A common fungal associate of the spruce bark beetle metabolizes the stilbene defenses of Norway spruce.

Abstract:

:Norway spruce (Picea abies) forests suffer periodic fatal attacks by the bark beetle Ips typographus and its fungal associate, Ceratocystis polonica. Norway spruce protects itself against fungal and bark beetle invasion by the production of terpenoid resins, but it is unclear whether resins or other defenses are effective against the fungus. We investigated stilbenes, a group of phenolic compounds found in Norway spruce bark with a diaryl-ethene skeleton with known antifungal properties. During C. polonica infection, stilbene biosynthesis was up-regulated, as evidenced by elevated transcript levels of stilbene synthase genes. However, stilbene concentrations actually declined during infection, and this was due to fungal metabolism. C. polonica converted stilbenes to ring-opened, deglycosylated, and dimeric products. Chromatographic separation of C. polonica protein extracts confirmed that these metabolites arose from specific fungal enzyme activities. Comparison of C. polonica strains showed that rapid conversion of host phenolics is associated with higher virulence. C. polonica is so well adapted to its host's chemical defenses that it is even able to use host phenolic compounds as its sole carbon source.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Hammerbacher A,Schmidt A,Wadke N,Wright LP,Schneider B,Bohlmann J,Brand WA,Fenning TM,Gershenzon J,Paetz C

doi

10.1104/pp.113.218610

subject

Has Abstract

pub_date

2013-07-01 00:00:00

pages

1324-36

issue

3

eissn

0032-0889

issn

1532-2548

pii

pp.113.218610

journal_volume

162

pub_type

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