Techniques for the Analysis of Protein-Protein Interactions in Vivo.

abstract：:The degree of tubulin polymerization in cotton (Gossypium hirsutum L. cv Acala) cotyledonary tissue was estimated by radioimmunoassay which measured the amount of a tubulin-like factor. It was assumed that the release of this tubulin-like factor indicated depolymerization of microtubules. Exposure to chilling resulted...

journal_title：Plant physiology

authors： Rikin A,Atsmon D,Gitler C

更新日期：1983-04-01 00:00:00

abstract：:(31)P NMR spectroscopy was used to study in vivo the symbiotic state established between soybean (Glycine max [L.] Merr. cv Williams) and Bradyrhizobium japonicum (USDA 110 and 138). Different experimental conditions were used to maintain perfused, respiring detached or attached nodules in an NMR magnet. The pH of the...

journal_title：Plant physiology

authors： Rolin DB,Boswell RT,Sloger C,Tu SI,Pfeffer PE

更新日期：1989-04-01 00:00:00

abstract：:Prolonged growth of cell cultures of sycamore (Acer pseudoplatanus L.) on agar medium containing myo-inositol-2-(3)H resulted in incorporation of label predominately into uronosyl and pentosyl units of cell wall polysaccharides. Procedures normally used to distinguish between pectic substance and hemicellulose yielded...

journal_title：Plant physiology

authors： Roberts RM,Loewus F

更新日期：1966-11-01 00:00:00

abstract：:We report the first complete purifications of the cytosolic and plastid isozymes of triose phosphate isomerase (TPI; EC 5.3.1.1) from higher plants including spinach (Spinacia oleracea), lettuce (Lactuca sativa), and celery (Apium graveolens). Both isozymes are composed of two isosubunits with approximate molecular we...

journal_title：Plant physiology

authors： Pichersky E,Gottlieb LD

更新日期：1984-02-01 00:00:00

abstract：:In culture, the phytopathogenic fungus Phytophthora cryptogea secretes a protein which elicits hypersensitive-like necroses and protects tobacco plants against invasion by the pathogen Phytophthora parasitica var. nicotianae. This protein, named cryptogein, has been purified and its amino acid sequence determined. In ...

journal_title：Plant physiology

authors： Blein JP,Milat ML,Ricci P

更新日期：1991-02-01 00:00:00

abstract：:Experiments were performed to confirm that the aldimine bond formation is a spontaneous reaction, because attempts to find an enzyme catalyzing the last decisive step in betaxanthin biosynthesis, the aldimine formation, failed. Feeding different amino acids to betalain-forming hairy root cultures of yellow beet (Beta ...

journal_title：Plant physiology

authors： Schliemann W,Kobayashi N,Strack D

更新日期：1999-04-01 00:00:00

abstract：:Nonautotrophic CO(2) metabolism in Opuntia echinocarpa roots was studied with techniques of manometry and radiometry. The roots were grown in a one-quarter strength nutrient solution for several days; the distal 2 cm was used for physiological studies. The roots assimilated significant quantities of (14)CO(2) and appe...

journal_title：Plant physiology

authors： Ting IP,Dugger WM

更新日期：1966-03-01 00:00:00

abstract：:The promoter of phytoene synthase, the first specific enzyme of carotenoid biosynthesis, shows two main regulatory regions: a G-box-containing region located near the TATA box, and a TATA box distal region containing the cis-acting element ATCTA, which mediates strong basal promoter activity. This second element was a...

journal_title：Plant physiology

authors： Welsch R,Maass D,Voegel T,Dellapenna D,Beyer P

更新日期：2007-11-01 00:00:00

abstract：:Evidence is presented to support the hypothesis that serine, rather than 3-phosphoglycerate of the Calvin cycle, is a precursor of the tricarboxylic acid cycle during photosynthesis by the higher plant, Vicia faba. Identification of the serine intermediate is based upon a unique C(1) > C(2) > C(3) isotope distribution...

journal_title：Plant physiology

authors： Kent SS,Pinkerton FD,Strobel GA

更新日期：1974-03-01 00:00:00

abstract：:Cinnamate-4-hydroxylase (C4H) is the first Cyt P450-dependent monooxygenase of the phenylpropanoid pathway. To study the expression of this gene in Arabidopsis thaliana, a C4H cDNA clone from the Arabidopsis expressed sequence tag database was identified and used to isolate its corresponding genomic clone. The entire ...

