Ice-cap. A high-throughput method for capturing plant tissue samples for genotype analysis.


:High-throughput genotype screening is rapidly becoming a standard research tool in the post-genomic era. A major bottleneck currently exists, however, that limits the utility of this approach in the plant sciences. The rate-limiting step in current high-throughput pipelines is that tissue samples from living plants must be collected manually, one plant at a time. In this article I describe a novel method for harvesting tissue samples from living seedlings that eliminates this bottleneck. The method has been named Ice-Cap to reflect the fact that ice is used to capture the tissue samples. The planting of seeds, growth of seedlings, and harvesting of tissue are all performed in a 96-well format. I demonstrate the utility of this system by using tissue harvested by Ice-Cap to genotype a population of Arabidopsis seedlings that is segregating a previously characterized mutation. Because the harvesting of tissue is performed in a nondestructive manner, plants with the desired genotype can be transferred to soil and grown to maturity. I also show that Ice-Cap can be used to analyze genomic DNA from rice (Oryza sativa) seedlings. It is expected that this method will be applicable to high-throughput screening with many different plant species, making it a useful technology for performing marker assisted selection.


Plant Physiol


Plant physiology


Krysan P




Has Abstract


2004-07-01 00:00:00














  • Identification and characterization of nucleotide-binding site-leucine-rich repeat genes in the model plant Medicago truncatula.

    abstract::The nucleotide-binding site (NBS)-Leucine-rich repeat (LRR) gene family accounts for the largest number of known disease resistance genes, and is one of the largest gene families in plant genomes. We have identified 333 nonredundant NBS-LRRs in the current Medicago truncatula draft genome (Mt1.0), likely representing ...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Ameline-Torregrosa C,Wang BB,O'Bleness MS,Deshpande S,Zhu H,Roe B,Young ND,Cannon SB

    更新日期:2008-01-01 00:00:00

  • The alternative oxidase of plant mitochondria is involved in the acclimation of shoot growth at low temperature. A study of Arabidopsis AOX1a transgenic plants.

    abstract::The alternative oxidase (AOX) pathway of plant mitochondria uncouples respiration from mitochondrial ATP production and may ameliorate plant performance under stressful environmental conditions, such as cold temperatures, by preventing excess accumulation of reactive oxygen species. We tested this model in whole tissu...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Fiorani F,Umbach AL,Siedow JN

    更新日期:2005-12-01 00:00:00

  • The Arabidopsis embryo mutant schlepperless has a defect in the chaperonin-60alpha gene.

    abstract::We identified a T-DNA-generated mutation in the chaperonin-60alpha gene of Arabidopsis that produces a defect in embryo development. The mutation, termed schlepperless (slp), causes retardation of embryo development before the heart stage, even though embryo morphology remains normal. Beyond the heart stage, the slp m...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Apuya NR,Yadegari R,Fischer RL,Harada JJ,Zimmerman JL,Goldberg RB

    更新日期:2001-06-01 00:00:00

  • Reduced expression of succinyl-coenzyme A ligase can be compensated for by up-regulation of the gamma-aminobutyrate shunt in illuminated tomato leaves.

    abstract::Increasing experimental evidence suggests that the tricarboxylic acid cycle in plants is of greater importance in illuminated photosynthetic tissues than previously thought. In this study, transgenic tomato (Solanum lycopersicum) plants expressing a fragment of the beta-subunit of succinyl-coenzyme A ligase in either ...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Studart-Guimarães C,Fait A,Nunes-Nesi A,Carrari F,Usadel B,Fernie AR

    更新日期:2007-11-01 00:00:00

  • Differential Activation of Expression of a Suberization-Associated Anionic Peroxidase Gene in Near-Isogenic Resistant and Susceptible Tomato Lines by Elicitors of Verticillium albo-atratrum.

    abstract::We tested whether the expression of the suberization-associated anionic peroxidase gene is involved in the timely appearance of the vascular suberized coating involved in the resistance of a tomato line to Verticillium albo-atrum. The mRNA for this peroxidase appeared at a higher level one day earlier in wound-healing...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Mohan R,Kolattukudy PE

    更新日期:1990-01-01 00:00:00

  • A comprehensive analysis of microProteins reveals their potentially widespread mechanism of transcriptional regulation.

