Selection for Improved Energy Use Efficiency and Drought Tolerance in Canola Results in Distinct Transcriptome and Epigenome Changes.

Abstract:

:To increase both the yield potential and stability of crops, integrated breeding strategies are used that have mostly a direct genetic basis, but the utility of epigenetics to improve complex traits is unclear. A better understanding of the status of the epigenome and its contribution to agronomic performance would help in developing approaches to incorporate the epigenetic component of complex traits into breeding programs. Starting from isogenic canola (Brassica napus) lines, epilines were generated by selecting, repeatedly for three generations, for increased energy use efficiency and drought tolerance. These epilines had an enhanced energy use efficiency, drought tolerance, and nitrogen use efficiency. Transcriptome analysis of the epilines and a line selected for its energy use efficiency solely revealed common differentially expressed genes related to the onset of stress tolerance-regulating signaling events. Genes related to responses to salt, osmotic, abscisic acid, and drought treatments were specifically differentially expressed in the drought-tolerant epilines. The status of the epigenome, scored as differential trimethylation of lysine-4 of histone 3, further supported the phenotype by targeting drought-responsive genes and facilitating the transcription of the differentially expressed genes. From these results, we conclude that the canola epigenome can be shaped by selection to increase energy use efficiency and stress tolerance. Hence, these findings warrant the further development of strategies to incorporate epigenetics into breeding.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Verkest A,Byzova M,Martens C,Willems P,Verwulgen T,Slabbinck B,Rombaut D,Van de Velde J,Vandepoele K,Standaert E,Peeters M,Van Lijsebettens M,Van Breusegem F,De Block M

doi

10.1104/pp.15.00155

subject

Has Abstract

pub_date

2015-08-01 00:00:00

pages

1338-50

issue

4

eissn

0032-0889

issn

1532-2548

pii

pp.15.00155

journal_volume

168

pub_type

杂志文章
  • On the Role of Stored mRNA in Protein Synthesis in Embryonic Axes of Germinating Vigna unguiculata Seeds.

    abstract::Polyadenylated (poly A(+)) RNAs were prepared from both dry and incubated embryonic axes of Vigna unguiculata seeds and were translated by a wheat germ translation system. Analysis with gel electrophoresis and fluorography showed that translation products of poly A(+) RNA from dry embryonic axes were nearly the same a...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.79.2.327

    authors: Suzuki Y,Minamikawa T

    更新日期:1985-10-01 00:00:00

  • Anomalous nonstoichiometry detected by dual label analysis of ribulose 1,5-bisphosphate carboxylase.

    abstract::When ribulose-1,5-bisphosphate carboxylase is assayed under N(2) using [(3)H]ribulose 1,5-bisphosphate and (14)CO(2), [(3)H]3-phosphoglycerate and [(14)C]3-phosphoglycerate are produced in nonstoichiometric amounts in a ratio which approaches 7 at low concentrations of CO(2) (2 micromolar) assuming a 1:1 ratio at V(ma...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.80.4.1055

    authors: Tomany MJ,Kent SS

    更新日期:1986-04-01 00:00:00

  • Aspartate Aminotransferase in Alfalfa Root Nodules : II. Immunological Distinction between Two Forms of the Enzyme.

    abstract::Aspartate aminotransferase (AAT), a key enzyme in the biosynthesis of aspartate and asparagine, occurs as two forms in alfalfa (Medicago sativa L.), AAT-1 and AAT-2. Both forms were purified to near homogeneity, and high titer polyclonal antibodies produced to the native proteins. Alfalfa AAT-1 was purified from root ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.93.2.603

    authors: Farnham MW,Miller SS,Griffith SM,Vance CP

    更新日期:1990-06-01 00:00:00

  • Characterization of a new antifungal chitin-binding peptide from sugar beet leaves.

    abstract::The intercellular washing fluid (IWF) from leaves of sugar beet (Beta vulgaris L.) contains a number of proteins exhibiting in vitro antifungal activity against the devastating leaf pathogen Cercospora beticola (Sacc.). Among these, a potent antifungal peptide, designated IWF4, was identified. The 30-amino-acid residu...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.1.83

    authors: Nielsen KK,Nielsen JE,Madrid SM,Mikkelsen JD

    更新日期:1997-01-01 00:00:00

  • Enhancement of wound-induced ethylene synthesis by ethylene in preclimacteric cantaloupe.

    abstract::Although intact fruits of unripe cantaloupe (Cucumis melo L.) produce very little ethylene, a massive increase in ethylene production occurred in response to excision. The evidence indicates that this wound ethylene is produced from methionine via 1-aminocyclopropanecarboxylic acid (ACC) as in ripening fruits. Excisio...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.69.2.317

    authors: Hoffman NE,Yang SF

    更新日期:1982-02-01 00:00:00

  • Enhancement of polyribosome formation and RNA synthesis of gibberellic Acid in wounded potato tuber tissue.

