Abstract:
:The presence of chloroplasts in guard cells from leaf epidermis, coleoptile, flowers, and albino portions of variegated leaves was established by incident fluorescence microscopy, thus confirming the notion that guard cell chloroplasts are remarkably conserved. Room temperature emission spectra from a few chloroplasts in a single guard cell of Vicia faba showed one major peak at around 683 nanometers. Low-temperature (77 K) emission spectra from peels of albino portions of Chlorophytum comosum leaves and from mesophyll chloroplasts of green parts of the same leaves showed major peaks at around 687 and 733 nanometers, peaks usually attributed to photosystem II and photosystem I pigment systems, respectively. Spectra of peels of V. faba leaves showed similar peaks. However, fluorescence microscopy revealed that the Vicia peels, as well as those from Allium cepa and Tulipa sp., were contaminated with non-guard cell chloroplasts which were practically undetectable under bright field illumination. These observations pose restrictions on the use of epidermal peels as a source of isolated guard cell chloroplasts. Studies on the 3-(3,4-dichlorophenyl)-1,1-dimethylurea-sensitive variable fluorescence kinetics of uncontaminated epidermal peels of C. comosum indicated that guard cell chloroplasts operate a normal, photosystem II-dependent, linear electron transport. The above properties in combination with their reported inability to fix CO(2) photosynthetically may render the guard cell chloroplasts optimally suited to supply the reducing and high-energy phosphate equivalents needed to sustain active ion transport during stomatal opening in daylight.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Zeiger E,Armond P,Melis Adoi
10.1104/pp.67.1.17subject
Has Abstractpub_date
1981-01-01 00:00:00pages
17-20issue
1eissn
0032-0889issn
1532-2548journal_volume
67pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::ADP-glucose pyrophosphorylase was partially purified from Chlorella vulgaris 11h. 3-Phosphoglycerate activated the enzyme by lowering the Michaelis constant for glucose-1-phosphate (from 0.97 to 0.36 millimolar in the presence of 2 millimolar phosphoglycerate) and ATP (from 0.23 to 0.10 millimolar), as well as increas...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.78.3.601
更新日期:1985-07-01 00:00:00
abstract::The development of photochemical activity and carbon assimilation in light-grown cucumber (Cucumis sativus L. cv Natsusairaku) leaves was studied to determine the pattern of acquisition and its relationship to leaf growth and expansion. Measurements of chlorophyll a fluorescence showed that leaves acquire photochemica...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.93.3.1078
更新日期:1990-07-01 00:00:00
abstract::Nuclear pore complexes (NPCs) mediate the transport of RNA and other cargo between the nucleus and the cytoplasm. In vertebrates, the NPC protein TRANSLOCATED PROMOTER REGION (TPR) is associated with the inner filaments of the nuclear basket and is thought to serve as a scaffold for the assembly of transport machinery...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.107.100735
更新日期:2007-07-01 00:00:00
abstract::Pattern-recognition receptors (PRRs), which consist of receptor kinases (RKs) and receptor-like proteins, sense microbe- and host-derived molecular patterns associated with pathogen infection to trigger immune responses in plants. Several kinases of the 46-member Arabidopsis (Arabidopsis thaliana) receptor-like cytopl...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.18.00486
更新日期:2018-08-01 00:00:00
abstract::Nearly all polysaccharides in plant cell walls are O-acetylated, including the various pectic polysaccharides and the hemicelluloses xylan, mannan, and xyloglucan. However, the enzymes involved in the polysaccharide acetylation have not been identified. While the role of polysaccharide acetylation in vivo is unclear, ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.110.168989
更新日期:2011-03-01 00:00:00
abstract::Levels of gibberillins (GAs) and of abscisic acid (ABA) in attached leaves of romaine lettuce (Lactuca sativa L.) declined as the leaf became older. The time course of changes in hormone levels, determined in detached lettuce leaves kept in darkness, revealed that a sharp decline in GAs accompanied by a moderate rise ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.62.2.224
更新日期:1978-08-01 00:00:00
abstract::Membrane fractions from mature silver beet (Beta vulgaris) deveined leaf and leaf stem homogenates have associated Ca(2+) -dependent protein kinase. The Ca(2+) -dependent protein kinase activity is associated with plasma membranes (density 1.14-1.18 grams per cubic centimeter) as determined from copurification on isop...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.88.1.164
