Abstract：
:The specific activity of phosphoenolpyruvate (PEP) measured at a saturating level of substrate diminishes as the enzyme is diluted at about the same rate that specific light scattering by the diluted enzyme decreases. The presence of PEP in the assay causes an increase in activity with increasing dilution. This is accompanied by an increase in light scattering of the diluted enzyme. The reverse situation obtains with the addition of malate to assays: the activity decreases with increasing dilution but light scattering is not substantially changed, indicating that the enzyme is already brought to a smaller aggregate by the dilution itself. In this case, the inhibition by malate in the assay probably is the noncompetitive type not involved in regulatory control by malate. Glucose-6-phosphate in the range from 1 to 6 millimolar causes an increase in activity of the enzyme run at a substrate level less than K(m), and an associated increase in light scattering is found, indicating an increase in the mean size of the enzyme. When PEP is added to a 1/80 diluted enzyme, light scattering increases and is associated with a more rapid activity of the enzyme. When malate is added to the same cuvette, the activity decreases and the light scattering diminishes, thus showing that the ligand response is immediately reversible. When malate is added first, followed by PEP, the reverse sequence of activity and light scattering change is observed.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Willeford KO,Wedding RTdoi
10.1104/pp.99.2.755subject
Has Abstractpub_date
1992-06-01 00:00:00pages
755-8issue
2eissn
0032-0889issn
1532-2548journal_volume
99pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract：:The problem of determining gas exchange rates from flow system data under nonsteady state conditions is analyzed. A correction factor is presented for obtaining constant rates under nonsteady state conditions. A general formula for obtaining any rate under nonsteady state conditions is also given. Turnover time is def...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.56.5.680
更新日期：1975-11-01 00:00:00
abstract：:Subtilisin-like proteins represent an ancient family of serine proteases that are extremely widespread in living organisms. We report here the structure and genomic organization of two new transcriptionally active genes encoding proteins that belong to the P69 family of subtilisin-like proteases from tomato (Lycopersi...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.122.1.67
更新日期：2000-01-01 00:00:00
abstract：:Theoretical analysis of the CO(2) assimilation and water loss by single leaves suggests that the water use efficiency of C(4) species decreases as stomatal resistance increases. To confirm this hypothesis for a complete maize crop, results from computer simulations and a field experiment were compiled for varying stom...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.56.2.245
更新日期：1975-08-01 00:00:00
abstract：:We have characterized the gravitropic response of inflorescence stems in Arabidopsis thaliana. When the inflorescence stems were placed horizontally, they curved upward about 90 degrees within 90 min in darkness at 23 degrees C, exhibiting strong negative gravitropism. Decapitated stem segments (without all flowers, f...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.110.3.933
更新日期：1996-03-01 00:00:00
abstract：:Air pollutants are known to cause visible leaf injury as well as impairment of photosynthetic CO(2) fixation. Here we evaluate whether the effects on photosynthesis are large enough to cause changes in the relative composition of stable carbon isotopes, delta(13)C, of plant tissue samples, and, if so, how the changes ...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.88.1.218
更新日期：1988-09-01 00:00:00
abstract：:A new conjugate, 1-(gamma-L-glutamylamino)cyclopropane-1-carboxylic acid (GACC), of the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) is identified. The only previously identified conjugate of ACC is 1-(malonylamino)cyclopropane-1-carboxylic acid (MACC). GACC, not MACC, was the major conjugate formed ...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.109.3.917
更新日期：1995-11-01 00:00:00
abstract：:The ambient light environment controls many aspects of plant development throughout a plant's life cycle. Such complex control is achieved because a key repressor of light signaling, the Arabidopsis (Arabidopsis thaliana) COP1/SPA E3 ubiquitin ligase causes the degradation of multiple regulators of endogenous developm...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.17.01207
更新日期：2018-02-01 00:00:00
abstract：:Understanding the distribution of elements within plant tissues is important across a range of fields in plant science. In this review, we examine synchrotron-based x-ray fluorescence microscopy (XFM) as an elemental imaging technique in plant sciences, considering both its historical and current uses as well as discu...