    abstract::Truncated transcription factor-like proteins called microProteins (miPs) can modulate transcription factor activities, thereby increasing transcriptional regulatory complexity. To understand their prevalence, evolution, and function, we predicted over 400 genes that encode putative miPs from Arabidopsis (Arabidopsis t...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Magnani E,de Klein N,Nam HI,Kim JG,Pham K,Fiume E,Mudgett MB,Rhee SY

    更新日期:2014-05-01 00:00:00

  • Characterization of maize elongation factor 1A and its relationship to protein quality in the endosperm.

    abstract::The protein synthesis elongation factor 1A (eEF1A) is a multifunctional protein in eukaryotic cells. In maize (Zea mays L.) endosperm eEF1A co-localizes with actin around protein bodies, and its accumulation is highly correlated with the protein-bound lysine (Lys) content. We purified eEF1A from maize kernels by ammon...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Sun Y,Carneiro N,Clore AM,Moro GL,Habben JE,Larkins BA

    更新日期:1997-11-01 00:00:00

  • Nickel in Plants: II. Distribution and Chemical Form in Soybean Plants.

    abstract::The gross tissue distribution, intracellular fate, and chemical behavior of Ni(2+) in soybean plants (Glycine max cv. Williams) were investigated. Following root absorption, Ni was highly mobile in the plant, with leaves being the major sink in the shoots for Ni during vegetative growth. A senescence >70% of the Ni pr...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Cataldo DA,Garland TR,Wildung RE

    更新日期:1978-10-01 00:00:00

  • Plastidial alpha-glucan phosphorylase is not required for starch degradation in Arabidopsis leaves but has a role in the tolerance of abiotic stress.

    abstract::To study the role of the plastidial alpha-glucan phosphorylase in starch metabolism in the leaves of Arabidopsis, two independent mutant lines containing T-DNA insertions within the phosphorylase gene were identified. Both insertions eliminate the activity of the plastidial alpha-glucan phosphorylase. Measurement of o...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Zeeman SC,Thorneycroft D,Schupp N,Chapple A,Weck M,Dunstan H,Haldimann P,Bechtold N,Smith AM,Smith SM

    更新日期:2004-06-01 00:00:00

  • Specificity of RCN1-mediated protein phosphatase 2A regulation in meristem organization and stress response in roots.

    abstract::Protein dephosphorylation by the serine/threonine protein phosphatase 2A (PP2A) modulates a broad array of cellular functions. PP2A normally acts as a heterotrimeric holoenzyme complex comprising a catalytic subunit bound by regulatory A and B subunits. Characterization of the regulatory A subunit isoforms (ROOTS CURL...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Blakeslee JJ,Zhou HW,Heath JT,Skottke KR,Barrios JA,Liu SY,DeLong A

    更新日期:2008-02-01 00:00:00

  • Radial movement of oxygen in plant roots.

    abstract::The radial movement of oxygen in excised corn and jack bean roots was measured with a platinum wire electrode embedded in the root tissue. Measurements were made with the roots exposed to air and with the roots immersed in nutrient solution in the presence and absence of millimolar sodium azide. Effective rates of oxy...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Fiscus EL,Kramer PJ

    更新日期:1970-06-01 00:00:00

  • Reduction of Iron by Leaf Extracts and Its Significance for the Assay of Fe(II) Iron in Plants.

    abstract::"Active Fe" in plants has generally been interpreted to be ferrous iron. This study evaluated some of the commonly followed active Fe determination procedures based on Fe(II) measurements. In each of 12 species examined, leaf extracts exhibited strong reducing activity and quickly reduced any added Fe(III) with or wit...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Mehrotra SC,Gupta P

    更新日期:1990-07-01 00:00:00

  • A comprehensive analysis of the NADP-malic enzyme gene family of Arabidopsis.

    abstract::The Arabidopsis (Arabidopsis thaliana) genome contains four genes encoding putative NADP-malic enzymes (MEs; AtNADP-ME1-ME4). NADP-ME4 is localized to plastids, whereas the other three isoforms do not possess any predicted organellar targeting sequence and are therefore expected to be cytosolic. The plant NADP-MEs can...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Wheeler MC,Tronconi MA,Drincovich MF,Andreo CS,Flügge UI,Maurino VG

    更新日期:2005-09-01 00:00:00

  • Calcium Levels Affect the Ability to Immunolocalize Calmodulin to Cortical Microtubules.

