Abstract:
:Accumulation of mRNAs for the defense-related genes phenylalanine ammonia-lyase (PAL), chalcone synthase, chitinase (CHT), glucanase, and hydroxyproline-rich glycoprotein were examined in roots of dark red kidney bean (Phaseolus vulgaris L. cv Moncalm) colonized by the arbuscular mycorrhizal fungus Glomus intraradices Schenck & Smith. In three separate experiments the root length colonized ranged from 28 to 55% by 28 d after planting and inoculation. RNA blot analysis revealed little change in the accumulations of PAL, chalcone synthase, CHT, glucanase, and hydroxyproline-rich glycoprotein transcripts from the 28-d mycorrhizal roots compared to the uninoculated controls. Normalizing the ratios of defense-related transcript accumulation against RNA pools regarded as being constitutively expressed, actin mRNA, 25S rRNA, and 18S rRNA, indicated that changes in the ratios of up to 20% occur according to the RNA pool used for normalization. In situ hybridizations of colonized roots using probes for PAL and CHT showed that accumulations of both transcripts occurred only in arbusculated cells. Both young, finely branched arbuscules and older, clumped arbuscules displayed PAL and CHT message accumulations. The PAL and CHT mRNA accumulations were greater in cortical cells containing young arbuscules than in cells containing clumped arbuscules. Intercellular hyphae and vesicles elicited no response.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Blee KA,Anderson AJdoi
10.1104/pp.110.2.675subject
Has Abstractpub_date
1996-02-01 00:00:00pages
675-688issue
2eissn
0032-0889issn
1532-2548pii
110/2/675journal_volume
110pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::Callus cultures of peanut (Arachis hypogaea L. cv Valencia Tennessee Red) cotyledons grown on Linsmaier and Skoog medium containing normal levels of auxin and cytokinin do not synthesize either 4-methyl-eneglutamic acid or 4-methyleneglutamine, which nonprotein amino acids are normally found in significant amounts in ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.76.1.161
更新日期:1984-09-01 00:00:00
abstract::Genetic improvement of plant architecture is one of the strategies for increasing the yield potential of rice (Oryza sativa). Although great progress has been made in the understanding of plant architecture regulation, the precise mechanism is still an urgent need to be revealed. Here, we report that over-expression o...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.17.00964
更新日期:2018-01-01 00:00:00
abstract::Prolonged growth of cell cultures of sycamore (Acer pseudoplatanus L.) on agar medium containing myo-inositol-2-(3)H resulted in incorporation of label predominately into uronosyl and pentosyl units of cell wall polysaccharides. Procedures normally used to distinguish between pectic substance and hemicellulose yielded...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.41.9.1489
更新日期:1966-11-01 00:00:00
abstract::Light-stimulated carotenoid biosynthesis associated with the transformation of etioplasts to chloroplasts was investigated after dark-grown maize (Zea mays) seedlings were transferred into light. These studies focused on the enzymes of the pathway to detect those enzyme activities that were stimulated in the light and...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.2.529
更新日期:1994-06-01 00:00:00
abstract::Hydrogen-1 nuclear magnetic resonance spectroscopy was used to study water allocation in cell compartments of sun and shade leaves. NMR spectra of Acer platanoides were resolved into two peaks that were assigned to chloroplast and nonchloroplast water. Sun leaves contained 1.7 times more water per unit area of surface...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.86.1.16
更新日期:1988-01-01 00:00:00
abstract::Suspension-cultured cells of sycamore (Acer pseudoplatanus L.) which were immersed in liquid nitrogen after prefreezing to the temperatures from -30 to -50 C in the presence of dimethylsulfoxide and glucose as cryoprotective additive could proliferate vigorously when rewarmed rapidly in water at 40 C. For maintaining ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.54.5.722
更新日期:1974-11-01 00:00:00
abstract::Plants are able to integrate exogenous 1-deoxy-D-xylulose (DX) into the 2C-methyl-D-erythritol 4-phosphate pathway, implicated in the biosynthesis of plastidial isoprenoids. Thus, the carbohydrate needs to be phosphorylated into 1-deoxy-D-xylulose 5-phosphate and translocated into plastids, or vice versa. An enzyme ca...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.086652
更新日期:2006-10-01 00:00:00
abstract::The effect of the proline analog azetidine-2-carboxylic acid (Aze) on the induction and the regulation of heat-shock (HS) mRNA accumulation and heat-shock protein (HSP) synthesis in soybean (Glycine max) seedlings was studied. Treatment with Aze elicited an HS-like response at the normal growth temperature, 28[deg]C, ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.110.1.241
更新日期:1996-01-01 00:00:00
abstract::A novel mutant of Arabidopsis (Arabidopsis thaliana), having highly glossy inflorescence stems, postgenital fusion in floral organs, and reduced fertility, was isolated from an ethyl methanesulfonate-mutagenized population and designated glossyhead1 (gsd1). The gsd1 locus was mapped to chromosome 1, and the causal gen...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.111.185132
