Abstract:
:The spontaneous occurrence of resistance to the herbicide glyphosate in weed species has been an extremely infrequent event, despite over 20 years of extensive use. Recently, a glyphosate-resistant biotype of goosegrass (Eleusine indica) was identified in Malaysia exhibiting an LD(50) value approximately 2- to 4-fold greater than the sensitive biotype collected from the same region. A comparison of the inhibition of 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) activity by glyphosate in extracts prepared from the resistant (R) and sensitive (S) biotypes revealed an approximately 5-fold higher IC(50)(glyphosate) for the (R) biotype. Sequence comparisons of the predicted EPSPS mature protein coding regions from both biotypes revealed four single-nucleotide differences, two of which result in amino acid changes. One of these changes, a proline to serine substitution at position 106 in the (R) biotype, corresponds to a substitution previously identified in a glyphosate-insensitive EPSPS enzyme from Salmonella typhimurium. Kinetic data generated for the recombinant enzymes suggests that the second substitution identified in the (R) EPSPS does not contribute significantly to its reduced glyphosate sensitivity. Escherichia coli aroA- (EPSPS deficient) strains expressing the mature EPSPS enzyme from the (R) biotype exhibited an approximately 3-fold increase in glyphosate tolerance relative to strains expressing the mature EPSPS from the (S) biotype. These results provide the first evidence for an altered EPSPS enzyme as an underlying component of evolved glyphosate resistance in any plant species.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Baerson SR,Rodriguez DJ,Tran M,Feng Y,Biest NA,Dill GMdoi
10.1104/pp.001560subject
Has Abstractpub_date
2002-07-01 00:00:00pages
1265-75issue
3eissn
0032-0889issn
1532-2548journal_volume
129pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::Allantoinase catalyzes the hydrolysis of allantoin to allantoic acid, a reaction important in both biogenesis and degradation of ureides. Ureide production in cotyledons of germinating soybean (Glycine max L.) seeds has not been studied extensively but may be important in mobilizing nitrogen reserves. Allantoinase was...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.103.4.1235
更新日期:1993-12-01 00:00:00
abstract::Ethylene evolved from monoecious and gynoecious cucumber (Cucumis sativus) plants grown under short and long day conditions was determined. More ethylene was evolved from floral buds and apices bearing buds than from whole seedlings of comparable weight. More ethylene also was evolved from apices of the gynoecious tha...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.49.6.998
更新日期:1972-06-01 00:00:00
abstract::Plants possess many metabolic genes for the production of a wide variety of phytochemicals in a tissue-specific manner. However, the metabolic systems behind the diversity and tissue-dependent regulation still remain unknown due to incomplete characterization of phytochemicals produced in a single plant species. Thus,...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.148031
更新日期:2010-02-01 00:00:00
abstract::The metabolism of soluble nucleotides was investigated in wheat (Triticum aestivum var. Olympic) aleurone layers treated with gibberellic acid. Whereas nucleotide levels were relatively unaffected by the treatment, a transient increase was observed in the incorporation of (32)P. The effect was maximal 30 to 60 minutes...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.49.3.404
更新日期:1972-03-01 00:00:00
abstract::Using a genetic approach, we have identified and characterized a novel protein, named Msf1 (Maintenance factor for photosystem I), that is required for the maintenance of specific components of the photosynthetic apparatus in the green alga Chlamydomonas reinhardtii Msf1 belongs to the superfamily of light-harvesting ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.16.01465
更新日期:2017-08-01 00:00:00
abstract::Amyloplasts of starchy tissues such as those of maize (Zea mays L.) function in the synthesis and accumulation of starch during kernel development. ADP-glucose pyrophosphorylase (AGPase) is known to be located in chloroplasts, and for many years it was generally accepted that AGPase was also localized in amyloplasts o...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.117.4.1235
更新日期:1998-08-01 00:00:00
abstract::The plant cuticle is an extracellular hydrophobic layer that covers the aerial epidermis of all land plants, providing protection against desiccation and external environmental stresses. The past decade has seen considerable progress in assembling models for the biosynthesis of its two major components, the polymer cu...