    abstract::Calcium affects the stability of cortical microtubules (MTs) in lysed protoplasts. This calmodulin (CaM)-mediated interaction may provide a mechanism that serves to integrate cellular behavior with MT function. To test the hypothesis that CaM associates with these MTs, monoclonal antibodies were produced against CaM, ...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Fisher DD,Cyr RJ

    更新日期:1993-10-01 00:00:00

  • A Role for Plant KASH Proteins in Regulating Stomatal Dynamics.

    abstract::Stomatal movement, which regulates gas exchange in plants, is controlled by a variety of environmental factors, including biotic and abiotic stresses. The stress hormone abscisic acid (ABA) initiates a signaling cascade, which leads to increased H2O2 and Ca2+ levels and F-actin reorganization, but the mechanism of, an...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Biel A,Moser M,Meier I

    更新日期:2020-02-01 00:00:00

  • Regulation of phycobilisome structure and gene expression by light intensity.

    abstract::The cyanobacterium Agmenellum quadruplicatum PR-6 (Synechococcus sp PCC 7002) was grown turbidostatically in white light at three levels of irradiance: 20, 200, and 1260 microeinsteins per square meter per second. Phycobilisomes were isolated from each culture and analyzed by absorbance, gel electrophoresis, and elect...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: de Lorimier RM,Smith RL,Stevens SE

    更新日期:1992-03-01 00:00:00

  • Identification of 3-(O-beta-Glucosyl)-2-Indolone-3-Acetylaspartic Acid as a New Indole-3-Acetic Acid Metabolite in Vicia Seedlings.

    abstract::A new indole-3-acetic acid metabolite was isolated from broad bean (Vicia faba L. cv Chukyo) seedlings. It was a conjugate of dioxindole-3-acetic acid, aspartic acid, and glucose and was identified as 3-(O-beta-glucosyl)-2-indolone-3-acetylaspartic acid (molecular weight 484) from ultraviolet, infrared, nuclear magnet...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Tsurumi S,Wada S

    更新日期:1985-11-01 00:00:00

  • Sugar-Binding Activity of Pea Lectin Expressed in White Clover Hairy Roots.

    abstract::Introduction of the pea (Pisum sativum L.) lectin (PSL) gene into white clover (Trifolium repens L.) hairy roots facilitates nodulation by the nitrogen-fixing bacterium Rhizobium leguminosarum biovar viciae, which normally nodulates pea and not white clover (C.L. Diaz, L.S. Melchers, P.J.J. Hooykaas, B.J.J. Lugtenberg...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Diaz CL,Logman T,Stam HC,Kijne JW

    更新日期:1995-12-01 00:00:00

  • Production of volatile amines and skatole at anthesis in some arum lily species.

    abstract::The odoriferous compounds produced at the time of flowering by the inflorescences of 5 arum lily species were condensed in dry ice traps. The components of the condensates were separated by paper chromatography and identified on the basis of position and color reactions in comparison with known compounds. The concentr...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Smith BN,Meeuse BJ

    更新日期:1966-02-01 00:00:00

  • Identification of AtNDI1, an internal non-phosphorylating NAD(P)H dehydrogenase in Arabidopsis mitochondria.

    abstract::Plant mitochondria contain non-phosphorylating NAD(P)H dehydrogenases (DHs) that are not found in animal mitochondria. The physiological function, substrate specificity, and location of enzymes within this family have yet to be conclusively determined. We have linked genome sequence information to protein and biochemi...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Moore CS,Cook-Johnson RJ,Rudhe C,Whelan J,Day DA,Wiskich JT,Soole KL

    更新日期:2003-12-01 00:00:00

  • Targeting tryptophan decarboxylase to selected subcellular compartments of tobacco plants affects enzyme stability and in vivo function and leads to a lesion-mimic phenotype.

    abstract::Tryptophan decarboxylase (TDC) is a cytosolic enzyme that catalyzes an early step of the terpenoid indole alkaloid biosynthetic pathway by decarboxylation of L-tryptophan to produce the protoalkaloid tryptamine. In the present study, recombinant TDC was targeted to the chloroplast, cytosol, and endoplasmic reticulum (...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Di Fiore S,Li Q,Leech MJ,Schuster F,Emans N,Fischer R,Schillberg S

    更新日期:2002-07-01 00:00:00

  • Identification of a cis-regulatory element involved in phytochrome down-regulated expression of the pea small GTPase gene pra2.

