Transfer of oligosaccharide to protein from a lipid intermediate in plants.

Abstract:

:A lipid-bound oligosaccharide was isolated from pea (Pisum sativum) cotyledons incubated with [(14)C]mannose. The oligosaccharide moiety appeared to be identical with the one obtained from rat liver, known to contain three glucoses, nine mannoses, and two N-acetylglucosamines, and to be involved in protein glycosylation.Enzymes obtained from soya (Glycine max) roots and developing pea cotyledons were found to catalyze the transfer of oligosaccharide from the lipid intermediate to endogenous protein. The enzymes require Mn(2+) and detergent for activity. Evidence is presented indicating that the lipid-bound oligosaccharide with three glucoses is transferred faster than that with less. Some of the peripheral mannoses could be removed without affecting the rate of transfer.The protein-bound oligosaccharide, formed by incubation of whole cotyledons or by transfer with the enzyme preparation, could be released by protease and endo-beta-N-acetylglucosaminidase treatment, as expected for an asparagine-bound high mannose oligosaccharide.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Staneloni RJ,Tolmasky ME,Petriella C,Leloir LF

doi

10.1104/pp.68.5.1175

subject

Has Abstract

pub_date

1981-11-01 00:00:00

pages

1175-9

issue

5

eissn

0032-0889

issn

1532-2548

journal_volume

68

pub_type

杂志文章
  • The role of PENNYWISE and POUND-FOOLISH in the maintenance of the shoot apical meristem in Arabidopsis.

    abstract::Growth of the aerial part of the plant is dependent upon the maintenance of the shoot apical meristem (SAM). A balance between the self-renewing stem cells in the central zone (CZ) and organogenesis in the peripheral zone (PZ) is essential for the integrity, function, and maintenance of the SAM. Understanding how the ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.171462

    authors: Ung N,Lal S,Smith HM

    更新日期:2011-06-01 00:00:00

  • Characterization of P69E and P69F, two differentially regulated genes encoding new members of the subtilisin-like proteinase family from tomato plants.

    abstract::Subtilisin-like proteins represent an ancient family of serine proteases that are extremely widespread in living organisms. We report here the structure and genomic organization of two new transcriptionally active genes encoding proteins that belong to the P69 family of subtilisin-like proteases from tomato (Lycopersi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.122.1.67

    authors: Jordá L,Conejero V,Vera P

    更新日期:2000-01-01 00:00:00

  • Multiple forms of plant cytochromes p-450.

    abstract::Accumulating evidence indicates that there is a multiplicity of cytochrome P-450 enzymes in plants. These monooxygenases are implicated in the metabolism of sterols, terpenes, gibberellins, isoflavonoids, and xenobiotics. Evidence that cytochromes P-450 are involved in the detoxification of herbicides (chlorotoluron, ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.96.3.669

    authors: Donaldson RP,Luster DG

    更新日期:1991-07-01 00:00:00

  • Characteristics of tomato cell wall degradation in vitro: implications for the study of fruit-softening enzymes.

    abstract::The in vitro degradation of green tomato (Lycopersicon esculentum L.) cell walls by an extract of ripe fruits was characterized. The susceptibility of isolated walls to enzymolysis varied considerably among the different cultivars tested. Wall solubilization in vitro appeared to be nearly as extensive as that which ac...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.60.2.207

    authors: Wallner SJ,Bloom HL

    更新日期:1977-08-01 00:00:00

  • Effect of Low Root Medium pH on Net Proton Release, Root Respiration, and Root Growth of Corn (Zea mays L.) and Broad Bean (Vicia faba L.).

    abstract::The effect of low pH on net H(+) release and root growth of corn (Zea mays L.) and broad bean (Vicia faba L.) seedlings was investigated in short-term experiments at constant pH. Broad bean was more sensitive to low pH than corn: the critical values (pH values below which net H(+) release and root growth ceased) were ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.99.2.415

    authors: Yan F,Schubert S,Mengel K

    更新日期:1992-06-01 00:00:00

  • The role of salicylic acid in the induction of cell death in Arabidopsis acd11.

    abstract::Salicylic acid (SA) is implicated in the induction of programmed cell death (PCD) associated with pathogen defense responses because SA levels increase in response to PCD-inducing infections, and PCD development can be inhibited by expression of salicylate hydroxylase encoded by the bacterial nahG gene. The acd11 muta...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.059303

    authors: Brodersen P,Malinovsky FG,Hématy K,Newman MA,Mundy J

    更新日期:2005-06-01 00:00:00

  • Trehalose as cryoprotectant for the freeze preservation of carrot and tobacco cells.