journal_title：Plant physiology

authors： Bell-Lelong DA,Cusumano JC,Meyer K,Chapple C

更新日期：1997-03-01 00:00:00

abstract：:Light stimulated the activation of ribulosebisphosphate carboxylase/oxygenase (rubisco) in a buffered lysed chloroplast system in the presence of saturating concentrations of ATP. This indicates a role for light in the rubisco activase activation system in addition to the previously identified requirement for the synt...

journal_title：Plant physiology

authors： Campbell WJ,Ogren WL

更新日期：1990-01-01 00:00:00

abstract：:Phenol inhibits the Hill reaction with several Hill oxidants and the accompanying non-cyclic phosphorylation. It inhibits also pseudocyclic and cyclic phosphorylation. Partial reactions which are dependent on cyclic electron flow are inhibited too, but electron flow from ascorbate via dichlorophenolindophenol to TPN i...

journal_title：Plant physiology

authors： Neumann J,Drechsler Z

更新日期：1967-04-01 00:00:00

abstract：:Callus cultures of peanut (Arachis hypogaea L. cv Valencia Tennessee Red) cotyledons grown on Linsmaier and Skoog medium containing normal levels of auxin and cytokinin do not synthesize either 4-methyl-eneglutamic acid or 4-methyleneglutamine, which nonprotein amino acids are normally found in significant amounts in ...

journal_title：Plant physiology

authors： Winter HC,Dekker EE

更新日期：1984-09-01 00:00:00

abstract：:Induction of ferric reductase activity in dicots and nongrass monocots is a well-recognized response to Fe deficiency. Recent evidence has shown that Cu deficiency also induces plasma membrane Fe reduction. In this study we investigated whether other nutrient deficiencies could also induce ferric reductase activity in...

journal_title：Plant physiology

authors： Cohen CK,Norvell WA,Kochian LV

更新日期：1997-07-01 00:00:00

abstract：:The mechanism of sucrose transport into vacuoles isolated from leaf tissue has been studied only in barley (Hordeum vulgare) mesophyll cells. In this tissue, sucrose transport was reported to be a facilitated diffusion. We have observed a facilitated diffusion of sucrose into vacuoles isolated from this tissue. Howeve...

journal_title：Plant physiology

authors： De Leon JL,Daie J,Wyse R

更新日期：1988-10-01 00:00:00

abstract：:A model which predicts total photosynthetic electron flow from a linear regression of the relationship between corrected steady-state quantum yield and nonphotochemical quenching (E Weis, JA Berry [1987] Biochem Biophys Acta 894: 198-208) was formulated for N-limited cells of the green alga Selenastrum minutum. Unlike...

journal_title：Plant physiology

authors： Holmes JJ,Weger HG,Turpin DH

更新日期：1989-09-01 00:00:00

abstract：:The starch-granule-bound starch synthase from Zea mays has been solubilized with a recovery of between 50 and 84%. Chromatography of the solubilized enzyme on DEAE-Sepharose resolves two fractions of activity which may be distinguished by their response to citrate. Neither solubilized isoenzyme displays any significan...

journal_title：Plant physiology

authors： Macdonald FD,Preiss J

更新日期：1983-09-01 00:00:00

abstract：:During the early stages of seed development, Arabidopsis (Arabidopsis thaliana) endosperm is syncytial and proliferates rapidly through repeated rounds of mitosis without cytokinesis. This stage of endosperm development is important in determining final seed size and is a model for studying aspects of cellular and mol...

journal_title：Plant physiology

authors： Day RC,Herridge RP,Ambrose BA,Macknight RC

更新日期：2008-12-01 00:00:00

abstract：:We used three-dimensional structured illumination microscopy (3D-SIM) to obtain subdiffraction ("super-resolution") images of plasmodesmata (PD) expressing a green fluorescent protein-tagged viral movement protein (MP) in tobacco (Nicotiana tabacum). In leaf parenchyma cells, we were able to resolve individual compone...

journal_title：Plant physiology

authors： Fitzgibbon J,Bell K,King E,Oparka K

更新日期：2010-08-01 00:00:00

abstract：:ELIPs (early light-induced proteins) are thylakoid proteins transiently induced during greening of etiolated seedlings and during exposure to high light stress conditions. This expression pattern suggests that these proteins may be involved in the protection of the photosynthetic apparatus against photooxidative damag...

