Abstract:
:The expression patterns of five genes (PsGA20ox1, PsGA20ox2, PsGA3ox1, PsGA2ox1, and PsGA2ox2) encoding five regulatory gibberellin (GA) biosynthesis enzymes (two GA 20-oxidases, a GA 3beta-hydroxylase, and two GA 2beta-hydroxylases) were examined to gain insight into how these genes coordinate GA biosynthesis during germination and early postgermination stages of the large-seeded dicotyledonous plant pea (Pisum sativum). At the time the developing embryo fills the seed coat, high mRNA levels of PsGA20ox2 (primarily responsible for conversion of C20-GAs to GA(20)), PsGA2ox1 (primarily responsible for conversion of GA(20) to GA(29)), and PsGA2ox2 (primarily responsible for conversion of GA(1) to GA(8)) were detected in the seeds, along with high GA(20) and GA(29) levels, the enzymatic products of these genes. Embryo maturation was accompanied by a large reduction in PsGA20ox2 and PsGA2ox1 mRNA and lower GA(20) and GA(29) levels. However, PsGA2ox2 transcripts remained high. Following seed imbibition, GA(20) levels in the cotyledons decreased, while PsGA3ox1 mRNA and GA(1) levels increased, implying that GA(20) was being used for de novo synthesis of GA(1). The presence of the embryo axis was required for stimulation of cotyledonary GA(1) synthesis at the mRNA and enzyme activity levels. As the embryo axis doubled in size, PsGA20ox1 and PsGA3ox1 transcripts increased, both GA(1) and GA(8) were detectable, PsGA2ox2 transcripts decreased, and PsGA2ox1 transcripts remained low. Cotyledonary-, root-, and shoot-specific expression of these GA biosynthesis genes and the resultant endogenous GA profiles support a key role for de novo GA biosynthesis in each organ during germination and early seedling growth of pea.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Ayele BT,Ozga JA,Kurepin LV,Reinecke DMdoi
10.1104/pp.106.086199subject
Has Abstractpub_date
2006-11-01 00:00:00pages
1267-81issue
3eissn
0032-0889issn
1532-2548pii
pp.106.086199journal_volume
142pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::It is now generally accepted that a phosphoinositide cycle is involved in the transduction of a variety of signals in plant cells. In animal cells, the binding of D-myo-inositol 1,4,5-trisphosphate (InsP(3)) to a receptor located on the endoplasmic reticulum (ER) triggers an efflux of calcium release from the ER. Site...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.124.1.475
更新日期:2000-09-01 00:00:00
abstract::Proplastids and prolamellar bodies with tubular membranes were isolated from the dark grown primary leaves of bean seedlings (Phaseolus vulgaris L.). The combination of fluorescence microscopy and negative contrast electron microscopy provided the tentative identification of protochlorophyll holochrome as a constituen...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.43.11.1769
更新日期:1968-11-01 00:00:00
abstract::Surface glandular trichomes distributed throughout the aerial parts of sweet basil (Ocimum basilicum) produce and store monoterpene, sesquiterpene, and phenylpropene volatiles. Three distinct basil chemotypes were used to examine the molecular mechanisms underlying the divergence in their monoterpene and sesquiterpene...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.051318
更新日期:2004-11-01 00:00:00
abstract::Changes in diisopropylfluorophosphate (DFP)-binding proteins during development and senescence of spinach (Spinacia oleracea) leaves were followed using [(3)H]DFP and sodium dodecylsulfate-polyacrylamide gel electrophoresis-fluorography. Experiments using a series of aging stages of leaves attached to plants and ones ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.90.1.338
更新日期:1989-05-01 00:00:00
abstract::An organ-specific invertase deficiency affecting only the primary root system is described in the Oh 43 inbred maize (Zea mays). Invertases (acid and neutral/soluble and insoluble) were assayed in various tissues of hybrid (NK 508) and inbred (Oh 43, W 22) maize lines to determine the basis for an early report that Oh...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.97.2.523
更新日期:1991-10-01 00:00:00
abstract::The relationship among physiological, injury, growth, and yield responses was examined in field-grown green pepper (Capsicum annuum L. ;California Wonder') subjected to two airborne environmental stresses. The primary objectives were to determine if the stresses could cause alterations in the plant responses, and to d...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.88.2.477
更新日期:1988-10-01 00:00:00
abstract::Chromatin isolated from soybean (Glycine max L., var. Wayne) hypocotyls was capable of catalyzing the polymerization of labeled deoxyribonucleoside triphosphate in the presence of the three other deoxyribonucleoside triphosphates into a trichloroacetic acid-insoluble product. This product was insensitive to base hydro...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.48.1.43
更新日期:1971-07-01 00:00:00
abstract::Transgenic tobacco plants carrying a fusion between the nopaline synthase (nos) promoter and chloramphenicol acetyltransferase (CAT) reporter gene (cat) were studied for their inducibility by salicylic acid (SA) or methyl jasmonate (MJ) treatments. Either chemical significantly increased CAT activity to a level much h...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.103.1.97
更新日期:1993-09-01 00:00:00
abstract::We compared the subcellular distribution of native and artificial reticuloplasmins in endosperm, callus, and leaf tissues of transgenic rice (Oryza sativa) to determine the distribution of these proteins among endoplasmic reticulum (ER) and post-ER compartments. The native reticuloplasmin was calreticulin. The artific...
