(2) is distributed uniformly throughout the matri...

journal_title：Plant physiology

authors： Bieglmayer C,Ruis H

更新日期：1974-02-01 00:00:00

abstract：:The metabolism of d- and l-methionine by immature cotyledons of soybean (Glycine max, L. cv Elf) grown in culture has been investigated using solid-state (13)C and (15)N nuclear magnetic resonance. d-Methionine is taken up by the cotyledons and converted to an amide, most likely by N-malonylation. About 16% of the l-m...

journal_title：Plant physiology

authors： Coker GT,Garbow JR,Schaefer J

更新日期：1987-03-01 00:00:00

abstract：:Autoradiographs of (22)Na-loaded bean (Phaseolus vulgaris L. cv. ;Brittle Wax') leaf slices showed that most of the tracer was concentrated in vascular tissue. Rubidium-86 was uniformly distributed in slices that had been incubated in darkness; after incubation in the light many small dark spots appeared on the autora...

journal_title：Plant physiology

authors： Jacoby B

更新日期：1975-06-01 00:00:00

abstract：:In wheat (Triticum aestivum) seedlings subjected to a mild water stress (water potential of -0.3 MPa), the leaf-elongation rate was reduced by one-half and the mitotic activity of mesophyll cells was reduced to 42% of well-watered controls within 1 d. There was also a reduction in the length of the zone of mesophyll c...

journal_title：Plant physiology

authors： Schuppler U,He PH,John PC,Munns R

更新日期：1998-06-01 00:00:00

abstract：:We understand few details about how the arrangement and interactions of cell wall polymers produce the mechanical properties of primary cell walls. Consequently, we cannot quantitatively assess if proposed wall structures are mechanically reasonable or assess the effectiveness of proposed mechanisms to change mechanic...

journal_title：Plant physiology

authors： Kha H,Tuble SC,Kalyanasundaram S,Williamson RE

更新日期：2010-02-01 00:00:00

abstract：:Plant growth and development are strongly dependent on sink-source interactions. In the majority of plants, sucrose (Suc) is the dominant form in which photo-assimilate is transported from source to sinks. Although the effects of Suc on photosynthetic metabolism have been intensively studied, the effect of Suc supply ...

journal_title：Plant physiology

authors： Roessner-Tunali U,Urbanczyk-Wochniak E,Czechowski T,Kolbe A,Willmitzer L,Fernie AR

更新日期：2003-10-01 00:00:00

abstract：:In characterizing the enzymes involved in the formation of very long-chain fatty acids (VLCFAs) in the Brassicaceae, we have generated a series of mutants of Arabidopsis thaliana that have reduced VLCFA content. Here we report the characterization of a seed lipid mutant, AS11, which, in comparison to wild type (WT), h...

journal_title：Plant physiology

authors： Katavic V,Reed DW,Taylor DC,Giblin EM,Barton DL,Zou J,Mackenzie SL,Covello PS,Kunst L

更新日期：1995-05-01 00:00:00

abstract：:A gene (EGL1) encoding an endo-beta-1,4-D-glucanase (EGase, EC 3.2.1.4) of pea (Pisum sativum) has been cloned and characterized. EGL1 encodes a 486-amino acid polypeptide, including a 24-mer putative signal peptide. The mature protein has a calculated molecular mass of 51.3 kD and an isoelectric point of 9.1. This pe...

journal_title：Plant physiology

authors： Wu SC,Blumer JM,Darvill AG,Albersheim P

更新日期：1996-01-01 00:00:00

abstract：:ASSIMILATORY NITRATE REDUCTASE (NADH: nitrate oxidoreductase, EC 1.6.6.1) from Chlorella vulgaris has been purified to electrophoretic homogeneity with an overall yield of 60% by a procedure that utilizes blue dextran-agarose as an affinity column. Nitrate reductase binds to blue dextran and is not eluted in the prese...

journal_title：Plant physiology

authors： Solomonson LP

更新日期：1975-12-01 00:00:00

abstract：:Experiments were conducted in growth chambers to examine the effect of a mild water stress (-200 kilopascals polyethylene glycol) on frond elongation and water status of the ostrich fern (Matteuccia struthiopteris [L.] Todaro). Measurements were taken for two days, starting one day after the application of polyethylen...

journal_title：Plant physiology

authors： Prange RK,Ormrod DP

更新日期：1983-05-01 00:00:00

abstract：:We have tested directly the effect of Helminthosporium maydis T (Hmt) toxin and various analogs on the membrane potential formed in mitochondria isolated from a Texas (T) cytoplasmic male-sterile and a normal (N) corn. ATP, malate or succinate generated a membrane potential (negative inside) as monitored by the absorb...

journal_title：Plant physiology

authors： Holden MJ,Sze H

更新日期：1987-07-01 00:00:00