journal_title:Plant physiology
pub_type: 杂志文章,评审
doi:10.1104/pp.113.222737
更新日期:2013-09-01 00:00:00
abstract::Ca-mediated processes are known to be involved in transducing many developmental, hormonal, and environmental cues in plant cells. In this study, the role of Ca in the perception of anoxic stress signals by maize (Zea mays L. cv B73) roots was assessed by studying the effect of various Ca antagonists on the induction ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.1.369
更新日期:1994-05-01 00:00:00
abstract::Long term feeding of acetate-2-(14)C, (14)CO(2), citrate-1,5-(14)C, fumarate-2,3-(14)C, and succinate-2,3-(14)C to mung bean (Phaseolus aureus L. var. Mungo) leaves in the dark gave labeling predominantly in tricarboxylic acid cycle intermediates. Kinetics of the intermediates during dark/light/dark transitions showed...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.53.6.886
更新日期:1974-06-01 00:00:00
abstract::Cotyledons excised from dark-grown cucumber (Cucumis sativus L. cv. Aonagajibai) seedlings were incubated in the dark with the cytokinin benzyladenine for different time periods. Then, various greening parameters were examined, including protochlorophyll(ide) to chlorophyll(ide) photoconversion and delta-aminolevulini...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.69.3.663
更新日期:1982-03-01 00:00:00
abstract::Respiratory control ratios between 2.0 and 9.0 were obtained by comparison of the respiratory rates of cabbage mitochondria in the presence and in the absence of individual components of the system used to provide ADP and by comparing the rates before and after exhaustion of added ADP. These results indicate that resp...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.43.3.327
更新日期:1968-03-01 00:00:00
abstract::Previous evidence has suggested that SPA1 is a signal transduction component that appears to require phytochrome A for function in seedling photomorphogenesis. Using digital image analysis, we examined the time course of growth inhibition induced by red light in spa1 mutants to test the interpretation that SPA1 functi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.126.3.1291
更新日期:2001-07-01 00:00:00
abstract::A pyridoxal 5'-phosphate (PLP)-dependent enzyme, 1-aminocyclopropane-1-carboxylic acid (ACC) synthase (S-adenosyl-L-Met methylthioadenosine-lyase, EC 4.4.1.14), catalyzes the conversion of S-adenosyl-L-methionine (AdoMet) to ACC. A tomato ACC synthase isozyme (LE-ACS2) with a deletion of 46 amino acids at the C termin...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.121.3.913
更新日期:1999-11-01 00:00:00
abstract::A monoclonal antibody raised against carboxyl-linked IAA was used in Western blot analysis of storage proteins from kernels of Avena sativa, Pennisetum americanum, Sorghum bicolor, and Zea mays. IAA or an IAA-like molecule is associated with the ethanolsoluble protein fraction of the seed. Western blotting of commerci...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.96.4.1076
更新日期:1991-08-01 00:00:00
abstract::A ureidoglycolate-degrading activity was analyzed in different organs of chickpea (Cicer arietinum). Activity was detected in all the tissues analyzed, but highest levels of specific activity were found in pods, from which it has been purified and characterized. This is the first ureidoglycolate-degrading activity tha...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.125.2.828
更新日期:2001-02-01 00:00:00
abstract::The effect of strong irradiance (2000 micromole photons per square meter per second) on PSII heterogeneity in intact cells of Chlamydomonas reinhardtii was investigated. Low light (LL, 15 micromole photons per square meter per second) grown C. reinhardtii are photoinhibited upon exposure to strong irradiance, and the ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.92.4.1196
更新日期:1990-04-01 00:00:00
abstract::Fructose 2,6-bisphosphate inhibits phosphoglucomutase noncompetitively with respect to the cofactor glucose 1,6-bisphosphate. Previous studies from our laboratory had shown that phosphoglucomutase was activated by fructose 2,6-bisphosphate in the absence of added glucose 1,6-bisphosphate. The fructose 2,6-bisphosphate...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.88.4.980
更新日期:1988-12-01 00:00:00
abstract::Rice (Oryza sativa) is the staple food for over half the world's population yet may represent a significant dietary source of inorganic arsenic (As), a nonthreshold, class 1 human carcinogen. Rice grain As is dominated by the inorganic species, and the organic species dimethylarsinic acid (DMA). To investigate how As ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.146126
更新日期:2010-01-01 00:00:00
