Abstract：
:Thirty monoclonal antibodies produced by mouse hybrid myelomas which react with antigens in hamster or mouse nervous system tissues were derived. Using these antibodies as probes with indirect immunofluorescence and immunoperoxidase techniques, we can selectively identify by morphological criteria many of the structural components of the brain seen at a light-microscopic level, including the neutrophil, neuronal cytoplasm, nuclei, axons, astrocytes and ependyma. Some of the antibodies display cytoskeletal and filamentous structures, including intermediate filaments, microfilaments, neurofilaments, glial and ependymal filaments. The specificity to neural tissue components of these hybridoma antibodies was assessed by their reactivity to mouse and hamster non-neural tissues and selected mouse, hamster, rat and human cultured cell lines. Of the 30 clones analyzed, specificity ranged from 3 clones reacting only with grey matter of mouse and hamster brain, one clone reacting only with axons in animal and human brain, to 19 clones reactive with both neural and non-neural tissue components.
journal_name
J Neuroimmunoljournal_title
Journal of neuroimmunologyauthors
Franko MC,Masters CL,Gibbs CJ Jr,Gajdusek DCdoi
10.1016/0165-5728(81)90019-9subject
Has Abstractpub_date
1981-12-01 00:00:00pages
391-411issue
4eissn
0165-5728issn
1872-8421pii
0165-5728(81)90019-9journal_volume
1pub_type
杂志文章abstract：:Peroxynitrite is formed by the reaction of nitric oxide (NO) and superoxide. Since widespread peroxynitrite activity was observed during experimental allergic encephalomyelitis (EAE), the effect of this strong lipid-peroxidizing agent on myelin integrity was examined. Incubation of myelin suspensions with the peroxyni...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/s0165-5728(98)00239-2
更新日期：1999-03-01 00:00:00
abstract：:Research from our laboratory has demonstrated that the presentation of an aversive conditioned stimulus produces pronounced suppression of several in vitro measures of immune status. The present study was designed to evaluate the role of central corticotropin-releasing hormone (CRH) in the mechanisms mediating these c...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/0165-5728(95)00119-0
更新日期：1995-12-01 00:00:00
abstract：:High titer antibodies primarily of the IgG class were produced against galactocerebroside (GalC) by including keyhole limpet hemocyanin (KLH) and supplemental M. tuberculosis in the adjuvant mixture used for immunization of rabbits. Antibody titers were determined by an ELISA in which microtiter wells were coated with...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/0165-5728(87)90019-1
更新日期：1987-04-01 00:00:00
abstract：:The mechanisms by which autoimmune diseases are triggered and by which the activation of autoreactive T cells is initiated and maintained are not yet fully understood. As the most potent antigen presenting cells (APC), and also being responsible for antigen transport as well as primary sensitisation of T cells, dendri...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章,评审
doi：10.1016/s0165-5728(99)00197-6
更新日期：1999-12-01 00:00:00
abstract：:The aim of this study was to investigate whether chronic stress, induced by repeated daily swimming during 21 days, alters the morphofunctional parameters in the thymus of adult rats. Our results showed that chronic stress reduced thymus mass, total number of thymocytes, volume of the thymus compartments and numerical...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2004.11.002
更新日期：2005-03-01 00:00:00
abstract：:In this study we describe for the first time the production of stable human B cell lines and clones that secrete IgM antibody specific for human myelin basic protein. The technique based on limiting dilutions of Epstein-Barr virus-transformed peripheral B cells from patients with multiple sclerosis, precluded the need...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/0165-5728(89)90092-1
更新日期：1989-09-01 00:00:00
abstract：:To investigate immune mechanisms in the etiology of idiopathic sensory neuronopathy (ISN), we studied neurite outgrowth inhibition and antibody binding to neuronal tissue of serum from 4 patients with ISN. Rat dorsal root ganglion (DRG) cells were cultured in the presence of serum from ISN patients and controls. After...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/s0165-5728(96)00225-1
更新日期：1997-04-01 00:00:00
abstract：:Experimental autoimmune neuritis (EAN) is an inflammatory autoimmune demyelinating disease of the peripheral nervous system (PNS) and represents an animal model of Guillain-Barré syndrome (GBS), which is a major inflammatory demyelinating disease of the PNS in humans. In the present study, the dynamics of the expressi...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/s0165-5728(99)00100-9
