The culture of plant cells with ammonium salts as the sole nitrogen source.

Abstract:

:Soybean cell suspension cultures grew on defined media with ammonium as the sole nitrogen source if Krebs cycle acids were added. Satisfactory growth was obtained with ammonium salts of citrate, malate, fumarate, or succinate, when compared with the regular medium containing nitrate and ammonium. Little or no growth occurred when ammonium salts of shikimate, tartrate, acetate, carbonate, or sulfate were used. The cells also grew well with l-glutamine as nitrogen source. The specific activities of glutamine synthetase and isocitrate dehydrogenase (nicotinamide adenine dinucleotide phosphate) were lower than in cells grown on a nitrate medium, but ammonium enhanced the activity of glutamate dehydrogenase. Cells of soybean, wheat, and flax have been cultured for an extended period on the ammonium citrate medium.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Gamborg OL,Shyluk JP

doi

10.1104/pp.45.5.598

subject

Has Abstract

pub_date

1970-05-01 00:00:00

pages

598-600

issue

5

eissn

0032-0889

issn

1532-2548

journal_volume

45

pub_type

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