Superoxide dismutase in Arabidopsis: an eclectic enzyme family with disparate regulation and protein localization.

Abstract:

:A number of environmental stresses can lead to enhanced production of superoxide within plant tissues, and plants are believed to rely on the enzyme superoxide dismutase (SOD) to detoxify this reactive oxygen species. We have identified seven cDNAs and genes for SOD in Arabidopsis. These consist of three CuZnSODs (CSD1, CSD2, and CSD3), three FeSODs (FSD1, FSD2, and FSD3), and one MnSOD (MSD1). The chromosomal location of these seven SOD genes has been established. To study this enzyme family, antibodies were generated against five proteins: CSD1, CSD2, CSD3, FSD1, and MSD1. Using these antisera and nondenaturing-polyacrylamide gel electrophoresis enzyme assays, we identified protein and activity for two CuZnSODs and for FeSOD and MnSOD in Arabidopsis rosette tissue. Additionally, subcellular fractionation studies revealed the presence of CSD2 and FeSOD protein within Arabidopsis chloroplasts. The seven SOD mRNAs and the four proteins identified were differentially regulated in response to various light regimes, ozone fumigation, and ultraviolet-B irradiation. To our knowledge, this is the first report of a large-scale analysis of the regulation of multiple SOD proteins in a plant species.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Kliebenstein DJ,Monde RA,Last RL

doi

10.1104/pp.118.2.637

subject

Has Abstract

pub_date

1998-10-01 00:00:00

pages

637-50

issue

2

eissn

0032-0889

issn

1532-2548

journal_volume

118

pub_type

杂志文章
  • Replication of cauliflower mosaic virus DNA in leaves and suspension culture protoplasts of cotton.

    abstract::Cauliflower mosaic virus (CaMV) replicated in protoplasts and in inoculated leaves of the non-host, cotton (Gossypium hirsutum, L.). Protoplasts prepared from suspension-cultured cotton cells were infected by incubation with liposome-encapsulated CaMV virions. During a 1-week culture period the amount of CaMV nucleic ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.83.3.633

    authors: Hussain MM,Melcher U,Whittle T,Williams A,Brannan CM,Mitchell ED

    更新日期:1987-03-01 00:00:00

  • Extracellular Matrix Assembly in Diatoms (Bacillariophyceae) (I. A Model of Adhesives Based on Chemical Characterization and Localization of Polysaccharides from the Marine Diatom Achnanthes longipes and Other Diatoms).

    abstract::Extracellular adhesives from the diatoms Achnanthes longipes, Amphora coffeaeformis, Cymbella cistula, and Cymbella mexicana were characterized by monosaccharide and methylation analysis, lectin-fluorescein isothiocyanate localization, and cytochemical staining. Polysaccharide was the major component of adhesives form...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.4.1059

    authors: Wustman BA,Gretz MR,Hoagland KD

    更新日期:1997-04-01 00:00:00

  • Cold hardening in citrus stems.

    abstract::Stem cold hardening developed to different levels in citrus types tested in controlled environments. Exotherms indicated ice spread was more uniform and rapid in unhardened than in cold-hardened stems. All attempts to inhibit the functioning of citrus leaves resulted in less cold hardening in the stems. Citrus leaves ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.56.4.540

    authors: Yelenosky G

    更新日期:1975-10-01 00:00:00

  • Developmental Changes in the Free Amino Acid Pool and Total Protein Amino Acids of Pea Cotyledons (Pisum sativum L.).

    abstract::Changes in the levels of twenty-two free amino acids and in the amino acid composition of the total protein were measured throughout the development of cotyledons of a dwarf garden pea, Pisum sativum cv Greenfeast, grown in a constant environment. A sensitive double-isotope dansylation technique was used. Fresh weight...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.72.2.492

    authors: Macnicol PK

    更新日期:1983-06-01 00:00:00

  • Identification and characterization of stretch-activated ion channels in pollen protoplasts.

    abstract::Pollen tube growth requires a Ca2+ gradient, with elevated levels of cytosolic Ca2+ at the growing tip. This gradient's magnitude oscillates with growth oscillation but is always maintained. Ca2+ influx into the growing tip is necessary, and its magnitude also oscillates with growth. It has been widely assumed that st...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.041483

    authors: Dutta R,Robinson KR

    更新日期:2004-07-01 00:00:00

  • Induction and functional analysis of two reduced nicotinamide adenine dinucleotide phosphate-dependent glutathione peroxidase-like proteins in Synechocystis PCC 6803 during the progression of oxidative stress.

