Abstract:
:Photosynthetic carbon metabolism was characterized in four photoautotrophic cell suspension cultures. There was no apparent difference between two soybean (Glycine max) and one cotton (Gossypium hirsutum) cell line which required 5% CO(2) for growth, and a unique cotton cell line that grows at ambient CO(2) (660 microliters per liter). Photosynthetic characteristics in all four lines were more like C(3) mesophyll leaf cells than the cell suspension cultures previously studied. The pattern of (14)C-labeling reflected the high ratio of ribulosebisphosphate carboxylase to phosphoenolpyruvate carboxylase activity and showed that CO(2) fixation occurred primarily by the C(3) pathway. Photorespiration occurred at 330 microliters per liter CO(2), 21% O(2) as indicated by the synthesis of high levels of (14)C-labeled glycine and serine in a pulse-chase experiment and by oxygen inhibition of CO(2) fixation. Short-term CO(2) fixation in the presence and absence of carbonic anhydrase showed CO(2), not HCO(3) (-), to be the main source of inorganic carbon taken up by the low CO(2)-requiring cotton cells. The cells did not have a CO(2)-concentrating mechanism as indicated by silicone oil centrifugation experiments. Carbonic anhydrase was absent in the low CO(2)-requiring cotton cells, present in the high CO(2)-requiring soybean cell lines, and absent in other high CO(2) cell lines examined. Thus, the presence of carbonic anhydrase is not an essential requirement for photoautotrophy in cell suspension cultures which grow at either high or low CO(2) concentrations.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Roeske CA,Widholm JM,Ogren WLdoi
10.1104/pp.91.4.1512subject
Has Abstractpub_date
1989-12-01 00:00:00pages
1512-9issue
4eissn
0032-0889issn
1532-2548journal_volume
91pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::We have analyzed gene regulation of the Lhc supergene family in poplar (Populus spp.) and Arabidopsis (Arabidopsis thaliana) using digital expression profiling. Multivariate analysis of the tissue-specific, environmental, and developmental Lhc expression patterns in Arabidopsis and poplar was employed to characterize ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.073304
更新日期:2006-03-01 00:00:00
abstract::Piperonylic acid (PA) is a natural molecule bearing a methylenedioxy function that closely mimics the structure of trans-cinnamic acid. The CYP73A subfamily of plant P450s catalyzes trans-cinnamic acid 4-hydroxylation, the second step of the general phenylpropanoid pathway. We show that when incubated in vitro with ye...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.118.1.209
更新日期:1998-09-01 00:00:00
abstract::Three aspartate aminotransferase isoenzymes were identified from extracts of carrot (Daucus carota L.) cell suspension cultures. These isoenzymes were separated by DEAE chromatography and were analyzed on native gradient polyacrylamide gels. The relative molecular weights of the isoenzymes were 111,000 +/- 5000, 105,0...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.92.3.587
更新日期:1990-03-01 00:00:00
abstract::Two-dimensional electrophoresis was performed on proteins of bundle sheath and mesophyll cells isolated from the C(4) grass Digitaria sanguinalis (L.) Scop. Two-dimensional maps of these proteins were constructed and ribulose-1,5-biphosphate carboxylase and phosphoenolpyruvate carboxylase were identified. Of the total...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.70.5.1359
更新日期:1982-11-01 00:00:00
abstract::Acetyl-coenzyme A (acetyl-CoA) formed within the plastid is the precursor for the biosynthesis of fatty acids and, through them, a range of important biomolecules. The source of acetyl-CoA in the plastid is not known, but two enzymes are thought to be involved: acetyl-CoA synthetase and plastidic pyruvate dehydrogenas...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.123.2.497
更新日期:2000-06-01 00:00:00
abstract::As regulators of gene expression in multicellular organisms, microRNAs (miRNAs) are crucial for growth and development. Although a plethora of factors involved in their biogenesis and action in Arabidopsis (Arabidopsis thaliana) has been described, these processes and their fine-tuning are not fully understood. Here, ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.17.01313
更新日期:2018-01-01 00:00:00
abstract::Five recombinant inbred lines (RILs) of Arabidopsis (Arabidopsis thaliana), previously selected from the Bay-0 x Shahdara RIL population on the basis of differential leaf senescence phenotypes (from early senescing to late senescing) when cultivated under nitrogen (N)-limiting conditions, were analyzed to monitor meta...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.119040
更新日期:2008-07-01 00:00:00