更新日期:1988-09-01 00:00:00
abstract::Transgenic tobacco plants carrying a fusion between the nopaline synthase (nos) promoter and chloramphenicol acetyltransferase (CAT) reporter gene (cat) were studied for their inducibility by salicylic acid (SA) or methyl jasmonate (MJ) treatments. Either chemical significantly increased CAT activity to a level much h...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.103.1.97
更新日期:1993-09-01 00:00:00
abstract::The influence of light or darkness on stomatal opening in epidermal strips of Commelina benghalensis was evaluated in the presence or absence of O(2) and/or metabolic inhibitors. Opening was restricted in nitrogen and was promoted by NADH and acids of the tricarboxylic acid cycle (succinate and alpha-ketoglutarate) in...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.67.2.385
更新日期:1981-02-01 00:00:00
abstract::The sink-source conversion in developing leaves of tobacco (Nicotiana tabacum L.) was studied to determine whether import termination is caused by the onset of export or is related to achievement of positive carbon balance. Albino shoots were grown in vitro and grafted to detopped stems of green tobacco plants. Termin...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.76.1.45
更新日期:1984-09-01 00:00:00
abstract::An isotope-dilution method has been developed for the assay of free indole-3-acetic acid and ester indole-3-acetic acid as measured by indole-3-acetic acid liberated by mild alkaline hydrolysis. Application of this method to seedlings of Avena sativa and Zea mays indicates the upper limit of free indole-3-acetic acid ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.54.3.257
更新日期:1974-09-01 00:00:00
abstract::The effect of ultraviolet B (UV-B) radiation on the thermal sensitivity of cucumber (Cucumis sativus L.) was studied using UV-B-sensitive cv Poinsett 76 and UV-B-resistant cv Ashley grown under control and elevated (300 mW m-2) UV-B radiation levels. Using both cotyledon and leaf discs, the ability of the tissue to re...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.2.395
更新日期:1994-02-01 00:00:00
abstract::The expression of CHALCONE SYNTHASE (CHS) expression is an important control step in the biosynthesis of flavonoids, which are major photoprotectants in plants. CHS transcription is regulated by endogenous programs and in response to environmental signals. Luciferase reporter gene fusions showed that the CHS promoter ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.005405
更新日期:2002-09-01 00:00:00
abstract::Fruit ripening and abscission are associated with an ethylene burst in several melon (Cucumis melo) genotypes. In cantaloupe as in other climacteric fruit, exogenous ethylene can prematurely induce abscission, ethylene production, and ripening. Melon genotypes without fruit abscission or without ethylene burst also ex...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.010613
更新日期:2002-05-01 00:00:00
abstract::In maize (Zea mays L., cv Contessa), nitrogen (NO(3) (-)) limitation resulted in a reduction in shoot growth and photosynthetic capacity and in an increase in the leaf zeaxanthin contents. Nitrogen deficiency had only a small effect on the quantum yield of CO(2) assimilation but a large effect on the light-saturated r...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.94.3.1436
更新日期:1990-11-01 00:00:00
abstract::Effects of water stress on ethylene evolution from excised leaf segments and intact plants of wheat (Triticum aestivum L. cv Katepwa) were studied. Excised leaf segments of 8 day or 6 week old plants were dried until they lost 8% of their fresh weight (water potential about -2.3 megapascals). These and nondried contro...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.96.2.406
更新日期:1991-06-01 00:00:00
abstract::Seed dormancy is a common phase of the plant life cycle, and several parts of the seed can contribute to dormancy. Whole seeds, seeds lacking the testa, embryos, and isolated aleurone layers of Arabidopsis (Arabidopsis thaliana) were used in experiments designed to identify components of the Arabidopsis seed that cont...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.093435
更新日期:2007-03-01 00:00:00
abstract::Excised corn roots, Zea mays, had radioactively labeled solution applied at two points along their length, for 1 minute, and were then kept in dilute, unlabeled nutrient solution. During this "chase" period, exudate was collected at 1-minute intervals, and its content of radioions was determined. Two pulses of label a...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.52.4.346
更新日期:1973-10-01 00:00:00