    abstract::Synechocystis PCC 6803 contains two types of glutathione peroxidase-like proteins (GPX-1 and GPX-2) that utilize NADPH but not reduced glutathione and unsaturated fatty acid hydroperoxides or alkyl hydroperoxides. The steady-state transcript level of gpx-1 gradually increased under oxidative stress conditions imposed ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.044842

    authors: Gaber A,Yoshimura K,Tamoi M,Takeda T,Nakano Y,Shigeoka S

    更新日期:2004-09-01 00:00:00

  • Long distance translocation of sucrose, serine, leucine, lysine, and carbon dioxide assimilates: I. Soybean.

    abstract::To determine the selectivity of movement of amino acids from source leaves to sink tissues in soybeans (Glycine max [L.] Merr. ;Wells'), (14)C-labeled serine, leucine, or lysine was applied to an abraded spot on a fully expanded trifoliolate leaflet, and an immature sink leaf three nodes above was monitored with a GM ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.59.2.217

    authors: Housley TL,Peterson DM,Schrader LE

    更新日期:1977-02-01 00:00:00

  • ECHIDNA protein impacts on male fertility in Arabidopsis by mediating trans-Golgi network secretory trafficking during anther and pollen development.

    abstract::The trans-Golgi network (TGN) plays a central role in cellular secretion and has been implicated in sorting cargo destined for the plasma membrane. Previously, the Arabidopsis (Arabidopsis thaliana) echidna (ech) mutant was shown to exhibit a dwarf phenotype due to impaired cell expansion. However, ech also has a prev...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.227769

    authors: Fan X,Yang C,Klisch D,Ferguson A,Bhaellero RP,Niu X,Wilson ZA

    更新日期:2014-03-01 00:00:00

  • Transcriptome Association Identifies Regulators of Wheat Spike Architecture.

    abstract::The architecture of wheat (Triticum aestivum) inflorescence and its complexity is among the most important agronomic traits that influence yield. For example, wheat spikes vary considerably in the number of spikelets, which are specialized reproductive branches, and the number of florets, which are spikelet branches t...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.17.00694

    authors: Wang Y,Yu H,Tian C,Sajjad M,Gao C,Tong Y,Wang X,Jiao Y

    更新日期:2017-10-01 00:00:00

  • Characterization of the Proton-Translocating Cytochrome c Oxidase Activity in the Plasma Membrane of Intact Anacystis nidulans Spheroplasts.

    abstract::Intact spheroplasts of the cyanobacterium (blue-green alga) Anacystis nidulans oxidized various exogenous c-type cytochromes with concomitant outward proton translocation while exogenous ferricytochrome c was not reduced. The H(+)/e(-) stoichiometry was close to 1 with each of the cytochromes and did not depend on the...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.75.4.968

    authors: Peschek GA

    更新日期:1984-08-01 00:00:00

  • Characterization of the entire cystatin gene family in barley and their target cathepsin L-like cysteine-proteases, partners in the hordein mobilization during seed germination.

    abstract::Plant cystatins are inhibitors of cysteine-proteases of the papain C1A and legumain C13 families. Cystatin data from multiple plant species have suggested that these inhibitors act as defense proteins against pests and pathogens and as regulators of protein turnover. In this study, we characterize the entire cystatin ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.146019

    authors: Martinez M,Cambra I,Carrillo L,Diaz-Mendoza M,Diaz I

    更新日期:2009-11-01 00:00:00

  • Phosphorylation of MAP65-1 by Arabidopsis Aurora Kinases Is Required for Efficient Cell Cycle Progression.

    abstract::Aurora kinases are key effectors of mitosis. Plant Auroras are functionally divided into two clades. The alpha Auroras (Aurora1 and Aurora2) associate with the spindle and the cell plate and are implicated in controlling formative divisions throughout plant development. The beta Aurora (Aurora3) localizes to centromer...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.01602

    authors: Boruc J,Weimer AK,Stoppin-Mellet V,Mylle E,Kosetsu K,Cedeño C,Jaquinod M,Njo M,De Milde L,Tompa P,Gonzalez N,Inzé D,Beeckman T,Vantard M,Van Damme D

    更新日期:2017-01-01 00:00:00

  • Characteristics of Nitrate Reductase-inactivating Proteins Obtained from Corn Roots and Rice Cell Cultures.

    abstract::Nitrate reductase (NR)-inactivating proteins from corn roots (Wf-9 x 38-11) and rice cell suspension cultures were tested against a partially purified NR obtained from corn leaves (W64A x W182E). The corn protein was purified 921-fold and the rice protein, 1,660-fold using standard purification procedures. Approximate...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.65.1.141

    authors: Yamaya T,Oaks A,Boesel IL

    更新日期:1980-01-01 00:00:00

  • Fractionation of Carbon Isotopes during Biogenesis of Atmospheric Isoprene.