abstract::The sterol composition of C. ellipsoidea was markedly changed when this alga was grown in the presence of 1 mug/g triparanol. Triparanol appears to inhibit the removal of 14alpha-methyl group, the second alkylation at C-24, Delta(7)-reductase, and Delta(8) --> Delta(7)-isomerase. The effect of triparanol in Chlorella ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.52.3.246
更新日期:1973-09-01 00:00:00
abstract::Thermostability of the photosynthetic apparatus of abscisic acid (ABA)-treated seedlings of barley (Hordeum vulgare) was studied by light-scattering and by fluorescence measurements of isolated chloroplasts. ABA treatment markedly decreased heat damage of the chloroplast ultrastructure; an exogenous ABA concentration ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.98.4.1228
更新日期:1992-04-01 00:00:00
abstract::The unicellular green alga Chlorella kessleri can induce monosaccharide-H+ symport catalyzing the energy-dependent transport of D-glucose (D-Glc) and several other pentoses and hexoses across the plasmalemma. The gene coding for the inducible HUP1 monosaccharide-H+ symporter has been cloned and the protein has been ch...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.107.1.33
更新日期:1995-01-01 00:00:00
abstract::When ribulose-1,5-bisphosphate carboxylase is assayed under N(2) using [(3)H]ribulose 1,5-bisphosphate and (14)CO(2), [(3)H]3-phosphoglycerate and [(14)C]3-phosphoglycerate are produced in nonstoichiometric amounts in a ratio which approaches 7 at low concentrations of CO(2) (2 micromolar) assuming a 1:1 ratio at V(ma...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.80.4.1055
更新日期:1986-04-01 00:00:00
abstract::Cesium (Cs) is chemically similar to potassium (K). However, although K is an essential element, Cs is toxic to plants. Two contrasting hypotheses to explain Cs toxicity have been proposed: (1) extracellular Cs+ prevents K+ uptake and, thereby, induces K starvation; and (2) intracellular Cs+ interacts with vital K(+)-...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.046672
更新日期:2004-11-01 00:00:00
abstract::Osmotic adjustment occurred during drought in expanded leaves of sunflowers (Helianthus annuus var Hysun 30) which had been continuously exposed to 660 microliters CO(2) per liter or had been previously acclimated to drought. The effect was greatest when the treatments were combined and was negligible in nonacclimated...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.86.4.1108
更新日期:1988-04-01 00:00:00
abstract::A light-induced shrinkage of chloroplasts in vivo could be detected with chloroplasts isolated within 2 minutes of harvesting pea plants. As determined both by packed volume and Coulter counter, the mean volume of chloroplasts from plants in the dark was 39 mu(3), whereas it was 31 mu(3) for chloroplasts from plants i...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.43.5.781
更新日期:1968-05-01 00:00:00
abstract::Thylakoids isolated from leaves of winter rye (Secale cereale L. cv Puma) grown at either 20 or 5 degrees C were extracted with the nonionic detergents Triton X-100 and octyl glucoside. Less total chlorophyll was extracted from 5 degrees C thylakoids by these detergents under all conditions, including pretreatment wit...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.81.2.471
更新日期:1986-06-01 00:00:00
abstract::Cell-free preparations from seeds of Marah macrocarpus L. and Malus domestica L. catalyzed the conversion of gibberellin A(9) (GA(9)) and 2,3-dehydroGA(9) to GA(7); GA(9) was also metabolized to GA(4) in a branch pathway. The preparation from Marah seeds also metabolized GA(5) to GA(3) in high yield; GA(6) was a minor...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.94.1.132
更新日期:1990-09-01 00:00:00
abstract::Signals can be perceived and amplified at the cell membrane by receptors coupled to the production of a variety of second messengers, including myoinositol 1,4,5-trisphosphate [Ins(1,4,5)P(3)]. The myoinositol polyphosphate 5-phosphatases (5PTases; EC 3.1.3.56) comprise a large protein family that hydrolyzes 5-phospha...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.089474
更新日期:2007-03-01 00:00:00
abstract::The assay of acetyl-CoA carboxylase (EC 6.4.1.2) does not follow ideal zero-order kinetics when assayed in a crude extract from wheat (Triticum aestivum L.) germ. Our results show that the lack of ideality is the consequence of contamination by ATPase and adenylate kinase. These enzyme activities generate significant ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.72.1.50
更新日期:1983-05-01 00:00:00
abstract::Rates of assimilation and transpiration in Eucalyptus pauciflora Sieb. ex Spreng were measured at various ambient partial pressures of CO(2) and various irradiances and were used to estimate leaf conductance and intercellular partial pressure of CO(2). The responses of leaf conductance and rate of assimilation to chan...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.62.4.670
更新日期:1978-10-01 00:00:00