    abstract::As part of a more detailed study on plant tumorigenesis, the action of gibberellic acid (GA(3)) in wounded potato tuber tissues as a model system has been evaluated. GA(3) stimulates total RNA synthesis in wounded tissues, the optimal concentration being 0.1 micromolar. The responsiveness of the tissue toward the horm...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.64.5.863

    authors: Wielgat B

    更新日期:1979-11-01 00:00:00

  • Identification of a specific isoform of tomato lipoxygenase (TomloxC) involved in the generation of fatty acid-derived flavor compounds.

    abstract::There are at least five lipoxygenases (TomloxA, TomloxB, TomloxC, TomloxD, and TomloxE) present in tomato (Lycopersicon esculentum Mill.) fruit, but their role in generation of fruit flavor volatiles has been unclear. To assess the physiological role of TomloxC in the generation of volatile C6 aldehyde and alcohol fla...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.041608

    authors: Chen G,Hackett R,Walker D,Taylor A,Lin Z,Grierson D

    更新日期:2004-09-01 00:00:00

  • Modulation of an Intracellular Calmodulin-Stimulated Ca2+-Pumping ATPase in Cauliflower by Trypsin (The Use of Calcium Green-5N to Measure Ca2+ Transport in Membrane Vesicles).

    abstract::The effect of controlled trypsin digestion of a calmodulin-stimulated Ca2+-ATPase in low-density intracellular membranes from cauliflower (Brassica oleracea L.) inflorescences was investigated. Ca2+ uptake into vesicles was measured either continuously with the fluorescent Ca2+ indicator Calcium Green-5N or with a rad...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.3.913

    authors: Askerlund P

    更新日期:1996-03-01 00:00:00

  • A novel method of natural cryoprotection : intracellular glass formation in deeply frozen populus.

    abstract::Correlating measurements from differential scanning calorimetry, freeze-fracture freeze-etch electron microscopy, and survival of twigs after two-step cooling experiments, we provide strong evidence that winter-hardened Populus balsamifera v. virginiana (Sarg.) resists the stresses of freezing below -28 degrees C by a...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.79.1.41

    authors: Hirsh AG,Williams RJ,Meryman HT

    更新日期:1985-09-01 00:00:00

  • The abscisic acid induction of a novel peroxidase is antagonized by cytokinin in Spirodela polyrrhiza L.

    abstract::The growth regulator abscisic acid (ABA) can be used to induce dormant bud structures (turions) in the duckweed Spirodela polyrrhiza L. In this paper we show that during this process, ABA rapidly induces elevated levels of mRNA transcripts encoding a novel basic peroxidase. In addition, we show that in the presence of...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.2.497

    authors: Chaloupková K,Smart CC

    更新日期:1994-06-01 00:00:00

  • Light-dependent and tissue-specific expression of the H-protein of the glycine decarboxylase complex.

    abstract::Glycine decarboxylase is a mitochondrial enzyme complex, which is the site of photorespiratory CO2 and NH3 release. Although the proteins that constitute the complex are located within the mitochondria, because of their intimate association with photosynthesis their expression is controlled by light. Comparisons of th...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.1.161

    authors: Srinivasan R,Oliver DJ

    更新日期:1995-09-01 00:00:00

  • Analysis of Porphyra membrane transporters demonstrates gene transfer among photosynthetic eukaryotes and numerous sodium-coupled transport systems.

    abstract::Membrane transporters play a central role in many cellular processes that rely on the movement of ions and organic molecules between the environment and the cell, and between cellular compartments. Transporters have been well characterized in plants and green algae, but little is known about transporters or their evol...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.193896

    authors: Chan CX,Zäuner S,Wheeler G,Grossman AR,Prochnik SE,Blouin NA,Zhuang Y,Benning C,Berg GM,Yarish C,Eriksen RL,Klein AS,Lin S,Levine I,Brawley SH,Bhattacharya D

    更新日期:2012-04-01 00:00:00

  • Elicitation of Necrosis in Vigna unguiculata Walp. by Homogeneous Aspergillus niger Endo-Polygalacturonase and by alpha-d-Galacturonate Oligomers.

    abstract::Endo-polygalacturonase (PG) was purified from a commercial preparation of Aspergillus niger pectinase by means of carboxymethylcellulose chromatography, preparative isoelectric focusing, and gel permeation through Sephadex G-50. The enzyme was electrophoretically homogeneous and consisted of a single polypeptide chain...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.85.3.626

    authors: Cervone F,De Lorenzo G,Degrà L,Salvi G

    更新日期:1987-11-01 00:00:00

  • Gibberellins and heterosis in maize : I. Endogenous gibberellin-like substances.

