Abstract:
:A gene (EGL1) encoding an endo-beta-1,4-D-glucanase (EGase, EC 3.2.1.4) of pea (Pisum sativum) has been cloned and characterized. EGL1 encodes a 486-amino acid polypeptide, including a 24-mer putative signal peptide. The mature protein has a calculated molecular mass of 51.3 kD and an isoelectric point of 9.1. This pea EGase shares significant similarity with EGases from other plant species, but it appears to be distinct from the EGases associated with abscission and fruit ripening. Although EGL1 transcripts are detected in all parts of pea plants, they are relatively abundant in flowers and young pods undergoing rapid growth and most abundant in elongating epicotyls of etiolated seedlings. When epicotyl segments (6 mm long, 4 mm from the apical hook) are incubated in a 5 microM solution of the synthetic auxin analog 2,4-dichlorophenoxyacetic acid, the concentration of EGL1 mRNA increases about 10-fold when the segments elongate most rapidly.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Wu SC,Blumer JM,Darvill AG,Albersheim Pdoi
10.1104/pp.110.1.163subject
Has Abstractpub_date
1996-01-01 00:00:00pages
163-70issue
1eissn
0032-0889issn
1532-2548pii
110/1/163journal_volume
110pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::N redistribution patterns and the N composition of vegetative tissues above the peduncle node of wheat (Triticum aestivum L.) plants with altered reproductive sink strength were evaluated to determine the role of vegetative storage proteins in the temporary storage of excess N destined for export. The degree of leaf s...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.99.4.1469
更新日期:1992-08-01 00:00:00
abstract::The elongation rate of cowpea epicotyls from whole cowpea (Vigna sinensis) seedlings and derooted and debladed plants (explants) increased after the main light period (8-hour duration) was extended with either continuous low intensity tungsten light or brief (5 minutes) far-red (FR) irradiation. This end-of-day FR eff...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.85.1.212
更新日期:1987-09-01 00:00:00
abstract::Increasing pea (Pisum sativum) seed nutritional value and particularly seed protein content, while maintaining yield, is an important challenge for further development of this crop. Seed protein content and yield are complex and unstable traits, integrating all the processes occurring during the plant life cycle. Duri...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.107.096966
更新日期:2007-06-01 00:00:00
abstract::A controlled flow of porphyrin metabolites is critical for organisms, but little is known about the control of porphyrin biosynthesis under environmental stress. We monitored transgenic rice (Oryza sativa) plants expressing Myxococcus xanthus protoporphyrinogen oxidase (PPO) for their response to drought stress. Trans...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.111.188276
更新日期:2011-12-01 00:00:00
abstract::A chlorophyll (Chl)-protein complex designated CPVI-4 becomes the major pigment-protein complex in Synechococcus sp. PCC7942 cells grown under conditions of iron limitation. Work by Laudenbach et al. (J Bacteriol [1988] 170: 5018-5026) has identified an iron-repressible operon, designated isiAB, containing the flavodo...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.103.3.893
更新日期:1993-11-01 00:00:00
abstract::Dunaliella bardawil, a beta-carotene-accumulating halotolerant alga, has been analyzed for the effect of various growth conditions on its pigment content, and compared with Dunaliella salina, a beta-carotene nonaccumulating species. In D. bardawil, increasing light intensity and light period or inhibiting growth by va...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.72.3.593
更新日期:1983-07-01 00:00:00
abstract::We describe the development and utilization of a new imaging technology for plant biology, optical coherence microscopy (OCM), which allows true in vivo visualization of plants and plant cells. This novel technology allows the direct, in situ (e.g. plants in soil), three-dimensional visualization of cells and events i...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.123.1.3
更新日期:2000-05-01 00:00:00
abstract::Promoters of most seed proteins in legumes contain one or more 5'-CATGCAT-3' elements. To test if these elements have a function in the expression of these genes, the 2.3 kilobase pairs Gy2 glycinin promoter was ligated to a beta-glucuronidase reporter sequence and transformed into tobacco. Elimination of a 5'-CATGCAT...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.98.1.387
更新日期:1992-01-01 00:00:00
