Abstract:
:Electron paramagnetic resonance has been used to quantitate reduction of ferric ion bound to hydroxamate siderophores by iron-stressed fungi (DJ Ecker et al. 1982 J Biol Chem 257:8623-8626) and by iron-stressed Chlorella vulgaris (ATCC 11468) (FCT Allnutt, WD Bonner 1984 J Plant Nutr 7: 427-435). Application of this technique to quantitation of bound ferric ion has been questioned due to the possibility of freezing artifacts. Dipole-dipole interaction which resulted in broadening of the g = 4.3 signal was observed. Quantitation was affected when peak to trough amplitude was applied to quantitate the ferric ion but could be corrected by the application of internal standards and maintenance of constant cell content in the samples.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Allnutt FC,Bonner WDdoi
10.1104/pp.83.4.739subject
Has Abstractpub_date
1987-04-01 00:00:00pages
739-41issue
4eissn
0032-0889issn
1532-2548journal_volume
83pub_type
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