abstract::Stilbenes are considered the most important phytoalexin group in grapevine (Vitis vinifera) and they are known to contribute to the protection against various pathogens. The main stilbenes in grapevine are resveratrol and its derivatives and, among these, pterostilbene has recently attracted much attention due both to...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.126003
更新日期:2008-11-01 00:00:00
abstract::Circadian clocks drive rhythms with a period near 24 h, but the molecular basis of the regulation of the period of the circadian clockis poorly understood. We previously demonstrated that metabolites affect the free-running period of the circadian oscillator of Arabidopsis (Arabidopsis thaliana), with endogenous sugar...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.18.00571
更新日期:2018-09-01 00:00:00
abstract::Saline-soluble glycinins and insoluble glutelins are the major storage proteins in soybean (Glycine max) and rice (Oryza sativa), respectively. In spite of their differences in solubility properties, both proteins are members of the 11S globulin gene family based on their similarities in primary sequences and processi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.120.4.1063
更新日期:1999-08-01 00:00:00
abstract::The carotenoid content of thylakoids and envelopes isolated from dark-or light-treated spinach (Spinacia oleracea L.) chloroplasts was compared. In thylakoids, light induced a decrease of violaxanthin parallel with a stoichiometric increase of zeaxanthin due to violaxanthin deepoxidation. In envelopes, violaxanthin wa...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.61.4.530
更新日期:1978-04-01 00:00:00
abstract::When intact pea (Pisum sativum L.) plants are illuminated, the glycolytic enzyme phosphofructokinase is inactivated. In crude extracts the enzyme is inhibited by dithiothreitol. It would seem that this cytoplasmic enzyme, like glucose-6-P dehydrogenase, is light-inactivated when the enzymes of photosynthetic carbon me...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.55.2.199
更新日期:1975-02-01 00:00:00
abstract::Red pines (Pinus resinosa Ait.) were grown in a pasteurized sandy loam either unamended with phosphate or fertilized with one of two levels of phosphate (34 or 136 mg/kg) as superphosphate, and with and without addition of Hebeloma arenosa inoculum. Shoot and total dry weights of mycorrhizal seedlings grown in soil un...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.100.2.713
更新日期:1992-10-01 00:00:00
abstract::A synthetic green fluorescent protein (GFP) gene (pgfp) was constructed to improve GFP expression in plants. Corn and tobacco protoplast transient assays showed that pgfp gave about 20-fold brighter fluorescence than the wild-type gene (gfp). Replacement of the serine at position 65 with a threonine (S65Tpgfp) or a cy...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.3.893
更新日期:1996-11-01 00:00:00
abstract::Auxins and cytokinins support cell division in tissue and cell cultures. In cytokinin-independent pear (Pyrus communis) cells, omission of 2,4-dichlorophenoxyacetic acid (2,4-D) from the medium for two successive transfers leads to rapid cell lysis, unless the osmolarity is raised to 0.4 molar with mannitol. Use of th...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.64.5.814
更新日期:1979-11-01 00:00:00
abstract::We compared nodule initiation in lateral roots of Pisum sativum (L.) cv Sparkle and in a low-nodulating mutant E2 (sym 5). In Sparkle, about 25% of the infections terminated in the epidermis, a similar number stopped in the cortex, and 50% resulted in the formation of a nodule meristem or an emerged nodule. The mutant...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.97.3.1206
更新日期:1991-11-01 00:00:00
abstract::Amyloplast envelope membranes isolated from cultured, white-wild cells of sycamore (Acer pseudoplatanus L.) have been found to contain a Mg(2+)-ATPase, ranging in specific activity from 5 to 30 nanomoles per minute per milligram protein. This ATPase hydrolyzes a broad range of nucleoside triphosphates, whereas it hydr...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.88.1.119
更新日期:1988-09-01 00:00:00
abstract::Lettuce (Lactuca sativa) seeds var. Grand Rapids could be maintained many weeks in the dark without germination. Following illumination with white light, a gradual increase in polyribosome population up to the time of germination was demonstrated by sucrose gradient centrifugation. Polysomes could not be detected in i...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.50.6.671
更新日期:1972-12-01 00:00:00
abstract::Sex determination in cucumber (Cucumis sativus L.) is controlled largely by three genes: F, m, and a. The F and m loci interact to produce monoecious (M_f_) or gynoecious (M_f_) sex phenotypes. Ethylene and factors that induce ethylene biosynthesis, such as 1-aminocyclopropane-1-carboxylate (ACC) and auxin, also enhan...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.3.987
更新日期:1997-03-01 00:00:00