Abstract:
:The auxin indole-3-acetic acid (IAA) has been shown to promote the biosynthesis of the active gibberellin (GA(1)) in shoots of pea (Pisum sativum). We used northern analysis to investigate the timing of IAA-induced changes in transcript levels of PsGA3ox1 (Mendel's LE), PsGA2ox1, PsGA2ox2, and PsGA20ox1, key genes for the later stages of GA(1) biosynthesis and metabolism in pea. Rapid (2-4 h) changes occurred in the transcript levels of PsGA3ox1, PsGA2ox1, and PsGA2ox2 after treatment with IAA. [(14)C]GA(1) metabolism studies in decapitated shoots indicated that IAA inhibits GA(1) deactivation, consistent with the suppression of PsGA2ox1 (SLN) transcript levels by IAA. Studies with the sln mutant also indicated that PsGA2ox1 activity is involved in GA(1) deactivation in decapitated shoots. Culture of excised internode tissue in the presence of auxin clearly demonstrated that internode tissue is a site of GA(1) biosynthesis per se. Excised internode tissue cultured in the presence/absence of cycloheximide showed that de novo protein synthesis is required for IAA-induced increases in PsGA3ox1 transcript levels. Auxin dose response studies indicated that IAA concentration is a critical determinant of GA(1) biosynthesis over 1 to 2 orders of magnitude, and a range of auxins was shown to affect the GA pathway.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
O'Neill DP,Ross JJdoi
10.1104/pp.010587subject
Has Abstractpub_date
2002-12-01 00:00:00pages
1974-82issue
4eissn
0032-0889issn
1532-2548journal_volume
130pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::Histone proteins play a central role in chromatin packaging, and modification of histones is associated with chromatin accessibility. SET domain [Su(var)3-9, Enhancer-of-zeste, Trithorax] proteins are one class of proteins that have been implicated in regulating gene expression through histone methylation. The relatio...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.102.013722
更新日期:2003-06-01 00:00:00
abstract::The soybean SAUR (Small Auxin-Up RNA) genes are transcriptionally induced by exogenous auxins within a few minutes after hormone application. This response is specifically induced by auxins primarily in epidermal and cortical cells within elongation zones of hypocotyls and epicotyls. We have previously shown that an 8...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.1.37
更新日期:1994-09-01 00:00:00
abstract::The effect of ozone was studied on the peroxidase activity from various compartments of Sedum album leaves (epidermis, intercellular fluid, residual cell material, and total cell material). The greatest increase following a 2-hour ozone exposure (0.4 microliters O(3) per liter) was observed in extracellular peroxidase...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.74.4.846
更新日期:1984-04-01 00:00:00
abstract::We have used cDNA microarray analysis to examine changes in gene expression during embryo development in soybean (Glycine max) and to compare gene expression profiles of two soybean cultivars that differ in seed isoflavonoid content. The analysis identified 5,910 genes that were differentially expressed in both soybea...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.086306
更新日期:2007-01-01 00:00:00
abstract::Leaflets of Vicia faba were pulse-labeled with (14)CO(2) to follow the subsequent movement of photosynthate between leaf tissues. Samples were taken during a (12)CO(2) chase, quick frozen, freeze-substituted, and embedded in methacrylate. Paradermal sections provided tissue samples consisting only of upper epidermis, ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.55.4.699
更新日期:1975-04-01 00:00:00
abstract::Drought stress affects cereals especially during the reproductive stage. The maize (Zea mays) drought transcriptome was studied using RNA-Seq analysis to compare drought-treated and well-watered fertilized ovary and basal leaf meristem tissue. More drought-responsive genes responded in the ovary compared with the leaf...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.200444
更新日期:2012-10-01 00:00:00
abstract::An NADPH-dependent NO2--reducing system was reconstituted in vitro using ferredoxin (Fd) NADP+ oxidoreductase (FNR), Fd, and nitrite reductase (NiR) from the green alga Chlamydomonas reinhardtii. NO2- reduction was dependent on all protein components and was operated under either aerobic or anaerobic conditions. NO2- ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.117.1.303
更新日期:1998-05-01 00:00:00
abstract::Increased levels of solar ultraviolet (290-320 nm) (UV-B) radiation could have profound effects on plant proteins because the aromatic amino acids in proteins absorb strongly in this spectral region. We have investigated the effects of UV-B radiation on plant proteins and have observed a novel 66-kD protein. This prod...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.1.221
