MIKC* MADS-protein complexes bind motifs enriched in the proximal region of late pollen-specific Arabidopsis promoters.

Abstract:

:The genome of Arabidopsis (Arabidopsis thaliana) encodes over 100 MADS-domain transcription factors, categorized into five phylogenetic subgroups. Most research efforts have focused on just one of these subgroups (MIKC(c)), whereas the other four remain largely unexplored. Here, we report on five members of the so-called Mdelta or Arabidopsis MIKC* (AtMIKC*) subgroup, which are predominantly expressed during the late stages of pollen development. Very few MADS-box genes function in mature pollen, and from this perspective, the AtMIKC* genes are therefore highly exceptional. We found that the AtMIKC* proteins are able to form multiple heterodimeric complexes in planta, and that these protein complexes exhibit a for the MADS-family unusual and high DNA binding specificity in vitro. Compared to their occurrence in promoters genome wide, AtMIKC* binding sites are strongly overrepresented in the proximal region of late pollen-specific promoters. By combining our experimental data with in silico genomics and pollen transcriptomics approaches, we identified a considerable number of putative direct target genes of the AtMIKC* transcription factor complexes in pollen, many of which have known or proposed functions in pollen tube growth. The expression of several of these predicted targets is altered in mutant pollen in which all AtMIKC* complexes are affected, and in vitro germination of this mutant pollen is severely impaired. Our data therefore suggest that the AtMIKC* protein complexes play an essential role in transcriptional regulation during late pollen development.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Verelst W,Saedler H,Münster T

doi

10.1104/pp.106.089805

subject

Has Abstract

pub_date

2007-01-01 00:00:00

pages

447-60

issue

1

eissn

0032-0889

issn

1532-2548

pii

pp.106.089805

journal_volume

143

pub_type

杂志文章
  • Modulation of oat mitochondrial ATPase activity by CA2+ and phytochrome.

    abstract::The activity of a Mg(2+)-dependent ATPase present in highly purified preparations of Avena mitochondria was photoreversibly modulated by red/far-red light treatments. These results were obtained either with mitochondria isolated from plants irradiated with white light prior to the extraction or with mitochondria isol...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.74.4.827

    authors: Serlin BS,Sopory SK,Roux SJ

    更新日期:1984-04-01 00:00:00

  • Phosphate Treatment Strongly Inhibits New Arbuscule Development But Not the Maintenance of Arbuscule in Mycorrhizal Rice Roots.

    abstract::Phosphorus (P) is a crucial nutrient for plant growth, but its availability to roots is limited in soil. Arbuscular mycorrhizal (AM) symbiosis is a promising strategy for improving plant P acquisition. However, P fertilizer reduces fungal colonization (P inhibition) and compromises mycorrhizal P uptake, warranting stu...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.00127

    authors: Kobae Y,Ohmori Y,Saito C,Yano K,Ohtomo R,Fujiwara T

    更新日期:2016-05-01 00:00:00

  • Sequential changes in the lipids of developing proplastids isolated from green maize leaves.

    abstract::Changes in lipid composition were followed as a proplastid develops into a chloroplast. Methods were devised for the isolation of developing proplastids from sections of five different ages from the same 7-day-old maize (Zea mays var. Kelvedon Glory) leaf. Electron micrographs illustrate the homogeneity of the five ty...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.57.5.789

    authors: Leese BM,Leech RM

    更新日期:1976-05-01 00:00:00

  • Sucrose phosphate synthase and sucrose accumulation at low temperature.

    abstract::The influence of growth temperature on the free sugar and sucrose phosphate synthase content and activity of spinach (Spinacia oleracea) leaf tissue was studied. When plants were grown at 25 degrees C for 3 weeks and then transferred to a constant 5 degrees C, sucrose, glucose, and fructose accumulated to high levels ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.100.1.502

    authors: Guy CL,Huber JL,Huber SC

    更新日期:1992-09-01 00:00:00

  • Fourier transform infrared microspectroscopy detects changes in protein secondary structure associated with desiccation tolerance in developing maize embryos

    abstract::Isolated immature maize (Zea mays L.) embryos have been shown to acquire tolerance to rapid drying between 22 and 25 d after pollination (DAP) and to slow drying from 18 DAP onward. To investigate adaptations in protein profile in association with the acquisition of desiccation tolerance in isolated, immature maize em...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.116.3.1169

    authors: Wolkers WF,Bochicchio A,Selvaggi G,Hoekstra FA

    更新日期:1998-03-01 00:00:00

  • Natural H Currents Traverse Growing Roots and Root Hairs of Barley (Hordeum vulgare L.).