    abstract::Under controlled environment and/or field conditions, vegetative growth (height, internode length, leaf area, shoot dry weight, grain yield) was greater in an F(1) maize hybrid than in either parental inbred. Endogenous gibberellin (GA)-like substances in apical meristem cylinders were also higher in the hybrid than i...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.71.3.639

    authors: Rood SB,Pharis RP,Koshioka M,Major DJ

    更新日期:1983-03-01 00:00:00

  • Photosynthesis, Rubisco Activity and Amount, and Their Regulation by Transcription in Senescing Soybean Leaves.

    abstract::Senescence is a phase of leaf ontogeny marked by declining photosynthetic activity that, in soybean (Glycine max [L.] Merr.), is paralleled by a decline in chloroplast function. Soybean leaves have different patterns of decline in photosynthetic capacity and chloroplast function associated with nodal position and sink...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.101.1.105

    authors: Jiang CZ,Rodermel SR,Shibles RM

    更新日期:1993-01-01 00:00:00

  • The Arabidopsis Paf1c complex component CDC73 participates in the modification of FLOWERING LOCUS C chromatin.

    abstract::FLOWERING LOCUS C (FLC) is a key repressor of flowering in Arabidopsis (Arabidopsis thaliana) and is regulated, both positively and negatively, by posttranslational histone modifications. For example, vernalization (the promotion of flowering by cold temperatures) epigenetically silences FLC expression through repress...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.158386

    authors: Yu X,Michaels SD

    更新日期:2010-07-01 00:00:00

  • Photosynthetic carbon metabolism in isolated maize bundle sheath strands.

    abstract::Photosynthetically active bundle sheath strands capable of assimilating up to 8 micromoles CO(2) per milligram chlorophyll per hour have been isolated from fully expanded leaves of Zea mays L. Mesophyll cell contamination of the preparations was negligible, as evidenced by light and electron microscopy and by a high r...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.51.4.787

    authors: Chollet R

    更新日期:1973-04-01 00:00:00

  • Enzymic Analysis of Feruloylated Arabinoxylans (Feraxan) Derived from Zea mays Cell Walls I : Purification of Novel Enzymes Capable of Dissociating Feraxan Fragments from Zea mays Coleoptile Cell Wall.

    abstract::Three novel beta-xylan xylanohydrolases capable of dissociating ferulated arabinoxylan (Feraxan) from maize (Zea mays L. hybrid B73 x Mo17) coleoptile sections and two conventional beta-xylan xylanohydrolases (xylanases) were purified from a Bacillus subtilis industrial enzyme preparation (Novo Ban L-120). The Feraxan...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.87.4.883

    authors: Nishitani K,Nevins DJ

    更新日期:1988-08-01 00:00:00

  • Characterization of a molecular modification of phytochrome that is associated with its conversion to the far-red-absorbing form.

    abstract::Phytochrome that has been photoinduced to pellet by irradiation of intact oat (cv. Garry) shoots and recovered from a pellet obtained by centrifugation of crude extracts exhibits modified behavior when compared to soluble phytochrome isolated from shoots that had never been irradiated. This modified behavior includes ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.68.4.789

    authors: Boeshore ML,Pratt LH

    更新日期:1981-10-01 00:00:00

  • Adenylate gradients and Ar:O(2) effects on legume nodules: I. Mathematical models.

    abstract::Mathematical models were developed to test the likelihood that large cytosolic adenylate concentration gradients exist across the bacteria-infected cells of legume nodules. Previous studies hypothesized that this may be the case to account for the unusually low adenylate energy charge (AEC; 0.65) measured in the plant...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.032318

    authors: Wei H,Atkins CA,Layzell DB

    更新日期:2004-02-01 00:00:00

  • Early Events in the Infection of Soybean (Glycine max L. Merr) by Rhizobium japonicum: I. LOCALIZATION OF INFECTIBLE ROOT CELLS.

    abstract::The infectible cells of soybean roots appear to be located at any given time just above the zone of root elongation and just below the position of the smallest emergent root hairs. The location of infectible cells on the primary root at the time of inoculation was inferred from the position of subsequent nodule develo...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.66.6.1027

    authors: Bhuvaneswari TV,Turgeon BG,Bauer WD

    更新日期:1980-12-01 00:00:00

  • Auxin regulation of the gibberellin pathway in pea.