更新日期:2011-11-01 00:00:00
abstract::Effects of red and blue light at irradiances from 1.6 to 28.3 micromolar per square meter per second on chloroplast pigments, light-harvesting pigment-proteins associated with photosystem II, and the corresponding mRNA were evaluated in maize (Zea mays L.) plants (OP Golden Bantum) grown for 14 days under 14 hours lig...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.91.1.163
更新日期:1989-09-01 00:00:00
abstract::The presence of multiple forms of alpha-amylase in gibberellic acid-treated embryoless barley half-seeds was demonstrated by separation on diethylaminoethyl-Sephadex and isoelectric focusing polyacrylamide gel disc electrophoresis. Two major alpha-amylase fractions (A and B), each consisting of two to three isozyme co...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.46.4.586
更新日期:1970-10-01 00:00:00
abstract::A new method has been developed for screening algal photosynthetic mutants. This method uses autoradiography to assess gross photosynthetic 14C fixation by green algal colonies on agar plates and allows the identification of clones that differ in photosynthetic characteristics from wild-type cells. Three wild-type cel...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.110.4.1283
更新日期:1996-04-01 00:00:00
abstract::To identify genetic loci involved in the regulation of organ-specific enzyme activities, a specific histochemical staining protocol was used in combination with quantitative trait locus (QTL) analysis. Using phosphoglucomutase (PGM) as an example, it is shown that enzyme activity can specifically, and with high resolu...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.103.027615
更新日期:2004-01-01 00:00:00
abstract::Tumor formation in Vitis species and hybrids, incited by Agrobacterium tumefaciens, was altered by chemical, physical, developmental, and genetic variables. Knowledge of the effect of these variables was used to develop a stringent in vitro assay system to select parents for a study of genetic factors that modulate tu...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.96.1.121
更新日期:1991-05-01 00:00:00
abstract::Amino compounds representative of the major N solutes of xylem sap were pulse-fed (10 to 20 minutes) singly in (14)C-labeled form to cut transpiring shoots of white lupin (Lupinus albus L.). (14)C distribution was studied by autoradiography and radioassays of phloem sap, leaflet tissues, and shoot parts harvested at i...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.63.6.1076
更新日期:1979-06-01 00:00:00
abstract::Poplar (Populus tremula x alba) trees (clone INRA 717-1-B4) were cultivated for 1 month in phytotronic chambers with two different levels of ozone (60 and 120 nL L(-1)). Foliar activities of shikimate dehydrogenase (EC 1.1.1.25), phenylalanine ammonia lyase (EC 4.3.1.5), and cinnamyl alcohol dehydrogenase (CAD, EC 1.1...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.103.031765
更新日期:2004-02-01 00:00:00
abstract::Photosynthetically derived sugar provides carbon skeletons for lipid biosynthesis. We used mutants of Arabidopsis (Arabidopsis thaliana) and the expression of oleogenic factors to investigate relationships among sugar availability, lipid synthesis, and the accumulation of triacylglycerol (TAG) in leaf tissue. The adg1...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.17.00828
更新日期:2017-10-01 00:00:00
abstract::We have purified the protein for the enzyme sucrose phosphate synthase (SPS) from corn (Zea mays) leaves. Partially purified SPS protein was used to generate specific monoclonal antibodies. The following immunoaffinity chromatography allowed the isolation of pure SPS protein. The apparent molecular mass of the SPS pol...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.96.2.473
更新日期:1991-06-01 00:00:00
abstract::The ATPase activity of a membrane fraction from soybean (Glycine max L.) root and callus cells, presumed to be enriched in plasma membrane, has been characterized with respect to ion stimulation, pH requirement, and nucleotide specificity. The enzyme from both sources was activated by divalent cations (Mg(2+) > Mn(2+)...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.59.2.264
更新日期:1977-02-01 00:00:00
abstract::Plant growth and development are strongly dependent on sink-source interactions. In the majority of plants, sucrose (Suc) is the dominant form in which photo-assimilate is transported from source to sinks. Although the effects of Suc on photosynthetic metabolism have been intensively studied, the effect of Suc supply ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.103.024802
更新日期:2003-10-01 00:00:00
abstract::A full-genome microarray of the (oxy)photosynthetic cyanobacterium Synechocystis sp. PCC 6803 was used to identify genes that were transcriptionally regulated by growth in iron (Fe)-deficient versus Fe-sufficient media. Transcript accumulation for 3,165 genes in the genome was analyzed using an analysis of variance mo...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.103.024018
更新日期:2003-08-01 00:00:00
abstract::Biochemical and electrophysiological studies on plant vacuolar transporters became feasible in the late 1970s and early 1980s, when methods to isolate large quantities of intact vacuoles and purified vacuolar membrane vesicles were established. However, with the exception of the H+-ATPase and H+-PPase, which could be ...