    abstract::With the aid of an extracellular vibrating electrode, natural electric fields were detected and measured in the medium near growing roots and root hairs of barley seedlings. An exploration of these fields indicates that both the root as a whole, as well as individual root hairs, drive large steady currents through the...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.64.3.512

    authors: Weisenseel MH,Dorn A,Jaffe LF

    更新日期:1979-09-01 00:00:00

  • Regulation of light-harvesting chlorophyll protein biosynthesis in greening seedlings : a species comparison.

    abstract::The biosynthesis of the chlorophyll a/b binding protein associated with photosystem II (LHC-II) was characterized during light-induced greening of etiolated barley (Hordeum vulgare [L.] cv Boone), maize (Zea mays [L.] Pioneer 3148), pea (Pisum sativum [L.] cv Progress 9), and soybean (Glycine max [L.] Merr. cv Ransom ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.85.4.971

    authors: Mathis JN,Burkey KO

    更新日期:1987-12-01 00:00:00

  • Galactinol synthase1. A novel heat shock factor target gene responsible for heat-induced synthesis of raffinose family oligosaccharides in Arabidopsis.

    abstract::Heat shock factors (HSFs) are transcriptional regulators of the heat shock response. The major target of HSFs are the genes encoding heat shock proteins (HSPs), which are known to have a protective function that counteracts cytotoxic effects. To identify other HSF target genes, which may be important determinants for ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.042606

    authors: Panikulangara TJ,Eggers-Schumacher G,Wunderlich M,Stransky H,Schöffl F

    更新日期:2004-10-01 00:00:00

  • Plant Chemical Genetics: From Phenotype-Based Screens to Synthetic Biology.

    abstract::The treatment of a biological system with small molecules to specifically perturb cellular functions is commonly referred to as chemical biology. Small molecules are used commercially as drugs, herbicides, and fungicides in different systems, but in recent years they are increasingly exploited as tools for basic resea...

    journal_title:Plant physiology

    pub_type: 杂志文章,评审

    doi:10.1104/pp.16.01805

    authors: Dejonghe W,Russinova E

    更新日期:2017-05-01 00:00:00

  • Chlorophyll synthesis in chlorella I. Occurrence of a lag phase on initiation of a dilute culture.

    abstract::A culture of Chlorella established by 30-fold dilution of a culture already grown to a level of 15 ml packed cell volume per liter produces little chlorophyll for approximately 12 hours. Investigation of other characteristics such as nitrogen incorporation, increase in packed cell volume and dry weight as well as RNA ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.41.10.1695

    authors: Shugarman PM,Appleman D

    更新日期:1966-12-01 00:00:00

  • Sucrose phosphate synthase, a key enzyme for sucrose biosynthesis in plants: protein purification from corn leaves and immunological detection.

    abstract::We have purified the protein for the enzyme sucrose phosphate synthase (SPS) from corn (Zea mays) leaves. Partially purified SPS protein was used to generate specific monoclonal antibodies. The following immunoaffinity chromatography allowed the isolation of pure SPS protein. The apparent molecular mass of the SPS pol...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.96.2.473

    authors: Bruneau JM,Worrell AC,Cambou B,Lando D,Voelker TA

    更新日期:1991-06-01 00:00:00

  • Genetic Interactions between PEROXIN12 and Other Peroxisome-Associated Ubiquitination Components.

    abstract::Most eukaryotic cells require peroxisomes, organelles housing fatty acid β-oxidation and other critical metabolic reactions. Peroxisomal matrix proteins carry peroxisome-targeting signals that are recognized by one of two receptors, PEX5 or PEX7, in the cytosol. After delivering the matrix proteins to the organelle, t...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.01211

    authors: Kao YT,Fleming WA,Ventura MJ,Bartel B

    更新日期:2016-11-01 00:00:00

  • Involvement of Abscisic Acid in Ethylene-Induced Cotyledon Abscission in Cotton Seedlings.

    abstract::Cotton (Gossypium hirsutum L. cv LG102) seedlings raised from seeds exposed to 100 [mu]M norflurazon (NFZ) during imbibition contained reduced levels of free abscisic acid (ABA) and were visibly achlorophyllous. Exposure of untreated cotton seedlings to ethylene concentrations >1 [mu]L/L for 24 h resulted in cotyledon...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.101.2.641

    authors: Suttle JC,Hultstrand JF

    更新日期:1993-02-01 00:00:00

  • Replication of nonautonomous retroelements in soybean appears to be both recent and common.

    abstract::Retrotransposons and their remnants often constitute more than 50% of higher plant genomes. Although extensively studied in monocot crops such as maize (Zea mays) and rice (Oryza sativa), the impact of retrotransposons on dicot crop genomes is not well documented. Here, we present an analysis of retrotransposons in so...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.127910

    authors: Wawrzynski A,Ashfield T,Chen NW,Mammadov J,Nguyen A,Podicheti R,Cannon SB,Thareau V,Ameline-Torregrosa C,Cannon E,Chacko B,Couloux A,Dalwani A,Denny R,Deshpande S,Egan AN,Glover N,Howell S,Ilut D,Lai H,Del Campo S