更新日期:1995-09-01 00:00:00
abstract::Selenium-Binding Protein1 (SBP1) gene expression was studied in Arabidopsis (Arabidopsis thaliana) seedlings challenged with several stresses, including cadmium (Cd), selenium {selenate [Se(VI)] and selenite [Se(IV)]}, copper (Cu), zinc (Zn), and hydrogen peroxide (H(2)O(2)) using transgenic lines expressing the lucif...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.144808
更新日期:2009-10-01 00:00:00
abstract::From a study of the effects of Ca ions on the nodulation of subterranean clover in flowing culture solutions it is concluded that root infection or nodule initiation has a higher Ca requirement than either nodule development or host plant growth in the presence of fixed nitrogen.Increasing Ca concentration from 246 to...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.43.9.1362
更新日期:1968-09-01 00:00:00
abstract::The optimum temperature of protein synthesis in wheat seedlings (Triticum aestivum L.), measured as (14)C-leucine incorporation, depends on the growing temperature. Plants grown at reduced temperature (4 C) reach their optimum at 27.5 C, whereas plants kept at 36 C have the highest rate of protein synthesis at 35 C. T...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.56.5.590
更新日期:1975-11-01 00:00:00
abstract::We describe the development and utilization of a new imaging technology for plant biology, optical coherence microscopy (OCM), which allows true in vivo visualization of plants and plant cells. This novel technology allows the direct, in situ (e.g. plants in soil), three-dimensional visualization of cells and events i...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.123.1.3
更新日期:2000-05-01 00:00:00
abstract::The effects of CO2 enrichment on growth and N allocation of rice (Oryza sativa L.) were examined. The plants were grown hydroponically in growth chambers with a 14-h photoperiod (1000 [mu]mol quanta m-2 s-1) and a day/night temperature of 25/20[deg]C. From the 28th to 70th d after germination, the plants were exposed ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.115.1.199
更新日期:1997-09-01 00:00:00
abstract::O(2) consumption by the desiccation-tolerant moss Tortula ruralis and the desiccation-intolerant Cratoneuron filicinum increased markedly during the latter stages of desiccation. ATP content of the mosses during desiccation was not correlated with O(2) consumption, but was influenced by the rate at which the mosses lo...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.64.1.13
更新日期:1979-07-01 00:00:00
abstract::A novel mutant of Arabidopsis thaliana, arc6 (accumulation and replication of chloroplasts), has been isolated from a transfer DNA-mutagenized population of Arabidopsis seedlings. arc6 has the most extreme arc mutant phenotype we have yet described, with only one to three chloroplasts per leaf mesophyll cell compared ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.3.1169
更新日期:1994-11-01 00:00:00
abstract::Light induces beta-carotene synthesis in streptomycin-bleached Euglena gracilis Z. Light-adapted, chemostat-grown cells have up to 10-fold as much beta-carotene and 25% more protein than similarly grown, dark-adapted cells. Carotenogenesis does not occur under anaerobic conditions or in the presence of diphenylamine, ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.46.5.685
更新日期:1970-11-01 00:00:00
abstract::A radiation-induced mutant of Scotch spearmint (Mentha x gracilis) was shown to produce an essential oil containing principally C3-oxygenated p-menthane monoterpenes that are typical of peppermint, instead of the C6-oxygenated monoterpene family characteristic of spearmint. In vitro measurement of all of the enzymes r...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.96.3.744
更新日期:1991-07-01 00:00:00
abstract::Suberin is a complex hydrophobic polymer that acts as a barrier controlling water and solute fluxes and restricting pathogen infections. Suberin is deposited immediately outside of the plasmalemma in the cell wall of certain tissues such as endodermis of roots, aerial and underground periderms, and seed coats. Suberin...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.16.00834
更新日期:2016-07-01 00:00:00
abstract::Lysine acetylation (LysAc), a form of reversible protein posttranslational modification previously known only for histone regulation in plants, is shown to be widespread in Arabidopsis (Arabidopsis thaliana). Sixty-four Lys modification sites were identified on 57 proteins, which operate in a wide variety of pathways/...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.110.165852
