Abstract:
:Soybean cell suspension cultures grew on defined media with ammonium as the sole nitrogen source if Krebs cycle acids were added. Satisfactory growth was obtained with ammonium salts of citrate, malate, fumarate, or succinate, when compared with the regular medium containing nitrate and ammonium. Little or no growth occurred when ammonium salts of shikimate, tartrate, acetate, carbonate, or sulfate were used. The cells also grew well with l-glutamine as nitrogen source. The specific activities of glutamine synthetase and isocitrate dehydrogenase (nicotinamide adenine dinucleotide phosphate) were lower than in cells grown on a nitrate medium, but ammonium enhanced the activity of glutamate dehydrogenase. Cells of soybean, wheat, and flax have been cultured for an extended period on the ammonium citrate medium.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Gamborg OL,Shyluk JPdoi
10.1104/pp.45.5.598subject
Has Abstractpub_date
1970-05-01 00:00:00pages
598-600issue
5eissn
0032-0889issn
1532-2548journal_volume
45pub_type
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