abstract::The relative effects of water stress on growth parameters of subterranean clover (Trifolium subterraneum L. cv. Woogenellup) dependent on either N(2) or 8 millimolar NH(4)NO(3) for N were examined. Whole-plant carbon exchange rate (CER), acetylene reduction (AR), dry matter production, and Kjeldahl N accumulation were...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.69.2.416
更新日期:1982-02-01 00:00:00
abstract::Recent structure-function analysis of heterologously expressed K+-selective inward-rectifying channels (KIRCs) from plants has revealed that external protons can have opposite effects on different members of the same gene family. An important question is how the diverse response of KIRCs to apoplastic pH is reflected ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.120.1.331
更新日期:1999-05-01 00:00:00
abstract::Nitrogen (N) is an essential requirement for kernel growth in maize (Zea mays); however, little is known about how N assimilates are metabolized in young earshoots during seed development. The objective of this study was to assess amino acid metabolism in cob and spikelet tissues during the critical 2 weeks following ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.043778
更新日期:2004-12-01 00:00:00
abstract::The infectivity of Agrobacterium tumefaciens strain B6 was inhibited about 50% when these bacteria were inoculated on potato discs with equal viable cell counts of a weakly virulent strain of A. tumefaciens (B-48) or autoclaved strains of B6 or B-48. Inhibition by B-48 or autoclaved B6 could still be obtained when the...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.61.6.1031
更新日期:1978-06-01 00:00:00
abstract::Cotyledon explants from germinated 1-day-old soybean seedling were inoculated with single or mixed strains of Agrobacterium tumefaciens. Mixed-strain infections with the supervirulent L,L-succinamopine type strain A281 (pTiBo542) and strain LBA4404 carrying an octopine type virulence (vir) region and a binary vector (...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.88.3.570
更新日期:1988-11-01 00:00:00
abstract::Levels of ethylene biosynthesis and ethylene-inducible gene expression in wild-type tomato (Lycopersicon esculentum) fruit and in nonripening fruit from the tomato mutant rin (ripening inhibitor) were compared in order to investigate the mechanism of ethylene action. Whereas wild-type tomato fruit dramatically increas...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.88.2.370
更新日期:1988-10-01 00:00:00
abstract::Primary leaves of 8-day-old Phaseolus vulgaris plants dipped in water at 46.5 to 47.5 C for 2 minutes were found to have decreased synthesis of lipid and beta-glucan compared to controls; UDP-glucose was used as substrate by cell-free preparations. As time elapsed, leaf growth, which was inhibited within the first 12 ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.53.1.118
更新日期:1974-01-01 00:00:00
abstract::We examined the consistency of the native diploid Nicotiana attenuata (Na)'s herbivore-induced transcriptional changes in the two allotetraploid natives, Nicotiana clevelandii (Nc) and Nicotiana quadrivalvis (Nq), which are thought to be derived from hybridizations with an ancestral Na. An analysis of nuclear-encoded ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.103.037036
更新日期:2004-05-01 00:00:00
abstract::The LOSS OF APOMEIOSIS (LOA) locus is one of two dominant loci known to control apomixis in the eudicot Hieracium praealtum. LOA stimulates the differentiation of somatic aposporous initial cells after the initiation of meiosis in ovules. Aposporous initial cells undergo nuclear proliferation close to sexual megaspore...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.111.181164
更新日期:2011-11-01 00:00:00
abstract::The presence of two major aminopeptidases (aminopeptidases I and II) in the giant alga Chara australis was shown using polyacrylamide gel electrophoresis. Partially purified aminopeptidase I had a molecular weight of about 120,000, hydrolyzed both leucine-beta-naphthylamide (pH optimum 6.0) and alanine-beta-naphthylam...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.84.3.720
更新日期:1987-07-01 00:00:00
abstract::The rates of oxidation of ent-kaur-16-ene to ent-kaur-16-en-19-ol, ent-kaur-16-en-19-al, ent-kaur-16-en-19-oic acid, and ent-kaur-16-en-7alpha-ol-19-oic acid are maximal in microsomes prepared from the endosperm of immature Marah macrocarpus seeds in which the cotyledons are approximately one-half the overall length o...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.58.4.473
更新日期:1976-10-01 00:00:00
