Abstract：
:Cysthathionine [gamma]-synthase (CS), an enzyme involved in methionine biosynthesis, was purified from an acetone powder prepared from wheat (Triticum aestivum L.). After several chromatographic steps and radiolabeling of the partially purified enzyme with sodium cyanoboro[3H]hydride, a single polypeptide with a molecular weight of 34,500 was isolated by sodium dodecyl sulfate-high performance electrophoresis chromatography. Since the molecular weight of the native enzyme was 155,000, CS apparently consists of four identical subunits. The pyridoxal 5[prime]-phosphate-dependent forward reaction has a pH optimum of 7.5 and follows a hybrid ping-pong mechanism with Km values of 3.6 mM and 0.5 mM for L-homoserine phosphate and L-cysteine, respectively. L-Cysteine methyl ester, thioglycolate methyl ester, and sodium sulfide were also utilized as thiol substrates. The latter observation suggests that CS and phosphohomoserine sulfhydrase might be a single enzyme. CS does not seem to be a regulatory enzyme but was irreversibly inhibited by DL-propargylglycine (Ki = 45 [mu]M, Kinact = 0.16 min-1). Furthermore, the homoserine phosphate analogs 4-(phosphonomethyl)-pyridine-2-carboxylic acid, Z-3-(2-phosphonoethen-1-yl)pyridine-2-carboxylic acid, and DL-E-2-amino-5-phosphono-3-pentenoic acid acted as reversible competitive inhibitors with Ki values of 45, 40, and 1.1 [mu]M, respectively.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Kreft BD,Townsend A,Pohlenz HD,Laber Bdoi
10.1104/pp.104.4.1215subject
Has Abstractpub_date
1994-04-01 00:00:00pages
1215-1220issue
4eissn
0032-0889issn
1532-2548pii
104/4/1215journal_volume
104pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract：:Chloroplast division is driven by a macromolecular complex containing components that are positioned on the cytosolic surface of the outer envelope, the stromal surface of the inner envelope, and in the intermembrane space. The only constituents of the division apparatus identified thus far are the tubulin-like protei...
journal_title：Plant physiology
pub_type： 杂志文章
doi：
更新日期：2001-12-01 00:00:00
abstract：:The pyruvate dehydrogenase complex has been purified 76-fold, to a specific activity of 0.6 mumoles per minute per milligram protein, beginning with isolated pea (Pisum sativum L. var Little Marvel) chloroplasts. Purification was accomplished by rate zonal sedimentation, polyethyleneglycol precipitation, and ethyl-aga...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.77.3.571
更新日期：1985-03-01 00:00:00
abstract：:After 3 days of exposure to 10(-3) and 10(-4) M decenylsuccinic acid, winter wheat plants wilted and died. Decenylsuccinate at 10(-3) M inhibited (32)P uptake by barley roots and wheat roots and resulted in significant (P
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.45.1.1
更新日期：1970-01-01 00:00:00
abstract：:The accumulation of delta-aminolevulinic acid and activities of delta-aminolevulinic acid dehydrase were examined in the marine diatom, Skeletonema costatum, grown in the presence of levulinic acid. Levulinic acid concentrations greater than 10 mm affect growth and morphology, and inhibit chlorophyll synthesis. The al...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.62.4.516
更新日期：1978-10-01 00:00:00
abstract：:The light dependence of D1 phosphorylation is unique to higher plants, being constitutive in cyanobacteria and algae. In a photoautotrophic higher plant, Spirodela oligorrhiza, grown in greenhouse conditions under natural diurnal cycles of solar irradiation, the ratio of phosphorylated versus total D1 protein (D1-P in...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.013441
更新日期：2002-12-01 00:00:00
abstract：:Under controlled environment and/or field conditions, vegetative growth (height, internode length, leaf area, shoot dry weight, grain yield) was greater in an F(1) maize hybrid than in either parental inbred. Endogenous gibberellin (GA)-like substances in apical meristem cylinders were also higher in the hybrid than i...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.71.3.639
更新日期：1983-03-01 00:00:00
abstract：:N(6)-(Delta(2)-isopentenyl)Adenine and N(6)-(Delta(2)-isopentenyl)adenosine were hydroxylated, respectively, to 6-(4-hydroxy-3-methyl-trans-2-butenylamino)purine and 6-(4-hydroxy-3-methyl-trans-2-butenylamino) -9-beta-ribofuranosylpurine in the presence of NADPH and the microsomal fraction from cauliflowers (Brassica ...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.75.2.442
更新日期：1984-06-01 00:00:00
