Mitochondrial protein lipoylation does not exclusively depend on the mtKAS pathway of de novo fatty acid synthesis in Arabidopsis.

Abstract:

:The photorespiratory Arabidopsis (Arabidopsis thaliana) mutant gld1 (now designated mtkas-1) is deficient in glycine decarboxylase (GDC) activity, but the exact nature of the genetic defect was not known. We have identified the mtkas-1 locus as gene At2g04540, which encodes beta-ketoacyl-[acyl carrier protein (ACP)] synthase (mtKAS), a key enzyme of the mitochondrial fatty acid synthetic system. One of its major products, octanoyl-ACP, is regarded as essential for the intramitochondrial lipoylation of several proteins including the H-protein subunit of GDC and the dihydrolipoamide acyltransferase (E2) subunits of two other essential multienzyme complexes, pyruvate dehydrogenase and alpha-ketoglutarate dehydrogenase. This view is in conflict with the fact that the mtkas-1 mutant and two allelic T-DNA knockout mutants grow well under nonphotorespiratory conditions. Although on a very low level, the mutants show residual lipoylation of H protein, indicating that the mutation does not lead to a full functional knockout of GDC. Lipoylation of the pyruvate dehydrogenase and alpha-ketoglutarate dehydrogenase E2 subunits is distinctly less reduced than that of H protein in leaves and remains unaffected from the mtKAS knockout in roots. These data suggest that mitochondrial protein lipoylation does not exclusively depend on the mtKAS pathway of lipoate biosynthesis in leaves and may occur independently of this pathway in roots.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Ewald R,Kolukisaoglu U,Bauwe U,Mikkat S,Bauwe H

doi

10.1104/pp.107.104000

subject

Has Abstract

pub_date

2007-09-01 00:00:00

pages

41-8

issue

1

eissn

0032-0889

issn

1532-2548

pii

pp.107.104000

journal_volume

145

pub_type

杂志文章
  • Protein and RNA Synthesizing Activities during Sexual Process of Heterothallic Closterium.

    abstract::Protein and RNA synthesizing activities increased markedly during the mating process and decreased during the maturation stage of zygotes in heterothallic strains of Closterium peracerosum-strigosum-littoale, KAS-4-29 (mating-type minus) and KAS -4-30 (mating-type plus) and a homothallic Closterium acerosum. Different...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.78.4.696

    authors: Kato A,Sasaki K

    更新日期:1985-08-01 00:00:00

  • Transient accumulation of asparagine in sycamore cells after a long period of sucrose starvation.

    abstract::The mobilization of stored carbohydrates (sucrose and starch) and proteins during sucrose starvation was studied with sycamore (Acer pseudoplatanus L.) cells. When almost all the intracellular carbohydrate pools had disappeared, the cell protein content declined progressively whereas asparagine determined by either (1...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.94.2.717

    authors: Genix P,Bligny R,Martin JB,Douce R

    更新日期:1990-10-01 00:00:00

  • Nitrogen Metabolism in Soybean Tissue Culture: II. Urea Utilization and Urease Synthesis Require Ni.

    abstract::Potassium citrate (10 mM, pH 6) inhibits the growth of cultured (Glycine max L.) cells when urea is the sole nitrogen source. Ureadependent citrate toxicity is overcome by three separate additions to the growth medium: (a) NH(4)Cl (20 mM); (b) high levels of MgCl(2) (10 mM) or CaCl(2) (5-10 mM); (c) low levels of NiSO...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.59.5.827

    authors: Polacco JC

    更新日期:1977-05-01 00:00:00

  • CO(2) Enhancement of Growth and Photosynthesis in Rice (Oryza sativa) : Modification by Increased Ultraviolet-B Radiation.

    abstract::Two cultivars of rice (Oryza sativa L.) IR-36 and Fujiyama-5 were grown at ambient (360 microbars) and elevated CO(2) (660 microbars) from germination through reproduction in unshaded greenhouses at the Duke University Phytotron. Growth at elevated CO(2) resulted in significant decreases in nighttime respiration and i...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.99.2.473

    authors: Ziska LH,Teramura AH

    更新日期:1992-06-01 00:00:00

  • Transgenic tobacco and Arabidopsis plants expressing the two multifunctional sorghum cytochrome P450 enzymes, CYP79A1 and CYP71E1, are cyanogenic and accumulate metabolites derived from intermediates in Dhurrin biosynthesis.

    abstract::Novel cyanogenic plants have been generated by the simultaneous expression of the two multifunctional sorghum (Sorghum bicolor [L.] Moench) cytochrome P450 enzymes CYP79A1 and CYP71E1 in tobacco (Nicotiana tabacum cv Xanthi) and Arabidopsis under the regulation of the constitutive 35S promoter. CYP79A1 and CYP71E1 cat...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.123.4.1437

    authors: Bak S,Olsen CE,Halkier BA,Møller BL

    更新日期:2000-08-01 00:00:00

  • Impact of altered gibberellin metabolism on biomass accumulation, lignin biosynthesis, and photosynthesis in transgenic tobacco plants.