    abstract::The auxin indole-3-acetic acid (IAA) has been shown to promote the biosynthesis of the active gibberellin (GA(1)) in shoots of pea (Pisum sativum). We used northern analysis to investigate the timing of IAA-induced changes in transcript levels of PsGA3ox1 (Mendel's LE), PsGA2ox1, PsGA2ox2, and PsGA20ox1, key genes for...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.010587

    authors: O'Neill DP,Ross JJ

    更新日期:2002-12-01 00:00:00

  • Identification and Partial Characterization of the Denaturation Transition of the Photosystem II Reaction Center of Spinach Chloroplast Membranes.

    abstract::Sensitive differential scanning calorimetry was employed to investigate thylakoid membrane structure. Calorimetric scans of chloroplast membranes suspended in a low ionic strength Hepesbuffered medium revealed endothermic transitions centered at the following temperatures ( degrees C): A (42.5), B (60.6), C(1) (64.9),...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.90.2.575

    authors: Smith KA,Low PS

    更新日期:1989-06-01 00:00:00

  • Different Pathways Act Downstream of the CEP Peptide Receptor CRA2 to Regulate Lateral Root and Nodule Development.

    abstract::C-TERMINALLY ENCODED PEPTIDEs (CEPs) control root system architecture in a non-cell-autonomous manner. In Medicago truncatula, MtCEP1 affects root development by increasing nodule formation and inhibiting lateral root emergence by unknown pathways. Here, we show that the MtCEP1 peptide-dependent increase in nodulation...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.00113

    authors: Mohd-Radzman NA,Laffont C,Ivanovici A,Patel N,Reid D,Stougaard J,Frugier F,Imin N,Djordjevic MA

    更新日期:2016-08-01 00:00:00

  • Comparative Enzymology of the Glyceraldehyde 3-Phosphate Dehydrogenases from Pisum sativum.

    abstract::Glyceraldehyde 3-phosphate dehydrogenases (EC 1.2.1.12 and 1.2.1.13) have been purified from the seed, root, etiolated, and green shoot of peas (Pisum sativum). These enzymes are tetramers of 140,000 daltons, with subunits of 35,000 daltons. The enzymes differ in isoelectric point. The seed enzyme has a pI of 5.1, and...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.54.3.312

    authors: McGowan RE,Gibbs M

    更新日期:1974-09-01 00:00:00

  • beta-d-Glucan Hydrolase Activity in Zea Coleoptile Cell Walls.

    abstract::Enzymes dissociated from corn (hybrid B73 x Mo17) seedling cell walls by solutions of high ionic strength possess the capacity to degrade Avena caryopsis glucan. Inhibitor studies disclosed that both endo- and exoenzyme activities were involved and that the reaction sequence paralleled the autolytic solubilization of ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.65.5.768

    authors: Huber DJ,Nevins DJ

    更新日期:1980-05-01 00:00:00

  • Effects of Membrane ATPase Inhibitors on Light-Induced Phase Shifting of the Circadian Clock in Neurospora crassa.

    abstract::Effects of several membrane ATPase inhibitors on light-induced phase shifting of the circadian conidiation rhythm in Neurospora crassa were examined using mycelial discs in liquid culture. Suppression of phase shifting by the inhibitors was strongly dependent on the pH of the liquid medium in which the discs were cult...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.69.3.619

    authors: Nakashima H

    更新日期:1982-03-01 00:00:00

  • The effect of auxin on stress relaxation in isolated Avena coleoptiles.

    abstract::In order to characterize further the mechanical properties of coleoptile cell walls, stress relaxation measurements were made on methanol-boiled sections of Avena coleoptiles. Relaxation was measured both in mechanically conditioned specimens and in specimens which had not been previously extended. In both cases the r...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.47.6.812

    authors: Cleland R,Haughton PM

    更新日期:1971-06-01 00:00:00

  • Physiological responses of soybean plants grown in a nitrogen-free or energy limited environment.

    abstract::Soybean (Glycine max [L.] Merr.) seedlings grown in the absence of combined N and in an Ar:O(2) (79:21, volume/volume) atmosphere had greater seedling and nodule mass, threefold higher acetylene reducing activity per gram fresh weight nodules, no observable increase in nitrogenase Fe-protein, and a higher energy charg...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.96.1.305

    authors: Zhu YX,Schubert KR,Kohl DH

    更新日期:1991-05-01 00:00:00

  • Inositol Metabolism in Plants. III. Conversion of Myo-inositol-2-H to Cell Wall Polysaccharides in Sycamore (Acer pseudoplatanus L.) Cell Culture.

    abstract::Prolonged growth of cell cultures of sycamore (Acer pseudoplatanus L.) on agar medium containing myo-inositol-2-(3)H resulted in incorporation of label predominately into uronosyl and pentosyl units of cell wall polysaccharides. Procedures normally used to distinguish between pectic substance and hemicellulose yielded...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.41.9.1489

    authors: Roberts RM,Loewus F

    更新日期:1966-11-01 00:00:00