    abstract::Suspension cultures of carrot (Daucus carota, line C1), tobacco (Nicotiana tabacum, line TX1), and Nicotiana plumbaginifolia (line NP) were frozen under controlled conditions with trehalose as the sole cryoprotectant. Maximal post-thaw viability (71-74%), measured by phenosafranin dye exclusion, was obtained with the ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.78.2.430

    authors: Bhandal IS,Hauptmann RM,Widholm JM

    更新日期:1985-06-01 00:00:00

  • Photosynthetic Potential and Accumulation of Assimilates in the Developing Chloroembryos of Cyamopsis tetragonoloba (L.) Taub.

    abstract::The photosynthetic potential of leaves and chloroembryos of Cyamopsis tetragonoloba (L.) Taub as measured by (14)C-bicarbonate fixation, Hill activity, and in vivo fluorescence transients is compared. On a chlorophyll basis, dark fixation of NaH(14)CO(3) in chloroembryos was 1.5 times higher than that of the leaf, whe...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.92.2.408

    authors: Kaladharan P,Vivekanandan M

    更新日期:1990-02-01 00:00:00

  • Suborganellar localization of proteinase catalyzing the limited hydrolysis of pumpkin globulin.

    abstract::Protein bodies were prepared from the cotyledons of pumpkin (Cucurbita sp.) seeds by employing a nonaqueous isolation method. Both light micrographic examination and the marker enzyme assays have shown that the isolated protein bodies were intact and contamination with other cell organelles or cytoplasmic components w...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.70.3.699

    authors: Hara-Nishimura I,Nishimura M,Matsubara H,Akazawa T

    更新日期:1982-09-01 00:00:00

  • Functional genomic analysis supports conservation of function among cellulose synthase-like a gene family members and suggests diverse roles of mannans in plants.

    abstract::Mannan polysaccharides are widespread among plants, where they serve as structural elements in cell walls, as carbohydrate reserves, and potentially perform other important functions. Previous work has demonstrated that members of the cellulose synthase-like A (CslA) family of glycosyltransferases from Arabidopsis (Ar...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.093989

    authors: Liepman AH,Nairn CJ,Willats WG,Sørensen I,Roberts AW,Keegstra K

    更新日期:2007-04-01 00:00:00

  • Protein Body Composition in Cuburbita maxima Cotyledons as Determined by Energy Dispersive X-Ray Analysis.

    abstract::Energy dispersive x-ray analysis was used to study the composition of certain protein body components in Cucurbita maxima cotyledons. The globoid crystal was rich in phosphorus, potassium, and magnesium. This elemental composition provides further evidence that the globoid crystal in squash cotyledon protein bodies is...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.55.5.913

    authors: Lott JN

    更新日期:1975-05-01 00:00:00

  • HEAT SHOCK FACTOR A8a Modulates Flavonoid Synthesis and Drought Tolerance.

    abstract::Drought is an important environmental factor affecting the growth and production of agricultural crops and fruits worldwide, including apple (Malus domestica). Heat shock factors (HSFs) have well-documented functions in stress responses, but their roles in flavonoid synthesis and the flavonoid-mediated drought respons...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.20.01106

    authors: Wang N,Liu W,Yu L,Guo Z,Chen Z,Jiang S,Xu H,Fang H,Wang Y,Zhang Z,Chen X

    更新日期:2020-11-01 00:00:00

  • Anomalous nonstoichiometry detected by dual label analysis of ribulose 1,5-bisphosphate carboxylase.

    abstract::When ribulose-1,5-bisphosphate carboxylase is assayed under N(2) using [(3)H]ribulose 1,5-bisphosphate and (14)CO(2), [(3)H]3-phosphoglycerate and [(14)C]3-phosphoglycerate are produced in nonstoichiometric amounts in a ratio which approaches 7 at low concentrations of CO(2) (2 micromolar) assuming a 1:1 ratio at V(ma...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.80.4.1055

    authors: Tomany MJ,Kent SS

    更新日期:1986-04-01 00:00:00

  • Soybean Seed Coat Peroxidase (A Comparison of High-Activity and Low-Activity Genotypes).

    abstract::Peroxidase activity in the seed coats of soybean (Glycine max [L.] Merr.) is controlled by the Ep locus. We compared peroxidase activity in cell-free extracts from seed coat, root, and leaf tissues of three EpEp cultivars (Harosoy 63, Harovinton, and Coles) to three epep cultivars (Steele, Marathon, and Raiden). Extra...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.4.1061

    authors: Gijzen M,Van Huystee R,Buzzell RI

    更新日期:1993-12-01 00:00:00

  • Mercury-induced Ethylene Formation and Abscission in Citrus and Coleus Explants.

    abstract::Mercury vapor induces ethylene formation and abscission in Citrus and Coleus explants. Both responses are markedly greater in the absence of CO(2). The stimulation of these metabolically complex processes indicates that the action of mercury vapor is not consistent with the more popular conception of mercury toxicity....