abstract::In many flowering plants, xyloglucan is a major component of primary cell walls, where it plays an important role in growth regulation. Xyloglucan can be degraded by a suite of exoglycosidases that remove specific sugars. In this work, we show that the xyloglucan backbone, formed by (1→4)-linked β-d-glucopyranosyl res...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.16.01713
更新日期:2017-02-01 00:00:00
abstract::Vanadate (Na(3)VO(4)) selectively and reversibly affects the rhythmic movement of Albizzia julibrissin leaflets. Leaflets floated on 1 millimolar vanadate open at the same rate or more rapidly than controls, but closure is inhibited. After 6 to 24 hours incubation, the inhibition can be reversed by a 24-to 48-hour per...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.64.5.905
更新日期:1979-11-01 00:00:00
abstract::PHOSPHATE STARVATION RESPONSE1 (PHR1) is a key regulatory component of the response to phosphate (Pi) starvation. However, the regulation of PHR1 in this response remains poorly understood. Here, we report that PHR1 is a target of the transcription factors AUXIN RESPONSE FACTOR7 (ARF7) and ARF19 and is positively regu...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.17.01713
更新日期:2018-09-01 00:00:00
abstract::Polysaccharide analyses of mutants link several of the glycosyltransferases encoded by the 10 CesA genes of Arabidopsis to cellulose synthesis. Features of those mutant phenotypes point to particular genes depositing cellulose predominantly in either primary or secondary walls. We used transformation with antisense co...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.010931
更新日期:2002-06-01 00:00:00
abstract::The cellular import of the hormone auxin is a fundamental requirement for the generation of auxin gradients that control a multitude of plant developmental processes. The AUX/LAX family of auxin importers, exemplified by AUX1 from Arabidopsis (Arabidopsis thaliana), has been shown to mediate auxin import when expresse...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.122044
更新日期:2008-09-01 00:00:00
abstract::The flow of sap in Populus tremuloides was studied by the thermoelectric method immediately prior to and at several periods of time after radical girdling. Similarly, the flow of sap in Betula papyrifera was studied before and after girdling or injection with metabolic inhibitors or a filtrate of a culture of Ceratocy...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.41.4.641
更新日期:1966-04-01 00:00:00
abstract::Two mutations, amylose-extender and waxy, which affect the proportion of amylose and amylopectin of starch synthesized in the endosperm of maize (Zea mays L.) seeds, are also expressed in the pollen. However, most mutations that affect starch synthesis in the maize endosperm are not expressed in the pollen. In an atte...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.63.2.312
更新日期:1979-02-01 00:00:00
abstract::Root branching is critical for plants to secure anchorage and ensure the supply of water, minerals, and nutrients. To date, research on root branching has focused on lateral root development in young seedlings. However, many other programs of postembryonic root organogenesis exist in angiosperms. In cereal crops, the ...
journal_title:Plant physiology
pub_type: 杂志文章,评审
doi:10.1104/pp.114.245423
更新日期:2014-10-01 00:00:00
abstract::Eukaryotic Δ6-desaturases are microsomal enzymes that balance the synthesis of ω-3 and ω-6 C18-polyunsaturated fatty acids (C18-PUFAs) according to their specificity. In several microalgae, including Ostreococcus tauri, plastidic C18-PUFAs are strictly regulated by environmental cues suggesting an autonomous control o...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.20.00281
更新日期:2020-09-01 00:00:00
abstract::A mutant of Arabidopsis thaliana has been isolated in which ribulose-1,5-bisphosphate carboxylase is present in a nonactivatable form in vivo. The mutation appears to affect carboxylase activation specifically, and not any other enzyme of the photosynthesis or photorespiratory cycles. The effect of the mutation on car...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.70.2.381
更新日期:1982-08-01 00:00:00
abstract::Plant viruses utilize the vascular system for systemic movement. The plant vascular network also transports water, photosynthates, and signaling molecules and is essential for plant growth. However, the molecular mechanisms governing vascular development and patterning are still largely unknown. From viral transport s...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.083519
更新日期:2006-10-01 00:00:00
abstract::Mitochondria isolated from Kalanchoë daigremontiana, a Crassulacean acid metabolism plant, decarboxylate added malate to pyruvate at rates of up to 100 micromoles per hour per milligram original chlorophyll in the presence of ADP. Omitting ADP reduces this rate by approximately 50%. Antimycin A inhibits malate decarbo...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.65.4.675