journal_title：Plant physiology
pub_type： 杂志文章,评审
doi：10.1104/pp.18.00759
更新日期：2018-10-01 00:00:00
abstract：:Enzymes dissociated from corn (hybrid B73 x Mo17) seedling cell walls by solutions of high ionic strength possess the capacity to degrade Avena caryopsis glucan. Inhibitor studies disclosed that both endo- and exoenzyme activities were involved and that the reaction sequence paralleled the autolytic solubilization of ...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.65.5.768
更新日期：1980-05-01 00:00:00
abstract：:Root iron mobilization genes are induced by iron deficiency downstream of an unknown signaling mechanism. The FER gene, encoding a basic helix-loop-helix domain protein and putative transcription factor, is required for induction of iron mobilization genes in roots of tomato (Lycopersicon esculentum). To study upstrea...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.104.054270
更新日期：2005-03-01 00:00:00
abstract：:Intact spinach and barley chloroplast normally incorporate (14)C-acetate into palmitate and oleate as the major (14)C fatty acids. Addition of nitrite markedly altered the relative patterns of the products with the appearance of stearate, a drop in oleate, but no marked change in palmitate. Arsenite greatly increased ...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.49.4.497
更新日期：1972-04-01 00:00:00
abstract：:Sucrose (Suc)-phosphate synthase (SPS) plays a crucial role in the synthesis of Suc in photosynthetic and nonphotosynthetic tissues. Several isoforms of SPS exist in dicotyledonous plants that can be grouped into the different families A, B, and C. To explore whether functional differences between the SPS gene familie...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.105.069468
更新日期：2005-11-01 00:00:00
abstract：:Flowering of the facultative long-day plant Arabidopsis is controlled by several endogenous and environmental factors, among them gibberellins (GAs) and day length. The promotion of flowering by long days involves an endogenous clock that interacts with light cues provided by the environment. Light, and specifically p...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.007625
更新日期：2002-12-01 00:00:00
abstract：:Plumeria (Plumeria rubra), well known for its brightly colored and fragrant flowers, emits a number of floral volatile organic compounds (VOCs). Plumeria flowers emit a total of 43 VOCs including nine phenylpropanoids/benzenoids, such as 2-phenylethanol (2PE), benzaldehyde, 2-phenylacetaldehyde (PAld), (E/Z)-phenylace...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.19.00098
更新日期：2019-05-01 00:00:00
abstract：:The ricinine content of etiolated seedlings of Ricinus communis increased nearly 12-fold over a 4-day period. In plants quinolinic acid is an intermediate in the de novo pathway for the synthesis of pyridine nucleotides. The only known enzyme in the de novo pathway for pyridine nucleotide biosynthesis, quinolinic acid...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.53.4.603
更新日期：1974-04-01 00:00:00
abstract：:The temporal sequence of development of the major proteins of seeds of soybean (Merr.) has been studied during development of cotyledons from flowering to maturity. A well-defined difference occurred in the times of appearance and the periods of maximum accumulation of alpha, alpha'-, and beta-subunits of betaconglyci...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.67.5.958
更新日期：1981-05-01 00:00:00
abstract：:The partitioning of noncyclic photosynthetic electron transport between net fixation of CO(2) and collective O(2)-dependent, dissipative processes such as photorespiration has been examined in intact leaf tissue from Nicotiana tabacum. The method involves simultaneous application of CO(2) exchange and pulse modulated ...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.90.4.1322
更新日期：1989-08-01 00:00:00
abstract：:Ca-mediated processes are known to be involved in transducing many developmental, hormonal, and environmental cues in plant cells. In this study, the role of Ca in the perception of anoxic stress signals by maize (Zea mays L. cv B73) roots was assessed by studying the effect of various Ca antagonists on the induction ...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.105.1.369
更新日期：1994-05-01 00:00:00
abstract：:In this study, we develop a mechanistic understanding of how temperature affects growth and photosynthesis in 10 geographically and physiologically diverse strains of Synechococcus spp. We found that Synechococcus spp. are able to regulate photochemistry over a range of temperatures by using state transitions and alte...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.113.221937