journal_title：Plant physiology

authors： Rossini S,Casazza AP,Engelmann EC,Havaux M,Jennings RC,Soave C

更新日期：2006-08-01 00:00:00

abstract：:The temporal sequence of development of the major proteins of seeds of soybean (Merr.) has been studied during development of cotyledons from flowering to maturity. A well-defined difference occurred in the times of appearance and the periods of maximum accumulation of alpha, alpha'-, and beta-subunits of betaconglyci...

journal_title：Plant physiology

authors： Gayler KR,Sykes GE

更新日期：1981-05-01 00:00:00

abstract：:Mevalonic acid-2-(14)C was readily incorporated into the free, esterified, and glycosidic sterol fractions of tobacco (Nicotiana tabacum L. var. Burley 21) seedlings. The time course of mevalonic acid-2-(14)C incorporation was different for the various individual sterols. Campesterol and sitosterol (group I) became ra...

journal_title：Plant physiology

authors： Bush PB,Grunwald C

更新日期：1973-01-01 00:00:00

abstract：:To investigate the relation between cell division and expansion in the regulation of organ growth rate, we used Arabidopsis thaliana primary roots grown vertically at 20 degreesC with an elongation rate that increased steadily during the first 14 d after germination. We measured spatial profiles of longitudinal veloci...

journal_title：Plant physiology

authors： Beemster GT,Baskin TI

更新日期：1998-04-01 00:00:00

abstract：:Regulation of photosynthetic gene expression by plastid-encoded RNA polymerase (PEP) is essential for chloroplast development. The activity of PEP largely relies on at least 12 PEP-associated proteins (PAPs) encoded in the nuclear genome of plant cells. A recent model proposed that these PAPs regulate the establishmen...

journal_title：Plant physiology

authors： Chang SH,Lee S,Um TY,Kim JK,Do Choi Y,Jang G

更新日期：2017-05-01 00:00:00

abstract：:In pea leaves, much of the metabolism of imported asparagine is by transamination. This activity was previously shown to be localized in the peroxisomes, suggesting a possible connection between asparagine and photorespiratory nitrogen metabolism. This was investigated by examination of the transfer of (15)N from the ...

journal_title：Plant physiology

authors： Ta TC,Joy KW,Ireland RJ

更新日期：1985-06-01 00:00:00

abstract：:The rate of O(2) uptake by excised Samanea pulvini oscillates with a circadian rhythm during 52 hours of darkness. Rates of respiration increase during pulvinar opening and decrease prior to and during closure, consistent with the concept that opening requires a greater expenditure of energy. Externally supplied sucro...

journal_title：Plant physiology

authors： Satter RL

更新日期：1979-09-01 00:00:00

abstract：:Leaves of Kalanchoë pinnata were exposed in the dark to air (allowing the fixation of CO(2) into malic acid) or 2% O(2), 0% CO(2) (preventing malic acid accumulation). They were then exposed to bright light in the presence or absence of external CO(2) and light dependent inhibition of photosynthetic properties assesse...

journal_title：Plant physiology

authors： Adams WW,Osmond CB

更新日期：1988-01-01 00:00:00

abstract：:The Aurora B kinase, encoded by the AURORA 3 (AUR3) gene in Arabidopsis (Arabidopsis thaliana), is a key regulator of cell division in all eukaryotes. Aurora B has at least two central functions during cell division; it is essential for the correct, i.e. balanced, segregation of chromosomes in mitosis and meiosis by c...

journal_title：Plant physiology

authors： Komaki S,Takeuchi H,Hamamura Y,Heese M,Hashimoto T,Schnittger A

更新日期：2020-08-01 00:00:00

abstract：:Recent structure-function analysis of heterologously expressed K+-selective inward-rectifying channels (KIRCs) from plants has revealed that external protons can have opposite effects on different members of the same gene family. An important question is how the diverse response of KIRCs to apoplastic pH is reflected ...

journal_title：Plant physiology

authors： Amtmann A,Jelitto TC,Sanders D

更新日期：1999-05-01 00:00:00

abstract：:Correlating measurements from differential scanning calorimetry, freeze-fracture freeze-etch electron microscopy, and survival of twigs after two-step cooling experiments, we provide strong evidence that winter-hardened Populus balsamifera v. virginiana (Sarg.) resists the stresses of freezing below -28 degrees C by a...

journal_title：Plant physiology

authors： Hirsh AG,Williams RJ,Meryman HT

更新日期：1985-09-01 00:00:00