Abstract:
:To study the role of the plastidial alpha-glucan phosphorylase in starch metabolism in the leaves of Arabidopsis, two independent mutant lines containing T-DNA insertions within the phosphorylase gene were identified. Both insertions eliminate the activity of the plastidial alpha-glucan phosphorylase. Measurement of other enzymes of starch metabolism reveals only minor changes compared with the wild type. The loss of plastidial alpha-glucan phosphorylase does not cause a significant change in the total accumulation of starch during the day or its remobilization at night. Starch structure and composition are unaltered. However, mutant plants display lesions on their leaves that are not seen on wild-type plants, and mesophyll cells bordering the lesions accumulate high levels of starch. Lesion formation is abolished by growing plants under 100% humidity in still air, but subsequent transfer to circulating air with lower humidity causes extensive wilting in the mutant leaves. Wilted sectors die, causing large lesions that are bordered by starch-accumulating cells. Similar lesions are caused by the application of acute salt stress to mature plants. We conclude that plastidial phosphorylase is not required for the degradation of starch, but that it plays a role in the capacity of the leaf lamina to endure a transient water deficit.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Zeeman SC,Thorneycroft D,Schupp N,Chapple A,Weck M,Dunstan H,Haldimann P,Bechtold N,Smith AM,Smith SMdoi
10.1104/pp.103.032631subject
Has Abstractpub_date
2004-06-01 00:00:00pages
849-58issue
2eissn
0032-0889issn
1532-2548pii
pp.103.032631journal_volume
135pub_type
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