abstract::Fluence-response curves for the induction of seed germination after 24 hours pretreatment at 35 degrees C of Rumex obtusifolius and Arabidopsis thaliana show two phases of response: (a) a very low fluence-response (10(-4) - 10(-1) micromoles per square meter) and (b) a low fluence-response (1 - 10(3) micromoles per sq...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.79.1.299
更新日期:1985-09-01 00:00:00
abstract::To dissect the expression of the psbB gene cluster of the Chlamydomonas reinhardtii chloroplast genome and to assess the role of the photosystem II H-phosphoprotein (PSII-H) in the biogenesis and/or stabilization of PSII, an aadA gene cassette conferring spectinomycin resistance was employed for mutagenesis. Disruptio...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.4.1359
更新日期:1997-04-01 00:00:00
abstract::Rates of assimilation and transpiration in Eucalyptus pauciflora Sieb. ex Spreng were measured at various ambient partial pressures of CO(2) and various irradiances and were used to estimate leaf conductance and intercellular partial pressure of CO(2). The responses of leaf conductance and rate of assimilation to chan...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.62.4.670
更新日期:1978-10-01 00:00:00
abstract::The relationship between gravitropism and surface electrical potentials was studied using etiolated epicotyls of adzuki bean (Phaseolus angularis). Early downward curvature (or transient positive gravitropic response) was observed about 1 min after gravistimulation. The downward curvature was closely related to the sp...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.3.875
更新日期:1994-07-01 00:00:00
abstract::Plasma membrane-resident receptor kinases (RKs) initiate signaling pathways important for plant immunity and development. In Arabidopsis (Arabidopsis thaliana), the receptor for the elicitor-active peptide epitope of bacterial flagellin, flg22, is encoded by FLAGELLIN SENSING2 (FLS2), which promotes plant immunity. De...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.111.192575
更新日期:2012-06-01 00:00:00
abstract::Glutamine synthetase (GS) from Synechocystis sp. PCC 6803 was inactivated in vivo by transferring cells from light to darkness or by incubation with the photosynthetic inhibitor 3-(3,4-dichlorophenyl)-1,1-dimethylurea but not with 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone. Addition of glucose prevented both dark...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.3.899
更新日期:1995-11-01 00:00:00
abstract::Glutathione is generally accepted as the principal electron donor for dehydroascorbate (DHA) reduction. Moreover, both glutathione and DHA affect cell cycle progression in plant cells. But other mechanisms for DHA reduction have been proposed. To investigate the connection between DHA and glutathione, we have evaluate...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.103.033548
更新日期:2004-04-01 00:00:00
abstract::To study in detail the hypersensitive reaction, one of the major defense responses of plants against microbial infection, we used a model system of reduced complexity with cultured parsley (Petroselinum crispum) cells infected with the phytopathogenic fungus Phytophthora infestans. Experimental conditions were establi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.1.433
更新日期:1996-09-01 00:00:00
abstract::When soybean Glycine max var Wayne seedlings are shifted from a normal growth temperature of 28 degrees C up to 40 degrees C (heat shock or HS), there is a dramatic change in protein synthesis. A new set of proteins known as heat shock proteins (HSPs) is produced and normal protein synthesis is greatly reduced. A brie...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.74.1.152
更新日期:1984-01-01 00:00:00
abstract::The activity of acifluorfen-methyl (AFM); methyl 5-(2-chloro-4-[trifluoromethyl] phenoxy)-2-nitrobenzoate in excised cucumber cotyledons (Cucumis sativus L.) was examined. AFM induced membrane disruption, was significantly greater when etiolated cotyledons were illuminated 16 hours at 150 microeinsteins per square met...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.84.4.1114
更新日期:1987-08-01 00:00:00
abstract::Plants have evolved complicated regulatory systems to control immune responses. Both positive and negative signaling pathways interplay to coordinate development of a resistance response with the appropriate amplitude and duration. AtNUDT7, a Nudix domain-containing protein in Arabidopsis (Arabidopsis thaliana) that h...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.107.103374
更新日期:2007-09-01 00:00:00