更新日期：2013-10-01 00:00:00
abstract：:Pathogenic isolates of Periconia circinata produce a host-specific toxin (PC-toxin) and cause a root and crown rot in susceptible genotypes of sorghum. Treatment with PC-toxin leads to selective development of disease symptoms and an increase in synthesis of a group of acidic, low molecular weight proteins only in sus...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.84.4.975
更新日期：1987-08-01 00:00:00
abstract：:Preliminary results from differential centrifugation experiments, washing treatments, and enrichment in linear sucrose gradients at a density of 1.09 grams per cubic centimeter all indicated that beta-glucosidase activity in corn root homogenates was associated with a membrane such as tonoplast. A subsequent sucrose d...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.76.4.861
更新日期：1984-12-01 00:00:00
abstract：:Purified glutamine synthetase from pea seedlings was most active with Mg(2+) as the metal activator, but Mn(2+) and Co(2+) were 45 to 60% and 30 to 45% as effective, respectively, when assayed at the optimal pH for each cation. The Mg(2+) saturation curve was quite sigmoid, and evidence indicates that MgATP is the act...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.54.5.773
更新日期：1974-11-01 00:00:00
abstract：:The intracellular distribution of the neurotoxin 2,4-diaminobutyric acid (DABA) in mature leaves of the perennial legume Lathyrus sylvestris L. var ;Lathco' (flatpea) was determined using subcellular fractions from mesophyll protoplasts. Chloroplasts contained about 15% of the cellular DABA. At least 75% of the DABA w...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.83.4.900
更新日期：1987-04-01 00:00:00
abstract：:Serial analysis of gene expression was used to profile transcript levels in Arabidopsis roots and assess their responses to 2,4,6-trinitrotoluene (TNT) exposure. SAGE libraries representing control and TNT-exposed seedling root transcripts were constructed, and each was sequenced to a depth of roughly 32,000 tags. Mor...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.103.028019
更新日期：2003-11-01 00:00:00
abstract：:Following dark adaptation, the response to irradiance of chlorophyll (Chl) fluorescence, the light-induced absorbance change around 820 nm (to measure reaction center Chl of photosystem I [PSI] P-700 oxidation), and CO2 fixation were examined in pea (Pisum sativum L.) leaves under a range of conditions. Initially, P-7...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.103.2.649
更新日期：1993-10-01 00:00:00
abstract：:The traditional method for determining compartmental analysis parameters relies on a visual selection of data points to be used for regression of data from each cellular compartment. This method is appropriate when the compartments are kinetically discrete and are easily discernible. However, where treatment effects o...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.76.4.913
更新日期：1984-12-01 00:00:00
abstract：:Experiments were conducted on Nicotiana tabacum, L. to study the relation in the grana among chlorophylls, carotenoids, and proteins. The effect of iron chlorosis on protein and pigment synthesis was studied at different stages of chlorosis using glycine-U-C(14). Pigments were separated by thin layer chromatography.Ch...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.41.3.415
更新日期：1966-03-01 00:00:00
abstract：:Treatment of bean (Phaseolus vulgaris L.) seedlings with low levels of salinity (50 or 100 millimolar NaCl) decreased the rate of light-induced leaf cell expansion in the primary leaves over a 3 day period. This decrease could be due to a reduction in one or both of the primary cellular growth parameters: wall exten...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.88.1.233
更新日期：1988-01-01 00:00:00
abstract：:Constitutive arginase (molecular weight 330,000) 920-fold purified from Evernia prunastri thallus, is activated by putrescine, l-ornithine, and agmatine with K(a) values of 2.7, 1.1, and 5.8 millimolar, respectively. Constitutive arginase is also activated by endogenous l-arginine, reaching its maximum activity at 16 ...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.76.4.1065
更新日期：1984-12-01 00:00:00
abstract：:2,4-Dichlorophenoxyacetic acid had a multiple effect on the development of indoleacetic acid oxidase isoenzymes in tobacco callus tissues (Nicotiana tabacum, cv. White Gold) cultured in vitro, and the development of these isoenzymes was differentially associated with growth promotion or inhibition depending on the con...
journal_title：Plant physiology
pub_type： 杂志文章
doi：10.1104/pp.49.6.957
更新日期：1972-06-01 00:00:00