    更新日期:2008-12-01 00:00:00

  • Absorption and distribution of high specific radioactivity 2-C-abscisic Acid in cotton seedlings.

    abstract::High specific radioactivity (26.3 mc/mmole) racemic 2-(14)C-abscisic acid was synthesized. An aliquot of abscisic acid, 1.2 x 10(-4)m in aqueous methanolic solution, was applied to the surface of either a cotyledon or the first true leaf of 8- to 32-day-old cotton seedlings (Gossypium hirsutum L.). After various inter...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.52.5.443

    authors: Shindy WW,Asmundson CM,Smith OE,Kumamoto J

    更新日期:1973-11-01 00:00:00

  • Plasma Membrane-Associated Receptor-like Kinases Relocalize to Plasmodesmata in Response to Osmotic Stress.

    abstract::Plasmodesmata act as key elements in intercellular communication, coordinating processes related to plant growth, development, and responses to environmental stresses. While many of the developmental, biotic, and abiotic signals are primarily perceived at the plasma membrane (PM) by receptor proteins, plasmodesmata al...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.19.00473

    authors: Grison MS,Kirk P,Brault ML,Wu XN,Schulze WX,Benitez-Alfonso Y,Immel F,Bayer EM

    更新日期:2019-09-01 00:00:00

  • The culture of plant cells with ammonium salts as the sole nitrogen source.

    abstract::Soybean cell suspension cultures grew on defined media with ammonium as the sole nitrogen source if Krebs cycle acids were added. Satisfactory growth was obtained with ammonium salts of citrate, malate, fumarate, or succinate, when compared with the regular medium containing nitrate and ammonium. Little or no growth o...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.45.5.598

    authors: Gamborg OL,Shyluk JP

    更新日期:1970-05-01 00:00:00

  • Negative regulation in the expression of a sugar-inducible gene in Arabidopsis thaliana. A recessive mutation causing enhanced expression of a gene for beta-amylase.

    abstract::Expression of a beta-amylase gene of Arabidopsis thaliana (AT beta-Amy) is regulated by sugars. We identified a mutant, hba1, in which the level of expression of AT beta-Amy in leaves of plants that had been grown in a medium with 2% sucrose was significantly higher than that in wild-type plants. Higher that wild-type...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.114.2.575

    authors: Mita S,Hirano H,Nakamura K

    更新日期:1997-06-01 00:00:00

  • The rapid isolation of vacuoles from leaves of crassulacean Acid metabolism plants.

    abstract::A technique is presented for the isolation of vacuoles from Sedum telephium L. leaves. Leaf material is digested enzymically to produce protoplasts rapidly which are partially lysed by gentle osmotic shock and the inclusion of 5 millimolar ethyleneglycol-bis (beta-aminoethyl ether)N,N'-tetraacetic acid in the wash med...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.66.3.379

    authors: Kringstad R,Kenyon WH,Black CC

    更新日期:1980-09-01 00:00:00

  • Triphosphate Tunnel Metalloenzyme Function in Senescence Highlights a Biological Diversification of This Protein Superfamily.

    abstract::The triphosphate tunnel metalloenzyme (TTM) superfamily comprises a group of enzymes that hydrolyze organophosphate substrates. They exist in all domains of life, yet the biological role of most family members is unclear. Arabidopsis (Arabidopsis thaliana) encodes three TTM genes. We have previously reported that AtTT...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.17.00700

    authors: Ung H,Karia P,Ebine K,Ueda T,Yoshioka K,Moeder W

    更新日期:2017-09-01 00:00:00

  • Impact of altered gibberellin metabolism on biomass accumulation, lignin biosynthesis, and photosynthesis in transgenic tobacco plants.

    abstract::Gibberellins (GAs) are involved in regulation of many aspects during plant development. To investigate the impact of altered GA levels on plant growth and metabolism, transgenic tobacco (Nicotiana tabacum) plants have been engineered to express either a GA20-oxidase (AtGA20-ox) or a GA2-oxidase (AtGA2-ox) gene from Ar...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.036988

    authors: Biemelt S,Tschiersch H,Sonnewald U

    更新日期:2004-05-01 00:00:00

  • Localization of [gamma]-Glutamylcysteine Synthetase and Glutathione Synthetase Activity in Maize Seedlings.