    abstract::The stable carbon isotope composition of isoprene emitted from leaves of red oak (Quercus rubra L.) was measured. Isoprene was depleted in (13)C relative to carbon recently fixed by photosynthesis. The difference in isotope composition between recently fixed carbon and emitted isoprene was independent of the isotopic ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.97.1.463

    authors: Sharkey TD,Loreto F,Delwiche CF,Treichel IW

    更新日期:1991-09-01 00:00:00

  • Isolation of a kaurene synthetase inhibitor from castor bean seedlings and cell suspension cultures.

    abstract::Biosynthesis of ent-kaurene was investigated in extracts of cell suspension cultures and seedlings of castor bean. Both cell-free extracts contain an inhibitor of kaurene synthetase. The inhibition affects mainly the cyclization of geranylgeranyl pyrophosphate to copalyl pyrophosphate (activity A) and has little or no...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.67.6.1169

    authors: Gafni Y,Shechter I

    更新日期:1981-06-01 00:00:00

  • Role of roots in regulating the growth rate and cytokinin content in leaves.

    abstract::The role of roots in the enhancement of cytokinin content and leaf growth of Phaseolus vulgaris plants after decapitation and partial defoliation was investigated. Partial excision of the roots of plants which were decapitated above the primary leaf node resulted in a reduction of leaf growth and soluble proteins accu...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.73.1.76

    authors: Carmi A,Staden JV

    更新日期:1983-09-01 00:00:00

  • Photosynthesis, leaf resistances, and ribulose-1,5-bisphosphate carboxylase degradation in senescing barley leaves.

    abstract::The relationship between loss of ribulose-1,5-bisphosphate carboxylase (RuBPCase) and the decline in photosynthesis during the senescence of barley primary leaves was assessed. Loss of RuBPCase accounted for about 85% of the decrease in soluble protein. RuBPCase was highly correlated with in vitro RuBPCase activity (r...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.65.6.1103

    authors: Friedrich JW,Huffaker RC

    更新日期:1980-06-01 00:00:00

  • Chlorophyll synthesis in dark-grown pine primary needles

    abstract::The pigment content of dark-grown primary needles of Pinus jeffreyi L. and Pinus sylvestris L. was determined by high-performance liquid chromatography. The state of protochlorophyllide a and of chlorophylls during dark growth were analyzed by in situ 77 K fluorescence spectroscopy. Both measurements unambiguously dem...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.118.4.1159

    authors: Schoefs B,Franck F

    更新日期:1998-12-01 00:00:00

  • Sucrose phosphate synthase and sucrose accumulation at low temperature.

    abstract::The influence of growth temperature on the free sugar and sucrose phosphate synthase content and activity of spinach (Spinacia oleracea) leaf tissue was studied. When plants were grown at 25 degrees C for 3 weeks and then transferred to a constant 5 degrees C, sucrose, glucose, and fructose accumulated to high levels ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.100.1.502

    authors: Guy CL,Huber JL,Huber SC

    更新日期:1992-09-01 00:00:00

  • Arabidopsis Novel Glycine-Rich Plasma Membrane PSS1 Protein Enhances Disease Resistance in Transgenic Soybean Plants.

    abstract::Nonhost resistance is defined as the immunity of a plant species to all nonadapted pathogen species. Arabidopsis (Arabidopsis thaliana) ecotype Columbia-0 is nonhost to the oomycete plant pathogen Phytophthora sojae and the fungal plant pathogen Fusarium virguliforme that are pathogenic to soybean (Glycine max). Previ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.01982

    authors: Wang B,Sumit R,Sahu BB,Ngaki MN,Srivastava SK,Yang Y,Swaminathan S,Bhattacharyya MK

    更新日期:2018-01-01 00:00:00

  • Rapid Accumulation of Anionic Peroxidases and Phenolic Polymers in Soybean Cotyledon Tissues following Treatment with Phytophthora megasperma f. sp. Glycinea Wall Glucan.

    abstract::Phytophthora megasperma Drechs. f. sp. glycinea Kuan & Erwin (PMG) cell wall glucan has been extensively characterized as an elicitor of the pterocarpan phytoalexins, the glyceollins in soybean (Glycine max L.). Just recently, this glucan was shown to be a potent elicitor of conjugates of the isoflavones, daidzein and...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.97.4.1445

    authors: Graham MY,Graham TL

    更新日期:1991-12-01 00:00:00

  • Polygalacturonase-inhibiting protein interacts with pectin through a binding site formed by four clustered residues of arginine and lysine.

    abstract::Polygalacturonase-inhibiting protein (PGIP) is a cell wall protein that inhibits fungal polygalacturonases (PGs) and retards the invasion of plant tissues by phytopathogenic fungi. Here, we report the interaction of two PGIP isoforms from Phaseolus vulgaris (PvPGIP1 and PvPGIP2) with both polygalacturonic acid and cel...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.076950

    authors: Spadoni S,Zabotina O,Di Matteo A,Mikkelsen JD,Cervone F,De Lorenzo G,Mattei B,Bellincampi D

