Abstract:
:The enormous diversity of seed traits is an intriguing feature and critical for the overwhelming success of higher plants. In particular, seed mass is generally regarded to be key for seedling development but is mostly approximated by using scanning methods delivering only two-dimensional data, often termed seed size. However, three-dimensional traits, such as the volume or mass of single seeds, are very rarely determined in routine measurements. Here, we introduce a device named phenoSeeder, which enables the handling and phenotyping of individual seeds of very different sizes. The system consists of a pick-and-place robot and a modular setup of sensors that can be versatilely extended. Basic biometric traits detected for individual seeds are two-dimensional data from projections, three-dimensional data from volumetric measures, and mass, from which seed density is also calculated. Each seed is tracked by an identifier and, after phenotyping, can be planted, sorted, or individually stored for further evaluation or processing (e.g. in routine seed-to-plant tracking pipelines). By investigating seeds of Arabidopsis (Arabidopsis thaliana), rapeseed (Brassica napus), and barley (Hordeum vulgare), we observed that, even for apparently round-shaped seeds of rapeseed, correlations between the projected area and the mass of seeds were much weaker than between volume and mass. This indicates that simple projections may not deliver good proxies for seed mass. Although throughput is limited, we expect that automated seed phenotyping on a single-seed basis can contribute valuable information for applications in a wide range of wild or crop species, including seed classification, seed sorting, and assessment of seed quality.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Jahnke S,Roussel J,Hombach T,Kochs J,Fischbach A,Huber G,Scharr Hdoi
10.1104/pp.16.01122subject
Has Abstractpub_date
2016-11-01 00:00:00pages
1358-1370issue
3eissn
0032-0889issn
1532-2548pii
pp.16.01122journal_volume
172pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::An acetylcholine hydrolyzing enzyme was prepared and purified (40 times) from dwarf bean hypocotyl hooks. The purity of the enzyme was proved by polyacrylamide gel electrophoresis. The molecular weight of the enzyme was determined to be 65,000 daltons. Enzyme activity was the highest at pH 8.0 and between 30 and 36 C....
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.65.3.447
更新日期:1980-03-01 00:00:00
abstract::The swelling of potato (Solanum tuberosum L.) mitochondria in isosmotic ammonium salts of phosphate, chloride, malate, succinate, and citrate was investigated by measuring light scattering. Potato mitochondria swell spontaneously in ammonium phosphate, and this swelling can be inhibited in N-ethylmaleimide. They swell...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.51.4.667
更新日期:1973-04-01 00:00:00
abstract::Circadian clocks drive rhythms with a period near 24 h, but the molecular basis of the regulation of the period of the circadian clockis poorly understood. We previously demonstrated that metabolites affect the free-running period of the circadian oscillator of Arabidopsis (Arabidopsis thaliana), with endogenous sugar...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.18.00571
更新日期:2018-09-01 00:00:00
abstract::Lipoxidase, in the cotyledons of squash (Cucurbita moscata) seedlings grown in the dark, reached its peak activity on the fifth day and then declined to its lowest activity on the eighth day. Under continuous irradiation, the rate of enzyme disappearance was accelerated by red (655 mmu) and was retarded by far-red (73...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.42.3.421
更新日期:1967-03-01 00:00:00
abstract::Peroxisomes are single membrane-delimited subcellular organelles that carry out numerous vital metabolic reactions in nearly all eukaryotes. Peroxisomes alter their morphology, abundance, and enzymatic constituents in response to environmental cues, yet little is known about the underlying mechanisms. In this work, we...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.107.113555
更新日期:2008-03-01 00:00:00
abstract::The construction, operation, and effectiveness of an abscission measuring instrument called an abscissor is described. The device measured the force required for a spring-opposed plunger to shear abscission zone explants and was capable of automatically recording break strength data. Examples of data obtained with the...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.44.8.1139
更新日期:1969-08-01 00:00:00
abstract::[(14)C]butyl gallate was used in binding studies to investigate the cyanide-resistant respiratory pathway in mitochondria isolated from a variety of sources displaying varying levels of cyanide resistance. Highly cyanide-resistant mitochondria were isolated from aroid spadices, while moderately cyanide-resistant mitoc...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.80.1.196