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.57.4.628

    authors: Goren R,Siegel SM

    更新日期:1976-04-01 00:00:00

  • Analysis of cell division and elongation underlying the developmental acceleration of root growth in Arabidopsis thaliana.

    abstract::To investigate the relation between cell division and expansion in the regulation of organ growth rate, we used Arabidopsis thaliana primary roots grown vertically at 20 degreesC with an elongation rate that increased steadily during the first 14 d after germination. We measured spatial profiles of longitudinal veloci...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.116.4.1515

    authors: Beemster GT,Baskin TI

    更新日期:1998-04-01 00:00:00

  • Signal peptide-dependent targeting of a rice alpha-amylase and cargo proteins to plastids and extracellular compartments of plant cells.

    abstract::alpha-Amylases are important enzymes for starch degradation in plants. However, it has been a long-running debate as to whether alpha-amylases are localized in plastids where starch is stored. To study the subcellular localization of alpha-amylases in plant cells, a rice (Oryza sativa) alpha-amylase, alphaAmy3, with o...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.042184

    authors: Chen MH,Huang LF,Li HM,Chen YR,Yu SM

    更新日期:2004-07-01 00:00:00

  • Three 1-aminocyclopropane-1-carboxylate synthase genes regulated by primary and secondary pollination signals in orchid flowers.

    abstract::The temporal and spatial expression patterns of three 1-aminocyclopropane-1-carboxylate (ACC) synthase genes were investigated in pollinated orchid (Phalaenopsis spp.) flowers. Pollination signals initiate a cascade of development events in multiple floral organs, including the induction of ethylene biosynthesis, whic...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.116.1.419

    authors: Bui AQ,O'Neill SD

    更新日期:1998-01-01 00:00:00

  • Phototropism and photoinhibition of basipolar transport of auxin in oat coleoptiles.

    abstract::We have proposed that the lateral inequality of auxin associated with the phototropic response is a consequence of a light-induced impairment of basipolar transport of the hormone. If this is so, the dose-response curve for photoinhibition of auxin transport should resemble that for phototropism. Further, the waveleng...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.44.4.491

    authors: Shen-Miller J,Cooper P,Gordon SA

    更新日期:1969-04-01 00:00:00

  • Light-induced growth promotion by SPA1 counteracts phytochrome-mediated growth inhibition during de-etiolation.

    abstract::Previous evidence has suggested that SPA1 is a signal transduction component that appears to require phytochrome A for function in seedling photomorphogenesis. Using digital image analysis, we examined the time course of growth inhibition induced by red light in spa1 mutants to test the interpretation that SPA1 functi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.126.3.1291

    authors: Parks BM,Hoecker U,Spalding EP

    更新日期:2001-07-01 00:00:00

  • In Vitro Fatty Acid Synthesis and Complex Lipid Metabolism in the Cyanobacterium, Anabaena Variabilis: II. Acyl Transfer and Complex Lipid Formation.

    abstract::In vitro fatty acid transfer to form complex lipids was observed in crude cell extracts of Anabaena variabilis using [1-(14)C]palmitoyl-acyl carrier protein, [1-(14)C]stearoyl-acyl carrier protein, and [1-(14)C]oleoyl-acyl carrier protein substrates. The data indicated that there was a rapid transfer of the fatty acid...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.75.3.700

    authors: Lem NW,Stumpf PK

    更新日期:1984-07-01 00:00:00

  • The Arabidopsis aleurone layer responds to nitric oxide, gibberellin, and abscisic acid and is sufficient and necessary for seed dormancy.

    abstract::Seed dormancy is a common phase of the plant life cycle, and several parts of the seed can contribute to dormancy. Whole seeds, seeds lacking the testa, embryos, and isolated aleurone layers of Arabidopsis (Arabidopsis thaliana) were used in experiments designed to identify components of the Arabidopsis seed that cont...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.093435

    authors: Bethke PC,Libourel IG,Aoyama N,Chung YY,Still DW,Jones RL

    更新日期:2007-03-01 00:00:00

  • Extinction coefficients of chlorophyll a and B in n,n-dimethylformamide and 80% acetone.