abstract：:In plants, bZIP (basic leucine zipper) transcription factors regulate diverse processes such as development and stress responses. However, few of these transcription factors have been functionally characterized in maize (Zea mays). In this study, we characterized the bZIP transcription factor gene ZmbZIP4 from maize. ...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.18.00436
更新日期：2018-10-01 00:00:00
abstract：:After cold treatment of seedlings of winter wheat (Triticum aestivum L.), levels of hydrogen peroxide in the leaves were measured. The concentration of hydrogen peroxide increased to about three times the control level within a few minutes, and returned to the normal level in 15 to 20 minutes. The elevated level of hy...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.97.3.1265
更新日期：1991-11-01 00:00:00
abstract：:Extracts of maize leaf sheath tissue deaminate both l-phenylalanine and l-tyrosine. The activities with both substrates are enhanced by treating the plant with gibberellic acid. Both activities decrease rapidly at the same rate when tissue is incubated in a moist atmosphere, and this decrease can be slowed by treatmen...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.50.4.480
更新日期：1972-10-01 00:00:00
abstract：:A Sclerotinia enzyme preparation with predominant exo-beta-(1-->3)-glucanase activity has the capacity to mediate the formation of tetrasaccharide from 3-O-beta-cellobiosyl-d-glucose or cellotriose, and a pentasaccharide from 3-O-beta-cellotriosyl-d-glucose or cellotetraose. Transglucosylation is not observed when the...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.64.2.193
更新日期：1979-08-01 00:00:00
abstract：:Leaf discs and detached leaves exposed to l-cysteine emitted a volatile sulfur compound which was proven by gas chromatography to be H(2)S. This phenomenon was demonstrated in all nine species tested (Cucumis sativus, Cucurbita pepo, Nicotiana tabacum, Coleus blumei, Beta vulgaris, Phaseolus vulgaris, Medicago sativa,...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.70.2.430
更新日期：1982-08-01 00:00:00
abstract：:The petunia (Petunia [Mitchell]) chloroplast proteins, the chlorophyll a/b-binding (Cab) proteins, and the small subunit of ribulose bisphosphate carboxylase (RbcS) are encoded by nuclear genes that are expressed in a light-dependent manner. The steady-state concentrations of five cab mRNAs vary with a dramatic circad...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.89.3.776
更新日期：1989-03-01 00:00:00
abstract：:Bicarbonate-(14)C was provided to 5- and 11-day-old suspension cultures of Paul's Scarlet rose, and the incorporation of (14)C into lipid, protein, amino acids, and organic acids was determined. The rate of bicarbonate uptake was approximately the same by 5- and 11-day-old cells, but the distribution of (14)C among ce...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.55.4.643
更新日期：1975-04-01 00:00:00
abstract：:The OsCPK4 gene is a member of the complex gene family of calcium-dependent protein kinases in rice (Oryza sativa). Here, we report that OsCPK4 expression is induced by high salinity, drought, and the phytohormone abscisic acid. Moreover, a plasma membrane localization of OsCPK4 was observed by transient expression as...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.113.230268
更新日期：2014-06-01 00:00:00
abstract：:A number of environmental stresses can lead to enhanced production of superoxide within plant tissues, and plants are believed to rely on the enzyme superoxide dismutase (SOD) to detoxify this reactive oxygen species. We have identified seven cDNAs and genes for SOD in Arabidopsis. These consist of three CuZnSODs (CSD...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.118.2.637
更新日期：1998-10-01 00:00:00
abstract：:Water deficit during meiosis in pollen mother cells of wheat (Triticum aestivum L.) induces male sterility, which can reduce grain set by 40 to 50%. In plants stressed during meiosis and then rewatered, division of pollen mother cells proceeds normally but subsequent pollen development is arrested 3 or 4 d later. An i...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.111.1.137
更新日期：1996-05-01 00:00:00
abstract：:Iron (Fe) is an essential mineral nutrient and an important factor for the composition of natural plant communities. Low Fe availability in aerated soils with neutral or alkaline pH has led to the evolution of elaborate mechanisms that extract Fe from the soil solution. In Arabidopsis (Arabidopsis thaliana), Fe is acq...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.18.00178
更新日期：2018-05-01 00:00:00
abstract：:Mitogen-activated protein kinase (MAPK) cascades play important roles in disease resistance in model plant species such as Arabidopsis (Arabidopsis thaliana) and tobacco (Nicotiana tabacum). However, the importance of MAPK signaling pathways in the disease resistance of crops is still largely uninvestigated. To better...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.111.185686