更新日期:1986-01-01 00:00:00
abstract::Cesium (Cs) is chemically similar to potassium (K). However, although K is an essential element, Cs is toxic to plants. Two contrasting hypotheses to explain Cs toxicity have been proposed: (1) extracellular Cs+ prevents K+ uptake and, thereby, induces K starvation; and (2) intracellular Cs+ interacts with vital K(+)-...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.046672
更新日期:2004-11-01 00:00:00
abstract::Under normal field growth conditions, canola (Brassica napus) seeds produce chloroplasts during early seed development and then catabolize the photosynthetic machinery during seed maturation, producing mature seeds at harvest that are essentially free of chlorophyll (Chl). However, frost exposure early in canola seed ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.084483
更新日期:2006-09-01 00:00:00
abstract::We have characterized two related cDNAs (RCI2A and RCI2B) corresponding to genes from Arabidopsis thaliana, the expression of which is transiently induced by low, nonfreezing temperatures. RCI2A and RCI2B encode small (54 amino acids), highly hydrophobic proteins that bear two potential transmembrane domains. They sho...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.115.2.569
更新日期:1997-10-01 00:00:00
abstract::Root respiration associated with symbiotic fixation in soybean (Glycine max [L.] Merr.) was estimated by four methods.Averaged over the life of the plant, the root respires 5.8 milligrams C per milligram N accumulated from fixation. When nitrogenase (C(2)H(2)) activity and root respiration were decreased by treating r...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.72.3.701
更新日期:1983-07-01 00:00:00
abstract::Levels of the enzymes that produce wound response mediators have to be controlled tightly in unwounded tissues. The Arabidopsis (Arabidopsis thaliana) fatty acid oxygenation up-regulated8 (fou8) mutant catalyzes high rates of alpha -linolenic acid oxygenation and has higher than wild-type levels of the alpha -linoleni...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.150474
更新日期:2010-03-01 00:00:00
abstract::The EGG CELL1 (EC1) gene family of Arabidopsis (Arabidopsis thaliana) comprises five members that are specifically expressed in the egg cell and redundantly control gamete fusion during double fertilization. We investigated the activity of all five EC1 promoters in promoter-deletion studies and identified SUF4 (SUPPRE...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.16.01024
更新日期:2017-01-01 00:00:00
abstract::Blue light induces the differentiation of Chlamydomonas reinhardtii pregametes to gametes. The light-induced conversion of pregametes to gametes is protein synthesis dependent and proceeds only after a lag phase. Upon incubation in the dark, gametes lost their mating ability, resulting in dark-inactivated gametes. Rei...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.115.3.1241
更新日期:1997-11-01 00:00:00
abstract::Sporopollenin is the major component of the outer pollen wall (exine). Fatty acid derivatives and phenolics are thought to be its monomeric building blocks, but the precise structure, biosynthetic route, and genetics of sporopollenin are poorly understood. Based on a phenotypic mutant screen in Arabidopsis (Arabidopsi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.144469
更新日期:2009-10-01 00:00:00
abstract::In apple (Malus domestica Borkh.) sorbitol is the primary product of photosynthesis, the major translocated form of carbon, and a common fruit constituent and storage compound. Previous work on sorbitol metabolism has revealed a NADPH-dependent aldose 6-phosphate reductase (A6PR) in green tissues, and a NAD-dependent ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.70.2.335
更新日期:1982-08-01 00:00:00
abstract::Understanding how plants sense and respond to changes in nitrogen availability is the first step toward developing strategies for biotechnological applications, such as improvement of nitrogen use efficiency. However, components involved in nitrogen signaling pathways remain poorly characterized. Calcium is a second m...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.15.00961
更新日期:2015-10-01 00:00:00
abstract::The major light-harvesting chlorophyll a/b binding protein (LHCP) of higher plant chloroplasts is nuclear-encoded, synthesized as a precursor, and processed upon import. We have previously (GK Lamppa, M Abad [1987] J Cell Biol 105: 2641-2648) identified a soluble enzyme that cleaves the LHCP precursor (pLHCP). In this...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.90.1.117
更新日期:1989-05-01 00:00:00