    abstract::We found inconsistencies in the commonly used data for chlorophyll analysis in 80% acetone. Recently developed extinction coefficients for chlorophyll b in N,N-dimethylformamide (DMF) based on values from 80% acetone are low as a result of these inconsistencies. We determined extinction coefficients of chlorophyll a (...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.77.2.483

    authors: Inskeep WP,Bloom PR

    更新日期:1985-02-01 00:00:00

  • Effects of low concentrations of o(3) on net photosynthesis, dark respiration, and chlorophyll contents in aging hybrid poplar leaves.

    abstract::Chronic exposure of hybrid poplar (Populus deltoides x trichocarpa) plants to low concentrations of ozone had negative impact upon net photosynthetic capacity, dark respiration, and leaf chlorophyll contents. Exposure to as much as 0.20 microliters per liter O(3) had no immediate effect on net photosynthesis (P(n)), b...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.73.2.291

    authors: Reich PB

    更新日期:1983-10-01 00:00:00

  • Enhancement of plant-microbe interactions using a rhizosphere metabolomics-driven approach and its application in the removal of polychlorinated biphenyls.

    abstract::Persistent organic pollutants, such as polychlorinated biphenyls (PCBs), are a global problem. We demonstrate enhanced depletion of PCBs using root-associated microbes, which can use plant secondary metabolites, such as phenylpropanoids. Using a "rhizosphere metabolomics" approach, we show that phenylpropanoids consti...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.102.016295

    authors: Narasimhan K,Basheer C,Bajic VB,Swarup S

    更新日期:2003-05-01 00:00:00

  • Induction of lipid metabolic enzymes during the endoplasmic reticulum stress response in plants.

    abstract::The endoplasmic reticulum (ER) stress response is a signal transduction pathway activated by the perturbation of normal ER metabolism. We used the maize (Zea mays) floury-2 (fl2) mutant and soybean (Glycine max) suspension cultures treated with tunicamycin (Tm) to investigate the ER stress response as it relates to ph...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.126.1.267

    authors: Shank KJ,Su P,Brglez I,Boss WF,Dewey RE,Boston RS

    更新日期:2001-05-01 00:00:00

  • Root Isoflavonoid Response to Grafting between Wild-Type and Nodulation-Mutant Soybean Plants.

    abstract::It was previously reported that the hypernodulating soybean (Glycine max [L.] Merr.) mutants, derived from the cultivar Williams, had higher root concentration of isoflavonoid compounds (daidzein, genistein, and coumestrol) than did Williams at 9 to 12 days after inoculation with Bradyrhizobium japonicum. These compou...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.96.4.1277

    authors: Cho MJ,Harper JE

    更新日期:1991-08-01 00:00:00

  • Bound auxin metabolism in cultured crown-gall tissues of tobacco.

    abstract::Bound auxin metabolism in cultured crown-gall tumor cells and pith callus of tobacco was examined by feeding radiolabeled auxins and auxin conjugates. In all tissues fed [(14)C]indoleacetic acid (IAA), at least one-third of the IAA was decarboxylated, and most of the remaining radiolabel occurred in a compound(s) whic...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.80.2.315

    authors: Vijayaraghavan SJ,Pengelly WL

    更新日期:1986-02-01 00:00:00

  • Characterization of Plasma Membrane-associated Adenosine Triphosphase Activity of Oat Roots.

    abstract::ATPase activity of plasma membranes isolated from oat (Avena sativa L. cv. Goodfield) roots was activated by divalent cations (Mg(2+) = Mn(2+) > Zn(2+) > Fe(2+) > Ca(2+)) and further stimulated by KCl and a variety of monovalent salts, both inorganic and organic. The enzyme exhibited greater specificity for cations th...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.52.1.6

    authors: Leonard RT,Hodges TK

    更新日期:1973-07-01 00:00:00

  • The purple cauliflower arises from activation of a MYB transcription factor.

    abstract::Anthocyanins are responsible for the color of many flowers, fruits, and vegetables. An interesting and unique Purple (Pr) gene mutation in cauliflower (Brassica oleracea var botrytis) confers an abnormal pattern of anthocyanin accumulation, giving the striking mutant phenotype of intense purple color in curds and a fe...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.164160

    authors: Chiu LW,Zhou X,Burke S,Wu X,Prior RL,Li L

    更新日期:2010-11-01 00:00:00