journal_title:Plant physiology
pub_type: 杂志文章
doi:
更新日期:2001-11-01 00:00:00
abstract::Germination potential in nondormant, upper cocklebur (Xanthium pensylvanicum Wallr.) seeds, which were incapable of germinating under constant temperatures below 25 C in air, was increased by exposure to diurnally alternating temperatures. The cocklebur seeds failed to respond to the temperature fluctuations in the be...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.61.3.437
更新日期:1978-03-01 00:00:00
abstract::The cytokinin activity has been isolated and identified from extracts of immature mango (Mangifera indica L.) seeds. The structures of zeatin, zeatin riboside, and N(6)-(Delta(2)-isopentenyl)adenine riboside were confirmed on the basis of their chromatographic behavior and mass spectra of trimethylsilyl derivatives. B...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.71.2.356
更新日期:1983-02-01 00:00:00
abstract::The action spectrum of apparent photosynthesis for attached radish (Raphanus sativus L. var. Early Scarlet Globe) and corn (Zea mays L. var. Pride V.) leaves was measured at 300 mul/l CO(2) and both 21% and 2% O(2). The spectra were measured at light intensities where apparent photosynthesis was proportional to intens...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.44.5.678
更新日期:1969-05-01 00:00:00
abstract::Nitrogen is an essential macronutrient required for plant growth and development. A number of genes respond to nitrogen starvation conditions. However, the functions of most of these nitrogen starvation-responsive genes are unclear. Our recent survey suggested that many microRNAs (miRNAs) are responsive to nitrogen st...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.228635
更新日期:2014-02-01 00:00:00
abstract::A new hydroxycinnamoyl-CoA:putrescine hydroxycinnamoyltransferase (PHT) was detected in two variant lines of Nicotiana tabacum L. (TX1, TX4) accumulating markedly different levels of caffeoylputrescine. The enzyme accepted only the aliphatic diamines putrescine, cadaverine and 1,3-diaminopropane at a ratio of 100:33:8...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.89.2.488
更新日期:1989-02-01 00:00:00
abstract::Fluxes of mineral nutrients in the xylem are strongly influenced by interactions with the surrounding stem tissues and are probably regulated by them. Toward a mechanistic understanding of these interactions, we applied stable isotope tracers of magnesium, potassium, and calcium continuously to the transpiration strea...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.143776
更新日期:2010-02-01 00:00:00
abstract::The O(2) concentration in intact and osmotically disrupted isolated spinach (Spinacia oleracea, L.) chloroplasts during photosynthesis was estimated. The chloroplasts were allowed to reduce 3-phosphoglycerate, CO(2), or ferricyanide in light until the rate of O(2) production was linear. When the light was turned off O...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.60.6.903
更新日期:1977-12-01 00:00:00
abstract::To function, the catalytic sites of Rubisco (EC 4.1.1.39) need to be activated by the reversible carbamylation of a lysine residue within the sites followed by rapid binding of magnesium. The activation of Rubisco in vivo requires the presence of the regulatory protein Rubisco activase. This enzyme is thought to aid t...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.056077
更新日期:2005-02-01 00:00:00
abstract::A novel alliinase (EC 4.4.1.4) was detected and purified from the roots of the Amazonian medicinal plant Petiveria alliacea. The isolated enzyme is a heteropentameric glycoprotein composed of two alpha-subunits (68.1 kD each), one beta-subunit (56.0 kD), one gamma-subunit (24.8 kD), and one delta-subunit (13.9 kD). Th...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.142430
更新日期:2009-11-01 00:00:00
abstract::The contribution of the alternative pathway in root respiration of Pisum sativum L. cv Rondo, Plantago lanceolata L., and Plantago major L. ssp major was determined by titration with salicylhydroxamate (SHAM) in the absence and presence of cyanide. SHAM completely inhibited the cyanide-resistant component of root resp...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.75.3.813
更新日期:1984-07-01 00:00:00