    abstract::Gibberellins (GAs) are involved in regulation of many aspects during plant development. To investigate the impact of altered GA levels on plant growth and metabolism, transgenic tobacco (Nicotiana tabacum) plants have been engineered to express either a GA20-oxidase (AtGA20-ox) or a GA2-oxidase (AtGA2-ox) gene from Ar...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.036988

    authors: Biemelt S,Tschiersch H,Sonnewald U

    更新日期:2004-05-01 00:00:00

  • Clonal variation for tolerance to polyethylene glycol-induced water stress in cultured tomato cells.

    abstract::Cell clones were isolated from a population of cultured tomato (Lycopersicon esculentum Mill cv VFNT-cherry) cells and their tolerance to polyethylene glycol (PEG)-induced water stress was measured. Considerable variation for tolerance among the clones was found. Tolerance differences between clones appeared to be spo...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.72.3.645

    authors: Handa AK,Bressan RA,Handa S,Hasegawa PM

    更新日期:1983-07-01 00:00:00

  • Characterization of the branched-chain amino acid aminotransferase enzyme family in tomato.

    abstract::Branched-chain amino acids (BCAAs) are synthesized in plants from branched-chain keto acids, but their metabolism is not completely understood. The interface of BCAA metabolism lies with branched-chain aminotransferases (BCAT) that catalyze both the last anabolic step and the first catabolic step. In this study, six B...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.154922

    authors: Maloney GS,Kochevenko A,Tieman DM,Tohge T,Krieger U,Zamir D,Taylor MG,Fernie AR,Klee HJ

    更新日期:2010-07-01 00:00:00

  • Kinetic Variance of Ribulose-1,5-bisphosphate Carboxylase/Oxygenase Isolated from Diverse Taxonomic Sources : II. Analysis by Two Dual Label Methods.

    abstract::Two dual label methods were used to investigate kinetic variability of ribulose 1,5-bisphosphate (RuBP) carboxylase/oxygenase (EC 4.1.1.39). In addition to using [1-(14)C,5-(3)H]RuBP (method 1), we describe here the detailed assay with (14)CO(2) and [5-(3)H]RuBP (method 2), which generates [(3)H,(14)C]3-phosphoglyceri...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.75.3.645

    authors: Kent SS,Tomany MJ

    更新日期:1984-07-01 00:00:00

  • Snakin-2, an antimicrobial peptide from potato whose gene is locally induced by wounding and responds to pathogen infection.

    abstract::The peptide snakin-2 (StSN2) has been isolated from potato (Solanum tuberosum cv Jaerla) tubers and found to be active (EC(50) = 1-20 microM) against fungal and bacterial plant pathogens. It causes a rapid aggregation of both Gram-positive and Gram-negative bacteria. The corresponding StSN2 cDNA encodes a signal seque...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.010685

    authors: Berrocal-Lobo M,Segura A,Moreno M,López G,García-Olmedo F,Molina A

    更新日期:2002-03-01 00:00:00

  • Isolation from sugar beet cell walls of arabinan oligosaccharides esterified by two ferulic acid monomers.

    abstract::Side chains of sugar beet (Beta vulgaris) pectins, which are mainly composed of arabinose (Ara) and galactose (Gal) residues, are esterified by ferulic acid units. Enzymatic hydrolysis of beet cell walls yielded several feruloylated oligosaccharides, which were separated by hydrophobic interaction chromatography. Two ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.035311

    authors: Levigne SV,Ralet MC,Quéméner BC,Pollet BN,Lapierre C,Thibault JF

    更新日期:2004-03-01 00:00:00

  • Temperature response of isoprene emission in vivo reflects a combined effect of substrate limitations and isoprene synthase activity: a kinetic analysis.

    abstract::The responses of isoprene emission rate to temperature are characterized by complex time-dependent behaviors that are currently not entirely understood. To gain insight into the temperature dependencies of isoprene emission, we studied steady-state and transient responses of isoprene emission from hybrid aspen (Populu...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.162081

    authors: Rasulov B,Hüve K,Bichele I,Laisk A,Niinemets U

    更新日期:2010-11-01 00:00:00

  • Role of asparagine in the photorespiratory nitrogen metabolism of pea leaves.

    abstract::In pea leaves, much of the metabolism of imported asparagine is by transamination. This activity was previously shown to be localized in the peroxisomes, suggesting a possible connection between asparagine and photorespiratory nitrogen metabolism. This was investigated by examination of the transfer of (15)N from the ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.78.2.334

    authors: Ta TC,Joy KW,Ireland RJ

    更新日期:1985-06-01 00:00:00

  • Arabidopsis seedling growth response and recovery to ethylene. A kinetic analysis.