    abstract::The pra2 gene encodes a pea (Pisum sativum) small GTPase belonging to the YPT/rab family, and its expression is down-regulated by light, mediated by phytochrome. We have isolated and characterized a genomic clone of this gene and constructed a fusion DNA of its 5'-upstream region in front of the gene for firefly lucif...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Inaba T,Nagano Y,Sakakibara T,Sasaki Y

    更新日期:1999-06-01 00:00:00

  • Photosynthesis: action spectra for leaves in normal and low oxygen.

    abstract::The action spectrum of apparent photosynthesis for attached radish (Raphanus sativus L. var. Early Scarlet Globe) and corn (Zea mays L. var. Pride V.) leaves was measured at 300 mul/l CO(2) and both 21% and 2% O(2). The spectra were measured at light intensities where apparent photosynthesis was proportional to intens...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Bulley NR,Nelson CD,Tregunna EB

    更新日期:1969-05-01 00:00:00

  • Phosphorus Distribution in Red Pine Roots and the Ectomycorrhizal Fungus Hebeloma arenosa.

    abstract::Red pines (Pinus resinosa Ait.) were grown in a pasteurized sandy loam either unamended with phosphate or fertilized with one of two levels of phosphate (34 or 136 mg/kg) as superphosphate, and with and without addition of Hebeloma arenosa inoculum. Shoot and total dry weights of mycorrhizal seedlings grown in soil un...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Macfall JS,Slack SA,Wehrli S

    更新日期:1992-10-01 00:00:00

  • Determination of Abscisic Acid in Pinus densiflora by Selected Ion Monitoring.

    abstract::Abscisic acid (ABA) in the stem of akamatsu (Pinus densiflora) was identified and quantified by gas chromatography-mass spectrometry-selected ion monitoring using hexadeuterated ABA as an internal standard. tert-Butyldimethylsilyl ester was used as a derivative of ABA. This derivative had high sensitivity and selectiv...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Funada R,Sugiyama T,Kubo T,Fushitani M

    更新日期:1988-11-01 00:00:00

  • Responses of transpiration and hydraulic conductance to root temperature in nitrogen- and phosphorus-deficient cotton seedlings.

    abstract::Suboptimal N or P availability and cool temperatures all decrease apparent hydraulic conductance (L) of cotton (Gossypium hirsutum L.) roots. The interaction between nutrient status and root temperature was tested in seedlings grown in nutrient solutions. The depression of L (calculated as the ratio of transpiration r...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Radin JW

    更新日期:1990-03-01 00:00:00


    abstract::The relationship between net photosynthesis and CO(2) concentration was investigated for four species of lichen using an infrared gas analyzer operating in a closed loop system. All species showed a linear relationship at low CO(2) levels (100 microliters per liter) with CO(2) saturation levels being in excess of 400 ...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Green TG,Snelgar WP

    更新日期:1981-07-01 00:00:00

  • Mycorrhiza-Triggered Transcriptomic and Metabolomic Networks Impinge on Herbivore Fitness.

    abstract::Symbioses between plants and mycorrhizal fungi are ubiquitous in ecosystems and strengthen the plants' defense against aboveground herbivores. Here, we studied the underlying regulatory networks and biochemical mechanisms in leaves induced by ectomycorrhizae that modify herbivore interactions. Feeding damage and ovipo...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Kaling M,Schmidt A,Moritz F,Rosenkranz M,Witting M,Kasper K,Janz D,Schmitt-Kopplin P,Schnitzler JP,Polle A

    更新日期:2018-04-01 00:00:00

  • Levels of phosphate esters in spirodela.

    abstract::The duckweed Spirodela oligorrhiza was grown in sterile nutrient solutions that contained 1 mm phosphate-(32)P at various specific activities. In solutions with activities higher than 2 muc per mumole per ml, plant growth was inhibited after a time, and the physical appearance of the plants was affected. The critical ...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Bieleski RL

    更新日期:1968-08-01 00:00:00

  • Characterization of root hair cell walls as potential barriers to the infection of plants by rhizobia : the carbohydrate component.

    abstract::The sugar compositions of root hairs of a variety of plant species were determined. Root hairs of legumes had very similar compositions, whereas those of different families varied widely. Only approximately 50% of the weight of the root hairs of legumes could be accounted for by sugar. Up to 80% of the weight of root ...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Mort AJ,Grover PB

    更新日期:1988-02-01 00:00:00