更新日期:2011-04-01 00:00:00
abstract::Establishment of symbiosis between legumes and rhizobia requires bacterial Nod factors (NFs). The concentration of these lipochitooligosaccharides in the rhizosphere is influenced by plant enzymes. NFs induce on pea (Pisum sativum) a particular extracellular NF hydrolase that releases lipodisaccharides from NFs from S...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.061705
更新日期:2005-10-01 00:00:00
abstract::Ethylene receptors are multispanning membrane proteins that negatively regulate ethylene responses via the formation of a signaling complex with downstream elements. To better understand their biochemical functions, we investigated the membrane topology and subcellular localization of CmERS1, a melon (Cucumis melo) et...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.080523
更新日期:2006-06-01 00:00:00
abstract::Cyclin-dependent kinases (CDKs) form a conserved superfamily of eukaryotic serine-threonine protein kinases whose activity requires the binding of a cyclin protein. CDKs are involved in many aspects of cell biology and notably in the regulation of the cell cycle. Three cDNAs encoding a C-type CDK, and a member of each...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.126.4.1403
更新日期:2001-08-01 00:00:00
abstract::The red alga Gelidium coulteri Harv. photosynthetically fixed [(14)C] bicarbonate at high rates under defined conditions in unialgal laboratory culture. The fixation rate and flow of photosynthate into various end products were dependent on the nitrogen status of the tissue. Plants fed luxury levels of nitrogen (appro...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.82.1.136
更新日期:1986-09-01 00:00:00
abstract::Phosphatidylcholine (PC) is a major membrane phospholipid and a precursor for major signaling molecules. Understanding its synthesis is important for improving plant growth, nutritional value, and resistance to stress. PC synthesis is complex, involving several interconnected pathways, one of which proceeds from serin...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.18.00457
更新日期:2018-08-01 00:00:00
abstract::We have shown that amide-linked IAA (indole-3-acetic acid) conjugates accumulated to high levels during maturation of bean seeds (K. Bialek and J.D. Cohen [1989] Plant Physiol 91: 775-779). In the present study, we were interested in the fate of these and other IAA conjugates during seed germination. The content of am...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.100.4.2002
更新日期:1992-12-01 00:00:00
abstract::Lotus pedunculatus L., Medicago sativa L., Macroptilium atropurpureum, Glycine max, and Trifolium repens L. were grown in a N-free medium and inoculated with one of ten Rhizobium strains. Dry matter, N content, and delta(15)N values were determined for various plant parts.Nodules, with the exception of those from luce...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.72.4.1001
更新日期:1983-08-01 00:00:00
abstract::The polyunsaturated fatty acid content is one of the major factors influencing the quality of vegetable oils. Edible oils rich in monounsaturated fatty acid provide improved oil stability, flavor, and nutrition for human and animal consumption. In plants, the microsomal omega-6 desaturase-catalyzed pathway is the prim...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.110.1.311
更新日期:1996-01-01 00:00:00
abstract::Fully hydrated lettuce (Lactuca sativa L.) seeds showed dual freezing exotherms (-9 and -18 degrees C), even after 10 hours imbibition. Only the -9 degrees C exotherm was observed in seeds imbibed for 20 hours, but without external nucleation, all water in the embryo supercooled. Results indicate that the endosperm ac...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.70.5.1571
更新日期:1982-11-01 00:00:00
abstract::Plants are able to integrate exogenous 1-deoxy-D-xylulose (DX) into the 2C-methyl-D-erythritol 4-phosphate pathway, implicated in the biosynthesis of plastidial isoprenoids. Thus, the carbohydrate needs to be phosphorylated into 1-deoxy-D-xylulose 5-phosphate and translocated into plastids, or vice versa. An enzyme ca...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.086652
更新日期:2006-10-01 00:00:00
abstract::Plant development requires regulation of both cell division and differentiation. The class 1 KNOTTED1-like homeobox (KNOX) genes such as knotted1 (kn1) in maize (Zea mays) and SHOOTMERISTEMLESS in Arabidopsis (Arabidopsis thaliana) play a role in maintaining shoot apical meristem indeterminacy, and their misexpression...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.076075
更新日期:2006-08-01 00:00:00