Abstract:
:Cytochrome P450s (P450s) constitute one of the major classes of enzymes that are responsible for detoxification of exogenous molecules both in animals and plants. On the basis of its inducibility by exogenous chemicals, we recently isolated a new plant P450, CYP76B1, from Jerusalem artichoke (Helianthus tuberosus) and showed that it was capable of dealkylating a model xenobiotic compound, 7-ethoxycoumarin. In the present paper we show that CYP76B1 is more strongly induced by foreign compounds than other P450s isolated from the same plant, and metabolizes with high efficiency a wide range of xenobiotics, including alkoxycoumarins, alkoxyresorufins, and several herbicides of the class of phenylureas. CYP76B1 catalyzes the double N-dealkylation of phenylureas with turnover rates comparable to those reported for physiological substrates and produces nonphytotoxic compounds. Potential uses for CYP76B1 thus include control of herbicide tolerance and selectivity, as well as soil and groundwater bioremediation.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Robineau T,Batard Y,Nedelkina S,Cabello-Hurtado F,LeRet M,Sorokine O,Didierjean L,Werck-Reichhart Ddoi
10.1104/pp.118.3.1049subject
Has Abstractpub_date
1998-11-01 00:00:00pages
1049-56issue
3eissn
0032-0889issn
1532-2548journal_volume
118pub_type
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