    更新日期:2006-06-01 00:00:00

  • Saponin biosynthesis in Saponaria vaccaria. cDNAs encoding beta-amyrin synthase and a triterpene carboxylic acid glucosyltransferase.

    abstract::Saponaria vaccaria (Caryophyllaceae), a soapwort, known in western Canada as cowcockle, contains bioactive oleanane-type saponins similar to those found in soapbark tree (Quillaja saponaria; Rosaceae). To improve our understanding of the biosynthesis of these saponins, a combined polymerase chain reaction and expresse...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.088484

    authors: Meesapyodsuk D,Balsevich J,Reed DW,Covello PS

    更新日期:2007-02-01 00:00:00

  • Phytochrome action on chlorophyll synthesis-a study of the escape from photoreversibility.

    abstract::A brief red light pretreatment (pulse), operating through phytochrome, stimulates the synthesis of chlorophyll a and b in Sorghum vulgare shoots that are placed in continuous saturating white light. The red light effect is fully reversible by a far-red (756 nanometers) light pulse for 45 minutes. Thereafter, escape fr...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.70.3.863

    authors: Oelze-Karow H,Mohr H

    更新日期:1982-09-01 00:00:00

  • Photocontrol of Anthocyanin Synthesis: V. Further Evidence against the Involvement of Photosynthesis in High Irradiance Reaction Anthocyanin Synthesis of Young Seedlings.

    abstract::Streptomycin and chloramphenicol inhibit the development of the photosynthetic apparatus and enhance anthocyanin synthesis in tomato (Lycopersican esculentum, cv. Beefsteak) and red cabbage (Brassica oleracea, cv. Red Acre) seedlings. The two antibiotics do not affect the basic features of light-dependent anthocyanin ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.58.2.214

    authors: Mancinelli AL,Yang CP,Rabino I,Kuzmanoff KM

    更新日期:1976-08-01 00:00:00

  • Phosphatidylinositol specific phospholipase C of plant stems : membrane associated activity concentrated in plasma membranes.

    abstract::A phosphatidylinositol-specific phospholipase C of plant stems (EC 3.1.4.10) assayed at pH 6.6 and at 30 degrees C cleaved phosphatidylinositol such that more than 85% of the product was inositol-1-phosphate. Other phospholipids were cleaved 5 to 10% or less under these conditions. The phospholipase had both a soluble...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.85.4.1151

    authors: Pfaffmann H,Hartmann E,Brightman AO,Morré DJ

    更新日期:1987-12-01 00:00:00

  • Developmental and environmental regulation of the Nicotiana plumbaginifolia cytosolic Cu/Zn-superoxide dismutase promoter in transgenic tobacco.

    abstract::Superoxide dismutases (SODs) play a key role in the cellular defense against reactive oxygen species. To study the transcriptional regulation at the cellular level, the promoter of the Nicotiana plumbaginifolia cytosolic gene encoding Cu/ZnSOD (SODCc) was fused to the beta-glucuronidase (GUS) reporter gene (gusA) and ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.3.873

    authors: Hérouart D,Van Montagu M,Inzé D

    更新日期:1994-03-01 00:00:00

  • Characterization of Hypoxically Inducible Lactate Dehydrogenase in Maize.

    abstract::Oxygen deprivation induces a wide variety of genes, but the most extensively studied are those encoding enzymes of the glycolytic pathway. Lactate dehydrogenase (LDH, EC 1.1.1.27) activity increases up to 3.5-fold in maize (Zea mays L.) roots during several days of hypoxic induction. This increase in activity is accom...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.3.1015

    authors: Christopher ME,Good AG

    更新日期:1996-11-01 00:00:00

  • Association of Plant p40 Protein with Ribosomes Is Enhanced When Polyribosomes Form during Periods of Active Tissue Growth.

    abstract::p40s are acidic proteins of eukaryotic cells occurring either free in the cytoplasm or in association with ribosomes, the latter occurring in both monosomes and polysomes. p40s may play a role in the regulation of protein synthesis, although the exact mechanism is not known. Leaves of all 10 plant species examined her...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.111.2.559

    authors: Garcia-Hernandez M,Davies E,Baskin TI,Staswick PE

    更新日期:1996-06-01 00:00:00

  • Slow Inactivation of Ribulosebisphosphate Carboxylase during Catalysis Is Caused by Accumulation of a Slow, Tight-Binding Inhibitor at the Catalytic Site.

    abstract::The slow inactivation which accompanies catalysis by higher-plant ribulose-P(2) carboxylase-oxygenase (Rubisco) in vitro was only partially reversed when the enzyme was gel filtered to remove small molecules. However, gel filtration or dialysis in the presence of high SO(2-) (4) concentrations induced full recovery. T...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.93.4.1390

    authors: Edmondson DL,Badger MR,Andrews TJ

    更新日期:1990-08-01 00:00:00