abstract::Plant development requires regulation of both cell division and differentiation. The class 1 KNOTTED1-like homeobox (KNOX) genes such as knotted1 (kn1) in maize (Zea mays) and SHOOTMERISTEMLESS in Arabidopsis (Arabidopsis thaliana) play a role in maintaining shoot apical meristem indeterminacy, and their misexpression...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.076075
更新日期:2006-08-01 00:00:00
abstract::Potato MADS box 1 (POTM1) is a member of the SQUAMOSA-like family of plant MADS box genes isolated from an early stage tuber cDNA library. The RNA of POTM1 is most abundant in vegetative meristems of potato (Solanum tuberosum), accumulating specifically in the tunica and corpus layers of the meristem, the procambium, ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.102.012500
更新日期:2003-04-01 00:00:00
abstract::Pathogen-associated molecular patterns (PAMPs) trigger plant defenses when perceived by surface-localized immune receptors. PAMP-triggered immunity (PTI) plays a vital role in the resistance of plants to numerous potential pathogens. MicroRNA (miRNA) biogenesis is known to be important for PTI, but miRNA species invol...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.151803
更新日期:2010-04-01 00:00:00
abstract::The cultivated tomato (Lycopersicon esculentum) has a unipinnate compound leaf. In the developing leaf primordium, major leaflet initiation is basipetal, and lobe formation and early vascular differentiation are acropetal. We show that engineered alterations in the expression of a tomato homeobox gene, LeT6, can cause...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.117.3.771
更新日期:1998-07-01 00:00:00
abstract::Young cabbage plants (Brassica oleracea L. var. capitata) that were exposed to an atmosphere at 50% relative humidity transpired freely and accumulated significant quantities of (45)Ca in the leaves. Plants that were enclosed by plastic bags to stop transpiration from all leaves exhibited guttation with the developmen...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.60.6.854
更新日期:1977-12-01 00:00:00
abstract::The flow of sap in Populus tremuloides was studied by the thermoelectric method immediately prior to and at several periods of time after radical girdling. Similarly, the flow of sap in Betula papyrifera was studied before and after girdling or injection with metabolic inhibitors or a filtrate of a culture of Ceratocy...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.41.4.641
更新日期:1966-04-01 00:00:00
abstract::The phospholipid dependence of the UDP-glucose sterol glucosyl transferase (UDPG-SGTase) from maize coleoptiles was previously demonstrated using the partially purified and highly delipidated enzyme, in the presence of the detergent Triton X-100 (P Ullmann, P Bouvier-Navé, P Benveniste [1987] Plant Physiol 85: 51-55)....
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.91.2.567
更新日期:1989-10-01 00:00:00
abstract::The JASMONATE ZIM DOMAIN (JAZ) proteins function as negative regulators of jasmonic acid signaling in plants. We cloned 12 JAZ genes from native tobacco (Nicotiana attenuata), including nine novel JAZs in tobacco, and examined their expression in plants that had leaves elicited by wounding or simulated herbivory. Most...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.193771
更新日期:2012-06-01 00:00:00
abstract::High Na(+) concentrations may disrupt K(+) and Ca(2+) transport and interfere with growth of many plant species, cotton (Gossypium hirsutum L.) included. Elevated Ca(2+) levels often counteract these consequences of salinity. The effect of supplemental Ca(2+) on influx of Ca(2+), K(+), and Na(+) in roots of intact, sa...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.83.3.510
更新日期:1987-03-01 00:00:00
abstract::An experiment was conducted to investigate the reduction of endogenous NO(3) (-), which had been taken up by plants in darkness, during the course of the subsequent light period. Vegetative, nonnodulated soybean plants (Glycine max [L]. Merrill, ;Ransom') were exposed to 1.0 millimolar (15)NO(3) (-) for 12 hours in da...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.84.4.1421
更新日期:1987-08-01 00:00:00
abstract::Sucrose gradient profiles of polyribosomes from the coleoptilar node region of seedlings of Zea mays L. were obtained without pelleting and redispersion of the particles. Water stress caused a shift of ribosomes from the polymeric to the monomeric form, starting about 30 minutes after stress initiation and when the wa...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.46.2.281
更新日期:1970-08-01 00:00:00
abstract::Experiments were conducted in growth chambers to examine the effect of a mild water stress (-200 kilopascals polyethylene glycol) on frond elongation and water status of the ostrich fern (Matteuccia struthiopteris [L.] Todaro). Measurements were taken for two days, starting one day after the application of polyethylen...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.72.1.96
更新日期:1983-05-01 00:00:00