Abstract:
:Membrane preparation (sedimenting between 13,000(g) and 80,000(g)) of germinating radish seeds (Raphanus sativus L.) was active in hydrolyzing ATP and, to a lesser extent, a variety of other phosphorylated compounds. Dicyclohexylcarbodiimide (DCCD) and diethylstilbestrol significantly inhibited the ATPase activity (40%) while their effect on hydrolysis of other phosphorylated compounds was much less.The sucrose density gradient analysis of the membrane preparation showed that the position of the DCCD-sensitive K(+)-dependent ATPase was similar to that found for plasma membrane of other plant material.Cholate treatment of membrane preparation removes almost all phospholipids, and ATPase activity is barely detectable. However, the addition of polar lipids completely restores the ATPase activity but does not restore general phosphatase activity.The ATPase of the polar lipids restored cholate preparation, showed a high sensitivity to DCCD and diethylstilbestrol (up to 90% inhibition), a complete dependence on Mg(2+), and a strong dependence on K(+) at low concentration; the pH optimum of ATPase was close to 6.5, and the K(m) for ATP-Mg was 0.51 millimolar. ATPase activity was much greater when polar lipids from 24-hour-germinated seeds were added.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Cocucci M,Ballarin-Denti Adoi
10.1104/pp.68.2.377subject
Has Abstractpub_date
1981-08-01 00:00:00pages
377-81issue
2eissn
0032-0889issn
1532-2548journal_volume
68pub_type
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