journal_title:Plant physiology
pub_type: 杂志文章,评审
doi:10.1104/pp.17.01481
更新日期:2018-02-01 00:00:00
abstract::Brassinosteroids (BRs) are a class of steroid hormones regulating multiple aspects of plant growth, development, and adaptation. Compared with extensive studies in Arabidopsis (Arabidopsis thaliana), the mechanism of BR signaling in rice (Oryza sativa) is less understood. Here, we identified OsWRKY53, a transcription ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.17.00946
更新日期:2017-11-01 00:00:00
abstract::Induction of ferric reductase activity in dicots and nongrass monocots is a well-recognized response to Fe deficiency. Recent evidence has shown that Cu deficiency also induces plasma membrane Fe reduction. In this study we investigated whether other nutrient deficiencies could also induce ferric reductase activity in...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.114.3.1061
更新日期:1997-07-01 00:00:00
abstract::An NADP-dependent dehydrogenase catalyzing the conversion of l-sorbosone to l-ascorbic acid has been isolated from Phaseolus vulgaris L. and Spinacia oleracea L. and partially purified. It is stable at -20 degrees C for up to 8 months. Molecular masses, as determined by gel filtration, were 21 and 29 kilodaltons for b...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.94.3.1492
更新日期:1990-11-01 00:00:00
abstract::Increasing concentrations of K (20, 200, 2000 mum) in the nutrient solution depressed Ca content and concentration in barley plants growing in nutrient solutions of low Ca concentrations (250 and 2500 mum). Increasing K from 20 to 200 mum depressed Ca absorption more than increasing K from 200 to 2000 mum K.The strong...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.43.10.1717
更新日期:1968-10-01 00:00:00
abstract::In climates that experience short growing seasons due to drought, heat, or end-of-season frost, early flowering is a highly desirable trait for chickpea (Cicer arietinum). In this study, we mapped, sequenced, and characterized Early flowering3 (Efl3), an ortholog of Arabidopsis (Arabidopsis thaliana) EARLY FLOWERING3 ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.17.00082
更新日期:2017-10-01 00:00:00
abstract::The possibility that photosynthetic competence is gratuitous for light-induced chloroplast development in Euglena gracilis var. bacillaris was examined by incubating dark-grown resting cells in the light with DCMU, an inhibitor of photosynthesis. Under these conditions photosynthetic carbon dioxide fixation was inhibi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.42.12.1716
更新日期:1967-12-01 00:00:00
abstract::Plants coordinate the expression of photosynthesis-related genes in response to growth and environmental changes. In species that conduct two-cell C4 photosynthesis, expression of photosynthesis genes is partitioned such that leaf mesophyll and bundle sheath cells accumulate different components of the photosynthetic ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.19.01053
更新日期:2020-03-01 00:00:00
abstract::Incubation of freshly cut carrot tissue in Na(3)ADP and Na(3)ATP promotes a marked intracellular increase in both ADP and ATP. The rapid increase in ATP during an ADP incubation and in ADP during an ATP incubation results from the activity of cytoplasmic enzyme systems upon the nucleotide absorbed into the cell from t...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.45.4.500
更新日期:1970-04-01 00:00:00