    abstract::Responses to the plant hormone ethylene are mediated by a family of five receptors in Arabidopsis that act in the absence of ethylene as negative regulators of response pathways. In this study, we examined the rapid kinetics of growth inhibition by ethylene and growth recovery after ethylene withdrawal in hypocotyls o...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.050369

    authors: Binder BM,O'malley RC,Wang W,Moore JM,Parks BM,Spalding EP,Bleecker AB

    更新日期:2004-10-01 00:00:00

  • Architecture-based multiscale computational modeling of plant cell wall mechanics to examine the hydrogen-bonding hypothesis of the cell wall network structure model.

    abstract::A primary plant cell wall network was computationally modeled using the finite element approach to study the hypothesis of hemicellulose (HC) tethering with the cellulose microfibrils (CMFs) as one of the major load-bearing mechanisms of the growing cell wall. A computational primary cell wall network fragment (10 × 1...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.201228

    authors: Yi H,Puri VM

    更新日期:2012-11-01 00:00:00

  • MEKK1 is required for flg22-induced MPK4 activation in Arabidopsis plants.

    abstract::The Arabidopsis (Arabidopsis thaliana) gene MEKK1 encodes a mitogen-activated protein kinase kinase kinase that has been implicated in the activation of the map kinases MPK3 and MPK6 in response to the flagellin elicitor peptide flg22. In this study, analysis of plants carrying T-DNA knockout alleles indicated that ME...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.091389

    authors: Suarez-Rodriguez MC,Adams-Phillips L,Liu Y,Wang H,Su SH,Jester PJ,Zhang S,Bent AF,Krysan PJ

    更新日期:2007-02-01 00:00:00

  • Evidence That IAA Conjugates Are Slow-Release Sources of Free IAA in Plant Tissues.

    abstract::Evidence that indoleacetic acid (IAA) conjugates are metabolized via enzyme-catalyzed hydrolysis to free IAA and that their biological activities are related to the rates at which they are hydrolyzed by the tissue is presented. These conclusions are based on the following observations. Slow but continuous decarboxylat...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.68.6.1424

    authors: Hangarter RP,Good NE

    更新日期:1981-12-01 00:00:00

  • A phosphothreonine residue at the C-terminal end of the plasma membrane H+-ATPase is protected by fusicoccin-induced 14-3-3 binding.

    abstract::We have isolated the plasma membrane H+-ATPase in a phosphorylated form from spinach (Spinacia oleracea L.) leaf tissue incubated with fusicoccin, a fungal toxin that induces irreversible binding of 14-3-3 protein to the C terminus of the H+-ATPase, thus activating H+ pumping. We have identified threonine-948, the sec...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.118.2.551

    authors: Olsson A,Svennelid F,Ek B,Sommarin M,Larsson C

    更新日期:1998-10-01 00:00:00

  • Kinase MPK17 and the Peroxisome Division Factor PMD1 Influence Salt-induced Peroxisome Proliferation.

    abstract::Peroxisomes are small organelles that house many oxidative reactions. Peroxisome proliferation is induced under multiple stress conditions, including salt stress; however, factors regulating this process are not well defined. We have identified a role for Arabidopsis (Arabidopsis thaliana) MAP KINASE17 (MPK17) in affe...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.17.01019

    authors: Frick EM,Strader LC

    更新日期:2018-01-01 00:00:00

  • Peridinin-Chlorophyll a Proteins of the Dinoflagellate Amphidinium carterae (Plymouth 450).

    abstract::The marine dinoflagellate Amphidinium carterae (Plymouth 450) releases several water-soluble peridinin-chlorophyll a proteins after freezethawing. These chromoproteins have a molecular weight of 39.2 x 10(3) and are comprised of noncovalently bound peridinin and chlorophyll a and a nonoligomeric protein. They have dis...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.57.2.297

    authors: Haxo FT,Kycia JH,Somers GF,Bennett A,Siegelman HW

    更新日期:1976-02-01 00:00:00

  • Effects of low concentrations of o(3) on net photosynthesis, dark respiration, and chlorophyll contents in aging hybrid poplar leaves.

    abstract::Chronic exposure of hybrid poplar (Populus deltoides x trichocarpa) plants to low concentrations of ozone had negative impact upon net photosynthetic capacity, dark respiration, and leaf chlorophyll contents. Exposure to as much as 0.20 microliters per liter O(3) had no immediate effect on net photosynthesis (P(n)), b...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.73.2.291

    authors: Reich PB

    更新日期:1983-10-01 00:00:00

  • Chloroplast 2010: a database for large-scale phenotypic screening of Arabidopsis mutants.

