C(6)-[benzene ring]-indole-3-acetic Acid: a new internal standard for quantitative mass spectral analysis of indole-3-acetic Acid in plants.

Abstract:

:Indole-3-acetic acid (IAA) labeled with (13)C in the six carbons of the benzene ring is described for use as an internal standard for quantitative mass spectral analysis of IAA by gas chromatography/selected ion monitoring. [(13)C(6)]IAA was compared to the available deuterium labeled compounds and shown to offer the advantages of nonexchangeability of the isotope label, high isotopic enrichment, and chromatographic properties identical to that of the unlabeled compound. The utility of [(13)C(6)]IAA for measurement of endogenous IAA levels was demonstrated by analysis of IAA in Lemna gibba G-3.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Cohen JD,Baldi BG,Slovin JP

doi

10.1104/pp.80.1.14

subject

Has Abstract

pub_date

1986-01-01 00:00:00

pages

14-9

issue

1

eissn

0032-0889

issn

1532-2548

journal_volume

80

pub_type

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