abstract::Cytochrome P450s (P450s) constitute one of the major classes of enzymes that are responsible for detoxification of exogenous molecules both in animals and plants. On the basis of its inducibility by exogenous chemicals, we recently isolated a new plant P450, CYP76B1, from Jerusalem artichoke (Helianthus tuberosus) and...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.118.3.1049
更新日期:1998-11-01 00:00:00
abstract::The effect of temperature on Hill activity has been compared in chilling-sensitive and chilling-resistant plants. The Arrhenius activation energy (Ea) for the photoreduction of 2,6-dichlorophenolindophenol by chloroplasts isolated from two chilling-sensitive plants, mung bean (Vigna radiata L. var. Mungo) and maize (Z...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.59.6.1141
更新日期:1977-06-01 00:00:00
abstract::The light and CO(2) response of (a) photosynthesis, (b) the activation state and total catalytic efficiency (k(cat)) of ribulose-1,5-bisphosphate carboxylase (rubisco), and (c) the pool sizes of ribulose 1,5-bisphosphate, (RuBP), ATP, and ADP were studied in the C(3) annuals Chenopodium album and Phaseolus vulgaris at...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.94.4.1735
更新日期:1990-12-01 00:00:00
abstract::Changes in the physical state of microsomal membrane lipids during senescence of rose flower petals (Rosa hyb. L. cv Mercedes) were measured by x-ray diffraction analysis. During senescence of cut flowers held at 22 degrees C, lipid in the ordered, gel phase appeared in the otherwise disordered, liquid-crystalline pha...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.83.4.1037
更新日期:1987-04-01 00:00:00
abstract::Chromosomal virulence (chv) mutants of Agrobacterium tumefaciens have been reported to be deficient in binding to cells of zinnia, tobacco, and bamboo. The mutants are nonpathogenic on stems of Kalanchoë, sunflower, tomato, Jerusalem artichoke, and tobacco, but they cause tumors on tubers of Solanum tuberosum. We used...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.91.1.113
更新日期:1989-09-01 00:00:00
abstract::We used Phytotyping4D to investigate the contribution of clock and light signaling to the diurnal regulation of rosette expansion growth and leaf movement in Arabidopsis (Arabidopsis thaliana). Wild-type plants and clock mutants with a short (lhycca1) and long (prr7prr9) period were analyzed in a T24 cycle and in T-cy...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.17.00503
更新日期:2017-07-01 00:00:00
abstract::To monitor the expression of T-DNA-tagged plant genes in vivo, a collection of 20,261 transgenic lines of Arabidopsis (Columbia-0) were generated with the promoter trap vector pTluc, which carries a promoterless firefly luc (luciferase) reporter gene linked to the right T-DNA border. By detection of bioluminescence in...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.103.027151
更新日期:2004-01-01 00:00:00
abstract::Understanding the relationship of the size and shape of an organism to the size, shape, and number of its constituent cells is a basic problem in biology; however, numerous studies indicate that the relationship is complex and often nonintuitive. To investigate this problem, we used a system for the inducible expressi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.111.176073
更新日期:2011-08-01 00:00:00
abstract::An automatic digital diffusion porometer circuit is described. The circuit is highly stable with respect to temperature and supply voltage. The device is capable of high timing accuracy over very short measurement intervals, so that measurements may be made rapidly without the danger of stomatal changes occurring duri...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.53.1.47
更新日期:1974-01-01 00:00:00
abstract::A 2-step, 1-dimensional thin-layer chromatographic procedure for isolating indoleacetic acid (IAA) was developed and utilized in investigations of the biosynthesis of IAA from tryptophan-(14)C in cell-free extracts of pea (Pisum sativum L.) shoot tips. Identification of a (14)C-product as IAA was by (a) co-chromatogra...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.42.12.1787
更新日期:1967-12-01 00:00:00
abstract::The ictA gene, renamed ndhL in this paper, essential to inorganic carbon transport of Synechocystis PCC6803, was expressed in Eschericia coli as a fusion protein with glutathione S-transferase. An antibody was raised against this fusion protein. Western analysis of the thylakoid membrane of wild-type (WT) Synechocysti...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.99.4.1604
更新日期:1992-08-01 00:00:00