    abstract::Large-scale phenotypic screening presents challenges and opportunities not encountered in typical forward or reverse genetics projects. We describe a modular database and laboratory information management system that was implemented in support of the Chloroplast 2010 Project, an Arabidopsis (Arabidopsis thaliana) reve...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.170118

    authors: Lu Y,Savage LJ,Larson MD,Wilkerson CG,Last RL

    更新日期:2011-04-01 00:00:00

  • Inhibition of the Hill Reaction by Tris and Restoration by Electron Donation to Photosystem II.

    abstract::Experiments in which chloroplasts were washed with tris and tricine buffers at different pH's indicated that the non-protonated (uncharged) form of tris was inhibitory to the Hill reaction while the protonated form of tris and the zwitterionic forms of tricine were non-inhibitory. Buffers analogous to tris and tricine...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.44.3.435

    authors: Yamashita T,Butler WL

    更新日期:1969-03-01 00:00:00

  • The Root Foraging Response under Low Nitrogen Depends on DWARF1-Mediated Brassinosteroid Biosynthesis.

    abstract::Root developmental plasticity enables plants to adapt to limiting or fluctuating nutrient conditions in the soil. When grown under nitrogen (N) deficiency, plants develop a more exploratory root system by increasing primary and lateral root length. However, mechanisms underlying this so-called foraging response remain...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.20.00440

    authors: Jia Z,Giehl RFH,von Wirén N

    更新日期:2020-07-01 00:00:00

  • Genotypic variability in vulnerability of leaf xylem to cavitation in water-stressed and well-irrigated sugarcane.

    abstract::Genotypic variability in vulnerability of leaf xylem to water-stress-induced cavitation was determined in four sugarcane (Saccharum sp.) clones using detached leaf segments in a hydraulic conductivity apparatus. Vulnerability curves were constructed by plotting the percentage of maximum conductivity versus leaf water ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.100.2.1020

    authors: Neufeld HS,Grantz DA,Meinzer FC,Goldstein G,Crisosto GM,Crisosto C

    更新日期:1992-10-01 00:00:00

  • Function of Rhizodermal Transfer Cells in the Fe Stress Response Mechanism of Capsicum annuum L.

    abstract::A variety of red pepper (Capsicum annuum L., cv Yaglik) responds to Fe deficiency stress with simultaneously enhanced H(+) extrusion, reduction of ferric ions and synthesis of malic and citric acid in a swollen subapical root zone densely covered with root hairs. It is demonstrated that these stress responses temporal...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.82.2.511

    authors: Landsberg EC

    更新日期:1986-10-01 00:00:00

  • Nuclear, Virescent Mutants of Zea mays L. with High Levels of Chlorophyll (a/b) Light-Harvesting Complex during Thylakoid Assembly.

    abstract::We have found nuclear, recessive mutants in Zea mays L. where assembly of the major chlorophyll (a/b) light-harvesting complex (LHC) was not delayed relative to most other thylakoid protein complexes during thylakoid biogenesis. This contrasts with the normal development of maize chloroplasts (NR Baker, R Leech 1977 P...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.77.4.795

    authors: Polacco ML,Chang MT,Neuffer MG

    更新日期:1985-04-01 00:00:00

  • Does the Arabidopsis proton gradient regulation5 Mutant Leak Protons from the Thylakoid Membrane?

    abstract::Despite generating an obvious mutant phenotype, whether the Arabidopsis (Arabidopsis thaliana) proton gradient regulation5 (pgr5) mutation influences cyclic electron transport (CET) around PSI is a topic of debate. Results of electrochromic shift analysis show that proton conductivity across the thylakoid membrane (gH...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.20.00850

    authors: Yamamoto H,Shikanai T

    更新日期:2020-09-01 00:00:00

  • Molecular characterization of oat seed globulins.

    abstract::We have isolated full-length cDNA clones that encode oat (Avena sativa) seed storage globulin mRNAs from a cDNA library in the expression vector lambda gtll. The longest of these clones, pOG2, has an 1840-base pair insert that encodes a complete precursor subunit with a signal peptide of 24 amino acids followed by an ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.87.3.698

    authors: Shotwell MA,Afonso C,Davies E,Chesnut RS,Larkins BA

    更新日期:1988-07-01 00:00:00

  • Pyrimidine Metabolism in Lemna minor: I. Functional Compartmentation of Chloroplast Pyrimidine Metabolism in a Higher Plant.

    abstract::Cytidine deoxyriboside (Cdr), uridine deoxyriboside (Udr), and guanosine deoxyriboside (Gdr), induce quantitative bleaching of the fronds of Lemna minor (duckweed) during growth in continuous light on photoheterotrophic medium. Cdr-induced bleaching is not accompanied by a reduction in frond multiplication rate, but U...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.61.6.989

    authors: Frick H

    更新日期:1978-06-01 00:00:00