更新日期：2011-11-01 00:00:00
abstract：:Blue light is known to induce suppression of stem elongation. To avoid the complication of blue-light-induced transformation of phytochrome we have adapted the procedure of measuring blue-light-induced suppression of stem elongation in Pisum sativum L. var Alaska grown under continuous red light. The resulting fluence...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.89.2.544
更新日期：1989-02-01 00:00:00
abstract：:Experiments were conducted on Nicotiana tabacum, L. to study the relation in the grana among chlorophylls, carotenoids, and proteins. The effect of iron chlorosis on protein and pigment synthesis was studied at different stages of chlorosis using glycine-U-C(14). Pigments were separated by thin layer chromatography.Ch...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.41.3.415
更新日期：1966-03-01 00:00:00
abstract：:Traditional virus-induced gene silencing (VIGS) is a powerful virus-based short interfering RNA-mediated RNA silencing technique for plant functional genomics. Besides short interfering RNAs, microRNAs (miRNAs) have also been shown to regulate gene expression by RNA silencing in various organisms. However, plant virus...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.110.155796
更新日期：2010-06-01 00:00:00
abstract：:In leaves of two starch-related single-knockout lines lacking either the cytosolic transglucosidase (also designated as disproportionating enzyme 2, DPE2) or the maltose transporter (MEX1), the activity of the plastidial phosphorylase isozyme (PHS1) is increased. In both mutants, metabolism of starch-derived maltose i...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.113.227843
更新日期：2014-02-01 00:00:00
abstract：:Polyadenylated RNA was isolated from maize leaves and translated in vitro. In agreement with a previous report by others, we found among the translation products a 110-kilodalton pyruvate orthophosphate dikinase (PPDK) precursor that is about 16 kilodaltons larger than the polypeptide isolated from cells. This maize P...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.78.4.807
更新日期：1985-08-01 00:00:00
abstract：:Transgenic tomato (Solanum lycopersicum) plants in which either mitochondrial malate dehydrogenase or fumarase was antisense inhibited have previously been characterized to exhibit altered photosynthetic metabolism. Here, we demonstrate that these manipulations also resulted in differences in root growth, with both tr...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.108.130518
更新日期：2009-02-01 00:00:00
abstract：:Root and stem segments from soybean (Merrill cv. ;Bragg') showed an enhanced chemiluminescence upon mechanical injury. Roots emitted more light than did stems. Light emission was diminished by CN(-) and N(3) (-) but was not affected by rotenone and antimycin A. Catalase quenched chemiluminescence in wounded root segme...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.67.1.43
更新日期：1981-01-01 00:00:00
abstract：:The ontogeny of seed structure and the accumulation of seed storage substances is the result of a determinant genetic program. Using RNA interference, the synthesis of soybean (Glycine max) glycinin and conglycinin storage proteins has been suppressed. The storage protein knockdown (SP-) seeds are overtly identical to...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.111.173807
更新日期：2011-05-01 00:00:00
abstract：:Cyclin-dependent kinases (CDKs) form a conserved superfamily of eukaryotic serine-threonine protein kinases whose activity requires the binding of a cyclin protein. CDKs are involved in many aspects of cell biology and notably in the regulation of the cell cycle. Three cDNAs encoding a C-type CDK, and a member of each...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.126.4.1403
更新日期：2001-08-01 00:00:00
abstract：:Endosperm liquid bathes the embryo of Phaseolus vulgaris from the heart stage through the late cotyledon state. This liquid was aspirated from many ovules of the same stage, pooled, and analyzed for the following constituents and parameters: [List: see text]. ...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.51.3.454
更新日期：1973-03-01 00:00:00
abstract：:Biochemical changes related to cyanogenesis (hydrogen cyanide production) were monitored during maturation of black cherry (Prunus serotina Ehrh.) fruits. At weekly intervals from flowering until maturity, fruits (or selected parts thereof) were analyzed for (a) fresh and dry weights, (b) prunasin and amygdalin levels...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.98.4.1423
更新日期：1992-04-01 00:00:00