    abstract::Fresh weight, protein, cysteine, [gamma]-glutamylcysteine, glutathione, and the extractable activity of the enzymes of glutathione biosynthesis, [gamma]-glutamylcysteine synthetase (EC 6.3.2.2) and glutathione synthetase (EC 6.3.2.3), were measured in roots, scutella, endosperms, and shoots of 3-, 7-, and 11-d-old mai...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.101.2.561

    authors: Ruegsegger A,Brunold C

    更新日期:1993-02-01 00:00:00

  • Pectin Modification in Cell Walls of Ripening Tomatoes Occurs in Distinct Domains.

    abstract::The class of cell wall polysaccharides that undergoes the most extensive modification during tomato (Lycopersicon esculentum) fruit ripening is pectin. De-esterification of the polygalacturonic acid backbone by pectin methylesterase facilitates the depolymerization of pectins by polygalacturonase II (PGII). To investi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.114.1.373

    authors: Steele NM,McCann MC,Roberts K

    更新日期:1997-05-01 00:00:00

  • StGCPRP, a potato gene strongly expressed in stomatal guard cells, defines a novel type of repetitive proline-rich proteins.

    abstract::Guard cells represent a highly differentiated cell type within the epidermis of plant leaves and stems. They respond to many endogenous and environmental signals and thereby modify the size of the stomatal pore they surround. We identified a novel gene that is highly expressed in guard cells of potato (Solanum tuberos...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.122.3.677

    authors: Menke U,Renault N,Mueller-Roeber B

    更新日期:2000-03-01 00:00:00

  • NPR genes, which are negatively regulated by phytochrome action in Lemna gibba L. G-3, can also be positively regulated by abscisic acid.

    abstract::We have found that NPR1 and NPR2, two genes from Lemna gibba L. G-3 that can be negatively regulated by phytochrome action, can also be positively regulated by the plant hormone abscisic acid (ABA). Both genes were responsive to low concentrations of exogenous ABA; an increase in NPR1 RNA could be detected in response...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.3.949

    authors: Williams SA,Weatherwax SC,Bray EA,Tobin EM

    更新日期:1994-07-01 00:00:00

  • Overexpression of ca1pase Decreases Rubisco Abundance and Grain Yield in Wheat.

    abstract::Rubisco catalyzes the fixation of CO2 into organic compounds that are used for plant growth and the production of agricultural products, and specific sugar-phosphate derivatives bind tightly to the active sites of Rubisco, locking the enzyme in a catalytically inactive conformation. 2-carboxy-d-arabinitol-1-phosphate ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.19.00693

    authors: Lobo AKM,Orr DJ,Gutierrez MO,Andralojc PJ,Sparks C,Parry MAJ,Carmo-Silva E

    更新日期:2019-10-01 00:00:00

  • Analysis of curated and predicted plastid subproteomes of Arabidopsis. Subcellular compartmentalization leads to distinctive proteome properties.

    abstract::Carefully curated proteomes of the inner envelope membrane, the thylakoid membrane, and the thylakoid lumen of chloroplasts from Arabidopsis were assembled based on published, well-documented localizations. These curated proteomes were evaluated for distribution of physical-chemical parameters, with the goal of extrac...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.040717

    authors: Sun Q,Emanuelsson O,van Wijk KJ

    更新日期:2004-06-01 00:00:00

  • Physiological characterization of two genes for Na+ exclusion in durum wheat, Nax1 and Nax2.

    abstract::Durum wheat (Triticum turgidum L. subsp. durum Desf.) Line 149 contains two novel major genes for excluding Na(+) from leaf blades, named Nax1 and Nax2. The genes were separated into families containing a single gene and near-isogenic homozygous lines were selected. Lines containing either Nax1 or Nax2 had lower rates...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.086538

    authors: James RA,Davenport RJ,Munns R

    更新日期:2006-12-01 00:00:00

  • Microarray analysis of the genome-wide response to iron deficiency and iron reconstitution in the cyanobacterium Synechocystis sp. PCC 6803.

    abstract::A full-genome microarray of the (oxy)photosynthetic cyanobacterium Synechocystis sp. PCC 6803 was used to identify genes that were transcriptionally regulated by growth in iron (Fe)-deficient versus Fe-sufficient media. Transcript accumulation for 3,165 genes in the genome was analyzed using an analysis of variance mo...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.024018

    authors: Singh AK,McIntyre LM,Sherman LA

    更新日期:2003-08-01 00:00:00

  • Plant body weight-induced secondary growth in Arabidopsis and its transcription phenotype revealed by whole-transcriptome profiling.

    abstract::Wood is an important raw material and environmentally cost-effective renewable source of energy. However, the molecular biology of wood formation (i.e. secondary growth) is surprisingly understudied. A novel experimental system was employed to study the molecular regulation of secondary xylem formation in Arabidopsis....

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.038844

    authors: Ko JH,Han KH,Park S,Yang J

    更新日期:2004-06-01 00:00:00