更新日期：1999-08-03 00:00:00
abstract：:Hepatocyte growth factor (HGF) limits mouse autoimmune neuroinflammation by promoting the development of tolerogenic dendritic cells (DCs). Given the role played by DCs in the establishment of immunological tolerance, agents that coerce DCs to adopt a protolerogenic function are currently under investigation for multi...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2013.12.004
更新日期：2014-02-15 00:00:00
abstract：:Following inflammation or infection, cytokines are released in the blood. Besides their effect on the immune system, cytokines can also act in the brain to modulate our behaviors, inducing for example anorexia when produced in large amount. This review focuses on our current knowledge on how cytokines can influence th...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章,评审
doi：10.1016/j.jneuroim.2008.04.009
更新日期：2008-07-31 00:00:00
abstract：:The peroxisome proliferator-activated receptor gamma agonist pioglitazone is FDA-approved for treatment of type-2 diabetes due to insulin sensitizing effects. However pioglitazone has anti-inflammatory and neuroprotective effects, reduces glial and T-cell activation, and reduces signs in an animal model of multiple sc...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.jneuroim.2009.04.011
更新日期：2009-06-25 00:00:00
abstract：:Ischemic stroke is one of the leading causes of mortality and morbidity in humans. During brain ischemia and the subsequent reperfusion that occurs with stroke, the generation of the so-called "proinflammatory" prostaglandin E(2) (PGE(2)) increases significantly. Therefore, interest is growing regarding the differenti...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2009.01.015
更新日期：2009-03-31 00:00:00
abstract：:Cytokines such as interleukin-1 (IL-1) are thought to contribute to the inflammatory response associated with autoimmune diseases like multiple sclerosis. We assessed the role of IL-1 in an animal model of MS, experimental allergic encephalomyelitis (EAE), by testing the effects of treatment with an IL-1 receptor anta...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/0165-5728(95)00108-e
更新日期：1995-09-01 00:00:00
abstract：:In this report, we have shown the expression of the complement regulatory proteins decay-accelerating factor (DAF, CD55), membrane cofactor protein (MCP, CD46) and CD59 on human D54-MG astroglioma cells by several methods, including immunofluorescence, flow cytometry and Western blotting and Northern blot analysis. Th...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/0165-5728(93)90022-q
更新日期：1993-09-01 00:00:00
abstract：:This study investigated whether anti-CD40 Ab and 8-oxo-dG attenuate mast cell migration and EAE development. Anti-CD40 Ab and 8-oxo-dG reduced EAE scores, mast cell numbers, expression of adhesion molecules, OX40L and Act1, levels of TNF-α, LTs, expression of cytokines, and co-localization of Treg cells and mast cells...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2013.04.002
更新日期：2013-07-15 00:00:00
abstract：:For the epigenetic characterization of myasthenia gravis (MG), we determined whether long noncoding RNAs (lncRNAs) and messenger RNAs (mRNAs) are expressed differentially in subjects with and without MG. Compared with healthy control subjects, the MG patients had 1561 upregulated lncRNAs, 1034 downregulated lncRNAs, 9...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2016.09.005
更新日期：2016-10-15 00:00:00
abstract：:Immune complexes isolated from sera of patients with multiple sclerosis (MS) were analyzed for their antigenic content. Immune complexes precipitated with polyethylene glycol were inoculated into rabbits. The antisera raised were shown to react to MS and normal brain antigens by crossed immunoelectrophoresis, ELISA an...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/0165-5728(87)90097-x
更新日期：1987-02-01 00:00:00
abstract：:Cell lines of autoimmune T-lymphocytes have been established in several neuroimmunological model diseases and also in a human neurological autoimmune disease, myasthenia gravis. These cell lines generally have the T helper/inducer phenotype and recognize autoantigen in the context of class II histocompatibility antige...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/s0165-5728(85)80018-7
更新日期：1985-08-01 00:00:00
abstract：:Patients with progressive multiple sclerosis (MS) and controls were compared with regard to: (a) in vitro pokeweed mitogen (pwm)-induced IgG secretion, as an indirect measure of T8+ cell-mediated suppressor function; (b) alloantigen-directed cytotoxic activity, a predominantly T8+ cell-mediated function. The MS group ...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/s0165-5728(86)80005-4