更新日期:1980-04-01 00:00:00
abstract::Acetophenones are phenolic compounds involved in the resistance of white spruce (Picea glauca) against spruce budworm (Choristoneura fumiferiana), a major forest pest in North America. The acetophenones pungenol and piceol commonly accumulate in spruce foliage in the form of the corresponding glycosides, pungenin and ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.17.00611
更新日期:2017-10-01 00:00:00
abstract::The essentiality of roots to the short-term responses of leaf elongation to salinity was tested by removing the roots of maize (Zea mays L.) from the shoots and comparing the initial short-term response of leaf elongation to that with intact plants. Eightday-old seedlings growing in solution culture were treated with ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.95.3.965
更新日期:1991-03-01 00:00:00
abstract::We present immunocytochemical localizations of four enzymes involved in p-menthane monoterpene biosynthesis in mint: the large and small subunits of peppermint (Mentha x piperita) geranyl diphosphate synthase, spearmint (Mentha spicata) (-)-(4S)-limonene-6-hydroxylase, peppermint (-)-trans-isopiperitenol dehydrogenase...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.050229
更新日期:2004-12-01 00:00:00
abstract::A number of plants have been surveyed with respect to isolation by mild grinding in large quantities of leaf cells. The extent of recovery of mesophyll cells per unit leaf area was found to vary with plant species and the method of grinding. Greater than 70% recovery was obtained from the leaves of Canna indica L., Cr...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.54.4.569
更新日期:1974-10-01 00:00:00
abstract::Enriched mitochondrial fractions isolated from durum wheat seedlings via differential centrifugation exhibited classical cyanide- or antimycin A-insensitive O(2) uptake which was inhibited by either salicylhydroxamic acid or propyl gallate. Further purification of this fraction using Percoll density gradients resulted...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.67.3.594
更新日期:1981-03-01 00:00:00
abstract::The influence of light or darkness on stomatal opening in epidermal strips of Commelina benghalensis was evaluated in the presence or absence of O(2) and/or metabolic inhibitors. Opening was restricted in nitrogen and was promoted by NADH and acids of the tricarboxylic acid cycle (succinate and alpha-ketoglutarate) in...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.67.2.385
更新日期:1981-02-01 00:00:00
abstract::The starch-granule-bound starch synthase from Zea mays has been solubilized with a recovery of between 50 and 84%. Chromatography of the solubilized enzyme on DEAE-Sepharose resolves two fractions of activity which may be distinguished by their response to citrate. Neither solubilized isoenzyme displays any significan...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.73.1.175
更新日期:1983-09-01 00:00:00
abstract::Salinity is an environmental stress that causes decline in crop yield. Avicennia officinalis and other mangroves have adaptations such as ultrafiltration at the roots aided by apoplastic cell wall barriers to thrive in saline conditions. We studied a cytochrome P450 gene from A. officinalis, AoCYP94B1, and its putativ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.20.01054
更新日期:2020-12-01 00:00:00
abstract::The interaction of tropisms is important in determining the final growth form of the plant body. In roots, gravitropism is the predominant tropistic response, but phototropism also plays a role in the oriented growth of roots in flowering plants. In blue or white light, roots exhibit negative phototropism that is medi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.013847
更新日期:2003-03-01 00:00:00
abstract::Superoxide dismutases (SODs) are ubiquitous metalloenzymes that catalyze the dismutation of superoxide radicals. Chloroplasts have two isozymes, copper/zinc SOD (Cu/ZnSOD) and iron SOD (FeSOD), encoded by nuclear genes. Because bryophytes are considered as the earliest land plants, they are one of the most interesting...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.107.114868
更新日期:2008-04-01 00:00:00
abstract::Arbuscular mycorrhiza (AM) is a widespread symbiotic association between plants and fungal microsymbionts that supports plant development under nutrient-limiting and various stress conditions. In this study, we focused on the overlapping genetic program activated by two commonly studied microsymbionts in addition to i...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.056572
更新日期:2005-04-01 00:00:00