更新日期：1986-09-01 00:00:00
abstract：:The majority of human oligodendrocytes in enriched glial cell cultures expresses class I major histocompatibility complex (MHC) antigens. We used a 51Cr release assay to study the susceptibility of oligodendrocyte-enriched glial cells to MHC-restricted and non-restricted immune-mediated cytolysis. Mitogen-activated mo...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/0165-5728(90)90058-u
更新日期：1990-05-01 00:00:00
abstract：:The purposes of this study were to determine plasma and intestinal epinephrine (E) and norepinephrine (NE) concentrations in mice after exercise stress and, the effect of intravenous injection of E and NE (at concentrations during exercise) on viability of intestinal lymphocytes (IL). Exhaustive exercise significantly...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2004.08.008
更新日期：2005-01-01 00:00:00
abstract：:T helper 2 (Th2) cytokines are known to be important in protection against experimental autoimmune encephalomyelitis (EAE). To investigate the role of the signal transducer and activator of transcription factor 6 (STAT6) in EAE we used mice with two different targeted disruptions of the STAT6 gene. In this report, we ...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2008.11.003
更新日期：2009-01-03 00:00:00
abstract：:Tumor necrosis factor-alpha (TNF-alpha) has well recognized effects on cerebral endothelial cells and, therefore, may mediate disruption of blood-brain barrier in patients with multiple sclerosis (MS). To evaluate the in vivo relationship of TNF-alpha to blood-brain barrier impairment in MS, levels of this cytokine in...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/0165-5728(92)90087-2
更新日期：1992-05-01 00:00:00
abstract：:Immunofluorescence studies of sural nerve demonstrated immune reactive C3d and IgM on the surface of myelin sheaths in seven patients with neuropathy and an anti-myelin-associated glycoprotein (MAG) IgM M-protein. Similar deposits of C3d and sometimes IgM were found in four of six patients with acute or chronic inflam...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/0165-5728(88)90101-4
更新日期：1988-06-01 00:00:00
abstract：:Interleukin-10 is a potent immunomodulatory cytokine with possible implications for the pathogenesis of multiple sclerosis. Increased IL10 mRNA expression is associated with stable disease. The interleukin 10 gene is highly polymorphic and certain haplotypes result in differential interleukin-10 expression. The presen...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/s0165-5728(99)00256-8
更新日期：2000-04-03 00:00:00
abstract：:VCAM-1 is an adhesion molecule that is important to leukocyte movement across the blood-brain barrier and is involved in the formation of destructive CNS inflammatory lesions in experimental autoimmune encephalomyelitis (EAE) and multiple sclerosis (MS). We examined VCAM-1 expression in the CNS of animals with passive...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2004.02.012
更新日期：2004-06-01 00:00:00
abstract：:It is widely believed that multiple sclerosis is a T-cell mediated autoimmune disease associated with abnormalities in immunoregulation. This large, prospective study evaluated the lymphocyte immunophenotypic profile of 246 MS patients, divided clinically into a remitting/relapsing group (n = 176) and a progressive gr...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/0165-5728(95)00137-9
更新日期：1995-12-31 00:00:00
abstract：:Interleukin (IL)-10 is an anti-inflammatory cytokine that can down-regulate various aspects of the immune response. In this study we demonstrate that exposure to a psychophysiological stressor (swim stress) increases IL-10 production in female rats in response to an in vivo challenge with bacterial lipopolysaccharide ...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2004.10.016
更新日期：2005-02-01 00:00:00
abstract：:The expression of several cell type-specific markers was studied by indirect immunofluorescence in discontinuous BSA gradient fractionated and unfractionated mouse cerebellar cells cultured for 3 days in vitro from embryonic day 13 through postnatal day 9. Cell surface antigen NS-4 and tetanus toxin receptors are pres...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/0165-5728(81)90024-2
更新日期：1981-12-01 00:00:00
abstract：:Interferon-β (IFN-β) is often discontinued in RRMS patients due to its most common side effect, the flu-like syndrome (FLS). The mechanisms underlying IFN β-induced FLS symptoms are still unclear. The endocannabinoid system (ECS) is a key regulator of pain and inflammation. Thus we tested the hypothesis that the ECS c...
journal_title：Journal of neuroimmunology
pub_type： 杂志文章
doi：10.1016/j.jneuroim.2017.02.007
更新日期：2017-04-15 00:00:00
